LOCUS Exported 8216 bp ds-DNA circular SYN 20-SEP-2018 DEFINITION ATCC 37254: very usable general purpose vector. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8216) AUTHORS . TITLE Direct Submission JOURNAL Exported Wednesday, Jan 20, 2021 from SnapGene Viewer 5.0.8 https://www.snapgene.com COMMENT http://www.informaxinc.com/ FEATURES Location/Qualifiers source 1..8216 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 422..1013 /label=CMV /note="/vntifkey=29" primer_bind 875..895 /label=4684 misc_feature 1228..1278 /label=NLS /label=NLS(1) /note="NLS" misc_feature join(1279..1983,2004..2183,2229..2883,2904..3395,3437..4090, 4111..4712,4763..4940,4961..5379) /label=hSpCsn1 /note="hSpCsn1" misc_feature 1984..2003 /label=hSpCsn1 /label=hSpCsn1(7) /note="hSpCsn1" primer_bind 1984..2003 /label=YJ625F misc_feature 2184..2228 /label=hSpCsn1 /label=hSpCsn1(4) /note="hSpCsn1" primer_bind complement(2184..2208) /label=No.14 primer_bind 2209..2228 /label=No.14 /label=No.14(1) misc_feature 2884..2903 /label=hSpCsn1 /label=hSpCsn1(6) /note="hSpCsn1" primer_bind 2884..2903 /label=YJ626F misc_feature 3396..3436 /label=hSpCsn1 /label=hSpCsn1(1) /note="hSpCsn1" primer_bind complement(3396..3417) /label=No.17 /label=No.17(1) primer_bind 3418..3436 /label=No.17 misc_feature 4091..4110 /label=hSpCsn1 /label=hSpCsn1(5) /note="hSpCsn1" primer_bind 4091..4110 /label=YJ627F misc_feature 4713..4762 /label=hSpCsn1 /label=hSpCsn1(2) /note="hSpCsn1" primer_bind complement(4713..4734) /label=No.21 primer_bind 4735..4762 /label=No.21 /label=No.21(1) misc_feature 4941..4960 /label=hSpCsn1 /label=hSpCsn1(3) /note="hSpCsn1" primer_bind 4941..4960 /label=YJ628F misc_feature 5380..5427 /label=NLS /note="NLS" misc_RNA 5449..5467 /label=MS2 stem loop /note="stem loop that binds the bacteriophage MS2 coat protein" misc_RNA 5488..5506 /label=MS2 stem loop /label=MS2 stem loop(3) /note="stem loop that binds the bacteriophage MS2 coat protein" misc_RNA 5558..5576 /label=MS2 stem loop /label=MS2 stem loop(1) /note="stem loop that binds the bacteriophage MS2 coat protein" misc_RNA 5597..5615 /label=MS2 stem loop /label=MS2 stem loop(4) /note="stem loop that binds the bacteriophage MS2 coat protein" misc_RNA 5667..5685 /label=MS2 stem loop /label=MS2 stem loop(2) /note="stem loop that binds the bacteriophage MS2 coat protein" misc_RNA 5706..5724 /label=MS2 stem loop /label=MS2 stem loop(5) /note="stem loop that binds the bacteriophage MS2 coat protein" terminator complement(5760..5879) /label=SV40_PA_terminator primer_bind complement(5860..5879) /label=YJ638R primer_bind complement(6024..6043) /label=0721 promoter complement(6037) /label=P(LAC) /note="lac promoter" /note="/vntifkey=30" rep_origin 6397 /label=ORI /note="RNaseH cleavage point" /note="/vntifkey=33" CDS complement(7159..8016) /label=AP(R) /note="bla gene- Ap(r) determinant" /note="/vntifkey=4" promoter complement(8051) /label=P(BLA) /note="bla gene promoter" /note="/vntifkey=30" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagcttgca tgcctgcagg 421 tcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat 481 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc 541 aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc 601 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt 661 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta 721 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg 781 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac 841 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg 901 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac 961 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccggactc 1021 tagaggatcc ggtactcgag gaactgaaaa accagaaagt taactggtaa gtttagtctt 1081 tttgtctttt atttcaggtc ccgatccggt ggtggtgcaa atcaaagaac tgctcctcag 1141 tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct aaaagctgcg 1201 gaattgtacc cgcggacgcg tgccaccatg gccccaaaga agaagcggaa ggtcggtatc 1261 cacggagtcc cagcagccga caagaagtac agcatcggcc tggacatcgg caccaactct 1321 gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg 1381 ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc 1441 ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg 1501 aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac 1561 agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg 1621 caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc 1681 taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat 1741 ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac 1801 cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg 1861 ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga 1921 ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat 1981 ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac 2041 ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg 2101 gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac 2161 ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc 2221 cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg 2281 aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc 2341 aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc 2401 atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga 2461 gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac 2521 ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac 2581 aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg 2641 gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc 2701 tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg 2761 accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac 2821 gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga 2881 aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc 2941 aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc 3001 gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac 3061 gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt 3121 ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg 3181 ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga 3241 tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc 3301 ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag 3361 ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc 3421 cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag 3481 ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag 3541 cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag 3601 aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc 3661 ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac 3721 ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac 3781 tacgatgtgg accatatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag 3841 gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc 3901 gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga 3961 aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc 4021 ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg 4081 gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg 4141 atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg 4201 cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc 4261 gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg 4321 tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag 4381 tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc 4441 gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat 4501 aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg 4561 aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac 4621 agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac 4681 agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag 4741 aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag 4801 aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc 4861 atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc 4921 tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc 4981 ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa 5041 cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag 5101 ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac 5161 aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg 5221 accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg 5281 tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg 5341 tacgagacac ggatcgacct gtctcagctg ggaggcgaca aaaggccggc ggccacgaaa 5401 aaggccggcc aggcaaaaaa gaaaaagtaa accggtcttg cagcacatac atgaggatca 5461 cccatgtctg caggtcgact ctagaaaaca tgaggatcac ccatgtctgc agtattcccg 5521 ggttcattag atcctaaggt acctaattgc ctagaaaaca tgaggatcac ccatgtctgc 5581 aggtcgactc tagaaaacat gaggatcacc catgtctgca gtattcccgg gttcattaga 5641 tcctaaggta cctaattgcc tagaaaacat gaggatcacc catgtctgca ggtcgactct 5701 agaaaacatg aggatcaccc atgtgtgcac cttaagcttg gcccgcgggg atccagacat 5761 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt 5821 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca 5881 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt 5941 tttttcggat cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata 6001 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 6061 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 6121 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 6181 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 6241 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 6301 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 6361 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 6421 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 6481 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 6541 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 6601 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 6661 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 6721 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 6781 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 6841 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 6901 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 6961 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 7021 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 7081 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 7141 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 7201 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 7261 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 7321 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 7381 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 7441 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 7501 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 7561 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 7621 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 7681 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 7741 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 7801 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 7861 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 7921 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 7981 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 8041 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 8101 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 8161 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc //