LOCUS       Exported               11433 bp ds-DNA     circular SYN 20-AUG-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 19, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11433
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      204..1993
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     protein_bind    2199..2219
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2230..2533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2534..2737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    2803..2827
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2830..6900
                     /codon_start=1
                     /label=CoV-HKU1 S-2P-3C-His8-Strep2x2
                     /translation="MFLIIFILPTTLAVIGDFNCTNSFINDYNKTIPRISEDVVDVSLG
                     LGTYYVLNRVYLNTTLLFTGYFPKSGANFRDLALKGSIYLSTLWYKPPFLSDFNNGIFS
                     KVKNTKLYVNNTLYSEFSTIVIGSVFVNTSYTIVVQPHNGILEITACQYTMCEYPHTVC
                     KSKGSIRNESWHIDSSEPLCLFKKNFTYNVSADWLYFHFYQERGVFYAYYADVGMPTTF
                     LFSLYLGTILSHYYVMPLTCNAISSNTDNETLEYWVTPLSRRQYLLNFDEHGVITNAVD
                     CSSSFLSEIQCKTQSFAPNTGVYDLSGFTVKPVATVYRRIPNLPDCDIDNWLNNVSVPS
                     PLNWERRIFSNCNFNLSTLLRLVHVDSFSCNNLDKSKIFGSCFNSITVDKFAIPNRRRD
                     DLQLGSSGFLQSSNYKIDISSSSCQLYYSLPLVNVTINNFNPSSWNRRYGFGSFNLSSY
                     DVVYSDHCFSVNSDFCPCADPSVVNSCAKSKPPSAICPAGTKYRHCDLDTTLYVKNWCR
                     CSCLPDPISTYSPNTCPQKKVVVGIGEHCPGLGINEEKCGTQLNHSSCFCSPDAFLGWS
                     FDSCISNNRCNIFSNFIFNGINSGTTCSNDLLYSNTEISTGVCVNYDLYGITGQGIFKE
                     VSAAYYNNWQNLLYDSNGNIIGFKDFLTNKTYTILPCYSGRVSAAFYQNSSSPALLYRN
                     LKCSYVLNNISFISQPFYFDSYLGCVLNAVNLTSYSVSSCDLRMGSGFCIDYALPSSGG
                     SGSGISSPYRFVTFEPFNVSFVNDSVETVGGLFEIQIPTNFTIAGHEEFIQTSSPKVTI
                     DCSAFVCSNYAACHDLLSEYGTFCDNINSILNEVNDLLDITQLQVANALMQGVTLSSNL
                     NTNLHSDVDNIDFKSLLGCLGSQCGSSSRSLLEDLLFNKVKLSDVGFVEAYNNCTGGSE
                     IRDLLCVQSFNGIKVLPPILSETQISGYTTAATVAAMFPPWSAAAGVPFSLNVQYRING
                     LGVTMDVLNKNQKLIANAFNKALLSIQNGFTATNSALAKIQSVVNANAQALNSLLQQLF
                     NKFGAISSSLQEILSRLDPPEAQVQIDRLINGRLTALNAYVSQQLSDITLIKAGASRAI
                     EKVNECVKSQSPRINFCGNGNHILSLVQNAPYGLLFIHFSYKPTSFKTVLVSPGLCLSG
                     DRGIAPKQGYFIKQNDSWMFTGSSYYYPEPISDKNVVFMNSCSVNFTKAPFIYLNNSIP
                     NLSDFEAELSLWFKNHTSIAPNLTFNSHINATFLDLYYEMNVIQESIKSLNGSGYIPEA
                     PRDGQAYVRKDGEWVLLSTFLGRSLEVLFQGPGHHHHHHHHSAWSHPQFEKGGGSGGGG
                     SGGSAWSHPQFEK"
     CDS             6754..6777
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6781..6804
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             6811..6834
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6874..6897
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    complement(6899..6923)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6985..7003)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    complement(6986..7003)
                     /label=SP6
     polyA_signal    7029..7253
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7299..7727
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7741..8071
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7922..8057
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8138..8932
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    8987..9359
                     /label=pA
                     /note="SV40 polyA"
     polyA_signal    9106..9227
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9276..9292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9300..9316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9324..9354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9369..9390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9678..10266)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10437..11297)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11298..11402)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatccgcc tttatgtgta actcttggct gaagctctta caccaatgct
       61 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
      121 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
      181 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
      241 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
      301 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
      361 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
      421 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
      481 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
      541 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
      601 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
      661 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
      721 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
      781 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
      841 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
      901 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
      961 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     1021 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     1081 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     1141 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     1201 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     1261 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     1321 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     1381 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     1441 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     1501 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     1561 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     1621 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1681 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1741 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     1801 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     1861 ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag
     1921 tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct
     1981 tgtccttttc ctgctgggga tctcccgatc ccctatggtg cactctcagt acaatctgct
     2041 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     2101 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     2161 atctgcttag ggttaggcgt tttgcgctgc ttcgatcaca actttgtata gaaaagttgg
     2221 ccctgcaggc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2281 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2341 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2401 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2461 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     2521 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     2581 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2641 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     2701 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2761 ctggagacgc catccacgct gttttgacct ccatagaaga caacaacttt gtacaaaaaa
     2821 gttggcacca tgttcctgat cattttcatc ctaccgacta ccctggccgt gatcggagac
     2881 ttcaactgca ctaactcctt tatcaacgac tacaacaaga ctattccgcg catttccgag
     2941 gacgtggtgg acgtgtcact gggcctgggc acttactacg tgctgaaccg ggtgtacctg
     3001 aataccaccc tcctgtttac cggatacttc cctaagtcgg gggcgaactt ccgggatctg
     3061 gctctgaagg gatccatcta cctctctacc ctgtggtaca agccgccgtt cctgtccgac
     3121 ttcaataacg gcatcttcag caaggtcaag aacaccaagc tctacgtgaa caacaccctc
     3181 tactccgagt tctccacaat cgtcattggt tcagtgttcg tgaacactag ctacacaatc
     3241 gtggtgcagc cccataacgg tattctggag atcaccgcct gccaatacac tatgtgcgag
     3301 taccctcaca ccgtgtgtaa atcaaagggc agcatcagaa acgagagctg gcacatcgat
     3361 tccagcgaac ccctctgcct cttcaaaaag aacttcacct acaacgtgtc agccgactgg
     3421 ctctacttcc acttctacca agagcgcgga gtgttctacg cctattacgc cgacgtgggc
     3481 atgcctacta ccttcctctt ctccctgtac ctgggtacta tcctctccca ttactacgtg
     3541 atgccgctga cctgtaacgc gatcagcagc aacactgata atgagacttt ggaatattgg
     3601 gtcacgcctt tgtcgaggcg gcagtatctg ctgaacttcg acgaacacgg cgtgatcacc
     3661 aacgccgtgg actgcagcag ctccttcctg tcggagattc agtgcaagac ccagtccttt
     3721 gcgcctaaca ccggagtgta cgatctctcc ggcttcactg tgaaacctgt ggccaccgtc
     3781 taccgccgca ttcccaacct ccccgattgc gacatcgaca actggctgaa caacgtgtcc
     3841 gtgccatcgc cgctgaactg ggagcggagg atcttctcga actgcaactt caatctgtca
     3901 actctgctgc ggctggtgca cgtggactcc ttttcctgca acaacctgga caagtcgaag
     3961 attttcggat cctgctttaa cagcatcacc gtcgacaagt tcgccatccc gaaccggaga
     4021 agggatgatc ttcagcttgg gtcctccggg ttcttgcagt cctccaacta caagatcgat
     4081 atcagttcct catcctgcca actgtactac tcgttgccgc tcgtgaatgt gaccattaac
     4141 aacttcaacc caagctcctg gaaccggcgc tacggattcg gtagctttaa cctgtcgtcc
     4201 tacgatgtgg tgtactcaga ccactgtttc tcggtcaact ccgacttctg cccatgcgct
     4261 gacccttccg tcgtgaattc ctgcgccaag tcgaagccgc cttcggcgat ttgtcctgct
     4321 ggaaccaagt atcggcactg cgacctggat actactctgt acgtaaagaa ctggtgccgc
     4381 tgctcctgtc tgcccgaccc catctccact tactccccga acacgtgccc ccagaagaag
     4441 gtggtcgtgg gaatcggaga gcactgtcca ggattgggaa ttaacgagga aaaatgcggc
     4501 acccagctga accacagcag ctgcttctgc tcccctgacg cattcctggg atggtccttc
     4561 gattcatgca tttccaacaa ccggtgtaac atattctcca acttcatctt caacgggatc
     4621 aattccggca ccacttgctc gaacgatctg ctatattcca acaccgagat ctccaccggc
     4681 gtctgcgtga actacgacct ctacggcatc acgggtcaag gcatcttcaa ggaagtcagc
     4741 gcggcctact acaacaactg gcagaacttg ctgtacgaca gcaacggaaa catcattggt
     4801 ttcaaggact tcctgactaa caagacctac accatccttc cctgctactc cgggagagtc
     4861 tccgccgcct tctaccaaaa ctcctctagc cccgcccttc tgtatcgcaa cctgaagtgc
     4921 agctacgtcc tcaacaacat atcgttcatc agccagcctt tctactttga ctcctacctg
     4981 ggctgcgtgc tcaacgcagt gaatcttacc tcatattcgg tcagcagctg tgacctccgc
     5041 atgggctcag ggttctgcat cgattacgca ctcccgtcct ccggtggaag cggcagcggc
     5101 atctcatccc cctaccggtt cgtgaccttc gaacccttca acgtgtcctt cgtgaacgac
     5161 tccgtggaaa ccgtcggagg gctgttcgag atccagatcc ctaccaattt caccattgcc
     5221 ggacacgagg aattcatcca aaccagctcg cccaaggtca ccatcgactg ttccgccttt
     5281 gtgtgctcca attacgcggc atgccacgat cttctgtccg aatacggaac cttctgcgat
     5341 aacatcaact ccattctcaa cgaggtcaat gatctgctgg atattaccca gttacaagtg
     5401 gccaacgccc tgatgcaggg cgtgactctg tcctccaacc tgaatactaa cctccattcg
     5461 gatgtggaca acatcgactt caagtcgttg ctgggctgcc tgggaagcca atgcggatcc
     5521 tcctcacggt cgctgctgga agatctgctg tttaacaagg ttaagctgag cgacgtggga
     5581 tttgtggaag cctataacaa ctgtaccggg ggatccgaga tccgggacct cctctgcgtg
     5641 cagtcgttca acggtatcaa ggtcctgccc cctattctga gcgagactca gatttcggga
     5701 tacactaccg ctgcgactgt ggcagctatg ttccctccgt ggtctgccgc tgctggagtg
     5761 ccattctccc tgaacgtcca gtaccgcatc aatggactgg gagtgaccat ggatgtgctg
     5821 aacaagaacc agaagctcat cgccaacgcc ttcaacaagg ccctgctgag catccagaac
     5881 ggattcacgg ccaccaattc cgccctggcc aaaatccaat cagtcgtgaa cgctaacgcc
     5941 caggccctga acagcctgct ccagcagctg ttcaacaagt tcggcgccat ttcctcctcg
     6001 ctccaagaaa ttctgtcacg gctggatccg cctgaagcac aggtccagat tgacagactc
     6061 atcaatgggc gactgactgc cctgaacgcc tacgtcagcc aacagttgag cgacatcacc
     6121 ttgattaagg ccggagcctc cagagctatc gaaaaagtca acgaatgcgt caagtcccag
     6181 tcgccacgga tcaacttctg cggaaatgga aaccatattc tgtccctcgt gcaaaacgcc
     6241 ccgtacggac tgttgttcat ccacttctca tacaagccaa cctcgttcaa gaccgtgctg
     6301 gtgtccccgg gactctgtct ctccggggat cggggaattg cgccgaagca ggggtacttc
     6361 attaagcaga acgactcctg gatgttcaca ggatcttcct actactaccc cgaacccatc
     6421 tccgacaaga acgtcgtgtt tatgaattcg tgcagcgtca actttaccaa ggcccccttc
     6481 atctacttga acaactcgat cccgaacctc agcgacttcg aagccgagct cagcttatgg
     6541 ttcaagaacc acacctccat cgcacccaat ctgaccttca actcgcatat caacgcgacc
     6601 ttcttggacc tttactatga gatgaacgtg atccaggaaa gcatcaagtc ccttaatggc
     6661 tccggctaca tccctgaagc accgagggac ggtcaagcct acgtgcgcaa ggacggggaa
     6721 tgggtgctcc tgtccacgtt cctgggtcgc tccctggagg tgctgttcca gggaccgggg
     6781 catcaccacc accatcatca ccactcagcc tggtcacacc cccaattcga aaagggagga
     6841 ggttcgggcg gtggaggaag cggtggctct gcgtggtccc acccacaatt cgagaagtaa
     6901 tcaactttct tgtacaaagt tgtgaaattc tgcagatatc catcacactg gcggccgctc
     6961 gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
     7021 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     7081 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     7141 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     7201 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     7261 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     7321 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     7381 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     7441 agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     7501 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     7561 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     7621 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc
     7681 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     7741 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag
     7801 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     7861 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     7921 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7981 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     8041 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     8101 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     8161 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     8221 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     8281 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     8341 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     8401 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     8461 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     8521 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     8581 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     8641 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     8701 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     8761 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     8821 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     8881 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     8941 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     9001 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     9061 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     9121 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9181 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     9241 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     9301 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     9361 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     9421 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     9481 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     9541 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9601 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9661 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9721 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9781 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9841 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     9901 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9961 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    10021 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    10081 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
    10141 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    10201 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    10261 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    10321 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    10381 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    10441 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    10501 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    10561 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    10621 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    10681 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    10741 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    10801 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    10861 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10921 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10981 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    11041 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    11101 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    11161 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    11221 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    11281 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    11341 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    11401 cgcacatttc cccgaaaagt gccacctgac gtc
//
