LOCUS       pNSU299_CPR1_2         11752 bp ds-DNA     circular     21-JAN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     gene            complement(9528..10202)
                     /label="TRP1"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      8976..9431
                     /label="f1 ori"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1118..1202
                     /label="RNAse P leader sequence"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1119..1137
                     /label="TetO"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1223..1304
                     /label="sgRNA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     terminator      3148..3545
                     /label="tGPM1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_binding    3848..3870
                     /label="LacO"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4356..4378
                     /label="kozak rev"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        791..1202
                     /label="pRPR1"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    8501..8515
                     /label="NLS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        1757..2516
                     /label="pGPM1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      8538..8777
                     /label="CYC1_terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      71..753
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             8903..8961
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    8495..8515
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1118..1118
                     /label="TSS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             2524..3147
                     /label="TetR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     gene            10668..11728
                     /label="Ampicillin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3949..4355
                     /label="TEF1 promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     gene            complement(10203..10483)
                     /label="TRP1 prom"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     gene            4379..8494
                     /label="SpCas9"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      1311..1748
                     /label="RPR1t"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(1310..1334)
                     /label="RPR1-rev"
                     /note="sequence: gattaaattggaagttggatatggc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(8832..8854)
                     /label="M13F43"
                     /note="sequence: AGGGTTTTCCCAGTCACGACGTT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          1009..1030
                     /label="RPR1-fwd"
                     /note="sequence: caggatatgttaacgacccaca"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          3913..3932
                     /label="T3"
                     /note="sequence: AATTAACCCTCACTAAAGGG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(8906..8927)
                     /label="pNSU299-3546-REV"
                     /note="sequence: tgcgggcctcttcgctattacg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          8836..8857
                     /label="pNSU299-3455-FWD"
                     /note="sequence: tcgtgactgggaaaaccctggc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          3809..3834
                     /label="pexR"
                     /note="sequence: CAGGCTTTACACTTTATGCTTCCGGC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          3856..3879
                     /label="M13R49"
                     /note="sequence: GAGCGGATAACAATTTCACACAGG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(10563..10583)
                     /label="pexF"
                     /note="sequence: GGAGCAGACAAGCCCGTCAGG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(3653..3674)
                     /label="pNSU299-3674-REV"
                     /note="sequence: gctcttccgcttcctcgctcac"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          9349..9366
                     /label="pEGFP-C1-2800-R"
                     /note="sequence: GATTTCGGCCTATTGGTT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(8815..8832)
                     /label="M13F"
                     /note="sequence: TGTAAAACGACGGCCAGT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(10726..10745)
                     /label="VF2"
                     /note="sequence: TGCCACCTGACGTCTAAGAA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(8547..8565)
                     /label="CYC1Reverse"
                     /note="sequence: GCGTGAATGTAAGCGTGAC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          10621..10639
                     /label="pGexR"
                     /note="sequence: GAGCTGCATGTGTCAGAGG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(1223..1262)
                     /label="67-3'gRNA-extender"
                     /note="sequence: gactagccttattttaacttgctatttctagctctaaaac"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          3876..3893
                     /label="M13R"
                     /note="sequence: CAGGAAACAGCTATGACC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          3590..3609
                     /label="VR"
                     /note="sequence: ATTACCGCCTTTGAGTGAGC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(8786..8805)
                     /label="T7"
                     /note="sequence: TAATACGACTCACTATAGGG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          1163..1202
                     /label="68-5'gRNA-extender"
                     /note="sequence: tcgcggctgggaacgaaactctgggagctgcgattggcag"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(10667..10688)
                     /label="pQEfPR"
                     /note="sequence: GTATCACGAGGCCCTTTCGTCT"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
       61 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
      121 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
      181 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
      241 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
      301 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
      361 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      421 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      481 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      541 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      601 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      661 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      721 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      781 ccttttgctc gggggatctg ccaattgaac ataacatggt agttacatat actagtaata
      841 tggttcggca cacattaaaa gtataaaaac tatctgaatt acgaattaca tatattggtc
      901 ataaaaatca atcaatcatc gtgtgtttta tatgtctctt atctaagtat aagaatatcc
      961 atagttaata ttcacttacg ctacctttta acctgtaatc attgtcaaca ggatatgtta
     1021 acgacccaca ttgataaacg ctagtatttc tttttcctct tcttattggc cggctgtctc
     1081 tatactcccc tatagtctgt ttcttttcgt ttcgattgtc cctatcagtg atagagatgg
     1141 cgcacatggt acgctgtggt gctcgcggct gggaacgaaa ctctgggagc tgcgattggc
     1201 agCTTAACAG GCATAACCTA GGgttttaga gctagaaata gcaagttaaa ataaggctag
     1261 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttctcgag ccatatccaa
     1321 cttccaattt aatctttctt ttttaatttt cacttatttg cgatacagaa agaaaaaagc
     1381 gatagtaact attgaatttt gtttggattt ggttagatta gatatggttt ctctttatat
     1441 ttacatgcta aaaatgggct acaccagaga tacataatta gatatatata cgccagtaca
     1501 ccttatcggc ccaagccttg tcccaaggca gcgttttgtt cttggaaacg ctgccctaca
     1561 cgttcgctat gcttcaagaa cttttctgag cacttcatga tgcatgtttg ttccttattg
     1621 gttagctttg atgttgtgaa gtcattgaca cagtctgtga aacatctttc taccagatta
     1681 gagtacaaac gcatgaaatc cttcatttgc ttttgttcca ctactttttg gaactcttgt
     1741 tgttctttCC TGCAGGtagt cgtgcaatgt atgactttaa gatttgtgag caggaagaaa
     1801 agggagaatc ttctaacgat aaacccttga aaaactgggt agactacgct atgttgagtt
     1861 gctacgcagg ctgcacaatt acacgagaat gctcccgcct aggatttaag gctaagggac
     1921 gtgcaatgca gacgacagat ctaaatgacc gtgtcggtga agtgttcgcc aaacttttcg
     1981 gttaacacat gcagtgatgc acgcgcgatg gtgctaagtt acatatatat atatatatat
     2041 atatatatat atatatagcc atagtgatgt ctaagtaacc tttatggtat atttcttaat
     2101 gtggaaagat actagcgcgc gcacccacac acaagcttcg tcttttcttg aagaaaagag
     2161 gaagctcgct aaatgggatt ccactttccg ttccctgcca gctgatggaa aaaggttagt
     2221 ggaacgatga agaataaaaa gagagatcca ctgaggtgaa atttcagctg acagcgagtt
     2281 tcatgatcgt gatgaacaat ggtaacgagt tgtggctgtt gccagggagg gtggttctca
     2341 acttttaatg tatggccaaa tcgctacttg ggtttgttat ataacaaaga agaaataatg
     2401 aactgattct cttcctcctt cttgtccttt cttaattctg ttgtaattac cttcctttgt
     2461 aatttttttt gtaattattc ttcttaataa tccaaacaaa cacacatatt acaatatatt
     2521 aaaatgtcta gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag
     2581 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct
     2641 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg
     2701 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta
     2761 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta
     2821 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt
     2881 ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat
     2941 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa
     3001 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac
     3061 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa
     3121 caacttaaat gtgaaagtgg gtcttaagtc tgaagaatga atgatttgat gatttctttt
     3181 tccctccatt tttcttactg aatatatcaa tgatatagac ttgtatagtt tattatttca
     3241 aattaagtag ctatatatag tcaagataac gtttgtttga cacgattaca ttattcgtcg
     3301 acatcttttt tcagcctgtc gtggtagcaa tttgaggagt attattaatt gaataggttc
     3361 attttgcgct cgcataaaca gttttcgtca gggacagtat gttggaatga gtggtaatta
     3421 atggtgacat gacatgttat agcaataacc ttgatgttta catcgtagtt taatgtacac
     3481 cccgcgaatt cgttcaagta ggagtgcacc aattgcaaag ggaaaagctg aatgggcagt
     3541 tcgaaCCGCG Gtctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3601 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     3661 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     3721 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     3781 tgtgagttac ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcctat
     3841 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     3901 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggAGCTCA TAGCTTCAAA
     3961 ATGTTTCTAC TCCTTTTTTA CTCTTCCAGA TTTTCTCGGA CTCCGCGCAT CGCCGTACCA
     4021 CTTCAAAACA CCCAAGCACA GCATACTAAA TTTCCCCTCT TTCTTCCTCT AGGGTGTCGT
     4081 TAATTACCCG TACTAAAGGT TTGGAAAAGA AAAAAGAGAC CGCCTCGTTT CTTTTTCTTC
     4141 GTCGAAAAAG GCAATAAAAA TTTTTATCAC GTTTCTTTTT CTTGAAAATT TTTTTTTTGA
     4201 TTTTTTTCTC TTTCGATGAC CTCCCATTGA TATTTAAGTT AATAAACGGT CTTCAATTTC
     4261 TCAAGTTTCA GTTTCATTTT TCTTGTTCTA TTACAACTTT TTTTACTTCT TGCTCATTAG
     4321 AAAGAAAGCA TAGCAATCTA ATCTAAGTTT TCTAGAACTA GTGGATCCCC CGGGaaaaAT
     4381 GGACAAGAAG TACTCCATTG GGCTCGATAT CGGCACAAAC AGCGTCGGtT GGGCCGTCAT
     4441 TACGGACGAG TACAAGGTGC CGAGCAAAAA ATTCAAAGTT CTGGGCAATA CCGATCGCCA
     4501 CAGCATAAAG AAGAACCTCA TTGGCGCCCT CCTGTTCGAC TCCGGGGAGA CGGCCGAAGC
     4561 CACGCGGCTC AAAAGAACAG CACGGCGCAG ATATACCCGC AGAAAGAATC GGATCTGCTA
     4621 CCTGCAGGAG ATCTTTAGTA ATGAGATGGC TAAGGTGGAT GACTCTTTCT TCCATAGGCT
     4681 GGAGGAGTCC TTTTTGGTGG AGGAGGATAA AAAGCACGAG CGCCACCCAA TCTTTGGCAA
     4741 TATCGTGGAC GAGGTGGCGT ACCATGAAAA GTACCCAACC ATATATCATC TGAGGAAGAA
     4801 GCTTGTAGAC AGTACTGATA AGGCTGACTT GCGGTTGATC TATCTCGCGC TGGCGCATAT
     4861 GATCAAATTT CGGGGACACT TCCTCATCGA GGGGGACCTG AACCCAGACA ACAGCGATGT
     4921 CGACAAACTC TTTATCCAAC TGGTTCAGAC TTACAATCAG CTTTTCGAAG AGAACCCGAT
     4981 CAACGCATCC GGAGTTGACG CCAAAGCAAT CCTGAGCGCT AGGCTGTCCA AATCCCGGCG
     5041 GCTCGAAAAC CTCATCGCAC AGCTCCCTGG GGAGAAGAAG AACGGCCTGT TTGGTAATCT
     5101 TATCGCCCTG TCACTCGGGC TGACCCCCAA CTTTAAATCT AACTTCGACC TGGCCGAAGA
     5161 TGCCAAGCTT CAACTGAGCA AAGACACCTA CGATGATGAT CTCGACAATC TGCTGGCCCA
     5221 GATCGGCGAC CAGTACGCAG ACCTTTTTTT GGCGGCAAAG AACCTGTCAG ACGCCATTCT
     5281 GCTGAGTGAT ATTCTGCGAG TGAACACGGA GATCACCAAA GCTCCGCTGA GCGCTAGTAT
     5341 GATCAAGCGC TATGATGAGC ACCACCAAGA CTTGACTTTG CTGAAGGCCC TTGTCAGACA
     5401 GCAACTGCCT GAGAAGTACA AGGAAATTTT CTTCGATCAG TCTAAAAATG GCTACGCCGG
     5461 ATACATTGAC GGCGGAGCAA GCCAGGAGGA ATTTTACAAA TTTATTAAGC CCATCTTGGA
     5521 AAAAATGGAC GGCACCGAGG AGCTGCTGGT AAAGCTTAAC AGAGAAGATC TGTTGCGCAA
     5581 ACAGCGCACT TTCGACAATG GAAGCATCCC CCACCAGATT CACCTGGGCG AACTGCACGC
     5641 TATCCTCAGG CGGCAAGAGG ATTTCTACCC CTTTTTGAAA GATAACAGGG AAAAGATTGA
     5701 GAAAATCCTC ACATTTCGGA TACCCTACTA TGTAGGCCCC CTCGCCCGGG GAAATTCCAG
     5761 ATTCGCGTGG ATGACTCGCA AATCAGAAGA GACCATCACT CCCTGGAACT TCGAGGAAGT
     5821 CGTGGATAAG GGGGCCTCTG CCCAGTCCTT CATCGAAAGG ATGACTAACT TTGATAAAAA
     5881 TCTGCCTAAC GAAAAGGTGC TTCCTAAACA CTCTCTGCTG TACGAGTACT TCACAGTTTA
     5941 TAACGAGCTC ACCAAGGTCA AATACGTCAC AGAAGGGATG AGAAAGCCAG CATTCCTGTC
     6001 TGGAGAGCAG AAGAAAGCTA TCGTGGACCT CCTCTTCAAG ACGAACCGGA AAGTTACCGT
     6061 GAAACAGCTC AAAGAAGACT ATTTCAAAAA GATTGAATGT TTCGACTCTG TTGAAATCAG
     6121 CGGAGTGGAG GATCGCTTCA ACGCATCCCT GGGAACGTAT CACGATCTCC TGAAAATCAT
     6181 TAAAGACAAG GACTTCCTGG ACAATGAGGA GAACGAGGAC ATTCTTGAGG ACATTGTCCT
     6241 CACCCTTACG TTGTTTGAAG ATAGGGAGAT GATTGAAGAA CGCTTGAAAA CTTACGCTCA
     6301 TCTCTTCGAC GACAAAGTCA TGAAACAGCT CAAGAGGCGC CGATATACAG GATGGGGGCG
     6361 GCTGTCAAGA AAACTGATCA ATGGGATCCG AGACAAGCAG AGTGGAAAGA CAATCCTGGA
     6421 TTTTCTTAAG TCCGATGGAT TTGCCAACCG GAACTTCATG CAGTTGATCC ATGATGACTC
     6481 TCTCACCTTT AAGGAGGACA TCCAGAAAGC ACAAGTTTCT GGCCAGGGGG ACAGTCTTCA
     6541 CGAGCACATC GCTAATCTTG CAGGTAGCCC AGCTATCAAA AAGGGAATAC TGCAGACCGT
     6601 TAAGGTCGTG GATGAACTCG TCAAAGTAAT GGGAAGGCAT AAGCCCGAGA ATATCGTTAT
     6661 CGAGATGGCC CGAGAGAACC AAACTACCCA GAAGGGACAG AAGAACAGTA GGGAAAGGAT
     6721 GAAGAGGATT GAAGAGGGTA TAAAAGAACT GGGGTCCCAA ATCCTTAAGG AACACCCAGT
     6781 TGAAAACACC CAGCTTCAGA ATGAGAAGCT CTACCTGTAC TACCTGCAGA ACGGCAGGGA
     6841 CATGTACGTG GATCAGGAAC TGGACATCAA TCGGCTCTCC GACTACGACG TGGATCATAT
     6901 CGTGCCCCAG TCTTTTCTCA AAGATGATTC TATTGATAAT AAAGTGTTGA CAAGATCCGA
     6961 TAAAAATAGA GGGAAGAGTG ATAACGTCCC CTCAGAAGAA GTTGTCAAGA AAATGAAAAA
     7021 TTATTGGCGG CAGCTGCTGA ACGCCAAACT GATCACACAA CGGAAGTTCG ATAATCTGAC
     7081 TAAGGCTGAA CGAGGTGGCC TGTCTGAGTT GGATAAAGCC GGCTTCATCA AAAGGCAGCT
     7141 TGTTGAGACA CGCCAGATCA CCAAGCACGT GGCCCAAATT CTCGATTCAC GCATGAACAC
     7201 CAAGTACGAT GAAAATGACA AACTGATTCG AGAGGTGAAA GTTATTACTC TGAAGTCTAA
     7261 GCTGGTCTCA GATTTCAGAA AGGACTTTCA GTTTTATAAG GTGAGAGAGA TCAACAATTA
     7321 CCACCATGCG CATGATGCCT ACCTGAATGC AGTGGTAGGC ACTGCACTTA TCAAAAAATA
     7381 TCCCAAGCTT GAATCTGAAT TTGTTTACGG AGACTATAAA GTGTACGATG TTAGGAAAAT
     7441 GATCGCAAAG TCTGAGCAGG AAATAGGCAA GGCCACCGCT AAGTACTTCT TTTACAGCAA
     7501 TATTATGAAT TTTTTCAAGA CCGAGATTAC ACTGGCCAAT GGAGAGATTC GGAAGCGACC
     7561 ACTTATCGAA ACAAACGGAG AAACAGGAGA AATCGTGTGG GACAAGGGTA GGGATTTCGC
     7621 GACAGTCCGG AAGGTCCTGT CCATGCCGCA GGTGAACATC GTTAAAAAGA CCGAAGTACA
     7681 GACCGGAGGC TTCTCCAAGG AAAGTATCCT CCCGAAAAGG AACAGCGACA AGCTGATCGC
     7741 ACGCAAAAAA GATTGGGACC CCAAGAAATA CGGCGGATTC GATTCTCCTA CAGTCGCTTA
     7801 CAGTGTACTG GTTGTGGCCA AAGTGGAGAA AGGGAAGTCT AAAAAACTCA AAAGCGTCAA
     7861 GGAACTGCTG GGCATCACAA TCATGGAGCG ATCAAGCTTC GAAAAAAACC CCATCGACTT
     7921 TCTCGAGGCG AAAGGATATA AAGAGGTCAA AAAAGACCTC ATCATTAAGC TTCCCAAGTA
     7981 CTCTCTCTTT GAGCTTGAAA ACGGCCGGAA ACGAATGCTC GCTAGTGCGG GCGAGCTGCA
     8041 GAAAGGTAAC GAGCTGGCAC TGCCCTCTAA ATACGTTAAT TTCTTGTATC TGGCCAGCCA
     8101 CTATGAAAAG CTCAAAGGGT CTCCCGAAGA TAATGAGCAG AAGCAGCTGT TCGTGGAACA
     8161 ACACAAACAC TACCTTGATG AGATCATCGA GCAAATAAGC GAATTCTCCA AAAGAGTGAT
     8221 CCTCGCCGAC GCTAACCTCG ATAAGGTGCT TTCTGCTTAC AATAAGCACA GGGATAAGCC
     8281 CATCAGGGAG CAGGCAGAAA ACATTATCCA CTTGTTTACT CTGACCAACT TGGGCGCGCC
     8341 TGCAGCCTTC AAGTACTTCG ACACCACCAT AGACAGAAAG CGGTACACCT CTACAAAGGA
     8401 GGTCCTGGAC GCCACACTGA TTCATCAGTC AATTACGGGG CTCTATGAAA CAAGAATCGA
     8461 CCTCTCTCAG CTCGGTGGAG ACAGCAGGGC TGACCCCAAG AAGAAGAGGA AGGTGTGATC
     8521 TCTTCTCGAG TCATGTAATT AGTTATGTCA CGCTTACATT CACGCCCTCC CCCCACATCC
     8581 GCTCTAACCG AAAAGGAAGG AGTTAGACAA CCTGAAGTCT AGGTCCCTAT TTATTTTTTT
     8641 ATAGTTATGT TAGTATTAAG AACGTTATTT ATATTTCAAA TTTTTCTTTT TTTTCTGTAC
     8701 AGACGCGTGT ACGCATGTAA CATTATACTG AAAACCTTGC TTGAGAAGGT TTTGGGACGC
     8761 TCGAAGGCTT TAATTTGcca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     8821 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     8881 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     8941 ccaacagttg cgcagcctga aTGGCGAATG GCGCGACGCG CCCTGTAGCG GCGCATTAAG
     9001 CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC
     9061 CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC
     9121 TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA
     9181 AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG
     9241 CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC
     9301 ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA
     9361 TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC
     9421 GTTTACAATT TCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC
     9481 AGGCAAGTGC ACAAACAATA CTTAAATAAA TACTACTCAG TAATAACCTA TTTCTTAGCA
     9541 TTTTTGACGA AATTTGCTAT TTTGTTAGAG TCTTTTACAC CATTTGTCTC CACACCTCCG
     9601 CTTACATCAA CACCAATAAC GCCATTTAAT CTAAGCGCAT CACCAACATT TTCTGGCGTC
     9661 AGTCCACCAG CTAACATAAA ATGTAAGCTT TCGGGGCTCT CTTGCCTTCC AACCCAGTCA
     9721 GAAATCGAGT TCCAATCCAA AAGTTCACCT GTCCCACCTG CTTCTGAATC AAACAAGGGA
     9781 ATAAACGAAT GAGGTTTCTG TGAAGCTGCA CTGAGTAGTA TGTTGCAGTC TTTTGGAAAT
     9841 ACGAGTCTTT TAATAACTGG CAAACCGAGG AACTCTTGGT ATTCTTGCCA CGACTCATCT
     9901 CCATGCAGTT GGACGATATC AATGCCGTAA TCATTGACCA GAGCCAAAAC ATCCTCCTTA
     9961 GGTTGATTAC GAAACACGCC AACCAAGTAT TTCGGAGTGC CTGAACTATT TTTATATGCT
    10021 TTTACAAGAC TTGAAATTTT CCTTGCAATA ACCGGGTCAA TTGTTCTCTT TCTATTGGGC
    10081 ACACATATAA TACCCAGCAA GTCAGCATCG GAATCTAGAG CACATTCTGC GGCCTCTGTG
    10141 CTCTGCAAGC CGCAAACTTT CACCAATGGA CCAGAACTAC CTGTGAAATT AATAACAGAC
    10201 ATACTCCAAG CTGCCTTTGT GTGCTTAATC ACGTATACTC ACGTGCTCAA TAGTCACCAA
    10261 TGCCCTCCCT CTTGGCCCTC TCCTTTTCTT TTTTCGACCG AATTAATTCT TAATCGGCAA
    10321 AAAAAGAAAA GCTCCGGATC AAGATTGTAC GTAAGGTGAC AAGCTATTTT TCAATAAAGA
    10381 ATATCTTCCA CTACTGCCAT CTGGCGTCAT AACTGCAAAG TACACATATA TTACGATGCT
    10441 GTCTATTAAA TGCTTCCTAT ATTATATATA TAGTAATGTC GTTTGGTGCA CTCTCAGTAC
    10501 AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC CCGCTGACGC
    10561 GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG
    10621 GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGAC GAAAGGGCCT
    10681 CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG
    10741 TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC
    10801 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG
    10861 GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG
    10921 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT
    10981 GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT
    11041 TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT
    11101 ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA
    11161 TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG
    11221 AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC
    11281 AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTTCAC AACATGGGGG ATCATGTAAC
    11341 TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC
    11401 CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC
    11461 TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT
    11521 TCTGCGCTCG GCCCTTCcgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
    11581 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
    11641 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
    11701 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat at
//