LOCUS       pRR2                   10219 bp ds-DNA     circular     21-JAN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(254..764)
                     /label="CEN-ARS pRS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      1851..2533
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     complement(8515..8535)
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     mutation        4164..4166
                     /label="D10A"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     coding sequence 4140..8243
                     /label="dCas9 "
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3426..3440
                     /label="EK"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      8257..8496
                     /label="CYC1 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     2939..2958
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1094..1753
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     2901..2921
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(8544..8561)
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      8705..9160
                     /label="M13 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        2976..3402
                     /label="GAL1 promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     NLS             3447..3467
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_binding    2873..2895
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      8722..9028
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3504..4091
                     /label="FOKI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             3418..4139
                     /label="FokI-dCas9"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    6654..6656
                     /label="H840A"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     tag             3418..3440
                     /label="FLAG"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        2938..2956
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     gene            complement(9213..10035)
                     /label="TRP1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          2834..2859
                     /label="pexR"
                     /note="sequence: CAGGCTTTACACTTTATGCTTCCGGC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(8544..8561)
                     /label="M13F"
                     /note="sequence: TGTAAAACGACGGCCAGT"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(77..97)
                     /label="pexF"
                     /note="sequence: GGAGCAGACAAGCCCGTCAGG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(4115..4164)
                     /label="FokI-dCas9 REV"
                     /note="sequence: CCAAACCAATAGAGTATTTCTTATCACTTTCGGGTGTGGCGGACTCTGAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(8567..8592)
                     /label="c-klURA3"
                     /note="sequence: cacggcgcgcctagcagcggTAACGCCAGGGTTTTCCCAGTCAC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(4354..4379)
                     /label="SbfI-pTPG1-REV"
                     /note="sequence: TAACCTGCAGGTAGCAGATTCTGTTCTTTCTTCTGG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(8219..8243)
                     /label="pTPGI_dCas9 REV"
                     /note="sequence: CTAATTACATGACTCGAGAAGAGATTTAATCACCACCTAATTGAGACAAG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          3418..3447
                     /label="FokI-dCas9 FWD2"
                     /note="sequence: GAGAAAAAACCCCGGATTCTAGAACGATTACAAGGATGACGATGACAAGATGGCCC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(6801..6821)
                     /label="Cas9SeqPrimer3"
                     /note="sequence: GCCAAGTTGATCACCCAAAGA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          1354..1375
                     /label="ampR Bg1 fwd"
                     /note="sequence: gggggatcatgtaactcgcctt"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(8221..8268)
                     /label="pTPG_dCas9 REV2"
                     /note="sequence: GACATAACTAATTACATGACTCGAGTTAATCACCACCTAATTGAGACA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          4115..4164
                     /label="pTPGI_dCas9 FWD"
                     /note="sequence: CTCAGAGTCCGCCACACCCGAAAGTGATAAGAAATACTCTATTGGTTTGG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          8244..8266
                     /label="p415-GalL FWD"
                     /note="sequence: CTTGTCTCAATTAGGTGGTGATTAAATCTCTTCTCGAGTCATGTAATTAGTTATG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          3418..3445
                     /label="FokI-dCas9 FWD"
                     /note="sequence: AACTAGTGGATCCCCCGGGaaaaaaGATTACAAGGATGACGATGACAAGATGGC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          2870..2892
                     /label="newLAC-R"
                     /note="sequence: CCACATGTGGAATTGTGAGCGGATAACA"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          9078..9095
                     /label="pEGFP-C1-2800-R"
                     /note="sequence: GATTTCGGCCTATTGGTT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          4284..4302
                     /label="Cas9SeqPrimer1"
                     /note="sequence: GCCTTGTTGTTCGATTCTG"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(8515..8534)
                     /label="T7"
                     /note="sequence: TAATACGACTCACTATAGGG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          5698..5718
                     /label="Cas9SeqPrimer5"
                     /note="sequence: ACAACGAATTGACTAAGGTTA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          4140..4166
                     /label="SpeI_pTPGI_FWD"
                     /note="sequence: CTCAACTAGTATGGATAAGAAATACTCTATTGGTTTGGCT"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(8266..8284)
                     /label="CYC1Reverse"
                     /note="sequence: GCGTGAATGTAAGCGTGAC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          2615..2634
                     /label="VR"
                     /note="sequence: ATTACCGCCTTTGAGTGAGC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(8561..8583)
                     /label="M13F43"
                     /note="sequence: AGGGTTTTCCCAGTCACGACGTT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          2901..2918
                     /label="M13R"
                     /note="sequence: CAGGAAACAGCTATGACC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(181..202)
                     /label="pQEfPR"
                     /note="sequence: GTATCACGAGGCCCTTTCGTCT"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          8219..8271
                     /label="p415-GalL FWD2"
                     /note="sequence: CTTGTCTCAATTAGGTGGTGATTAACTCGAGTCATGTAATTAGTTATGTCACG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          2881..2904
                     /label="M13R49"
                     /note="sequence: GAGCGGATAACAATTTCACACAGG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(3392..3416)
                     /label="p415-GalL REV2"
                     /note="sequence: TCTTGTCATCGTCATCCTTGTAATCGTTCTAGAATCCGGGGTTTTTTCTC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          135..153
                     /label="pGexR"
                     /note="sequence: GAGCTGCATGTGTCAGAGG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          2944..2968
                     /label="d-klURA3"
                     /note="sequence: gtcagcggccgcatccctgcCCTCACTAAAGGGAACAAAAGCTG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(1718..1739)
                     /label="ampR Bg1 rev"
                     /note="sequence: gtgaggcacctatctcagcgat"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2938..2957
                     /label="T3"
                     /note="sequence: AATTAACCCTCACTAAAGGG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
       61 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
      121 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
      181 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      241 tcttaggacg gatcgcttgc ctgtaactta cacgcgcctc gtatctttta atgatggaat
      301 aatttgggaa tttactctgt gtttatttat ttttatgttt tgtatttgga ttttagaaag
      361 taaataaaga aggtagaaga gttacggaat gaagaaaaaa aaataaacaa aggtttaaaa
      421 aatttcaaca aaaagcgtac tttacatata tatttattag acaagaaaag cagattaaat
      481 agatatacat tcgattaacg ataagtaaaa tgtaaaatca caggattttc gtgtgtggtc
      541 ttctacacag acaagatgaa acaattcggc attaatacct gagagcagga agagcaagat
      601 aaaaggtagt atttgttggc gatcccccta gagtctttta catcttcgga aaacaaaaac
      661 tattttttct ttaatttctt tttttacttt ctatttttaa tttatatatt tatattaaaa
      721 aatttaaatt ataattattt ttatagcacg tgatgaaaag gacccaggtg gcacttttcg
      781 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      841 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      901 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      961 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1021 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     1081 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     1141 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     1201 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     1261 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1321 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1381 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     1441 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1501 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1561 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1621 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1681 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1741 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1801 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1861 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1921 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1981 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2041 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     2101 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2161 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2221 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2281 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2341 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2401 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2461 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2521 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2581 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     2641 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     2701 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     2761 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttacctcac
     2821 tcattaggca ccccaggctt tacactttat gcttccggct cctatgttgt gtggaattgt
     2881 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     2941 taaccctcac taaagggaac aaaagctggA GCTCGTGACA GCCCTCCGAA GGAAGACTCT
     3001 CCTCCGTGCG TCCTCGTCTT CACCGGTCGC GTTCCTGAAA CGCAGATGTG CCTCGCGCCG
     3061 CACTGCTCCG AACAATAAAG ATTCTACAAT ACTAGCTTTT ATGGTTATGA AGAGGAAAAA
     3121 TTGGCAGTAA CCTGGCCCCA CAAACCTTCA AATGAACGAA TCAAATTAAC AACCATAGGA
     3181 TGATAATGCG ATTAGTTTTT TAGCCTTATT TCTGGGGTAA TTAATCAGCG AAGCGATGAT
     3241 TTTTGATCTA TTAACGGATA TATAAATGCA AAAACTGCAT AACCACTTTA ACTAATACTT
     3301 TCAACATTTT CGGTTTGTAT TACTTCTTAT TCAAATGTAA TAAAAGTATC AACAAAAAAT
     3361 TGTTAATATA CCTCTATACT TTAACGTCAA GGAGAAAAAA CCCCGGATTC TAGAACTATT
     3421 ACAAGGATGA CGATGACAAG ATGGCCCCCA AGAAGAAGAG GAAGGTGGGC ATTCACCGCG
     3481 GGGTACCTGG AGGTTCTGGA TCCCAACTAG TCAAAAGTGA ACTGGAGGAG AAGAAATCTG
     3541 AACTTCGTCA TAAATTGAAA TATGTGCCTC ATGAATATAT TGAATTAATT GAAATTGCCA
     3601 GAAATTCCAC TCAGGATAGA ATTCTTGAAA TGAAGGTAAT GGAATTTTTT ATGAAAGTTT
     3661 ATGGATATAG AGGTAAACAT TTGGGTGGAT CAAGGAAACC GGACGGAGCA ATTTATACTG
     3721 TCGGATCTCC TATTGATTAC GGTGTGATCG TGGATACTAA AGCTTATAGC GGAGGTTATA
     3781 ATCTGCCAAT TGGCCAAGCA GATGAAATGC AACGATATGT CGAAGAAAAT CAAACACGAA
     3841 ACAAACATAT CAACCCTAAT GAATGGTGGA AAGTCTATCC ATCTTCTGTA ACGGAATTTA
     3901 AGTTTTTATT TGTGAGTGGT CACTTTAAAG GAAACTACAA AGCTCAGCTT ACACGATTAA
     3961 ATCATATCAC TAATTGTAAT GGAGCTGTTC TTAGTGTAGA AGAGCTTTTA ATTGGTGGAG
     4021 AAATGATTAA AGCCGGCACA TTAACCTTAG AGGAAGTCAG ACGGAAATTT AATAACGGCG
     4081 AGATAAACTT TAGCGGCAGC GAGACTCCCG GGACCTCAGA GTCCGCCACA CCCGAAAGTG
     4141 ATAAGAAATA CTCTATTGGT TTGGCTATCG GTACAAACTC TGTTGGTTGG GCTGTTATTA
     4201 CTGATGAATA CAAGGTTCCA TCCAAGAAGT TCAAGGTTTT GGGTAACACT GATAGACACT
     4261 CCATCAAAAA GAACTTGATT GGTGCCTTGT TGTTCGATTC TGGTGAAACT GCTGAAGCTA
     4321 CTAGATTGAA AAGAACCGCT AGAAGAAGAT ACACCAGAAG AAAGAACAGA ATCTGCTACT
     4381 TGCAAGAAAT CTTCTCCAAC GAAATGGCCA AGGTTGATGA TTCATTCTTC CACAGATTGG
     4441 AAGAATCCTT CTTGGTCGAA GAAGATAAGA AGCACGAAAG ACATCCAATC TTCGGTAACA
     4501 TCGTTGATGA AGTTGCTTAC CACGAAAAGT ACCCAACTAT CTACCATTTG AGAAAGAAGT
     4561 TGGTTGACTC TACCGATAAG GCTGATTTGA GATTGATCTA TTTGGCTTTG GCCCACATGA
     4621 TTAAGTTCAG AGGTCATTTC TTGATCGAAG GTGATTTGAA CCCAGATAAC TCCGATGTTG
     4681 ATAAGTTGTT CATCCAATTA GTCCAAACCT ACAATCAATT ATTCGAAGAA AACCCAATCA
     4741 ACGCCTCTGG TGTTGATGCT AAAGCTATTT TGTCTGCCAG ATTGTCCAAG TCCAGAAGAT
     4801 TAGAAAATTT GATCGCCCAA TTACCAGGTG AAAAGAAGAA TGGTTTGTTC GGTAATTTGA
     4861 TTGCCTTGTC TTTGGGTTTG ACTCCAAACT TCAAGTCCAA TTTCGATTTG GCTGAAGATG
     4921 CCAAGTTGCA ATTATCTAAG GATACCTACG ATGACGATTT GGATAACTTG TTGGCTCAAA
     4981 TCGGTGATCA ATACGCTGAT TTGTTTTTGG CTGCTAAGAA CTTGTCCGAT GCCATTTTGT
     5041 TGTCCGATAT TTTGAGAGTC AACACCGAAA TTACTAAGGC TCCATTGTCT GCCTCTATGA
     5101 TCAAAAGATA CGATGAACAC CACCAAGACT TGACTTTGTT GAAGGCTTTG GTCAGACAAC
     5161 AATTACCTGA AAAGTACAAA GAAATTTTCT TCGATCAATC CAAGAACGGT TACGCCGGTT
     5221 ATATTGATGG TGGTGCTTCT CAAGAAGAAT TTTACAAGTT CATCAAGCCA ATCTTGGAAA
     5281 AGATGGACGG TACTGAAGAA TTATTGGTCA AGTTGAACAG AGAAGATTTG TTGAGAAAGC
     5341 AAAGAACCTT CGACAACGGT TCTATTCCAC ATCAAATTCA CTTGGGTGAA TTGCACGCAA
     5401 TTTTGAGAAG ACAAGAAGAT TTTTATCCAT TCTTGAAGGA CAACAGAGAA AAGATCGAAA
     5461 AGATTCTGAC CTTCAGAATC CCTTACTACG TTGGTCCATT GGCTAGAGGT AATTCAAGAT
     5521 TTGCCTGGAT GACTAGAAAG TCCGAAGAAA CTATTACTCC TTGGAACTTC GAAGAAGTTG
     5581 TAGATAAGGG TGCTTCTGCC CAATCCTTTA TTGAAAGAAT GACCAACTTC GACAAGAACT
     5641 TGCCAAACGA AAAGGTTTTG CCAAAGCACT CTTTGTTGTA CGAATACTTC ACCGTCTACA
     5701 ACGAATTGAC TAAGGTTAAG TACGTCACCG AAGGTATGAG AAAACCAGCT TTTTTATCCG
     5761 GTGAACAAAA GAAGGCTATC GTCGATTTGT TGTTCAAGAC CAACAGAAAG GTTACTGTCA
     5821 AGCAATTAAA AGAAGATTAC TTCAAGAAAA TCGAATGCTT CGACTCCGTT GAAATTTCTG
     5881 GTGTCGAAGA TAGATTCAAT GCCTCTTTAG GTACTTACCA TGACTTGTTG AAAATCATCA
     5941 AGGACAAGGA TTTCTTGGAC AACGAAGAAA ACGAAGATAT TTTGGAAGAT ATTGTCTTGA
     6001 CATTGACCTT GTTTGAAGAT AGAGAAATGA TTGAAGAAAG ATTGAAAACC TACGCCCACT
     6061 TGTTCGATGA TAAGGTTATG AAGCAATTAA AGAGAAGAAG ATACACTGGT TGGGGTAGAT
     6121 TGTCCAGAAA ATTGATTAAC GGTATCAGAG ACAAGCAATC CGGTAAGACC ATTTTGGACT
     6181 TTTTGAAGTC TGATGGTTTC GCTAACAGAA ACTTCATGCA ATTAATCCAC GACGATTCCT
     6241 TGACTTTCAA AGAAGATATA CAAAAGGCCC AAGTCTCTGG TCAAGGTGAT TCTTTACATG
     6301 AACATATCGC TAACTTGGCT GGTTCTCCAG CTATTAAGAA GGGTATTTTA CAAACCGTTA
     6361 AGGTCGTTGA CGAATTGGTC AAAGTTATGG GTAGACATAA GCCAGAAAAC ATCGTTATCG
     6421 AAATGGCTAG AGAAAATCAA ACCACCCAAA AGGGTCAAAA GAACTCCAGA GAAAGAATGA
     6481 AGAGAATCGA AGAAGGTATC AAAGAATTGG GTTCCCAAAT TTTGAAAGAA CACCCAGTTG
     6541 AAAACACCCA ATTACAAAAC GAAAAGTTGT ACTTGTACTA CTTGCAAAAC GGTAGAGATA
     6601 TGTACGTTGA CCAAGAATTG GACATCAACA GATTGTCTGA TTACGATGTT GACGCTATCG
     6661 TTCCACAATC TTTTTTGAAG GATGACTCCA TTGACAACAA GGTCTTGACT AGATCCGATA
     6721 AGAATAGAGG TAAGTCCGAT AACGTTCCAT CTGAAGAAGT CGTTAAGAAA ATGAAGAACT
     6781 ATTGGAGACA ATTATTGAAC GCCAAGTTGA TCACCCAAAG AAAGTTTGAC AATTTGACCA
     6841 AGGCTGAAAG AGGTGGTTTG TCTGAATTGG ATAAGGCAGG TTTCATCAAA AGACAATTAG
     6901 TAGAAACCAG ACAAATCACC AAGCACGTTG CTCAAATTTT GGATAGTAGA ATGAACACTA
     6961 AGTACGACGA AAACGACAAA TTGATCAGAG AAGTTAAGGT CATTACCTTG AAGTCCAAGT
     7021 TGGTTTCCGA TTTCAGAAAG GACTTCCAAT TCTACAAGGT CAGAGAAATC AACAACTACC
     7081 ATCATGCACA TGATGCTTAC TTGAATGCTG TTGTTGGTAC TGCCTTGATT AAGAAGTATC
     7141 CAAAGTTGGA ATCCGAATTT GTCTACGGTG ATTACAAGGT TTACGACGTT AGAAAGATGA
     7201 TCGCCAAGTC CGAACAAGAA ATTGGTAAAG CTACTGCCAA ATACTTCTTC TACTCCAATA
     7261 TTATGAATTT CTTTAAGACC GAAATCACTT TGGCCAACGG TGAAATTAGA AAAAGACCAT
     7321 TGATTGAAAC TAATGGTGAA ACAGGTGAAA TCGTTTGGGA TAAGGGTAGA GATTTTGCCA
     7381 CTGTTAGAAA GGTATTGTCC ATGCCACAAG TAAACATCGT CAAAAAGACC GAAGTTCAAA
     7441 CTGGTGGTTT CTCCAAAGAA TCCATTTTGC CTAAGAGAAA CTCCGATAAG TTGATCGCTA
     7501 GAAAAAAAGA CTGGGACCCA AAAAAGTACG GTGGTTTTGA TTCTCCAACT GTTGCTTACT
     7561 CTGTTTTGGT TGTTGCTAAG GTCGAAAAGG GTAAGAGTAA GAAGTTGAAG TCCGTCAAAG
     7621 AATTATTAGG TATCACTATC ATGGAAAGAT CCTCATTCGA AAAGAATCCT ATCGACTTTT
     7681 TGGAAGCCAA GGGTTACAAA GAAGTCAAGA AGGACTTGAT CATTAAGTTG CCAAAGTACA
     7741 GTTTGTTCGA ATTGGAAAAT GGTAGAAAGA GAATGTTGGC TTCTGCCGGT GAATTACAAA
     7801 AGGGTAATGA ATTGGCTTTG CCATCCAAGT ACGTTAATTT CTTATACTTG GCCTCCCACT
     7861 ACGAAAAATT GAAAGGTTCT CCTGAAGATA ACGAACAAAA GCAATTATTT GTCGAACAAC
     7921 ACAAGCACTA CTTGGACGAA ATCATTGAAC AAATTTCCGA ATTTTCCAAA AGAGTCATTT
     7981 TGGCTGACGC CAATTTGGAC AAAGTTTTGT CAGCTTACAA CAAGCACAGA GATAAGCCAA
     8041 TTAGAGAACA AGCTGAAAAC ATCATTCACT TGTTCACTTT GACTAACTTG GGTGCTCCAG
     8101 CTGCTTTTAA GTATTTCGAT ACCACTATCG ACAGAAAGAG ATACACCTCT ACCAAAGAAG
     8161 TTTTGGACGC TACTTTGATC CACCAATCTA TTACTGGTTT GTACGAAACT AGAATCGACT
     8221 TGTCTCAATT AGGTGGTGAT TAACTCGAGT CATGTAATTA GTTATGTCAC GCTTACATTC
     8281 ACGCCCTCCC CCCACATCCG CTCTAACCGA AAAGGAAGGA GTTAGACAAC CTGAAGTCTA
     8341 GGTCCCTATT TATTTTTTTA TAGTTATGTT AGTATTAAGA ACGTTATTTA TATTTCAAAT
     8401 TTTTCTTTTT TTTCTGTACA GACGCGTGTA CGCATGTAAC ATTATACTGA AAACCTTGCT
     8461 TGAGAAGGTT TTGGGACGCT CGAAGGCTTT AATTTGCGGC CGgtacccaa ttcgccctat
     8521 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     8581 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     8641 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     8701 cgcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     8761 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     8821 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     8881 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     8941 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     9001 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     9061 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     9121 tttaacgcga attttaacaa aatattaacg tttacaattt cctgatgcgg tattttctcc
     9181 ttacgcatct gtgcggtatt tcacaccgca taggcaagtg cacaaacaat acttaaataa
     9241 atactactca gtaataacct atttcttagc atttttgacg aaatttgcta ttttgttaga
     9301 gtcttttaca ccatttgtct ccacacctcc gcttacatca acaccaataa cgccatttaa
     9361 tctaagcgca tcaccaacat tttctggcgt cagtccacca gctaacataa aatgtaagct
     9421 ttcggggctc tcttgccttc caacccagtc agaaatcgag ttccaatcca aaagttcacc
     9481 tgtcccacct gcttctgaat caaacaaggg aataaacgaa tgaggtttct gtgaagctgc
     9541 actgagtagt atgttgcagt cttttggaaa tacgagtctt ttaataactg gcaaaccgag
     9601 gaactcttgg tattcttgcc acgactcatc tccatgcagt tggacgatat caatgccgta
     9661 atcattgacc agagccaaaa catcctcctt aggttgatta cgaaacacgc caaccaagta
     9721 tttcggagtg cctgaactat ttttatatgc ttttacaaga cttgaaattt tccttgcaat
     9781 aaccgggtca attgttctct ttctattggg cacacatata atacccagca agtcagcatc
     9841 ggaatctaga gcacattctg cggcctctgt gctctgcaag ccgcaaactt tcaccaatgg
     9901 accagaacta cctgtgaaat taataacaga catactccaa gctgcctttg tgtgcttaat
     9961 cacgtatact cacgtgctca atagtcacca atgccctccc tcttggccct ctccttttct
    10021 tttttcgacc gaattaattc ttaatcggca aaaaaagaaa agctccggat caagattgta
    10081 cgtaaggtga caagctattt ttcaataaag aatatcttcc actactgcca tctggcgtca
    10141 taactgcaaa gtacacatat attacgatgc tgtctattaa atgcttccta tattatatat
    10201 atagtaatgt cgtttatgg
//