LOCUS       Exported               11466 bp ds-DNA     circular SYN 23-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 19, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11466
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      204..1993
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     protein_bind    2199..2219
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2230..2533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2534..2737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    2803..2827
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2830..6933
                     /codon_start=1
                     /label=CoV-OC43 S-2P-3C-His8-Strep2x2
                     /translation="MFLILLISLPTAFAVIGDLKCPLDSRTGSLNNIDTGPPSISTATV
                     DVTNGLGTYYVLDRVYLNTTLFLNGYYPTSGSTYRNMALKGTDKLSTLWFKPPFLSDFI
                     NGIFAKVKNTKVFKDGVMYSEFPAITIGSTFVNTSYSVVVQPRTINSTQDGVNKLQGLL
                     EVSVCQYNMCEYPHTICHPKLGNHFKELWHMDTGVVSCLYKRNFTYDVNATYLYFHFYQ
                     EGGTFYAYFTDTGVVTKFLFNVYLGMALSHYYVMPLTCISRRDIGFTLEYWVTPLTSRQ
                     YLLAFNQDGIIFNAVDCMSDFMSEIKCKTQSIAPPTGVYELNGYTVQPIADVYRRKPDL
                     PNCNIEAWLNDKSVPSPLNWERKTFSNCNFNMSSLMSFIQADSFTCNNIDAAKIYGMCF
                     SSITIDKFAIPNGRKVDLQLGNLGYLQSFNYRIDTTATSCQLYYNLPAANVSVSRFNPS
                     TWNKRFGFIENSVFKPQPAGVLTNHDVVYAQHCFKAPKNFCPCKLNSSLCVGSGPGKNN
                     GIGTCPAGTNYLTCHNLCNPDPITFTGPYKCPQTKSLVGIGEHCSGLAVKSDYCGGNPC
                     TCQPQAFLGWSADSCLQGDKCNIFANLILHDVNSGLTCSTDLQKANTDIKLGVCVNYDL
                     YGISGQGIFVEVNATYYNSWQNLLYDSNGNLYGFRDYITNRTFMIRSCYSGRVSAAFHA
                     NSSEPALLFRNIKCNYVFNNSLIRQLQPINYFDSYLGCVVNAYNSTAISVQTCDLTVGS
                     GYCVDYSKNGGSGRAITTGYRFTNFEPFTVNSVNDSLEPVGGLYEIQIPSEFTIGNMEE
                     FIQTSSPKVTIDCAAFVCGDYAACKSQLVEYGSFCDNINAILTEVNELLDTTQLQVANS
                     LMNGVTLSTKLKDGVNFNVDDINFSSVLGCLGSECSKASSRSAIEDLLFDKVKLSDVGF
                     VAAYNNCTGGAEIRDLICVQSYKGIKVLPPLLSENQISGYTLAATSASLFPPWTAAAGV
                     PFYLNVQYRINGLGVTMDVLSQNQKLIANAFNNALDAIQEGFDATNSALVKIQAVVNAN
                     AEALNNLLQQLSNRFGAISSSLQEILSRLDPPEAEAQIDRLINGRLTALNAYVSQQLSD
                     STLVKFSAAQAMEKVNECVKSQSSRINFCGNGNHIISLVQNAPYGLYFIHFSYVPTKYV
                     TAKVSPGLCIAGDRGIAPKSGYFVNVNNTWMYTGSGYYYPEPITENNVVVMSTCAVNYT
                     KAPYVMLNTSTPNLPDFREELDQWFKNQTSVAPDLSLDYINVTFLDLQVEMNRLQEAIK
                     VLNGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGRSLEVLFQGPGHHHHHHHHSAWSHPQ
                     FEKGGGSGGGGSGGSAWSHPQFEK"
     CDS             6787..6810
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6814..6837
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             6844..6867
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6907..6930
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    complement(6932..6956)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(7018..7036)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    complement(7019..7036)
                     /label=SP6
     polyA_signal    7062..7286
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7332..7760
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7774..8104
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7955..8090
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8171..8965
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    9020..9392
                     /label=pA
                     /note="SV40 polyA"
     polyA_signal    9139..9260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9309..9325)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9333..9349
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9357..9387)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9402..9423
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9711..10299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10470..11330)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11331..11435)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatccgcc tttatgtgta actcttggct gaagctctta caccaatgct
       61 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
      121 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
      181 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
      241 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
      301 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
      361 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
      421 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
      481 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
      541 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
      601 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
      661 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
      721 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
      781 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
      841 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
      901 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
      961 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     1021 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     1081 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     1141 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     1201 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     1261 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     1321 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     1381 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     1441 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     1501 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     1561 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     1621 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1681 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1741 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     1801 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     1861 ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag
     1921 tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct
     1981 tgtccttttc ctgctgggga tctcccgatc ccctatggtg cactctcagt acaatctgct
     2041 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     2101 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     2161 atctgcttag ggttaggcgt tttgcgctgc ttcgatcaca actttgtata gaaaagttgg
     2221 ccctgcaggc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2281 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2341 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2401 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2461 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     2521 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     2581 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2641 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     2701 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2761 ctggagacgc catccacgct gttttgacct ccatagaaga caacaacttt gtacaaaaaa
     2821 gttggcacca tgttcctgat tctgttaatt tcgctgccga ctgcgtttgc ggtcatcggg
     2881 gacctgaagt gtcccctcga ctcccggacc ggtagcctga acaatattga taccggccct
     2941 ccgtccatct ccaccgcaac cgtcgatgtg accaacggac tcggtaccta ctacgtcctg
     3001 gacagagtct atctgaacac caccctgttc cttaacgggt actaccctac ctccggatcg
     3061 acctaccgga acatggccct gaagggaacc gacaagctgt caaccctgtg gttcaagccg
     3121 ccgtttctta gtgacttcat caacgggatt tttgcgaagg tcaagaacac caaggtgttc
     3181 aaggacggag tgatgtactc cgagttcccc gccattacca ttgggtccac gtttgtcaac
     3241 accagctatt ccgtggttgt gcagcctcgg actatcaaca gcacccagga cggcgtgaat
     3301 aagctgcagg gactcctgga ggtgtccgtg tgtcagtaca acatgtgtga atacccccac
     3361 actatttgcc accccaagct cgggaaccac ttcaaggaac tgtggcacat ggacactggg
     3421 gtggtgtcct gcctgtacaa gcgcaacttc acttacgacg tgaacgccac ttacctgtac
     3481 tttcacttct atcaagaggg aggaaccttc tacgcctact ttactgacac cggcgtggtg
     3541 accaagttcc tgttcaacgt gtacctgggg atggcactgt cccactacta cgtgatgccc
     3601 ctgacgtgca tctcccggcg ggatatcggc ttcactctcg agtactgggt gacccctctg
     3661 acctcacgcc agtacctcct ggccttcaac caggacggca ttatctttaa cgccgtcgat
     3721 tgcatgtctg acttcatgag cgagatcaag tgtaaaaccc agtcgattgc accaccaacc
     3781 ggagtgtacg aactgaacgg atacacggtg caaccaatcg cggatgtgta caggcggaag
     3841 ccagacctcc ccaactgcaa catcgaagcc tggctgaacg acaagtcggt cccttcaccg
     3901 ctcaactggg agcgaaaaac cttctccaat tgcaacttta acatgtcgtc actgatgagc
     3961 ttcatccagg cggactcatt cacttgcaac aacatcgacg ccgccaaaat ctacgggatg
     4021 tgtttctcct ccatcaccat tgacaagttc gccattccta acgggaggaa ggtggacctc
     4081 cagcttggca acctcggcta tctgcagtcc ttcaactacc ggatcgacac caccgccacg
     4141 tcctgccagc tctactataa cctccccgcc gctaacgtgt ccgtgagccg gttcaaccct
     4201 tcaacctgga ataagcgctt cggcttcatc gaaaactcgg tgttcaagcc tcagcctgct
     4261 ggagtgctga ctaatcacga cgtggtgtac gcccagcact gcttcaaggc cccgaagaac
     4321 ttctgcccgt gcaaattgaa ctcgtcgctg tgcgtgggct caggacccgg aaagaacaac
     4381 ggcatcggaa cttgccctgc gggaaccaac tacctgactt gccacaacct gtgcaaccct
     4441 gaccccataa ctttcaccgg tccgtacaag tgtccgcaaa ccaagtccct cgtgggaatt
     4501 ggagagcact gcagcggcct tgccgtgaag tccgattact gcggaggaaa cccctgcaca
     4561 tgccagccgc aggccttcct cggctggtcg gctgacagct gcctgcaggg cgataagtgt
     4621 aacatcttcg ccaacctcat cctgcatgac gtgaactcgg gactcacctg ttccaccgac
     4681 ctccagaagg ctaacaccga tatcaagttg ggagtctgtg tcaactatga cctgtacgga
     4741 atcagcggcc agggcatctt tgtggaggtc aacgccacct actacaactc ctggcagaat
     4801 ctgctgtacg actccaacgg caatctgtac gggttcagag actacattac taatcgcact
     4861 tttatgatcc gctcctgcta ctccggacgc gtgtcagccg cattccatgc caactcctcg
     4921 gaacccgccc tgctgtttcg caacatcaag tgcaactacg tgttcaacaa cagcctcatt
     4981 cggcaactgc agccgatcaa ctactttgat tcgtacctgg gatgtgtcgt gaacgcctac
     5041 aactccaccg ccatctccgt ccaaacttgc gatcttaccg tgggctccgg ctactgcgtg
     5101 gactactcaa agaacggtgg cagcggcaga gccattacca ccggataccg cttcaccaac
     5161 ttcgagcctt tcactgtgaa ctccgtgaat gactccctgg aacccgtcgg cggtctgtac
     5221 gagatccaga tcccgtccga attcacaatc ggcaacatgg aagaattcat ccaaacctcc
     5281 agccccaaag tcactatcga ttgcgccgca ttcgtgtgcg gggactacgc agcgtgcaaa
     5341 tcccaactgg tggaatacgg atccttctgc gacaacatta acgcgatcct gaccgaagtc
     5401 aacgaactgc tggacactac ccagcttcaa gtggccaatt ccctgatgaa cggcgtgact
     5461 ctgagcacca agctgaagga tggcgtcaac ttcaacgtgg atgacataaa cttcagctcc
     5521 gtgctggggt gtttgggttc tgagtgctcc aaggcctcat cccgctccgc cattgaggac
     5581 ctcctgttcg acaaggtcaa gctctccgac gtgggctttg tggccgccta caacaactgt
     5641 accggaggcg ccgagatcag agatttgatc tgcgtccagt cctacaaggg tatcaaggtg
     5701 ctgccgccac tcctcagcga gaaccagatc agcgggtaca ccctcgctgc tacctccgca
     5761 tcgctgttcc cgccgtggac tgcagctgcc ggagtgccat tctacctaaa tgtgcagtac
     5821 cggattaacg gtctgggagt cactatggac gtgctgagcc agaaccagaa gctcatcgcc
     5881 aacgcgttca acaatgcgct ggacgcgatc caggaaggct ttgatgcaac caacagcgca
     5941 ctcgtgaaga tccaggccgt agtgaacgcg aacgctgagg ccctgaacaa cttgctgcag
     6001 cagttgtcaa acagattcgg agccatctcc tcgagcctgc aggaaatcct gtcccggttg
     6061 gatcctcctg aagccgaggc tcaaatcgac cggctcatta acgggaggct gactgccttg
     6121 aacgcttacg tcagccagca attgtccgac tccacgctcg tgaagttctc cgccgcccaa
     6181 gctatggaaa aggtcaacga atgcgtgaag tctcaatcct caagaatcaa cttttgcgga
     6241 aacggcaacc acatcatttc gctggtgcag aacgccccct atggcctgta cttcatccat
     6301 ttctcctacg tgccaaccaa atacgtgact gccaaagtgt cgccgggtct gtgtattgcc
     6361 ggggacaggg gaatcgcccc taagtccgga tacttcgtga acgtgaacaa cacttggatg
     6421 tacaccggca gcggatacta ctaccctgag cccattactg aaaacaacgt cgtcgtgatg
     6481 tccacctgtg ctgtgaacta cactaaggcc ccttacgtga tgctgaacac atccacccct
     6541 aatctgccgg atttcagaga agaactggac cagtggttca agaaccagac cagcgtggcg
     6601 ccagacctct cacttgacta catcaatgtg accttcttgg atctgcaagt ggagatgaat
     6661 cggctccagg aagcgattaa ggtcctgaac gggtctggct atatccccga agccccaagg
     6721 gatggacagg catacgtgcg caaggatgga gaatgggtgc ttttgtcaac ctttttgggt
     6781 cggtccctgg aagtgctgtt ccaagggccg ggtcatcacc accatcacca tcatcactcc
     6841 gcttggagcc accctcagtt cgaaaaggga ggaggatccg gcggtggagg atcgggagga
     6901 tccgcctggt cgcatcccca gttcgagaag taatcaactt tcttgtacaa agttgtgaaa
     6961 ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagg gccctattct
     7021 atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     7081 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     7141 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     7201 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     7261 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     7321 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     7381 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     7441 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg
     7501 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     7561 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     7621 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     7681 ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta
     7741 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     7801 ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     7861 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7921 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     7981 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8041 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8101 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     8161 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     8221 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     8281 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     8341 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     8401 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     8461 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     8521 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     8581 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     8641 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     8701 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     8761 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     8821 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     8881 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     8941 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     9001 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     9061 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     9121 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     9181 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     9241 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     9301 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     9361 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     9421 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     9481 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9541 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9601 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9661 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9721 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9781 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9841 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9901 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9961 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10021 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10081 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10141 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    10201 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10261 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10321 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10381 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    10441 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    10501 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10561 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10621 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10681 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10741 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10801 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10861 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10921 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10981 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    11041 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    11101 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    11161 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    11221 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    11281 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    11341 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    11401 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    11461 gacgtc
//
