LOCUS       Exported               11478 bp ds-DNA     circular SYN 17-AUG-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 19, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11478
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      204..1993
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     protein_bind    2199..2219
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2230..2533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2534..2737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    2803..2827
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2830..6945
                     /codon_start=1
                     /label=CoV-NL63 S-2P-3C-His8-Strep2x2
                     /translation="MKLFLILLVLPLASCFFTCNSNANLSMLQLGVPDNSSTIVTGLLP
                     THWFCANQSTSVYSANGFFYIDVGNHRSAFALHTGYYDANQYYIYVTNEIGLNASVTLK
                     ICKFSRNTTFDFLSNASSSFDCIVNLLFTEQLGAPLGITISGETVRLHLYNVTRTFYVP
                     AAYKLTKLSVKCYFNYSCVFSVVNATVTVNVTTHNGRVVNYTVCDDCNGYTDNIFSVQQ
                     DGRIPNGFPFNNWFLLTNGSTLVDGVSRLYQPLRLTCLWPVPGLKSSTGFVYFNATGSD
                     VNCNGYQHNSVVDVMRYNLNFSANSLDNLKSGVIVFKTLQYDVLFYCSNSSSGVLDTTI
                     PFGPSSQPYYCFINSTINTTHVSTFVGILPPTVREIVVARTGQFYINGFKYFDLGFIEA
                     VNFNVTTASATDFWTVAFATFVDVLVNVSATNIQNLLYCDSPFEKLQCEHLQFGLQDGF
                     YSANFLDDNVLPETYVALPIYYQHTDINFTATASFGGSCYVCKPHQVNISLNGNTSVCV
                     RTSHFSIRYIYNRVKSGSPGDSSWHIYLKSGTCPFSFSKLNNFQKFKTICFSTVEVPGS
                     CNFPLEATWHYTSYTIVGALYVTWSEGNSITGVPYPVSGIREFSNLVLNNCTKYNIYDY
                     VGTGIIRSSNQSLAGGITYVSNSGNLLGFKNVSTGNIFIVTPCNQPDQVAVYQQSIIGA
                     MTAVNESRYGLQNLLQLPNFYYVSNGGNNCTTAVMTYSNFGICADGSLIPVRPRNSSDN
                     GISAIITANLSIPSNWTTSVQVEYLQITSTPIVVDCATYVCNGNPRCKNLLKQYTSACK
                     TIEDALRLSAHLETNDVSSMLTFDSNAFSLANVTSFGDYNLSSVLPQRNIRSSRIAGRS
                     ALEDLLFSKVVTSGLGTVDVDYKSCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYT
                     GSLIGGMVLGGLTSAAAIPFSLALQARLNYVALQTDVLQENQKILAASFNKAINNIVAS
                     FSSVNDAITQTAEAIHTVTIALNKIQDVVNQQGSALNHLTSQLRHNFQAISNSIQAIYD
                     RLDPPQADQQVDRLITGRLAALNAFVSQVLNKYTEVRGSRRLAQQKINECVKSQSNRYG
                     FCGNGTHIFSIVNSAPDGLLFLHTVLLPTDYKNVKAWSGICVDGIYGYVLRQPNLVLYS
                     DNGVFRVTSRIMFQPRLPVLSDFVQIYNCNVTFVNISRVELHTVIPDYVDVNKTLQEFA
                     QNLPKYVKPNFDLTPFNLTYLNLSSELKQLEAKTASLFQTTVELQGLIDQINSTYVDLK
                     LLNRFENGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGRSLEVLFQGPGHHHHHHHHSAW
                     SHPQFEKGGGSGGGGSGGSAWSHPQFEK"
     CDS             complement(3193..3204)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             6799..6822
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6826..6849
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             6856..6879
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6919..6942
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    complement(6944..6968)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(7030..7048)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    complement(7031..7048)
                     /label=SP6
     polyA_signal    7074..7298
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7344..7772
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7786..8116
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7967..8102
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8183..8977
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    9151..9272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9321..9337)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9345..9361
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9369..9399)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9414..9435
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9723..10311)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10482..11342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11343..11447)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatccgcc tttatgtgta actcttggct gaagctctta caccaatgct
       61 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
      121 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
      181 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
      241 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
      301 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
      361 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
      421 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
      481 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
      541 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
      601 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
      661 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
      721 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
      781 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
      841 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
      901 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
      961 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     1021 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     1081 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     1141 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     1201 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     1261 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     1321 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     1381 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     1441 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     1501 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     1561 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     1621 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1681 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1741 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     1801 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     1861 ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag
     1921 tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct
     1981 tgtccttttc ctgctgggga tctcccgatc ccctatggtg cactctcagt acaatctgct
     2041 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     2101 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     2161 atctgcttag ggttaggcgt tttgcgctgc ttcgatcaca actttgtata gaaaagttgg
     2221 ccctgcaggc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2281 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2341 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2401 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2461 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     2521 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     2581 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2641 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     2701 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2761 ctggagacgc catccacgct gttttgacct ccatagaaga caacaacttt gtacaaaaaa
     2821 gttggcacca tgaagctgtt cctgatcctg ctggtgctgc cgctcgcttc ctgcttcttc
     2881 acctgtaaca gcaacgcgaa cctgtccatg ctgcaactcg gggtgcccga taactcctca
     2941 actatcgtga ccggactgtt acccacccac tggttctgcg caaatcagtc gacctccgtg
     3001 tacagcgcga acgggttctt ctacattgat gtcggaaacc atagatccgc cttcgcactg
     3061 cacaccgggt actacgacgc caaccagtat tacatctacg tgaccaacga aatcggtctg
     3121 aatgcctcag tgaccctgaa gatctgcaaa ttctcccgca atactacttt tgattttctg
     3181 agcaacgcat cctcgtcctt cgattgcatc gtgaaccttc tgttcaccga gcaattgggt
     3241 gccccgcttg gaattaccat ctccggagag actgtccggc tgcacctgta caacgtcact
     3301 cgcaccttct acgtgcccgc cgcttacaag ctcactaagc tgagcgtcaa gtgctacttc
     3361 aactactcct gcgtgttctc ggtggtcaac gcaactgtga cggttaacgt caccacccac
     3421 aacggccgcg tggtcaacta cactgtgtgc gacgactgca atggatacac agacaacatt
     3481 ttctcggtgc aacaggacgg acgcattccg aacggattcc ctttcaacaa ctggttcttg
     3541 ctgaccaacg gcagcaccct ggtcgacgga gtgtcgcggc tgtaccagcc actgcgcctg
     3601 acttgcttgt ggccagtgcc tgggctcaag tccagcacag gctttgtgta ctttaacgct
     3661 accggatccg acgtgaactg taacggctac cagcacaact cggtggtgga tgtgatgcgg
     3721 tacaacctta acttctccgc taattccctc gacaacctga agtcgggcgt gatcgtgttt
     3781 aagaccctcc agtacgacgt gctgttctac tgctccaact cgagctccgg agtgctcgac
     3841 acaaccattc ccttcggacc atcaagccag ccctactact gctttatcaa cagcaccatc
     3901 aacaccactc atgtgtccac tttcgtggga atcctgccac cgaccgtcag ggaaatcgtg
     3961 gtggccagga ctggccagtt ctacatcaac ggcttcaagt acttcgacct cggattcatt
     4021 gaggccgtga acttcaatgt cactaccgcg tccgcgactg acttctggac tgtggccttc
     4081 gccaccttcg tggacgtgct ggtcaacgtg tcggccacta acatccagaa cctcctgtac
     4141 tgcgactcac cgttcgagaa gctgcagtgc gagcatctgc agtttggcct gcaagatgga
     4201 ttctactccg ccaacttcct cgacgacaac gtgctccccg aaacctatgt ggccttgcct
     4261 atctattacc aacacaccga catcaacttt accgccaccg cgtcgttcgg aggatcctgc
     4321 tacgtctgca agccccacca ggtcaacatc tccttgaacg gcaatacctc agtgtgcgtg
     4381 cgcacctccc acttctcaat tcggtacatc tacaatagag tgaagtctgg ttcacctggg
     4441 gacagctcat ggcatatcta cctgaagtcc ggcacctgtc cgtttagctt cagcaagctg
     4501 aacaactttc agaagttcaa aaccatctgc ttctccaccg tcgaggtgcc agggagctgt
     4561 aacttcccgc tcgaagccac ctggcactac acctcataca ccatcgtggg agccctttac
     4621 gtgacctggt ctgaaggaaa cagcatcact ggagtgccgt acccagtgtc cggcatcagg
     4681 gagttctcca acctggtgct caacaactgc accaagtaca acatctacga ctacgttggc
     4741 actggcatca tcagatccag caaccagtca ctcgccggtg gcatcaccta cgtgtccaac
     4801 tcgggaaacc tcctgggatt caagaacgtg tccaccggca acatcttcat cgtgaccccc
     4861 tgcaaccagc cggatcaagt cgcggtgtac cagcagtcca tcatcggagc catgacggcc
     4921 gtgaacgaat caagatacgg gttgcaaaat ctcctgcaac tgccgaactt ctactacgtg
     4981 tcgaacggag gaaacaattg cacgactgcc gtcatgacct acagcaactt cggaatttgt
     5041 gccgatgggt ccctgatccc ggtccggcct agaaactcct cggacaacgg catcagcgcg
     5101 atcatcaccg ccaatctgtc cattccatca aactggacca ctagcgtcca ggtcgagtat
     5161 ctgcagatca ccagcacccc gattgtggtg gattgcgcca cttacgtgtg caacgggaat
     5221 ccccgctgca agaacctcct gaagcagtat acttccgcct gcaagactat cgaggatgcc
     5281 ctccgccttt ctgcccactt agaaaccaac gatgtgtcca gcatgctgac cttcgatagc
     5341 aacgctttct cgcttgccaa cgtcacctcc ttcggcgatt acaacctttc ctcggtactg
     5401 ccgcagcgga acattcggtc gtcacggatc gcggggagat cagcgctgga ggacctcctg
     5461 ttctcgaaag tggtcacttc gggactgggg actgtcgacg tggactacaa aagttgcact
     5521 aaggggctct ccattgcgga tcttgcttgc gctcaatact acaacgggat tatggtgctg
     5581 cctggagtgg ccgatgccga aagaatggcc atgtacaccg gctcccttat cggaggaatg
     5641 gtcctcggcg gactcacctc tgccgcggca atcccctttt ccctggcctt gcaagcccgc
     5701 ctgaactacg tggctctgca gactgacgtg ctgcaggaga accagaagat tctggccgcc
     5761 tcattcaaca aggccatcaa caacatcgtg gcctccttct cctccgtgaa tgatgcgatt
     5821 acccagaccg ccgaagccat ccacactgtc acgattgccc tgaacaagat tcaggatgtc
     5881 gtgaatcagc aaggctcagc cctgaatcac ctgacttcgc aattgcggca caacttccaa
     5941 gcgatctcga acagcatcca ggccatctac gacagactgg atcctccgca agccgaccaa
     6001 caggtcgacc ggctgattac aggacgcctc gcagcactca acgccttcgt gagccaggtc
     6061 ttgaacaagt acactgaagt gcggggatca agacgcctgg cgcagcagaa gattaacgag
     6121 tgcgtcaaga gccagtccaa ccgatacggt ttttgcggga acggcaccca catcttctcc
     6181 attgtgaata gcgcaccgga tggcctcctg ttccttcata ccgtgctgct gcctaccgac
     6241 tacaagaacg tgaaggcttg gagcgggatc tgtgtcgacg gcatctacgg ttacgtgctg
     6301 aggcagccga acctggtgtt gtacagcgac aacggagtgt tcagagtgac gtcccggatc
     6361 atgtttcaac caagacttcc tgtgctctcc gacttcgtgc agatatacaa ctgtaacgtg
     6421 accttcgtga acatttcccg cgtggaactc cacaccgtga ttcctgacta cgtggacgtg
     6481 aacaagaccc tgcaggagtt cgcgcagaat ctccctaaat acgtgaagcc caacttcgac
     6541 ctgaccccct ttaacctgac atacctcaac ctgtcctccg aattgaagca gctggaggcc
     6601 aagaccgcct cgctgttcca aaccaccgtg gagctgcagg gcctgatcga ccagattaat
     6661 tccacctacg tcgacctgaa gctgctgaac cgcttcgaga acggctccgg ctacatcccg
     6721 gaagctcccc gagatgggca ggcctacgtc cggaaggacg gagaatgggt cctcctctcc
     6781 accttcctgg gccgctcgct agaagtgctg ttccaagggc cgggacacca tcaccaccac
     6841 catcatcatt cggcatggtc gcaccctcaa ttcgagaagg gcggaggcag tggaggtgga
     6901 ggttccggcg gatccgcttg gtcccacccg caattcgaaa agtaatcaac tttcttgtac
     6961 aaagttgtga aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga
     7021 gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc
     7081 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     7141 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     7201 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     7261 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     7321 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     7381 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     7441 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7501 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     7561 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7621 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7681 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa
     7741 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     7801 tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta
     7861 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7921 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     7981 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8041 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     8101 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     8161 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     8221 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     8281 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     8341 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     8401 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     8461 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     8521 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     8581 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     8641 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     8701 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     8761 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     8821 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     8881 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     8941 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     9001 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     9061 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     9121 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     9181 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     9241 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     9301 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     9361 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     9421 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     9481 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     9541 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     9601 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9661 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9721 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9781 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9841 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9901 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9961 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    10021 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    10081 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    10141 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
    10201 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    10261 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    10321 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    10381 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    10441 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    10501 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    10561 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    10621 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    10681 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    10741 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    10801 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    10861 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    10921 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    10981 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    11041 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    11101 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    11161 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    11221 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    11281 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    11341 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    11401 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    11461 aaagtgccac ctgacgtc
//
