LOCUS       pNSU299-SLA1           11752 bp ds-DNA     circular     26-NOV-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     gene            complement(9529..10203)
                     /label="TRP1"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    4357..4379
                     /label="kozak rev"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    8496..8516
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     gene            4380..8495
                     /label="SpCas9"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_binding    3849..3871
                     /label="LacO"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Binding Site    1204..1223
                     /label="Guide RNA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      8539..8778
                     /label="CYC1_terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1119..1160
                     /label="RNAse P leader sequence"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    3950..4356
                     /label="TEF1 promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1120..1138
                     /label="TetO"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1119..1119
                     /label="TSS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     gene            10669..11729
                     /label="Ampicillin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     gene            complement(10204..10484)
                     /label="TRP1 prom"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             8904..8962
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             2525..3148
                     /label="TetR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      1312..1749
                     /label="RPR1t"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      72..754
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        792..1160
                     /label="pRPR1"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        1758..2517
                     /label="pGPM1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      3149..3546
                     /label="tGPM1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    8502..8516
                     /label="NLS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     rep_origin      8977..9432
                     /label="f1 ori"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1224..1305
                     /label="sgRNA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          3914..3933
                     /label="T3"
                     /note="sequence: AATTAACCCTCACTAAAGGG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          9350..9367
                     /label="pEGFP-C1-2800-R"
                     /note="sequence: GATTTCGGCCTATTGGTT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          10622..10640
                     /label="pGexR"
                     /note="sequence: GAGCTGCATGTGTCAGAGG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(1311..1335)
                     /label="RPR1-rev"
                     /note="sequence: gattaaattggaagttggatatggc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          3591..3610
                     /label="VR"
                     /note="sequence: ATTACCGCCTTTGAGTGAGC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(10727..10746)
                     /label="VF2"
                     /note="sequence: TGCCACCTGACGTCTAAGAA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          1010..1031
                     /label="RPR1-fwd"
                     /note="sequence: caggatatgttaacgacccaca"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(8833..8855)
                     /label="M13F43"
                     /note="sequence: AGGGTTTTCCCAGTCACGACGTT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(1224..1263)
                     /label="67-3'gRNA-extender"
                     /note="sequence: gactagccttattttaacttgctatttctagctctaaaac"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          1164..1203
                     /label="68-5'gRNA-extender"
                     /note="sequence: tcgcggctgggaacgaaactctgggagctgcgattggcag"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          3877..3894
                     /label="M13R"
                     /note="sequence: CAGGAAACAGCTATGACC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(8907..8928)
                     /label="pNSU299-3546-REV"
                     /note="sequence: tgcgggcctcttcgctattacg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(8816..8833)
                     /label="M13F"
                     /note="sequence: TGTAAAACGACGGCCAGT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          8837..8858
                     /label="pNSU299-3455-FWD"
                     /note="sequence: tcgtgactgggaaaaccctggc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(10668..10689)
                     /label="pQEfPR"
                     /note="sequence: GTATCACGAGGCCCTTTCGTCT"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          3810..3835
                     /label="pexR"
                     /note="sequence: CAGGCTTTACACTTTATGCTTCCGGC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(8548..8566)
                     /label="CYC1Reverse"
                     /note="sequence: GCGTGAATGTAAGCGTGAC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          3857..3880
                     /label="M13R49"
                     /note="sequence: GAGCGGATAACAATTTCACACAGG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(10564..10584)
                     /label="pexF"
                     /note="sequence: GGAGCAGACAAGCCCGTCAGG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(8787..8806)
                     /label="T7"
                     /note="sequence: TAATACGACTCACTATAGGG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
       61 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      121 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      181 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      241 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
      301 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      361 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      421 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      481 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      541 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      601 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      661 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      721 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      781 gccttttgct cgggggatct gccaattgaa cataacatgg tagttacata tactagtaat
      841 atggttcggc acacattaaa agtataaaaa ctatctgaat tacgaattac atatattggt
      901 cataaaaatc aatcaatcat cgtgtgtttt atatgtctct tatctaagta taagaatatc
      961 catagttaat attcacttac gctacctttt aacctgtaat cattgtcaac aggatatgtt
     1021 aacgacccac attgataaac gctagtattt ctttttcctc ttcttattgg ccggctgtct
     1081 ctatactccc ctatagtctg tttcttttcg tttcgattgt ccctatcagt gatagagatg
     1141 gcgcacatgg tacgctgtgg TGCtcgcggc tgggaacgaa actctgggag ctgcgattgg
     1201 cagATGATGC CAATGATTAC TGGgttttag agctagaaat agcaagttaa aataaggcta
     1261 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttctcga gccatatcca
     1321 acttccaatt taatctttct tttttaattt tcacttattt gcgatacaga aagaaaaaag
     1381 cgatagtaac tattgaattt tgtttggatt tggttagatt agatatggtt tctctttata
     1441 tttacatgct aaaaatgggc tacaccagag atacataatt agatatatat acgccagtac
     1501 accttatcgg cccaagcctt gtcccaaggc agcgttttgt tcttggaaac gctgccctac
     1561 acgttcgcta tgcttcaaga acttttctga gcacttcatg atgcatgttt gttccttatt
     1621 ggttagcttt gatgttgtga agtcattgac acagtctgtg aaacatcttt ctaccagatt
     1681 agagtacaaa cgcatgaaat ccttcatttg cttttgttcc actacttttt ggaactcttg
     1741 ttgttctttC CTGCAGGtag tcgtgcaatg tatgacttta agatttgtga gcaggaagaa
     1801 aagggagaat cttctaacga taaacccttg aaaaactggg tagactacgc tatgttgagt
     1861 tgctacgcag gctgcacaat tacacgagaa tgctcccgcc taggatttaa ggctaaggga
     1921 cgtgcaatgc agacgacaga tctaaatgac cgtgtcggtg aagtgttcgc caaacttttc
     1981 ggttaacaca tgcagtgatg cacgcgcgat ggtgctaagt tacatatata tatatatata
     2041 tatatatata tatatatagc catagtgatg tctaagtaac ctttatggta tatttcttaa
     2101 tgtggaaaga tactagcgcg cgcacccaca cacaagcttc gtcttttctt gaagaaaaga
     2161 ggaagctcgc taaatgggat tccactttcc gttccctgcc agctgatgga aaaaggttag
     2221 tggaacgatg aagaataaaa agagagatcc actgaggtga aatttcagct gacagcgagt
     2281 ttcatgatcg tgatgaacaa tggtaacgag ttgtggctgt tgccagggag ggtggttctc
     2341 aacttttaat gtatggccaa atcgctactt gggtttgtta tataacaaag aagaaataat
     2401 gaactgattc tcttcctcct tcttgtcctt tcttaattct gttgtaatta ccttcctttg
     2461 taattttttt tgtaattatt cttcttaata atccaaacaa acacacatat tacaatatat
     2521 taaaatgtct agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga
     2581 ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc
     2641 tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat
     2701 gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt
     2761 acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt
     2821 acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt
     2881 tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca
     2941 ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga
     3001 aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca
     3061 ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa
     3121 acaacttaaa tgtgaaagtg ggtcttaagt ctgaagaatg aatgatttga tgatttcttt
     3181 ttccctccat ttttcttact gaatatatca atgatataga cttgtatagt ttattatttc
     3241 aaattaagta gctatatata gtcaagataa cgtttgtttg acacgattac attattcgtc
     3301 gacatctttt ttcagcctgt cgtggtagca atttgaggag tattattaat tgaataggtt
     3361 cattttgcgc tcgcataaac agttttcgtc agggacagta tgttggaatg agtggtaatt
     3421 aatggtgaca tgacatgtta tagcaataac cttgatgttt acatcgtagt ttaatgtaca
     3481 ccccgcgaat tcgttcaagt aggagtgcac caattgcaaa gggaaaagct gaatgggcag
     3541 ttcgaaCCGC GGtctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     3601 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     3661 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     3721 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     3781 atgtgagtta cctcactcat taggcacccc aggctttaca ctttatgctt ccggctccta
     3841 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     3901 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggAGCTC ATAGCTTCAA
     3961 AATGTTTCTA CTCCTTTTTT ACTCTTCCAG ATTTTCTCGG ACTCCGCGCA TCGCCGTACC
     4021 ACTTCAAAAC ACCCAAGCAC AGCATACTAA ATTTCCCCTC TTTCTTCCTC TAGGGTGTCG
     4081 TTAATTACCC GTACTAAAGG TTTGGAAAAG AAAAAAGAGA CCGCCTCGTT TCTTTTTCTT
     4141 CGTCGAAAAA GGCAATAAAA ATTTTTATCA CGTTTCTTTT TCTTGAAAAT TTTTTTTTTG
     4201 ATTTTTTTCT CTTTCGATGA CCTCCCATTG ATATTTAAGT TAATAAACGG TCTTCAATTT
     4261 CTCAAGTTTC AGTTTCATTT TTCTTGTTCT ATTACAACTT TTTTTACTTC TTGCTCATTA
     4321 GAAAGAAAGC ATAGCAATCT AATCTAAGTT TTCTAGAACT AGTGGATCCC CCGGGaaaaA
     4381 TGGACAAGAA GTACTCCATT GGGCTCGATA TCGGCACAAA CAGCGTCGGt TGGGCCGTCA
     4441 TTACGGACGA GTACAAGGTG CCGAGCAAAA AATTCAAAGT TCTGGGCAAT ACCGATCGCC
     4501 ACAGCATAAA GAAGAACCTC ATTGGCGCCC TCCTGTTCGA CTCCGGGGAG ACGGCCGAAG
     4561 CCACGCGGCT CAAAAGAACA GCACGGCGCA GATATACCCG CAGAAAGAAT CGGATCTGCT
     4621 ACCTGCAGGA GATCTTTAGT AATGAGATGG CTAAGGTGGA TGACTCTTTC TTCCATAGGC
     4681 TGGAGGAGTC CTTTTTGGTG GAGGAGGATA AAAAGCACGA GCGCCACCCA ATCTTTGGCA
     4741 ATATCGTGGA CGAGGTGGCG TACCATGAAA AGTACCCAAC CATATATCAT CTGAGGAAGA
     4801 AGCTTGTAGA CAGTACTGAT AAGGCTGACT TGCGGTTGAT CTATCTCGCG CTGGCGCATA
     4861 TGATCAAATT TCGGGGACAC TTCCTCATCG AGGGGGACCT GAACCCAGAC AACAGCGATG
     4921 TCGACAAACT CTTTATCCAA CTGGTTCAGA CTTACAATCA GCTTTTCGAA GAGAACCCGA
     4981 TCAACGCATC CGGAGTTGAC GCCAAAGCAA TCCTGAGCGC TAGGCTGTCC AAATCCCGGC
     5041 GGCTCGAAAA CCTCATCGCA CAGCTCCCTG GGGAGAAGAA GAACGGCCTG TTTGGTAATC
     5101 TTATCGCCCT GTCACTCGGG CTGACCCCCA ACTTTAAATC TAACTTCGAC CTGGCCGAAG
     5161 ATGCCAAGCT TCAACTGAGC AAAGACACCT ACGATGATGA TCTCGACAAT CTGCTGGCCC
     5221 AGATCGGCGA CCAGTACGCA GACCTTTTTT TGGCGGCAAA GAACCTGTCA GACGCCATTC
     5281 TGCTGAGTGA TATTCTGCGA GTGAACACGG AGATCACCAA AGCTCCGCTG AGCGCTAGTA
     5341 TGATCAAGCG CTATGATGAG CACCACCAAG ACTTGACTTT GCTGAAGGCC CTTGTCAGAC
     5401 AGCAACTGCC TGAGAAGTAC AAGGAAATTT TCTTCGATCA GTCTAAAAAT GGCTACGCCG
     5461 GATACATTGA CGGCGGAGCA AGCCAGGAGG AATTTTACAA ATTTATTAAG CCCATCTTGG
     5521 AAAAAATGGA CGGCACCGAG GAGCTGCTGG TAAAGCTTAA CAGAGAAGAT CTGTTGCGCA
     5581 AACAGCGCAC TTTCGACAAT GGAAGCATCC CCCACCAGAT TCACCTGGGC GAACTGCACG
     5641 CTATCCTCAG GCGGCAAGAG GATTTCTACC CCTTTTTGAA AGATAACAGG GAAAAGATTG
     5701 AGAAAATCCT CACATTTCGG ATACCCTACT ATGTAGGCCC CCTCGCCCGG GGAAATTCCA
     5761 GATTCGCGTG GATGACTCGC AAATCAGAAG AGACCATCAC TCCCTGGAAC TTCGAGGAAG
     5821 TCGTGGATAA GGGGGCCTCT GCCCAGTCCT TCATCGAAAG GATGACTAAC TTTGATAAAA
     5881 ATCTGCCTAA CGAAAAGGTG CTTCCTAAAC ACTCTCTGCT GTACGAGTAC TTCACAGTTT
     5941 ATAACGAGCT CACCAAGGTC AAATACGTCA CAGAAGGGAT GAGAAAGCCA GCATTCCTGT
     6001 CTGGAGAGCA GAAGAAAGCT ATCGTGGACC TCCTCTTCAA GACGAACCGG AAAGTTACCG
     6061 TGAAACAGCT CAAAGAAGAC TATTTCAAAA AGATTGAATG TTTCGACTCT GTTGAAATCA
     6121 GCGGAGTGGA GGATCGCTTC AACGCATCCC TGGGAACGTA TCACGATCTC CTGAAAATCA
     6181 TTAAAGACAA GGACTTCCTG GACAATGAGG AGAACGAGGA CATTCTTGAG GACATTGTCC
     6241 TCACCCTTAC GTTGTTTGAA GATAGGGAGA TGATTGAAGA ACGCTTGAAA ACTTACGCTC
     6301 ATCTCTTCGA CGACAAAGTC ATGAAACAGC TCAAGAGGCG CCGATATACA GGATGGGGGC
     6361 GGCTGTCAAG AAAACTGATC AATGGGATCC GAGACAAGCA GAGTGGAAAG ACAATCCTGG
     6421 ATTTTCTTAA GTCCGATGGA TTTGCCAACC GGAACTTCAT GCAGTTGATC CATGATGACT
     6481 CTCTCACCTT TAAGGAGGAC ATCCAGAAAG CACAAGTTTC TGGCCAGGGG GACAGTCTTC
     6541 ACGAGCACAT CGCTAATCTT GCAGGTAGCC CAGCTATCAA AAAGGGAATA CTGCAGACCG
     6601 TTAAGGTCGT GGATGAACTC GTCAAAGTAA TGGGAAGGCA TAAGCCCGAG AATATCGTTA
     6661 TCGAGATGGC CCGAGAGAAC CAAACTACCC AGAAGGGACA GAAGAACAGT AGGGAAAGGA
     6721 TGAAGAGGAT TGAAGAGGGT ATAAAAGAAC TGGGGTCCCA AATCCTTAAG GAACACCCAG
     6781 TTGAAAACAC CCAGCTTCAG AATGAGAAGC TCTACCTGTA CTACCTGCAG AACGGCAGGG
     6841 ACATGTACGT GGATCAGGAA CTGGACATCA ATCGGCTCTC CGACTACGAC GTGGATCATA
     6901 TCGTGCCCCA GTCTTTTCTC AAAGATGATT CTATTGATAA TAAAGTGTTG ACAAGATCCG
     6961 ATAAAAATAG AGGGAAGAGT GATAACGTCC CCTCAGAAGA AGTTGTCAAG AAAATGAAAA
     7021 ATTATTGGCG GCAGCTGCTG AACGCCAAAC TGATCACACA ACGGAAGTTC GATAATCTGA
     7081 CTAAGGCTGA ACGAGGTGGC CTGTCTGAGT TGGATAAAGC CGGCTTCATC AAAAGGCAGC
     7141 TTGTTGAGAC ACGCCAGATC ACCAAGCACG TGGCCCAAAT TCTCGATTCA CGCATGAACA
     7201 CCAAGTACGA TGAAAATGAC AAACTGATTC GAGAGGTGAA AGTTATTACT CTGAAGTCTA
     7261 AGCTGGTCTC AGATTTCAGA AAGGACTTTC AGTTTTATAA GGTGAGAGAG ATCAACAATT
     7321 ACCACCATGC GCATGATGCC TACCTGAATG CAGTGGTAGG CACTGCACTT ATCAAAAAAT
     7381 ATCCCAAGCT TGAATCTGAA TTTGTTTACG GAGACTATAA AGTGTACGAT GTTAGGAAAA
     7441 TGATCGCAAA GTCTGAGCAG GAAATAGGCA AGGCCACCGC TAAGTACTTC TTTTACAGCA
     7501 ATATTATGAA TTTTTTCAAG ACCGAGATTA CACTGGCCAA TGGAGAGATT CGGAAGCGAC
     7561 CACTTATCGA AACAAACGGA GAAACAGGAG AAATCGTGTG GGACAAGGGT AGGGATTTCG
     7621 CGACAGTCCG GAAGGTCCTG TCCATGCCGC AGGTGAACAT CGTTAAAAAG ACCGAAGTAC
     7681 AGACCGGAGG CTTCTCCAAG GAAAGTATCC TCCCGAAAAG GAACAGCGAC AAGCTGATCG
     7741 CACGCAAAAA AGATTGGGAC CCCAAGAAAT ACGGCGGATT CGATTCTCCT ACAGTCGCTT
     7801 ACAGTGTACT GGTTGTGGCC AAAGTGGAGA AAGGGAAGTC TAAAAAACTC AAAAGCGTCA
     7861 AGGAACTGCT GGGCATCACA ATCATGGAGC GATCAAGCTT CGAAAAAAAC CCCATCGACT
     7921 TTCTCGAGGC GAAAGGATAT AAAGAGGTCA AAAAAGACCT CATCATTAAG CTTCCCAAGT
     7981 ACTCTCTCTT TGAGCTTGAA AACGGCCGGA AACGAATGCT CGCTAGTGCG GGCGAGCTGC
     8041 AGAAAGGTAA CGAGCTGGCA CTGCCCTCTA AATACGTTAA TTTCTTGTAT CTGGCCAGCC
     8101 ACTATGAAAA GCTCAAAGGG TCTCCCGAAG ATAATGAGCA GAAGCAGCTG TTCGTGGAAC
     8161 AACACAAACA CTACCTTGAT GAGATCATCG AGCAAATAAG CGAATTCTCC AAAAGAGTGA
     8221 TCCTCGCCGA CGCTAACCTC GATAAGGTGC TTTCTGCTTA CAATAAGCAC AGGGATAAGC
     8281 CCATCAGGGA GCAGGCAGAA AACATTATCC ACTTGTTTAC TCTGACCAAC TTGGGCGCGC
     8341 CTGCAGCCTT CAAGTACTTC GACACCACCA TAGACAGAAA GCGGTACACC TCTACAAAGG
     8401 AGGTCCTGGA CGCCACACTG ATTCATCAGT CAATTACGGG GCTCTATGAA ACAAGAATCG
     8461 ACCTCTCTCA GCTCGGTGGA GACAGCAGGG CTGACCCCAA GAAGAAGAGG AAGGTGTGAT
     8521 CTCTTCTCGA GTCATGTAAT TAGTTATGTC ACGCTTACAT TCACGCCCTC CCCCCACATC
     8581 CGCTCTAACC GAAAAGGAAG GAGTTAGACA ACCTGAAGTC TAGGTCCCTA TTTATTTTTT
     8641 TATAGTTATG TTAGTATTAA GAACGTTATT TATATTTCAA ATTTTTCTTT TTTTTCTGTA
     8701 CAGACGCGTG TACGCATGTA ACATTATACT GAAAACCTTG CTTGAGAAGG TTTTGGGACG
     8761 CTCGAAGGCT TTAATTTGcc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     8821 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     8881 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     8941 cccaacagtt gcgcagcctg aaTGGCGAAT GGCGCGACGC GCCCTGTAGC GGCGCATTAA
     9001 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC
     9061 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG
     9121 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA
     9181 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC
     9241 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA
     9301 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT
     9361 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA
     9421 CGTTTACAAT TTCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG
     9481 CAGGCAAGTG CACAAACAAT ACTTAAATAA ATACTACTCA GTAATAACCT ATTTCTTAGC
     9541 ATTTTTGACG AAATTTGCTA TTTTGTTAGA GTCTTTTACA CCATTTGTCT CCACACCTCC
     9601 GCTTACATCA ACACCAATAA CGCCATTTAA TCTAAGCGCA TCACCAACAT TTTCTGGCGT
     9661 CAGTCCACCA GCTAACATAA AATGTAAGCT TTCGGGGCTC TCTTGCCTTC CAACCCAGTC
     9721 AGAAATCGAG TTCCAATCCA AAAGTTCACC TGTCCCACCT GCTTCTGAAT CAAACAAGGG
     9781 AATAAACGAA TGAGGTTTCT GTGAAGCTGC ACTGAGTAGT ATGTTGCAGT CTTTTGGAAA
     9841 TACGAGTCTT TTAATAACTG GCAAACCGAG GAACTCTTGG TATTCTTGCC ACGACTCATC
     9901 TCCATGCAGT TGGACGATAT CAATGCCGTA ATCATTGACC AGAGCCAAAA CATCCTCCTT
     9961 AGGTTGATTA CGAAACACGC CAACCAAGTA TTTCGGAGTG CCTGAACTAT TTTTATATGC
    10021 TTTTACAAGA CTTGAAATTT TCCTTGCAAT AACCGGGTCA ATTGTTCTCT TTCTATTGGG
    10081 CACACATATA ATACCCAGCA AGTCAGCATC GGAATCTAGA GCACATTCTG CGGCCTCTGT
    10141 GCTCTGCAAG CCGCAAACTT TCACCAATGG ACCAGAACTA CCTGTGAAAT TAATAACAGA
    10201 CATACTCCAA GCTGCCTTTG TGTGCTTAAT CACGTATACT CACGTGCTCA ATAGTCACCA
    10261 ATGCCCTCCC TCTTGGCCCT CTCCTTTTCT TTTTTCGACC GAATTAATTC TTAATCGGCA
    10321 AAAAAAGAAA AGCTCCGGAT CAAGATTGTA CGTAAGGTGA CAAGCTATTT TTCAATAAAG
    10381 AATATCTTCC ACTACTGCCA TCTGGCGTCA TAACTGCAAA GTACACATAT ATTACGATGC
    10441 TGTCTATTAA ATGCTTCCTA TATTATATAT ATAGTAATGT CGTTTGGTGC ACTCTCAGTA
    10501 CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG
    10561 CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG
    10621 GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA CGAAAGGGCC
    10681 TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG
    10741 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
    10801 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
    10861 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
    10921 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
    10981 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
    11041 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
    11101 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
    11161 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
    11221 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
    11281 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTTCA CAACATGGGG GATCATGTAA
    11341 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
    11401 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
    11461 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
    11521 TTCTGCGCTC GGCCCTTCcg gctggctggt ttattgctga taaatctgga gccggtgagc
    11581 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    11641 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    11701 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca ta
//