LOCUS       pNSU299_YDR344C        11752 bp ds-DNA     circular     21-JAN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1225..1306
                     /label="sgRNA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3951..4357
                     /label="TEF1 promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    4358..4380
                     /label="kozak rev"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    8503..8517
                     /label="NLS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      3150..3547
                     /label="tGPM1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        1759..2518
                     /label="pGPM1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_binding    3850..3872
                     /label="LacO"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      1313..1750
                     /label="RPR1t"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      8978..9433
                     /label="f1 ori"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     gene            10670..11730
                     /label="Ampicillin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      8540..8779
                     /label="CYC1_terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      73..755
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        793..1203
                     /label="pRPR1"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1120..1203
                     /label="RNAse P leader sequence"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     Binding Site    1205..1224
                     /label="HXT6 Guide RNA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     gene            complement(9530..10204)
                     /label="TRP1"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1121..1139
                     /label="TetO"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             8905..8963
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     gene            4381..8496
                     /label="SpCas9"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     gene            complement(10205..10485)
                     /label="TRP1 prom"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1120..1120
                     /label="TSS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    8497..8517
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             2526..3149
                     /label="TetR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          3915..3934
                     /label="T3"
                     /note="sequence: AATTAACCCTCACTAAAGGG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(8817..8834)
                     /label="M13F"
                     /note="sequence: TGTAAAACGACGGCCAGT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(10728..10747)
                     /label="VF2"
                     /note="sequence: TGCCACCTGACGTCTAAGAA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          3592..3611
                     /label="VR"
                     /note="sequence: ATTACCGCCTTTGAGTGAGC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          10623..10641
                     /label="pGexR"
                     /note="sequence: GAGCTGCATGTGTCAGAGG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(8834..8856)
                     /label="M13F43"
                     /note="sequence: AGGGTTTTCCCAGTCACGACGTT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          3858..3881
                     /label="M13R49"
                     /note="sequence: GAGCGGATAACAATTTCACACAGG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(1225..1264)
                     /label="67-3'gRNA-extender"
                     /note="sequence: gactagccttattttaacttgctatttctagctctaaaac"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          1011..1032
                     /label="RPR1-fwd"
                     /note="sequence: caggatatgttaacgacccaca"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(10669..10690)
                     /label="pQEfPR"
                     /note="sequence: GTATCACGAGGCCCTTTCGTCT"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          8838..8859
                     /label="pNSU299-3455-FWD"
                     /note="sequence: tcgtgactgggaaaaccctggc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(10565..10585)
                     /label="pexF"
                     /note="sequence: GGAGCAGACAAGCCCGTCAGG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(8908..8929)
                     /label="pNSU299-3546-REV"
                     /note="sequence: tgcgggcctcttcgctattacg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(1312..1336)
                     /label="RPR1-rev"
                     /note="sequence: gattaaattggaagttggatatggc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          3811..3836
                     /label="pexR"
                     /note="sequence: CAGGCTTTACACTTTATGCTTCCGGC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1165..1204
                     /label="68-5'gRNA-extender"
                     /note="sequence: tcgcggctgggaacgaaactctgggagctgcgattggcag"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          9351..9368
                     /label="pEGFP-C1-2800-R"
                     /note="sequence: GATTTCGGCCTATTGGTT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          3878..3895
                     /label="M13R"
                     /note="sequence: CAGGAAACAGCTATGACC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(8788..8807)
                     /label="T7"
                     /note="sequence: TAATACGACTCACTATAGGG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(8549..8567)
                     /label="CYC1Reverse"
                     /note="sequence: GCGTGAATGTAAGCGTGAC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
       61 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
      121 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
      181 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
      241 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
      301 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
      361 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
      421 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      481 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
      541 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      601 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      661 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
      721 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
      781 ggccttttgc tcgggggatc tgccaattga acataacatg gtagttacat atactagtaa
      841 tatggttcgg cacacattaa aagtataaaa actatctgaa ttacgaatta catatattgg
      901 tcataaaaat caatcaatca tcgtgtgttt tatatgtctc ttatctaagt ataagaatat
      961 ccatagttaa tattcactta cgctaccttt taacctgtaa tcattgtcaa caggatatgt
     1021 taacgaccca cattgataaa cgctagtatt tctttttcct cttcttattg gccggctgtc
     1081 tctatactcc cctatagtct gtttcttttc gtttcgattg tccctatcag tgatagagat
     1141 ggcgcacatg gtacgctgtg gtgctcgcgg ctgggaacga aactctggga gctgcgattg
     1201 gcagTGAACA TGTTGCAACA ACTGgtttta gagctagaaa tagcaagtta aaataaggct
     1261 agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttctcg agccatatcc
     1321 aacttccaat ttaatctttc ttttttaatt ttcacttatt tgcgatacag aaagaaaaaa
     1381 gcgatagtaa ctattgaatt ttgtttggat ttggttagat tagatatggt ttctctttat
     1441 atttacatgc taaaaatggg ctacaccaga gatacataat tagatatata tacgccagta
     1501 caccttatcg gcccaagcct tgtcccaagg cagcgttttg ttcttggaaa cgctgcccta
     1561 cacgttcgct atgcttcaag aacttttctg agcacttcat gatgcatgtt tgttccttat
     1621 tggttagctt tgatgttgtg aagtcattga cacagtctgt gaaacatctt tctaccagat
     1681 tagagtacaa acgcatgaaa tccttcattt gcttttgttc cactactttt tggaactctt
     1741 gttgttcttt CCTGCAGGta gtcgtgcaat gtatgacttt aagatttgtg agcaggaaga
     1801 aaagggagaa tcttctaacg ataaaccctt gaaaaactgg gtagactacg ctatgttgag
     1861 ttgctacgca ggctgcacaa ttacacgaga atgctcccgc ctaggattta aggctaaggg
     1921 acgtgcaatg cagacgacag atctaaatga ccgtgtcggt gaagtgttcg ccaaactttt
     1981 cggttaacac atgcagtgat gcacgcgcga tggtgctaag ttacatatat atatatatat
     2041 atatatatat atatatatag ccatagtgat gtctaagtaa cctttatggt atatttctta
     2101 atgtggaaag atactagcgc gcgcacccac acacaagctt cgtcttttct tgaagaaaag
     2161 aggaagctcg ctaaatggga ttccactttc cgttccctgc cagctgatgg aaaaaggtta
     2221 gtggaacgat gaagaataaa aagagagatc cactgaggtg aaatttcagc tgacagcgag
     2281 tttcatgatc gtgatgaaca atggtaacga gttgtggctg ttgccaggga gggtggttct
     2341 caacttttaa tgtatggcca aatcgctact tgggtttgtt atataacaaa gaagaaataa
     2401 tgaactgatt ctcttcctcc ttcttgtcct ttcttaattc tgttgtaatt accttccttt
     2461 gtaatttttt ttgtaattat tcttcttaat aatccaaaca aacacacata ttacaatata
     2521 ttaaaatgtc tagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg
     2581 aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc
     2641 ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga
     2701 tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt
     2761 tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag
     2821 tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct
     2881 ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc
     2941 attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg
     3001 aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc
     3061 accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa
     3121 aacaacttaa atgtgaaagt gggtcttaag tctgaagaat gaatgatttg atgatttctt
     3181 tttccctcca tttttcttac tgaatatatc aatgatatag acttgtatag tttattattt
     3241 caaattaagt agctatatat agtcaagata acgtttgttt gacacgatta cattattcgt
     3301 cgacatcttt tttcagcctg tcgtggtagc aatttgagga gtattattaa ttgaataggt
     3361 tcattttgcg ctcgcataaa cagttttcgt cagggacagt atgttggaat gagtggtaat
     3421 taatggtgac atgacatgtt atagcaataa ccttgatgtt tacatcgtag tttaatgtac
     3481 accccgcgaa ttcgttcaag taggagtgca ccaattgcaa agggaaaagc tgaatgggca
     3541 gttcgaaCCG CGGtctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3601 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3661 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     3721 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     3781 aatgtgagtt acctcactca ttaggcaccc caggctttac actttatgct tccggctcct
     3841 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     3901 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggAGCT CATAGCTTCA
     3961 AAATGTTTCT ACTCCTTTTT TACTCTTCCA GATTTTCTCG GACTCCGCGC ATCGCCGTAC
     4021 CACTTCAAAA CACCCAAGCA CAGCATACTA AATTTCCCCT CTTTCTTCCT CTAGGGTGTC
     4081 GTTAATTACC CGTACTAAAG GTTTGGAAAA GAAAAAAGAG ACCGCCTCGT TTCTTTTTCT
     4141 TCGTCGAAAA AGGCAATAAA AATTTTTATC ACGTTTCTTT TTCTTGAAAA TTTTTTTTTT
     4201 GATTTTTTTC TCTTTCGATG ACCTCCCATT GATATTTAAG TTAATAAACG GTCTTCAATT
     4261 TCTCAAGTTT CAGTTTCATT TTTCTTGTTC TATTACAACT TTTTTTACTT CTTGCTCATT
     4321 AGAAAGAAAG CATAGCAATC TAATCTAAGT TTTCTAGAAC TAGTGGATCC CCCGGGaaaa
     4381 ATGGACAAGA AGTACTCCAT TGGGCTCGAT ATCGGCACAA ACAGCGTCGG tTGGGCCGTC
     4441 ATTACGGACG AGTACAAGGT GCCGAGCAAA AAATTCAAAG TTCTGGGCAA TACCGATCGC
     4501 CACAGCATAA AGAAGAACCT CATTGGCGCC CTCCTGTTCG ACTCCGGGGA GACGGCCGAA
     4561 GCCACGCGGC TCAAAAGAAC AGCACGGCGC AGATATACCC GCAGAAAGAA TCGGATCTGC
     4621 TACCTGCAGG AGATCTTTAG TAATGAGATG GCTAAGGTGG ATGACTCTTT CTTCCATAGG
     4681 CTGGAGGAGT CCTTTTTGGT GGAGGAGGAT AAAAAGCACG AGCGCCACCC AATCTTTGGC
     4741 AATATCGTGG ACGAGGTGGC GTACCATGAA AAGTACCCAA CCATATATCA TCTGAGGAAG
     4801 AAGCTTGTAG ACAGTACTGA TAAGGCTGAC TTGCGGTTGA TCTATCTCGC GCTGGCGCAT
     4861 ATGATCAAAT TTCGGGGACA CTTCCTCATC GAGGGGGACC TGAACCCAGA CAACAGCGAT
     4921 GTCGACAAAC TCTTTATCCA ACTGGTTCAG ACTTACAATC AGCTTTTCGA AGAGAACCCG
     4981 ATCAACGCAT CCGGAGTTGA CGCCAAAGCA ATCCTGAGCG CTAGGCTGTC CAAATCCCGG
     5041 CGGCTCGAAA ACCTCATCGC ACAGCTCCCT GGGGAGAAGA AGAACGGCCT GTTTGGTAAT
     5101 CTTATCGCCC TGTCACTCGG GCTGACCCCC AACTTTAAAT CTAACTTCGA CCTGGCCGAA
     5161 GATGCCAAGC TTCAACTGAG CAAAGACACC TACGATGATG ATCTCGACAA TCTGCTGGCC
     5221 CAGATCGGCG ACCAGTACGC AGACCTTTTT TTGGCGGCAA AGAACCTGTC AGACGCCATT
     5281 CTGCTGAGTG ATATTCTGCG AGTGAACACG GAGATCACCA AAGCTCCGCT GAGCGCTAGT
     5341 ATGATCAAGC GCTATGATGA GCACCACCAA GACTTGACTT TGCTGAAGGC CCTTGTCAGA
     5401 CAGCAACTGC CTGAGAAGTA CAAGGAAATT TTCTTCGATC AGTCTAAAAA TGGCTACGCC
     5461 GGATACATTG ACGGCGGAGC AAGCCAGGAG GAATTTTACA AATTTATTAA GCCCATCTTG
     5521 GAAAAAATGG ACGGCACCGA GGAGCTGCTG GTAAAGCTTA ACAGAGAAGA TCTGTTGCGC
     5581 AAACAGCGCA CTTTCGACAA TGGAAGCATC CCCCACCAGA TTCACCTGGG CGAACTGCAC
     5641 GCTATCCTCA GGCGGCAAGA GGATTTCTAC CCCTTTTTGA AAGATAACAG GGAAAAGATT
     5701 GAGAAAATCC TCACATTTCG GATACCCTAC TATGTAGGCC CCCTCGCCCG GGGAAATTCC
     5761 AGATTCGCGT GGATGACTCG CAAATCAGAA GAGACCATCA CTCCCTGGAA CTTCGAGGAA
     5821 GTCGTGGATA AGGGGGCCTC TGCCCAGTCC TTCATCGAAA GGATGACTAA CTTTGATAAA
     5881 AATCTGCCTA ACGAAAAGGT GCTTCCTAAA CACTCTCTGC TGTACGAGTA CTTCACAGTT
     5941 TATAACGAGC TCACCAAGGT CAAATACGTC ACAGAAGGGA TGAGAAAGCC AGCATTCCTG
     6001 TCTGGAGAGC AGAAGAAAGC TATCGTGGAC CTCCTCTTCA AGACGAACCG GAAAGTTACC
     6061 GTGAAACAGC TCAAAGAAGA CTATTTCAAA AAGATTGAAT GTTTCGACTC TGTTGAAATC
     6121 AGCGGAGTGG AGGATCGCTT CAACGCATCC CTGGGAACGT ATCACGATCT CCTGAAAATC
     6181 ATTAAAGACA AGGACTTCCT GGACAATGAG GAGAACGAGG ACATTCTTGA GGACATTGTC
     6241 CTCACCCTTA CGTTGTTTGA AGATAGGGAG ATGATTGAAG AACGCTTGAA AACTTACGCT
     6301 CATCTCTTCG ACGACAAAGT CATGAAACAG CTCAAGAGGC GCCGATATAC AGGATGGGGG
     6361 CGGCTGTCAA GAAAACTGAT CAATGGGATC CGAGACAAGC AGAGTGGAAA GACAATCCTG
     6421 GATTTTCTTA AGTCCGATGG ATTTGCCAAC CGGAACTTCA TGCAGTTGAT CCATGATGAC
     6481 TCTCTCACCT TTAAGGAGGA CATCCAGAAA GCACAAGTTT CTGGCCAGGG GGACAGTCTT
     6541 CACGAGCACA TCGCTAATCT TGCAGGTAGC CCAGCTATCA AAAAGGGAAT ACTGCAGACC
     6601 GTTAAGGTCG TGGATGAACT CGTCAAAGTA ATGGGAAGGC ATAAGCCCGA GAATATCGTT
     6661 ATCGAGATGG CCCGAGAGAA CCAAACTACC CAGAAGGGAC AGAAGAACAG TAGGGAAAGG
     6721 ATGAAGAGGA TTGAAGAGGG TATAAAAGAA CTGGGGTCCC AAATCCTTAA GGAACACCCA
     6781 GTTGAAAACA CCCAGCTTCA GAATGAGAAG CTCTACCTGT ACTACCTGCA GAACGGCAGG
     6841 GACATGTACG TGGATCAGGA ACTGGACATC AATCGGCTCT CCGACTACGA CGTGGATCAT
     6901 ATCGTGCCCC AGTCTTTTCT CAAAGATGAT TCTATTGATA ATAAAGTGTT GACAAGATCC
     6961 GATAAAAATA GAGGGAAGAG TGATAACGTC CCCTCAGAAG AAGTTGTCAA GAAAATGAAA
     7021 AATTATTGGC GGCAGCTGCT GAACGCCAAA CTGATCACAC AACGGAAGTT CGATAATCTG
     7081 ACTAAGGCTG AACGAGGTGG CCTGTCTGAG TTGGATAAAG CCGGCTTCAT CAAAAGGCAG
     7141 CTTGTTGAGA CACGCCAGAT CACCAAGCAC GTGGCCCAAA TTCTCGATTC ACGCATGAAC
     7201 ACCAAGTACG ATGAAAATGA CAAACTGATT CGAGAGGTGA AAGTTATTAC TCTGAAGTCT
     7261 AAGCTGGTCT CAGATTTCAG AAAGGACTTT CAGTTTTATA AGGTGAGAGA GATCAACAAT
     7321 TACCACCATG CGCATGATGC CTACCTGAAT GCAGTGGTAG GCACTGCACT TATCAAAAAA
     7381 TATCCCAAGC TTGAATCTGA ATTTGTTTAC GGAGACTATA AAGTGTACGA TGTTAGGAAA
     7441 ATGATCGCAA AGTCTGAGCA GGAAATAGGC AAGGCCACCG CTAAGTACTT CTTTTACAGC
     7501 AATATTATGA ATTTTTTCAA GACCGAGATT ACACTGGCCA ATGGAGAGAT TCGGAAGCGA
     7561 CCACTTATCG AAACAAACGG AGAAACAGGA GAAATCGTGT GGGACAAGGG TAGGGATTTC
     7621 GCGACAGTCC GGAAGGTCCT GTCCATGCCG CAGGTGAACA TCGTTAAAAA GACCGAAGTA
     7681 CAGACCGGAG GCTTCTCCAA GGAAAGTATC CTCCCGAAAA GGAACAGCGA CAAGCTGATC
     7741 GCACGCAAAA AAGATTGGGA CCCCAAGAAA TACGGCGGAT TCGATTCTCC TACAGTCGCT
     7801 TACAGTGTAC TGGTTGTGGC CAAAGTGGAG AAAGGGAAGT CTAAAAAACT CAAAAGCGTC
     7861 AAGGAACTGC TGGGCATCAC AATCATGGAG CGATCAAGCT TCGAAAAAAA CCCCATCGAC
     7921 TTTCTCGAGG CGAAAGGATA TAAAGAGGTC AAAAAAGACC TCATCATTAA GCTTCCCAAG
     7981 TACTCTCTCT TTGAGCTTGA AAACGGCCGG AAACGAATGC TCGCTAGTGC GGGCGAGCTG
     8041 CAGAAAGGTA ACGAGCTGGC ACTGCCCTCT AAATACGTTA ATTTCTTGTA TCTGGCCAGC
     8101 CACTATGAAA AGCTCAAAGG GTCTCCCGAA GATAATGAGC AGAAGCAGCT GTTCGTGGAA
     8161 CAACACAAAC ACTACCTTGA TGAGATCATC GAGCAAATAA GCGAATTCTC CAAAAGAGTG
     8221 ATCCTCGCCG ACGCTAACCT CGATAAGGTG CTTTCTGCTT ACAATAAGCA CAGGGATAAG
     8281 CCCATCAGGG AGCAGGCAGA AAACATTATC CACTTGTTTA CTCTGACCAA CTTGGGCGCG
     8341 CCTGCAGCCT TCAAGTACTT CGACACCACC ATAGACAGAA AGCGGTACAC CTCTACAAAG
     8401 GAGGTCCTGG ACGCCACACT GATTCATCAG TCAATTACGG GGCTCTATGA AACAAGAATC
     8461 GACCTCTCTC AGCTCGGTGG AGACAGCAGG GCTGACCCCA AGAAGAAGAG GAAGGTGTGA
     8521 TCTCTTCTCG AGTCATGTAA TTAGTTATGT CACGCTTACA TTCACGCCCT CCCCCCACAT
     8581 CCGCTCTAAC CGAAAAGGAA GGAGTTAGAC AACCTGAAGT CTAGGTCCCT ATTTATTTTT
     8641 TTATAGTTAT GTTAGTATTA AGAACGTTAT TTATATTTCA AATTTTTCTT TTTTTTCTGT
     8701 ACAGACGCGT GTACGCATGT AACATTATAC TGAAAACCTT GCTTGAGAAG GTTTTGGGAC
     8761 GCTCGAAGGC TTTAATTTGc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     8821 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     8881 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     8941 tcccaacagt tgcgcagcct gaaTGGCGAA TGGCGCGACG CGCCCTGTAG CGGCGCATTA
     9001 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG
     9061 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA
     9121 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC
     9181 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT
     9241 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA
     9301 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC
     9361 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA
     9421 ACGTTTACAA TTTCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC
     9481 GCAGGCAAGT GCACAAACAA TACTTAAATA AATACTACTC AGTAATAACC TATTTCTTAG
     9541 CATTTTTGAC GAAATTTGCT ATTTTGTTAG AGTCTTTTAC ACCATTTGTC TCCACACCTC
     9601 CGCTTACATC AACACCAATA ACGCCATTTA ATCTAAGCGC ATCACCAACA TTTTCTGGCG
     9661 TCAGTCCACC AGCTAACATA AAATGTAAGC TTTCGGGGCT CTCTTGCCTT CCAACCCAGT
     9721 CAGAAATCGA GTTCCAATCC AAAAGTTCAC CTGTCCCACC TGCTTCTGAA TCAAACAAGG
     9781 GAATAAACGA ATGAGGTTTC TGTGAAGCTG CACTGAGTAG TATGTTGCAG TCTTTTGGAA
     9841 ATACGAGTCT TTTAATAACT GGCAAACCGA GGAACTCTTG GTATTCTTGC CACGACTCAT
     9901 CTCCATGCAG TTGGACGATA TCAATGCCGT AATCATTGAC CAGAGCCAAA ACATCCTCCT
     9961 TAGGTTGATT ACGAAACACG CCAACCAAGT ATTTCGGAGT GCCTGAACTA TTTTTATATG
    10021 CTTTTACAAG ACTTGAAATT TTCCTTGCAA TAACCGGGTC AATTGTTCTC TTTCTATTGG
    10081 GCACACATAT AATACCCAGC AAGTCAGCAT CGGAATCTAG AGCACATTCT GCGGCCTCTG
    10141 TGCTCTGCAA GCCGCAAACT TTCACCAATG GACCAGAACT ACCTGTGAAA TTAATAACAG
    10201 ACATACTCCA AGCTGCCTTT GTGTGCTTAA TCACGTATAC TCACGTGCTC AATAGTCACC
    10261 AATGCCCTCC CTCTTGGCCC TCTCCTTTTC TTTTTTCGAC CGAATTAATT CTTAATCGGC
    10321 AAAAAAAGAA AAGCTCCGGA TCAAGATTGT ACGTAAGGTG ACAAGCTATT TTTCAATAAA
    10381 GAATATCTTC CACTACTGCC ATCTGGCGTC ATAACTGCAA AGTACACATA TATTACGATG
    10441 CTGTCTATTA AATGCTTCCT ATATTATATA TATAGTAATG TCGTTTGGTG CACTCTCAGT
    10501 ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC
    10561 GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC
    10621 GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC
    10681 CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA
    10741 GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT
    10801 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA
    10861 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT
    10921 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG
    10981 TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT
    11041 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG
    11101 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG
    11161 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA
    11221 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG
    11281 ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTTC ACAACATGGG GGATCATGTA
    11341 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC
    11401 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT
    11461 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA
    11521 CTTCTGCGCT CGGCCCTTCc ggctggctgg tttattgctg ataaatctgg agccggtgag
    11581 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
    11641 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
    11701 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc at
//