LOCUS       Exported                8216 bp ds-DNA     circular SYN 20-SEP-2018
DEFINITION  ATCC 37254: very usable general purpose vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 20, 2021 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
COMMENT     http://www.informaxinc.com/
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        422..1013
                     /label=CMV
                     /note="/vntifkey=29"
     primer_bind     875..895
                     /label=4684
     misc_feature    1228..1278
                     /label=NLS
                     /label=NLS(1)
                     /note="NLS"
     misc_feature    join(1279..1983,2004..2183,2229..2883,2904..3395,3437..4090,
                     4111..4712,4763..4940,4961..5379)
                     /label=hSpCsn1
                     /note="hSpCsn1"
     misc_feature    1984..2003
                     /label=hSpCsn1
                     /label=hSpCsn1(7)
                     /note="hSpCsn1"
     primer_bind     1984..2003
                     /label=YJ625F
     misc_feature    2184..2228
                     /label=hSpCsn1
                     /label=hSpCsn1(4)
                     /note="hSpCsn1"
     primer_bind     complement(2184..2208)
                     /label=No.14
     primer_bind     2209..2228
                     /label=No.14
                     /label=No.14(1)
     misc_feature    2884..2903
                     /label=hSpCsn1
                     /label=hSpCsn1(6)
                     /note="hSpCsn1"
     primer_bind     2884..2903
                     /label=YJ626F
     misc_feature    3396..3436
                     /label=hSpCsn1
                     /label=hSpCsn1(1)
                     /note="hSpCsn1"
     primer_bind     complement(3396..3417)
                     /label=No.17
                     /label=No.17(1)
     primer_bind     3418..3436
                     /label=No.17
     misc_feature    4091..4110
                     /label=hSpCsn1
                     /label=hSpCsn1(5)
                     /note="hSpCsn1"
     primer_bind     4091..4110
                     /label=YJ627F
     misc_feature    4713..4762
                     /label=hSpCsn1
                     /label=hSpCsn1(2)
                     /note="hSpCsn1"
     primer_bind     complement(4713..4734)
                     /label=No.21
     primer_bind     4735..4762
                     /label=No.21
                     /label=No.21(1)
     misc_feature    4941..4960
                     /label=hSpCsn1
                     /label=hSpCsn1(3)
                     /note="hSpCsn1"
     primer_bind     4941..4960
                     /label=YJ628F
     misc_feature    5380..5427
                     /label=NLS
                     /note="NLS"
     misc_RNA        5449..5467
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        5488..5506
                     /label=MS2 stem loop
                     /label=MS2 stem loop(3)
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        5558..5576
                     /label=MS2 stem loop
                     /label=MS2 stem loop(1)
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        5597..5615
                     /label=MS2 stem loop
                     /label=MS2 stem loop(4)
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        5667..5685
                     /label=MS2 stem loop
                     /label=MS2 stem loop(2)
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        5706..5724
                     /label=MS2 stem loop
                     /label=MS2 stem loop(5)
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     terminator      complement(5760..5879)
                     /label=SV40_PA_terminator
     primer_bind     complement(5860..5879)
                     /label=YJ638R
     primer_bind     complement(6024..6043)
                     /label=0721
     promoter        complement(6037)
                     /label=P(LAC)
                     /note="lac promoter"
                     /note="/vntifkey=30"
     rep_origin      6397
                     /label=ORI
                     /note="RNaseH cleavage point"
                     /note="/vntifkey=33"
     CDS             complement(7159..8016)
                     /label=AP(R)
                     /note="bla gene- Ap(r) determinant"
                     /note="/vntifkey=4"
     promoter        complement(8051)
                     /label=P(BLA)
                     /note="bla gene promoter"
                     /note="/vntifkey=30"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagcttgca tgcctgcagg
      421 tcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      481 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      541 aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      601 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      661 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      721 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      781 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      841 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      901 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
      961 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccggactc
     1021 tagaggatcc ggtactcgag gaactgaaaa accagaaagt taactggtaa gtttagtctt
     1081 tttgtctttt atttcaggtc ccgatccggt ggtggtgcaa atcaaagaac tgctcctcag
     1141 tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct aaaagctgcg
     1201 gaattgtacc cgcggacgcg tgccaccatg gccccaaaga agaagcggaa ggtcggtatc
     1261 cacggagtcc cagcagccga caagaagtac agcatcggcc tggacatcgg caccaactct
     1321 gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg
     1381 ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc
     1441 ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg
     1501 aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac
     1561 agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg
     1621 caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc
     1681 taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat
     1741 ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac
     1801 cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg
     1861 ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga
     1921 ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat
     1981 ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac
     2041 ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg
     2101 gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac
     2161 ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc
     2221 cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg
     2281 aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc
     2341 aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc
     2401 atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga
     2461 gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac
     2521 ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac
     2581 aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg
     2641 gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc
     2701 tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg
     2761 accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac
     2821 gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga
     2881 aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc
     2941 aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc
     3001 gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac
     3061 gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt
     3121 ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg
     3181 ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga
     3241 tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc
     3301 ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag
     3361 ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc
     3421 cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag
     3481 ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag
     3541 cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag
     3601 aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc
     3661 ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac
     3721 ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac
     3781 tacgatgtgg accatatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag
     3841 gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc
     3901 gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga
     3961 aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc
     4021 ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg
     4081 gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg
     4141 atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg
     4201 cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc
     4261 gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg
     4321 tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag
     4381 tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc
     4441 gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat
     4501 aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg
     4561 aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac
     4621 agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac
     4681 agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag
     4741 aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag
     4801 aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc
     4861 atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc
     4921 tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc
     4981 ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa
     5041 cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag
     5101 ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac
     5161 aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg
     5221 accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg
     5281 tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg
     5341 tacgagacac ggatcgacct gtctcagctg ggaggcgaca aaaggccggc ggccacgaaa
     5401 aaggccggcc aggcaaaaaa gaaaaagtaa accggtcttg cagcacatac atgaggatca
     5461 cccatgtctg caggtcgact ctagaaaaca tgaggatcac ccatgtctgc agtattcccg
     5521 ggttcattag atcctaaggt acctaattgc ctagaaaaca tgaggatcac ccatgtctgc
     5581 aggtcgactc tagaaaacat gaggatcacc catgtctgca gtattcccgg gttcattaga
     5641 tcctaaggta cctaattgcc tagaaaacat gaggatcacc catgtctgca ggtcgactct
     5701 agaaaacatg aggatcaccc atgtgtgcac cttaagcttg gcccgcgggg atccagacat
     5761 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     5821 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     5881 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     5941 tttttcggat cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata
     6001 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6061 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6121 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6181 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6241 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6301 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6361 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6421 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6481 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6541 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6601 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6661 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6721 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6781 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     6841 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6901 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     6961 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7021 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7081 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7141 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7201 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7261 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7321 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7381 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7441 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7501 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7561 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7621 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7681 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7741 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7801 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7861 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     7921 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7981 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8041 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8101 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     8161 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
