LOCUS       pNSU299_CPR1_1         11752 bp ds-DNA     circular     21-JAN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     gene            complement(1918..2198)
                     /label="TRP1 prom"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             5991..6614
                     /label="TetR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    216..230
                     /label="NLS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      6615..7012
                     /label="tGPM1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    210..230
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4585..4585
                     /label="TSS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        5224..5983
                     /label="pGPM1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    7823..7845
                     /label="kozak rev"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        4258..4669
                     /label="pRPR1"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     terminator      253..492
                     /label="CYC1_terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      3538..4220
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4585..4669
                     /label="RNAse P leader sequence"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_binding    7315..7337
                     /label="LacO"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    7416..7822
                     /label="TEF1 promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     gene            join(7846..11752,1..209)
                     /label="SpCas9"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             618..676
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     rep_origin      691..1146
                     /label="f1 ori"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4690..4771
                     /label="sgRNA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     gene            2383..3443
                     /label="Ampicillin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      4778..5215
                     /label="RPR1t"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     gene            complement(1243..1917)
                     /label="TRP1"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    4586..4604
                     /label="TetO"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          join(11747..11752,1..18)
                     /label="pNSU299-8304 F"
                     /note="sequence: CCATCAGGGAGCAGGCAGAAAACA"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(4690..4729)
                     /label="67-3'gRNA-extender"
                     /note="sequence: gactagccttattttaacttgctatttctagctctaaaac"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(530..547)
                     /label="M13F"
                     /note="sequence: TGTAAAACGACGGCCAGT"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(501..520)
                     /label="T7"
                     /note="sequence: TAATACGACTCACTATAGGG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(2278..2298)
                     /label="pexF"
                     /note="sequence: GGAGCAGACAAGCCCGTCAGG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          1064..1081
                     /label="pEGFP-C1-2800-R"
                     /note="sequence: GATTTCGGCCTATTGGTT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          4476..4497
                     /label="RPR1-fwd"
                     /note="sequence: caggatatgttaacgacccaca"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          7323..7346
                     /label="M13R49"
                     /note="sequence: GAGCGGATAACAATTTCACACAGG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          7850..7873
                     /label="Cas9-28 FWD"
                     /note="sequence: ACAAGAAGTACTCCATTGGGCTCG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          8515..8536
                     /label="Cas9-691 FWD"
                     /note="sequence: AACCTCATCGCACAGCTCCCTG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(262..280)
                     /label="CYC1Reverse"
                     /note="sequence: GCGTGAATGTAAGCGTGAC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          9348..9367
                     /label="Cas9-1522 FWD"
                     /note="sequence: TCTGCCTAACGAAAAGGTGC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2336..2354
                     /label="pGexR"
                     /note="sequence: GAGCTGCATGTGTCAGAGG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(2382..2403)
                     /label="pQEfPR"
                     /note="sequence: GTATCACGAGGCCCTTTCGTCT"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          4630..4669
                     /label="68-5'gRNA-extender"
                     /note="sequence: tcgcggctgggaacgaaactctgggagctgcgattggcag"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(547..569)
                     /label="M13F43"
                     /note="sequence: AGGGTTTTCCCAGTCACGACGTT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          7343..7360
                     /label="M13R"
                     /note="sequence: CAGGAAACAGCTATGACC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          551..572
                     /label="pNSU299-3455-FWD"
                     /note="sequence: tcgtgactgggaaaaccctggc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(7120..7141)
                     /label="pNSU299-3674-REV"
                     /note="sequence: gctcttccgcttcctcgctcac"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(4777..4801)
                     /label="RPR1-rev"
                     /note="sequence: gattaaattggaagttggatatggc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          10126..10147
                     /label="Cas9 -2302 FWD"
                     /note="sequence: ATCGAGATGGCCCGAGAGAACC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(530..547)
                     /label="M13F"
                     /note="sequence: TGTAAAACGACGGCCAGT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          10934..10953
                     /label="Cas9-3108 FWD"
                     /note="sequence: GCAAGGCCACCGCTAAGTAC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(2441..2460)
                     /label="VF2"
                     /note="sequence: TGCCACCTGACGTCTAAGAA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          7057..7076
                     /label="VR"
                     /note="sequence: ATTACCGCCTTTGAGTGAGC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          3961..3982
                     /label="pNSU299-516-F"
                     /note="sequence: tggagcgaacgacctacaccga"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          5859..5884
                     /label="497_GPM1_FwdSeq"
                     /note="sequence: gaaataatgaactgattctcttcctc"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(621..642)
                     /label="pNSU299-3546-REV"
                     /note="sequence: tgcgggcctcttcgctattacg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          7276..7301
                     /label="pexR"
                     /note="sequence: CAGGCTTTACACTTTATGCTTCCGGC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          5139..5162
                     /label="pNSU299-1697-F"
                     /note="sequence: accagattagagtacaaacgcatg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          7380..7399
                     /label="T3"
                     /note="sequence: AATTAACCCTCACTAAAGGG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 GGGAGCAGGC AGAAAACATT ATCCACTTGT TTACTCTGAC CAACTTGGGC GCGCCTGCAG
       61 CCTTCAAGTA CTTCGACACC ACCATAGACA GAAAGCGGTA CACCTCTACA AAGGAGGTCC
      121 TGGACGCCAC ACTGATTCAT CAGTCAATTA CGGGGCTCTA TGAAACAAGA ATCGACCTCT
      181 CTCAGCTCGG TGGAGACAGC AGGGCTGACC CCAAGAAGAA GAGGAAGGTG TGATCTCTTC
      241 TCGAGTCATG TAATTAGTTA TGTCACGCTT ACATTCACGC CCTCCCCCCA CATCCGCTCT
      301 AACCGAAAAG GAAGGAGTTA GACAACCTGA AGTCTAGGTC CCTATTTATT TTTTTATAGT
      361 TATGTTAGTA TTAAGAACGT TATTTATATT TCAAATTTTT CTTTTTTTTC TGTACAGACG
      421 CGTGTACGCA TGTAACATTA TACTGAAAAC CTTGCTTGAG AAGGTTTTGG GACGCTCGAA
      481 GGCTTTAATT TGccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
      541 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      601 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      661 agttgcgcag cctgaaTGGC GAATGGCGCG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG
      721 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC
      781 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA
      841 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC
      901 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT
      961 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA
     1021 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT
     1081 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA
     1141 CAATTTCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCAGGCA
     1201 AGTGCACAAA CAATACTTAA ATAAATACTA CTCAGTAATA ACCTATTTCT TAGCATTTTT
     1261 GACGAAATTT GCTATTTTGT TAGAGTCTTT TACACCATTT GTCTCCACAC CTCCGCTTAC
     1321 ATCAACACCA ATAACGCCAT TTAATCTAAG CGCATCACCA ACATTTTCTG GCGTCAGTCC
     1381 ACCAGCTAAC ATAAAATGTA AGCTTTCGGG GCTCTCTTGC CTTCCAACCC AGTCAGAAAT
     1441 CGAGTTCCAA TCCAAAAGTT CACCTGTCCC ACCTGCTTCT GAATCAAACA AGGGAATAAA
     1501 CGAATGAGGT TTCTGTGAAG CTGCACTGAG TAGTATGTTG CAGTCTTTTG GAAATACGAG
     1561 TCTTTTAATA ACTGGCAAAC CGAGGAACTC TTGGTATTCT TGCCACGACT CATCTCCATG
     1621 CAGTTGGACG ATATCAATGC CGTAATCATT GACCAGAGCC AAAACATCCT CCTTAGGTTG
     1681 ATTACGAAAC ACGCCAACCA AGTATTTCGG AGTGCCTGAA CTATTTTTAT ATGCTTTTAC
     1741 AAGACTTGAA ATTTTCCTTG CAATAACCGG GTCAATTGTT CTCTTTCTAT TGGGCACACA
     1801 TATAATACCC AGCAAGTCAG CATCGGAATC TAGAGCACAT TCTGCGGCCT CTGTGCTCTG
     1861 CAAGCCGCAA ACTTTCACCA ATGGACCAGA ACTACCTGTG AAATTAATAA CAGACATACT
     1921 CCAAGCTGCC TTTGTGTGCT TAATCACGTA TACTCACGTG CTCAATAGTC ACCAATGCCC
     1981 TCCCTCTTGG CCCTCTCCTT TTCTTTTTTC GACCGAATTA ATTCTTAATC GGCAAAAAAA
     2041 GAAAAGCTCC GGATCAAGAT TGTACGTAAG GTGACAAGCT ATTTTTCAAT AAAGAATATC
     2101 TTCCACTACT GCCATCTGGC GTCATAACTG CAAAGTACAC ATATATTACG ATGCTGTCTA
     2161 TTAAATGCTT CCTATATTAT ATATATAGTA ATGTCGTTTG GTGCACTCTC AGTACAATCT
     2221 GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT
     2281 GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT
     2341 GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG GGCCTCGTGA
     2401 TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA
     2461 CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA
     2521 TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA
     2581 GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC
     2641 CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG
     2701 CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC
     2761 CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT
     2821 CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT
     2881 TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT
     2941 TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA
     3001 TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TTCACAACAT GGGGGATCAT GTAACTCGCC
     3061 TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA
     3121 TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG
     3181 CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC
     3241 GCTCGGCCCT TCcggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     3301 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     3361 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     3421 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     3481 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     3541 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     3601 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3661 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3721 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     3781 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3841 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3901 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3961 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4021 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4081 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4141 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4201 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcggg
     4261 ggatctgcca attgaacata acatggtagt tacatatact agtaatatgg ttcggcacac
     4321 attaaaagta taaaaactat ctgaattacg aattacatat attggtcata aaaatcaatc
     4381 aatcatcgtg tgttttatat gtctcttatc taagtataag aatatccata gttaatattc
     4441 acttacgcta ccttttaacc tgtaatcatt gtcaacagga tatgttaacg acccacattg
     4501 ataaacgcta gtatttcttt ttcctcttct tattggccgg ctgtctctat actcccctat
     4561 agtctgtttc ttttcgtttc gattgtccct atcagtgata gagatggcgc acatggtacg
     4621 ctgtggtgct cgcggctggg aacgaaactc tgggagctgc gattggcagG GCGGTAAGTC
     4681 TATCTACGGg ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
     4741 tgaaaaagtg gcaccgagtc ggtgcttttt tctcgagcca tatccaactt ccaatttaat
     4801 ctttcttttt taattttcac ttatttgcga tacagaaaga aaaaagcgat agtaactatt
     4861 gaattttgtt tggatttggt tagattagat atggtttctc tttatattta catgctaaaa
     4921 atgggctaca ccagagatac ataattagat atatatacgc cagtacacct tatcggccca
     4981 agccttgtcc caaggcagcg ttttgttctt ggaaacgctg ccctacacgt tcgctatgct
     5041 tcaagaactt ttctgagcac ttcatgatgc atgtttgttc cttattggtt agctttgatg
     5101 ttgtgaagtc attgacacag tctgtgaaac atctttctac cagattagag tacaaacgca
     5161 tgaaatcctt catttgcttt tgttccacta ctttttggaa ctcttgttgt tctttCCTGC
     5221 AGGtagtcgt gcaatgtatg actttaagat ttgtgagcag gaagaaaagg gagaatcttc
     5281 taacgataaa cccttgaaaa actgggtaga ctacgctatg ttgagttgct acgcaggctg
     5341 cacaattaca cgagaatgct cccgcctagg atttaaggct aagggacgtg caatgcagac
     5401 gacagatcta aatgaccgtg tcggtgaagt gttcgccaaa cttttcggtt aacacatgca
     5461 gtgatgcacg cgcgatggtg ctaagttaca tatatatata tatatatata tatatatata
     5521 tatagccata gtgatgtcta agtaaccttt atggtatatt tcttaatgtg gaaagatact
     5581 agcgcgcgca cccacacaca agcttcgtct tttcttgaag aaaagaggaa gctcgctaaa
     5641 tgggattcca ctttccgttc cctgccagct gatggaaaaa ggttagtgga acgatgaaga
     5701 ataaaaagag agatccactg aggtgaaatt tcagctgaca gcgagtttca tgatcgtgat
     5761 gaacaatggt aacgagttgt ggctgttgcc agggagggtg gttctcaact tttaatgtat
     5821 ggccaaatcg ctacttgggt ttgttatata acaaagaaga aataatgaac tgattctctt
     5881 cctccttctt gtcctttctt aattctgttg taattacctt cctttgtaat tttttttgta
     5941 attattcttc ttaataatcc aaacaaacac acatattaca atatattaaa atgtctagat
     6001 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag
     6061 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc
     6121 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc
     6181 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta
     6241 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac
     6301 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag
     6361 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt
     6421 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg
     6481 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc
     6541 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg
     6601 aaagtgggtc ttaagtctga agaatgaatg atttgatgat ttctttttcc ctccattttt
     6661 cttactgaat atatcaatga tatagacttg tatagtttat tatttcaaat taagtagcta
     6721 tatatagtca agataacgtt tgtttgacac gattacatta ttcgtcgaca tcttttttca
     6781 gcctgtcgtg gtagcaattt gaggagtatt attaattgaa taggttcatt ttgcgctcgc
     6841 ataaacagtt ttcgtcaggg acagtatgtt ggaatgagtg gtaattaatg gtgacatgac
     6901 atgttatagc aataaccttg atgtttacat cgtagtttaa tgtacacccc gcgaattcgt
     6961 tcaagtagga gtgcaccaat tgcaaaggga aaagctgaat gggcagttcg aaCCGCGGtc
     7021 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7081 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     7141 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     7201 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttacctc
     7261 actcattagg caccccaggc tttacacttt atgcttccgg ctcctatgtt gtgtggaatt
     7321 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     7381 attaaccctc actaaaggga acaaaagctg gAGCTCATAG CTTCAAAATG TTTCTACTCC
     7441 TTTTTTACTC TTCCAGATTT TCTCGGACTC CGCGCATCGC CGTACCACTT CAAAACACCC
     7501 AAGCACAGCA TACTAAATTT CCCCTCTTTC TTCCTCTAGG GTGTCGTTAA TTACCCGTAC
     7561 TAAAGGTTTG GAAAAGAAAA AAGAGACCGC CTCGTTTCTT TTTCTTCGTC GAAAAAGGCA
     7621 ATAAAAATTT TTATCACGTT TCTTTTTCTT GAAAATTTTT TTTTTGATTT TTTTCTCTTT
     7681 CGATGACCTC CCATTGATAT TTAAGTTAAT AAACGGTCTT CAATTTCTCA AGTTTCAGTT
     7741 TCATTTTTCT TGTTCTATTA CAACTTTTTT TACTTCTTGC TCATTAGAAA GAAAGCATAG
     7801 CAATCTAATC TAAGTTTTCT AGAACTAGTG GATCCCCCGG GaaaaATGGA CAAGAAGTAC
     7861 TCCATTGGGC TCGATATCGG CACAAACAGC GTCGGtTGGG CCGTCATTAC GGACGAGTAC
     7921 AAGGTGCCGA GCAAAAAATT CAAAGTTCTG GGCAATACCG ATCGCCACAG CATAAAGAAG
     7981 AACCTCATTG GCGCCCTCCT GTTCGACTCC GGGGAGACGG CCGAAGCCAC GCGGCTCAAA
     8041 AGAACAGCAC GGCGCAGATA TACCCGCAGA AAGAATCGGA TCTGCTACCT GCAGGAGATC
     8101 TTTAGTAATG AGATGGCTAA GGTGGATGAC TCTTTCTTCC ATAGGCTGGA GGAGTCCTTT
     8161 TTGGTGGAGG AGGATAAAAA GCACGAGCGC CACCCAATCT TTGGCAATAT CGTGGACGAG
     8221 GTGGCGTACC ATGAAAAGTA CCCAACCATA TATCATCTGA GGAAGAAGCT TGTAGACAGT
     8281 ACTGATAAGG CTGACTTGCG GTTGATCTAT CTCGCGCTGG CGCATATGAT CAAATTTCGG
     8341 GGACACTTCC TCATCGAGGG GGACCTGAAC CCAGACAACA GCGATGTCGA CAAACTCTTT
     8401 ATCCAACTGG TTCAGACTTA CAATCAGCTT TTCGAAGAGA ACCCGATCAA CGCATCCGGA
     8461 GTTGACGCCA AAGCAATCCT GAGCGCTAGG CTGTCCAAAT CCCGGCGGCT CGAAAACCTC
     8521 ATCGCACAGC TCCCTGGGGA GAAGAAGAAC GGCCTGTTTG GTAATCTTAT CGCCCTGTCA
     8581 CTCGGGCTGA CCCCCAACTT TAAATCTAAC TTCGACCTGG CCGAAGATGC CAAGCTTCAA
     8641 CTGAGCAAAG ACACCTACGA TGATGATCTC GACAATCTGC TGGCCCAGAT CGGCGACCAG
     8701 TACGCAGACC TTTTTTTGGC GGCAAAGAAC CTGTCAGACG CCATTCTGCT GAGTGATATT
     8761 CTGCGAGTGA ACACGGAGAT CACCAAAGCT CCGCTGAGCG CTAGTATGAT CAAGCGCTAT
     8821 GATGAGCACC ACCAAGACTT GACTTTGCTG AAGGCCCTTG TCAGACAGCA ACTGCCTGAG
     8881 AAGTACAAGG AAATTTTCTT CGATCAGTCT AAAAATGGCT ACGCCGGATA CATTGACGGC
     8941 GGAGCAAGCC AGGAGGAATT TTACAAATTT ATTAAGCCCA TCTTGGAAAA AATGGACGGC
     9001 ACCGAGGAGC TGCTGGTAAA GCTTAACAGA GAAGATCTGT TGCGCAAACA GCGCACTTTC
     9061 GACAATGGAA GCATCCCCCA CCAGATTCAC CTGGGCGAAC TGCACGCTAT CCTCAGGCGG
     9121 CAAGAGGATT TCTACCCCTT TTTGAAAGAT AACAGGGAAA AGATTGAGAA AATCCTCACA
     9181 TTTCGGATAC CCTACTATGT AGGCCCCCTC GCCCGGGGAA ATTCCAGATT CGCGTGGATG
     9241 ACTCGCAAAT CAGAAGAGAC CATCACTCCC TGGAACTTCG AGGAAGTCGT GGATAAGGGG
     9301 GCCTCTGCCC AGTCCTTCAT CGAAAGGATG ACTAACTTTG ATAAAAATCT GCCTAACGAA
     9361 AAGGTGCTTC CTAAACACTC TCTGCTGTAC GAGTACTTCA CAGTTTATAA CGAGCTCACC
     9421 AAGGTCAAAT ACGTCACAGA AGGGATGAGA AAGCCAGCAT TCCTGTCTGG AGAGCAGAAG
     9481 AAAGCTATCG TGGACCTCCT CTTCAAGACG AACCGGAAAG TTACCGTGAA ACAGCTCAAA
     9541 GAAGACTATT TCAAAAAGAT TGAATGTTTC GACTCTGTTG AAATCAGCGG AGTGGAGGAT
     9601 CGCTTCAACG CATCCCTGGG AACGTATCAC GATCTCCTGA AAATCATTAA AGACAAGGAC
     9661 TTCCTGGACA ATGAGGAGAA CGAGGACATT CTTGAGGACA TTGTCCTCAC CCTTACGTTG
     9721 TTTGAAGATA GGGAGATGAT TGAAGAACGC TTGAAAACTT ACGCTCATCT CTTCGACGAC
     9781 AAAGTCATGA AACAGCTCAA GAGGCGCCGA TATACAGGAT GGGGGCGGCT GTCAAGAAAA
     9841 CTGATCAATG GGATCCGAGA CAAGCAGAGT GGAAAGACAA TCCTGGATTT TCTTAAGTCC
     9901 GATGGATTTG CCAACCGGAA CTTCATGCAG TTGATCCATG ATGACTCTCT CACCTTTAAG
     9961 GAGGACATCC AGAAAGCACA AGTTTCTGGC CAGGGGGACA GTCTTCACGA GCACATCGCT
    10021 AATCTTGCAG GTAGCCCAGC TATCAAAAAG GGAATACTGC AGACCGTTAA GGTCGTGGAT
    10081 GAACTCGTCA AAGTAATGGG AAGGCATAAG CCCGAGAATA TCGTTATCGA GATGGCCCGA
    10141 GAGAACCAAA CTACCCAGAA GGGACAGAAG AACAGTAGGG AAAGGATGAA GAGGATTGAA
    10201 GAGGGTATAA AAGAACTGGG GTCCCAAATC CTTAAGGAAC ACCCAGTTGA AAACACCCAG
    10261 CTTCAGAATG AGAAGCTCTA CCTGTACTAC CTGCAGAACG GCAGGGACAT GTACGTGGAT
    10321 CAGGAACTGG ACATCAATCG GCTCTCCGAC TACGACGTGG ATCATATCGT GCCCCAGTCT
    10381 TTTCTCAAAG ATGATTCTAT TGATAATAAA GTGTTGACAA GATCCGATAA AAATAGAGGG
    10441 AAGAGTGATA ACGTCCCCTC AGAAGAAGTT GTCAAGAAAA TGAAAAATTA TTGGCGGCAG
    10501 CTGCTGAACG CCAAACTGAT CACACAACGG AAGTTCGATA ATCTGACTAA GGCTGAACGA
    10561 GGTGGCCTGT CTGAGTTGGA TAAAGCCGGC TTCATCAAAA GGCAGCTTGT TGAGACACGC
    10621 CAGATCACCA AGCACGTGGC CCAAATTCTC GATTCACGCA TGAACACCAA GTACGATGAA
    10681 AATGACAAAC TGATTCGAGA GGTGAAAGTT ATTACTCTGA AGTCTAAGCT GGTCTCAGAT
    10741 TTCAGAAAGG ACTTTCAGTT TTATAAGGTG AGAGAGATCA ACAATTACCA CCATGCGCAT
    10801 GATGCCTACC TGAATGCAGT GGTAGGCACT GCACTTATCA AAAAATATCC CAAGCTTGAA
    10861 TCTGAATTTG TTTACGGAGA CTATAAAGTG TACGATGTTA GGAAAATGAT CGCAAAGTCT
    10921 GAGCAGGAAA TAGGCAAGGC CACCGCTAAG TACTTCTTTT ACAGCAATAT TATGAATTTT
    10981 TTCAAGACCG AGATTACACT GGCCAATGGA GAGATTCGGA AGCGACCACT TATCGAAACA
    11041 AACGGAGAAA CAGGAGAAAT CGTGTGGGAC AAGGGTAGGG ATTTCGCGAC AGTCCGGAAG
    11101 GTCCTGTCCA TGCCGCAGGT GAACATCGTT AAAAAGACCG AAGTACAGAC CGGAGGCTTC
    11161 TCCAAGGAAA GTATCCTCCC GAAAAGGAAC AGCGACAAGC TGATCGCACG CAAAAAAGAT
    11221 TGGGACCCCA AGAAATACGG CGGATTCGAT TCTCCTACAG TCGCTTACAG TGTACTGGTT
    11281 GTGGCCAAAG TGGAGAAAGG GAAGTCTAAA AAACTCAAAA GCGTCAAGGA ACTGCTGGGC
    11341 ATCACAATCA TGGAGCGATC AAGCTTCGAA AAAAACCCCA TCGACTTTCT CGAGGCGAAA
    11401 GGATATAAAG AGGTCAAAAA AGACCTCATC ATTAAGCTTC CCAAGTACTC TCTCTTTGAG
    11461 CTTGAAAACG GCCGGAAACG AATGCTCGCT AGTGCGGGCG AGCTGCAGAA AGGTAACGAG
    11521 CTGGCACTGC CCTCTAAATA CGTTAATTTC TTGTATCTGG CCAGCCACTA TGAAAAGCTC
    11581 AAAGGGTCTC CCGAAGATAA TGAGCAGAAG CAGCTGTTCG TGGAACAACA CAAACACTAC
    11641 CTTGATGAGA TCATCGAGCA AATAAGCGAA TTCTCCAAAA GAGTGATCCT CGCCGACGCT
    11701 AACCTCGATA AGGTGCTTTC TGCTTACAAT AAGCACAGGG ATAAGCCCAT CA
//