LOCUS       pNSU299-APL3           11752 bp ds-DNA     circular     21-JAN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    678..692
                     /label="NLS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      5240..5677
                     /label="RPR1t"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        4720..5130
                     /label="pRPR1"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    7878..8284
                     /label="TEF1 promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      1153..1608
                     /label="f1 ori"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             6453..7076
                     /label="TetR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5048..5066
                     /label="TetO"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    5047..5130
                     /label="RNAse P leader sequence"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    672..692
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             1080..1138
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    5047..5047
                     /label="TSS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      4000..4682
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      7077..7474
                     /label="tGPM1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      715..954
                     /label="CYC1_terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     gene            complement(1705..2379)
                     /label="TRP1"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     gene            2845..3905
                     /label="Ampicillin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        5686..6445
                     /label="pGPM1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     gene            complement(2380..2660)
                     /label="TRP1 prom"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    8285..8307
                     /label="kozak rev"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5152..5233
                     /label="sgRNA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_binding    7777..7799
                     /label="LacO"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     gene            join(8308..11752,1..671)
                     /label="SpCas9"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          1013..1034
                     /label="pNSU299-3455-FWD"
                     /note="sequence: tcgtgactgggaaaaccctggc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          7519..7538
                     /label="VR"
                     /note="sequence: ATTACCGCCTTTGAGTGAGC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1526..1543
                     /label="pEGFP-C1-2800-R"
                     /note="sequence: GATTTCGGCCTATTGGTT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(1083..1104)
                     /label="pNSU299-3546-REV"
                     /note="sequence: tgcgggcctcttcgctattacg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(963..982)
                     /label="T7"
                     /note="sequence: TAATACGACTCACTATAGGG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          7842..7861
                     /label="T3"
                     /note="sequence: AATTAACCCTCACTAAAGGG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(2740..2760)
                     /label="pexF"
                     /note="sequence: GGAGCAGACAAGCCCGTCAGG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          7805..7822
                     /label="M13R"
                     /note="sequence: CAGGAAACAGCTATGACC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(1009..1031)
                     /label="M13F43"
                     /note="sequence: AGGGTTTTCCCAGTCACGACGTT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(2844..2865)
                     /label="pQEfPR"
                     /note="sequence: GTATCACGAGGCCCTTTCGTCT"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          7738..7763
                     /label="pexR"
                     /note="sequence: CAGGCTTTACACTTTATGCTTCCGGC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          2798..2816
                     /label="pGexR"
                     /note="sequence: GAGCTGCATGTGTCAGAGG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(724..742)
                     /label="CYC1Reverse"
                     /note="sequence: GCGTGAATGTAAGCGTGAC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(5152..5191)
                     /label="67-3'gRNA-extender"
                     /note="sequence: gactagccttattttaacttgctatttctagctctaaaac"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(5239..5263)
                     /label="RPR1-rev"
                     /note="sequence: gattaaattggaagttggatatggc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          4938..4959
                     /label="RPR1-fwd"
                     /note="sequence: caggatatgttaacgacccaca"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          7785..7808
                     /label="M13R49"
                     /note="sequence: GAGCGGATAACAATTTCACACAGG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(992..1009)
                     /label="M13F"
                     /note="sequence: TGTAAAACGACGGCCAGT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(2903..2922)
                     /label="VF2"
                     /note="sequence: TGCCACCTGACGTCTAAGAA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 TGGAGAAAGG GAAGTCTAAA AAACTCAAAA GCGTCAAGGA ACTGCTGGGC ATCACAATCA
       61 TGGAGCGATC AAGCTTCGAA AAAAACCCCA TCGACTTTCT CGAGGCGAAA GGATATAAAG
      121 AGGTCAAAAA AGACCTCATC ATTAAGCTTC CCAAGTACTC TCTCTTTGAG CTTGAAAACG
      181 GCCGGAAACG AATGCTCGCT AGTGCGGGCG AGCTGCAGAA AGGTAACGAG CTGGCACTGC
      241 CCTCTAAATA CGTTAATTTC TTGTATCTGG CCAGCCACTA TGAAAAGCTC AAAGGGTCTC
      301 CCGAAGATAA TGAGCAGAAG CAGCTGTTCG TGGAACAACA CAAACACTAC CTTGATGAGA
      361 TCATCGAGCA AATAAGCGAA TTCTCCAAAA GAGTGATCCT CGCCGACGCT AACCTCGATA
      421 AGGTGCTTTC TGCTTACAAT AAGCACAGGG ATAAGCCCAT CAGGGAGCAG GCAGAAAACA
      481 TTATCCACTT GTTTACTCTG ACCAACTTGG GCGCGCCTGC AGCCTTCAAG TACTTCGACA
      541 CCACCATAGA CAGAAAGCGG TACACCTCTA CAAAGGAGGT CCTGGACGCC ACACTGATTC
      601 ATCAGTCAAT TACGGGGCTC TATGAAACAA GAATCGACCT CTCTCAGCTC GGTGGAGACA
      661 GCAGGGCTGA CCCCAAGAAG AAGAGGAAGG TGTGATCTCT TCTCGAGTCA TGTAATTAGT
      721 TATGTCACGC TTACATTCAC GCCCTCCCCC CACATCCGCT CTAACCGAAA AGGAAGGAGT
      781 TAGACAACCT GAAGTCTAGG TCCCTATTTA TTTTTTTATA GTTATGTTAG TATTAAGAAC
      841 GTTATTTATA TTTCAAATTT TTCTTTTTTT TCTGTACAGA CGCGTGTACG CATGTAACAT
      901 TATACTGAAA ACCTTGCTTG AGAAGGTTTT GGGACGCTCG AAGGCTTTAA TTTGccaatt
      961 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     1021 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     1081 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaaTG
     1141 GCGAATGGCG CGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC
     1201 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT
     1261 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG
     1321 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT
     1381 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT
     1441 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT
     1501 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT
     1561 AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGTT TACAATTTCC TGATGCGGTA
     1621 TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCAGG CAAGTGCACA AACAATACTT
     1681 AAATAAATAC TACTCAGTAA TAACCTATTT CTTAGCATTT TTGACGAAAT TTGCTATTTT
     1741 GTTAGAGTCT TTTACACCAT TTGTCTCCAC ACCTCCGCTT ACATCAACAC CAATAACGCC
     1801 ATTTAATCTA AGCGCATCAC CAACATTTTC TGGCGTCAGT CCACCAGCTA ACATAAAATG
     1861 TAAGCTTTCG GGGCTCTCTT GCCTTCCAAC CCAGTCAGAA ATCGAGTTCC AATCCAAAAG
     1921 TTCACCTGTC CCACCTGCTT CTGAATCAAA CAAGGGAATA AACGAATGAG GTTTCTGTGA
     1981 AGCTGCACTG AGTAGTATGT TGCAGTCTTT TGGAAATACG AGTCTTTTAA TAACTGGCAA
     2041 ACCGAGGAAC TCTTGGTATT CTTGCCACGA CTCATCTCCA TGCAGTTGGA CGATATCAAT
     2101 GCCGTAATCA TTGACCAGAG CCAAAACATC CTCCTTAGGT TGATTACGAA ACACGCCAAC
     2161 CAAGTATTTC GGAGTGCCTG AACTATTTTT ATATGCTTTT ACAAGACTTG AAATTTTCCT
     2221 TGCAATAACC GGGTCAATTG TTCTCTTTCT ATTGGGCACA CATATAATAC CCAGCAAGTC
     2281 AGCATCGGAA TCTAGAGCAC ATTCTGCGGC CTCTGTGCTC TGCAAGCCGC AAACTTTCAC
     2341 CAATGGACCA GAACTACCTG TGAAATTAAT AACAGACATA CTCCAAGCTG CCTTTGTGTG
     2401 CTTAATCACG TATACTCACG TGCTCAATAG TCACCAATGC CCTCCCTCTT GGCCCTCTCC
     2461 TTTTCTTTTT TCGACCGAAT TAATTCTTAA TCGGCAAAAA AAGAAAAGCT CCGGATCAAG
     2521 ATTGTACGTA AGGTGACAAG CTATTTTTCA ATAAAGAATA TCTTCCACTA CTGCCATCTG
     2581 GCGTCATAAC TGCAAAGTAC ACATATATTA CGATGCTGTC TATTAAATGC TTCCTATATT
     2641 ATATATATAG TAATGTCGTT TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT
     2701 TAAGCCAGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC
     2761 CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT
     2821 CACCGTCATC ACCGAAACGC GCGAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG
     2881 TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC
     2941 GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC
     3001 AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT
     3061 TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG
     3121 AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG
     3181 AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA
     3241 TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC
     3301 AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG
     3361 TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA
     3421 CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC
     3481 TAACCGCTTT TTTTCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG
     3541 AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA
     3601 CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA
     3661 TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCcggctg
     3721 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3781 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     3841 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3901 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3961 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4021 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4081 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4141 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4201 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     4261 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4321 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4381 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4441 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4501 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4561 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4621 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4681 cctttttacg gttcctggcc ttttgctggc cttttgctcg ggggatctgc caattgaaca
     4741 taacatggta gttacatata ctagtaatat ggttcggcac acattaaaag tataaaaact
     4801 atctgaatta cgaattacat atattggtca taaaaatcaa tcaatcatcg tgtgttttat
     4861 atgtctctta tctaagtata agaatatcca tagttaatat tcacttacgc taccttttaa
     4921 cctgtaatca ttgtcaacag gatatgttaa cgacccacat tgataaacgc tagtatttct
     4981 ttttcctctt cttattggcc ggctgtctct atactcccct atagtctgtt tcttttcgtt
     5041 tcgattgtcc ctatcagtga tagagatggc gcacatggta cgctgtggtg ctcgcggctg
     5101 ggaacgaaac tctgggagct gcgattggca gGATCCTCGC TCCAAATTGG Agttttagag
     5161 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     5221 tcggtgcttt tttctcgagc catatccaac ttccaattta atctttcttt tttaattttc
     5281 acttatttgc gatacagaaa gaaaaaagcg atagtaacta ttgaattttg tttggatttg
     5341 gttagattag atatggtttc tctttatatt tacatgctaa aaatgggcta caccagagat
     5401 acataattag atatatatac gccagtacac cttatcggcc caagccttgt cccaaggcag
     5461 cgttttgttc ttggaaacgc tgccctacac gttcgctatg cttcaagaac ttttctgagc
     5521 acttcatgat gcatgtttgt tccttattgg ttagctttga tgttgtgaag tcattgacac
     5581 agtctgtgaa acatctttct accagattag agtacaaacg catgaaatcc ttcatttgct
     5641 tttgttccac tactttttgg aactcttgtt gttctttCCT GCAGGtagtc gtgcaatgta
     5701 tgactttaag atttgtgagc aggaagaaaa gggagaatct tctaacgata aacccttgaa
     5761 aaactgggta gactacgcta tgttgagttg ctacgcaggc tgcacaatta cacgagaatg
     5821 ctcccgccta ggatttaagg ctaagggacg tgcaatgcag acgacagatc taaatgaccg
     5881 tgtcggtgaa gtgttcgcca aacttttcgg ttaacacatg cagtgatgca cgcgcgatgg
     5941 tgctaagtta catatatata tatatatata tatatatata tatatagcca tagtgatgtc
     6001 taagtaacct ttatggtata tttcttaatg tggaaagata ctagcgcgcg cacccacaca
     6061 caagcttcgt cttttcttga agaaaagagg aagctcgcta aatgggattc cactttccgt
     6121 tccctgccag ctgatggaaa aaggttagtg gaacgatgaa gaataaaaag agagatccac
     6181 tgaggtgaaa tttcagctga cagcgagttt catgatcgtg atgaacaatg gtaacgagtt
     6241 gtggctgttg ccagggaggg tggttctcaa cttttaatgt atggccaaat cgctacttgg
     6301 gtttgttata taacaaagaa gaaataatga actgattctc ttcctccttc ttgtcctttc
     6361 ttaattctgt tgtaattacc ttcctttgta attttttttg taattattct tcttaataat
     6421 ccaaacaaac acacatatta caatatatta aaatgtctag attagataaa agtaaagtga
     6481 ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac
     6541 tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg
     6601 ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt
     6661 tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt
     6721 tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt
     6781 atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg
     6841 cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag
     6901 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat
     6961 tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc
     7021 ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tcttaagtct
     7081 gaagaatgaa tgatttgatg atttcttttt ccctccattt ttcttactga atatatcaat
     7141 gatatagact tgtatagttt attatttcaa attaagtagc tatatatagt caagataacg
     7201 tttgtttgac acgattacat tattcgtcga catctttttt cagcctgtcg tggtagcaat
     7261 ttgaggagta ttattaattg aataggttca ttttgcgctc gcataaacag ttttcgtcag
     7321 ggacagtatg ttggaatgag tggtaattaa tggtgacatg acatgttata gcaataacct
     7381 tgatgtttac atcgtagttt aatgtacacc ccgcgaattc gttcaagtag gagtgcacca
     7441 attgcaaagg gaaaagctga atgggcagtt cgaaCCGCGG tctttcctgc gttatcccct
     7501 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7561 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7621 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7681 aaagcgggca gtgagcgcaa cgcaattaat gtgagttacc tcactcatta ggcaccccag
     7741 gctttacact ttatgcttcc ggctcctatg ttgtgtggaa ttgtgagcgg ataacaattt
     7801 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     7861 gaacaaaagc tggAGCTCAT AGCTTCAAAA TGTTTCTACT CCTTTTTTAC TCTTCCAGAT
     7921 TTTCTCGGAC TCCGCGCATC GCCGTACCAC TTCAAAACAC CCAAGCACAG CATACTAAAT
     7981 TTCCCCTCTT TCTTCCTCTA GGGTGTCGTT AATTACCCGT ACTAAAGGTT TGGAAAAGAA
     8041 AAAAGAGACC GCCTCGTTTC TTTTTCTTCG TCGAAAAAGG CAATAAAAAT TTTTATCACG
     8101 TTTCTTTTTC TTGAAAATTT TTTTTTTGAT TTTTTTCTCT TTCGATGACC TCCCATTGAT
     8161 ATTTAAGTTA ATAAACGGTC TTCAATTTCT CAAGTTTCAG TTTCATTTTT CTTGTTCTAT
     8221 TACAACTTTT TTTACTTCTT GCTCATTAGA AAGAAAGCAT AGCAATCTAA TCTAAGTTTT
     8281 CTAGAACTAG TGGATCCCCC GGGaaaaATG GACAAGAAGT ACTCCATTGG GCTCGATATC
     8341 GGCACAAACA GCGTCGGtTG GGCCGTCATT ACGGACGAGT ACAAGGTGCC GAGCAAAAAA
     8401 TTCAAAGTTC TGGGCAATAC CGATCGCCAC AGCATAAAGA AGAACCTCAT TGGCGCCCTC
     8461 CTGTTCGACT CCGGGGAGAC GGCCGAAGCC ACGCGGCTCA AAAGAACAGC ACGGCGCAGA
     8521 TATACCCGCA GAAAGAATCG GATCTGCTAC CTGCAGGAGA TCTTTAGTAA TGAGATGGCT
     8581 AAGGTGGATG ACTCTTTCTT CCATAGGCTG GAGGAGTCCT TTTTGGTGGA GGAGGATAAA
     8641 AAGCACGAGC GCCACCCAAT CTTTGGCAAT ATCGTGGACG AGGTGGCGTA CCATGAAAAG
     8701 TACCCAACCA TATATCATCT GAGGAAGAAG CTTGTAGACA GTACTGATAA GGCTGACTTG
     8761 CGGTTGATCT ATCTCGCGCT GGCGCATATG ATCAAATTTC GGGGACACTT CCTCATCGAG
     8821 GGGGACCTGA ACCCAGACAA CAGCGATGTC GACAAACTCT TTATCCAACT GGTTCAGACT
     8881 TACAATCAGC TTTTCGAAGA GAACCCGATC AACGCATCCG GAGTTGACGC CAAAGCAATC
     8941 CTGAGCGCTA GGCTGTCCAA ATCCCGGCGG CTCGAAAACC TCATCGCACA GCTCCCTGGG
     9001 GAGAAGAAGA ACGGCCTGTT TGGTAATCTT ATCGCCCTGT CACTCGGGCT GACCCCCAAC
     9061 TTTAAATCTA ACTTCGACCT GGCCGAAGAT GCCAAGCTTC AACTGAGCAA AGACACCTAC
     9121 GATGATGATC TCGACAATCT GCTGGCCCAG ATCGGCGACC AGTACGCAGA CCTTTTTTTG
     9181 GCGGCAAAGA ACCTGTCAGA CGCCATTCTG CTGAGTGATA TTCTGCGAGT GAACACGGAG
     9241 ATCACCAAAG CTCCGCTGAG CGCTAGTATG ATCAAGCGCT ATGATGAGCA CCACCAAGAC
     9301 TTGACTTTGC TGAAGGCCCT TGTCAGACAG CAACTGCCTG AGAAGTACAA GGAAATTTTC
     9361 TTCGATCAGT CTAAAAATGG CTACGCCGGA TACATTGACG GCGGAGCAAG CCAGGAGGAA
     9421 TTTTACAAAT TTATTAAGCC CATCTTGGAA AAAATGGACG GCACCGAGGA GCTGCTGGTA
     9481 AAGCTTAACA GAGAAGATCT GTTGCGCAAA CAGCGCACTT TCGACAATGG AAGCATCCCC
     9541 CACCAGATTC ACCTGGGCGA ACTGCACGCT ATCCTCAGGC GGCAAGAGGA TTTCTACCCC
     9601 TTTTTGAAAG ATAACAGGGA AAAGATTGAG AAAATCCTCA CATTTCGGAT ACCCTACTAT
     9661 GTAGGCCCCC TCGCCCGGGG AAATTCCAGA TTCGCGTGGA TGACTCGCAA ATCAGAAGAG
     9721 ACCATCACTC CCTGGAACTT CGAGGAAGTC GTGGATAAGG GGGCCTCTGC CCAGTCCTTC
     9781 ATCGAAAGGA TGACTAACTT TGATAAAAAT CTGCCTAACG AAAAGGTGCT TCCTAAACAC
     9841 TCTCTGCTGT ACGAGTACTT CACAGTTTAT AACGAGCTCA CCAAGGTCAA ATACGTCACA
     9901 GAAGGGATGA GAAAGCCAGC ATTCCTGTCT GGAGAGCAGA AGAAAGCTAT CGTGGACCTC
     9961 CTCTTCAAGA CGAACCGGAA AGTTACCGTG AAACAGCTCA AAGAAGACTA TTTCAAAAAG
    10021 ATTGAATGTT TCGACTCTGT TGAAATCAGC GGAGTGGAGG ATCGCTTCAA CGCATCCCTG
    10081 GGAACGTATC ACGATCTCCT GAAAATCATT AAAGACAAGG ACTTCCTGGA CAATGAGGAG
    10141 AACGAGGACA TTCTTGAGGA CATTGTCCTC ACCCTTACGT TGTTTGAAGA TAGGGAGATG
    10201 ATTGAAGAAC GCTTGAAAAC TTACGCTCAT CTCTTCGACG ACAAAGTCAT GAAACAGCTC
    10261 AAGAGGCGCC GATATACAGG ATGGGGGCGG CTGTCAAGAA AACTGATCAA TGGGATCCGA
    10321 GACAAGCAGA GTGGAAAGAC AATCCTGGAT TTTCTTAAGT CCGATGGATT TGCCAACCGG
    10381 AACTTCATGC AGTTGATCCA TGATGACTCT CTCACCTTTA AGGAGGACAT CCAGAAAGCA
    10441 CAAGTTTCTG GCCAGGGGGA CAGTCTTCAC GAGCACATCG CTAATCTTGC AGGTAGCCCA
    10501 GCTATCAAAA AGGGAATACT GCAGACCGTT AAGGTCGTGG ATGAACTCGT CAAAGTAATG
    10561 GGAAGGCATA AGCCCGAGAA TATCGTTATC GAGATGGCCC GAGAGAACCA AACTACCCAG
    10621 AAGGGACAGA AGAACAGTAG GGAAAGGATG AAGAGGATTG AAGAGGGTAT AAAAGAACTG
    10681 GGGTCCCAAA TCCTTAAGGA ACACCCAGTT GAAAACACCC AGCTTCAGAA TGAGAAGCTC
    10741 TACCTGTACT ACCTGCAGAA CGGCAGGGAC ATGTACGTGG ATCAGGAACT GGACATCAAT
    10801 CGGCTCTCCG ACTACGACGT GGATCATATC GTGCCCCAGT CTTTTCTCAA AGATGATTCT
    10861 ATTGATAATA AAGTGTTGAC AAGATCCGAT AAAAATAGAG GGAAGAGTGA TAACGTCCCC
    10921 TCAGAAGAAG TTGTCAAGAA AATGAAAAAT TATTGGCGGC AGCTGCTGAA CGCCAAACTG
    10981 ATCACACAAC GGAAGTTCGA TAATCTGACT AAGGCTGAAC GAGGTGGCCT GTCTGAGTTG
    11041 GATAAAGCCG GCTTCATCAA AAGGCAGCTT GTTGAGACAC GCCAGATCAC CAAGCACGTG
    11101 GCCCAAATTC TCGATTCACG CATGAACACC AAGTACGATG AAAATGACAA ACTGATTCGA
    11161 GAGGTGAAAG TTATTACTCT GAAGTCTAAG CTGGTCTCAG ATTTCAGAAA GGACTTTCAG
    11221 TTTTATAAGG TGAGAGAGAT CAACAATTAC CACCATGCGC ATGATGCCTA CCTGAATGCA
    11281 GTGGTAGGCA CTGCACTTAT CAAAAAATAT CCCAAGCTTG AATCTGAATT TGTTTACGGA
    11341 GACTATAAAG TGTACGATGT TAGGAAAATG ATCGCAAAGT CTGAGCAGGA AATAGGCAAG
    11401 GCCACCGCTA AGTACTTCTT TTACAGCAAT ATTATGAATT TTTTCAAGAC CGAGATTACA
    11461 CTGGCCAATG GAGAGATTCG GAAGCGACCA CTTATCGAAA CAAACGGAGA AACAGGAGAA
    11521 ATCGTGTGGG ACAAGGGTAG GGATTTCGCG ACAGTCCGGA AGGTCCTGTC CATGCCGCAG
    11581 GTGAACATCG TTAAAAAGAC CGAAGTACAG ACCGGAGGCT TCTCCAAGGA AAGTATCCTC
    11641 CCGAAAAGGA ACAGCGACAA GCTGATCGCA CGCAAAAAAG ATTGGGACCC CAAGAAATAC
    11701 GGCGGATTCG ATTCTCCTAC AGTCGCTTAC AGTGTACTGG TTGTGGCCAA AG
//