LOCUS       pPH3                    9542 bp ds-DNA     circular     21-JAN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             3240..7379
                     /label="Cas9-D10A"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        2795..3221
                     /label="GAL1 promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     terminator      7399..7638
                     /label="CYC1 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     gene            complement(8355..9177)
                     /label="TRP1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(7657..7677)
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     2758..2777
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     2720..2740
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      7847..8302
                     /label="M13 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     mutation        3267..3269
                     /label="D10A"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        2757..2775
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      complement(73..583)
                     /label="CEN-ARS pRS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      7864..8170
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_binding    2692..2714
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     NLS             7356..7376
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     complement(7686..7703)
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      1670..2352
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    913..1572
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          3387..3405
                     /label="Cas9SeqPrimer1"
                     /note="sequence: GCCTTGTTGTTCGATTCTG"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          2689..2711
                     /label="newLAC-R"
                     /note="sequence: CCACATGTGGAATTGTGAGCGGATAACA"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(7657..7676)
                     /label="T7"
                     /note="sequence: TAATACGACTCACTATAGGG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(1537..1558)
                     /label="ampR Bg1 rev"
                     /note="sequence: gtgaggcacctatctcagcgat"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          1173..1194
                     /label="ampR Bg1 fwd"
                     /note="sequence: gggggatcatgtaactcgcctt"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(7408..7426)
                     /label="CYC1Reverse"
                     /note="sequence: GCGTGAATGTAAGCGTGAC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          3233..3269
                     /label="SpeI_pTPGI_FWD"
                     /note="sequence: CTCAACTAGTATGGATAAGAAATACTCTATTGGTTTGGCT"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          7386..7413
                     /label="p415-GalL FWD2"
                     /note="sequence: CTTGTCTCAATTAGGTGGTGATTAACTCGAGTCATGTAATTAGTTATGTCACG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(3211..3235)
                     /label="p415-GalL REV2"
                     /note="sequence: TCTTGTCATCGTCATCCTTGTAATCGTTCTAGAATCCGGGGTTTTTTCTC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(3457..3489)
                     /label="SbfI-pTPG1-REV"
                     /note="sequence: TAACCTGCAGGTAGCAGATTCTGTTCTTTCTTCTGG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(7686..7703)
                     /label="M13F"
                     /note="sequence: TGTAAAACGACGGCCAGT"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          7379..7408
                     /label="p415-GalL FWD"
                     /note="sequence: CTTGTCTCAATTAGGTGGTGATTAAATCTCTTCTCGAGTCATGTAATTAGTTATG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(7703..7725)
                     /label="M13F43"
                     /note="sequence: AGGGTTTTCCCAGTCACGACGTT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(7709..7734)
                     /label="c-klURA3"
                     /note="sequence: cacggcgcgcctagcagcggTAACGCCAGGGTTTTCCCAGTCAC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          8220..8237
                     /label="pEGFP-C1-2800-R"
                     /note="sequence: GATTTCGGCCTATTGGTT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          2763..2787
                     /label="d-klURA3"
                     /note="sequence: gtcagcggccgcatccctgcCCTCACTAAAGGGAACAAAAGCTG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(9438..9458)
                     /label="pexF"
                     /note="sequence: GGAGCAGACAAGCCCGTCAGG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          2757..2776
                     /label="T3"
                     /note="sequence: AATTAACCCTCACTAAAGGG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          3243..3267
                     /label="pTPGI_dCas9 FWD"
                     /note="sequence: CTCAGAGTCCGCCACACCCGAAAGTGATAAGAAATACTCTATTGGTTTGG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2720..2737
                     /label="M13R"
                     /note="sequence: CAGGAAACAGCTATGACC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          2700..2723
                     /label="M13R49"
                     /note="sequence: GAGCGGATAACAATTTCACACAGG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2653..2678
                     /label="pexR"
                     /note="sequence: CAGGCTTTACACTTTATGCTTCCGGC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          9496..9514
                     /label="pGexR"
                     /note="sequence: GAGCTGCATGTGTCAGAGG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          2434..2453
                     /label="VR"
                     /note="sequence: ATTACCGCCTTTGAGTGAGC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(join(9542..9542,1..21))
                     /label="pQEfPR"
                     /note="sequence: GTATCACGAGGCCCTTTCGTCT"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     2761 aaccctcact aaagggaaca aaagctggAG CTCGTGACAG CCCTCCGAAG GAAGACTCTC
     2821 CTCCGTGCGT CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC
     2881 ACTGCTCCGA ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT
     2941 TGGCAGTAAC CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT
     3001 GATAATGCGA TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT
     3061 TTTGATCTAT TAACGGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT
     3121 CAACATTTTC GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT
     3181 GTTAATATAC CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATTCT AGAACTAGTA
     3241 TGGATAAGAA ATACTCTATT GGTTTGGCTA TCGGTACAAA CTCTGTTGGT TGGGCTGTTA
     3301 TTACTGATGA ATACAAGGTT CCATCCAAGA AGTTCAAGGT TTTGGGTAAC ACTGATAGAC
     3361 ACTCCATCAA AAAGAACTTG ATTGGTGCCT TGTTGTTCGA TTCTGGTGAA ACTGCTGAAG
     3421 CTACTAGATT GAAAAGAACC GCTAGAAGAA GATACACCAG AAGAAAGAAC AGAATCTGCT
     3481 ACCTGCAGGA GATCTTTAGT AATGAGATGG CTAAGGTGGA TGACTCTTTC TTCCATAGGC
     3541 TGGAGGAGTC CTTTTTGGTG GAGGAGGATA AAAAGCACGA GCGCCACCCA ATCTTTGGCA
     3601 ATATCGTGGA CGAGGTGGCG TACCATGAAA AGTACCCAAC CATATATCAT CTGAGGAAGA
     3661 AGCTTGTAGA CAGTACTGAT AAGGCTGACT TGCGGTTGAT CTATCTCGCG CTGGCGCATA
     3721 TGATCAAATT TCGGGGACAC TTCCTCATCG AGGGGGACCT GAACCCAGAC AACAGCGATG
     3781 TCGACAAACT CTTTATCCAA CTGGTTCAGA CTTACAATCA GCTTTTCGAA GAGAACCCGA
     3841 TCAACGCATC CGGAGTTGAC GCCAAAGCAA TCCTGAGCGC TAGGCTGTCC AAATCCCGGC
     3901 GGCTCGAAAA CCTCATCGCA CAGCTCCCTG GGGAGAAGAA GAACGGCCTG TTTGGTAATC
     3961 TTATCGCCCT GTCACTCGGG CTGACCCCCA ACTTTAAATC TAACTTCGAC CTGGCCGAAG
     4021 ATGCCAAGCT TCAACTGAGC AAAGACACCT ACGATGATGA TCTCGACAAT CTGCTGGCCC
     4081 AGATCGGCGA CCAGTACGCA GACCTTTTTT TGGCGGCAAA GAACCTGTCA GACGCCATTC
     4141 TGCTGAGTGA TATTCTGCGA GTGAACACGG AGATCACCAA AGCTCCGCTG AGCGCTAGTA
     4201 TGATCAAGCG CTATGATGAG CACCACCAAG ACTTGACTTT GCTGAAGGCC CTTGTCAGAC
     4261 AGCAACTGCC TGAGAAGTAC AAGGAAATTT TCTTCGATCA GTCTAAAAAT GGCTACGCCG
     4321 GATACATTGA CGGCGGAGCA AGCCAGGAGG AATTTTACAA ATTTATTAAG CCCATCTTGG
     4381 AAAAAATGGA CGGCACCGAG GAGCTGCTGG TAAAGCTTAA CAGAGAAGAT CTGTTGCGCA
     4441 AACAGCGCAC TTTCGACAAT GGAAGCATCC CCCACCAGAT TCACCTGGGC GAACTGCACG
     4501 CTATCCTCAG GCGGCAAGAG GATTTCTACC CCTTTTTGAA AGATAACAGG GAAAAGATTG
     4561 AGAAAATCCT CACATTTCGG ATACCCTACT ATGTAGGCCC CCTCGCCCGG GGAAATTCCA
     4621 GATTCGCGTG GATGACTCGC AAATCAGAAG AGACCATCAC TCCCTGGAAC TTCGAGGAAG
     4681 TCGTGGATAA GGGGGCCTCT GCCCAGTCCT TCATCGAAAG GATGACTAAC TTTGATAAAA
     4741 ATCTGCCTAA CGAAAAGGTG CTTCCTAAAC ACTCTCTGCT GTACGAGTAC TTCACAGTTT
     4801 ATAACGAGCT CACCAAGGTC AAATACGTCA CAGAAGGGAT GAGAAAGCCA GCATTCCTGT
     4861 CTGGAGAGCA GAAGAAAGCT ATCGTGGACC TCCTCTTCAA GACGAACCGG AAAGTTACCG
     4921 TGAAACAGCT CAAAGAAGAC TATTTCAAAA AGATTGAATG TTTCGACTCT GTTGAAATCA
     4981 GCGGAGTGGA GGATCGCTTC AACGCATCCC TGGGAACGTA TCACGATCTC CTGAAAATCA
     5041 TTAAAGACAA GGACTTCCTG GACAATGAGG AGAACGAGGA CATTCTTGAG GACATTGTCC
     5101 TCACCCTTAC GTTGTTTGAA GATAGGGAGA TGATTGAAGA ACGCTTGAAA ACTTACGCTC
     5161 ATCTCTTCGA CGACAAAGTC ATGAAACAGC TCAAGAGGCG CCGATATACA GGATGGGGGC
     5221 GGCTGTCAAG AAAACTGATC AATGGGATCC GAGACAAGCA GAGTGGAAAG ACAATCCTGG
     5281 ATTTTCTTAA GTCCGATGGA TTTGCCAACC GGAACTTCAT GCAGTTGATC CATGATGACT
     5341 CTCTCACCTT TAAGGAGGAC ATCCAGAAAG CACAAGTTTC TGGCCAGGGG GACAGTCTTC
     5401 ACGAGCACAT CGCTAATCTT GCAGGTAGCC CAGCTATCAA AAAGGGAATA CTGCAGACCG
     5461 TTAAGGTCGT GGATGAACTC GTCAAAGTAA TGGGAAGGCA TAAGCCCGAG AATATCGTTA
     5521 TCGAGATGGC CCGAGAGAAC CAAACTACCC AGAAGGGACA GAAGAACAGT AGGGAAAGGA
     5581 TGAAGAGGAT TGAAGAGGGT ATAAAAGAAC TGGGGTCCCA AATCCTTAAG GAACACCCAG
     5641 TTGAAAACAC CCAGCTTCAG AATGAGAAGC TCTACCTGTA CTACCTGCAG AACGGCAGGG
     5701 ACATGTACGT GGATCAGGAA CTGGACATCA ATCGGCTCTC CGACTACGAC GTGGATCATA
     5761 TCGTGCCCCA GTCTTTTCTC AAAGATGATT CTATTGATAA TAAAGTGTTG ACAAGATCCG
     5821 ATAAAAATAG AGGGAAGAGT GATAACGTCC CCTCAGAAGA AGTTGTCAAG AAAATGAAAA
     5881 ATTATTGGCG GCAGCTGCTG AACGCCAAAC TGATCACACA ACGGAAGTTC GATAATCTGA
     5941 CTAAGGCTGA ACGAGGTGGC CTGTCTGAGT TGGATAAAGC CGGCTTCATC AAAAGGCAGC
     6001 TTGTTGAGAC ACGCCAGATC ACCAAGCACG TGGCCCAAAT TCTCGATTCA CGCATGAACA
     6061 CCAAGTACGA TGAAAATGAC AAACTGATTC GAGAGGTGAA AGTTATTACT CTGAAGTCTA
     6121 AGCTGGTCTC AGATTTCAGA AAGGACTTTC AGTTTTATAA GGTGAGAGAG ATCAACAATT
     6181 ACCACCATGC GCATGATGCC TACCTGAATG CAGTGGTAGG CACTGCACTT ATCAAAAAAT
     6241 ATCCCAAGCT TGAATCTGAA TTTGTTTACG GAGACTATAA AGTGTACGAT GTTAGGAAAA
     6301 TGATCGCAAA GTCTGAGCAG GAAATAGGCA AGGCCACCGC TAAGTACTTC TTTTACAGCA
     6361 ATATTATGAA TTTTTTCAAG ACCGAGATTA CACTGGCCAA TGGAGAGATT CGGAAGCGAC
     6421 CACTTATCGA AACAAACGGA GAAACAGGAG AAATCGTGTG GGACAAGGGT AGGGATTTCG
     6481 CGACAGTCCG GAAGGTCCTG TCCATGCCGC AGGTGAACAT CGTTAAAAAG ACCGAAGTAC
     6541 AGACCGGAGG CTTCTCCAAG GAAAGTATCC TCCCGAAAAG GAACAGCGAC AAGCTGATCG
     6601 CACGCAAAAA AGATTGGGAC CCCAAGAAAT ACGGCGGATT CGATTCTCCT ACAGTCGCTT
     6661 ACAGTGTACT GGTTGTGGCC AAAGTGGAGA AAGGGAAGTC TAAAAAACTC AAAAGCGTCA
     6721 AGGAACTGCT GGGCATCACA ATCATGGAGC GATCAAGCTT CGAAAAAAAC CCCATCGACT
     6781 TTCTCGAGGC GAAAGGATAT AAAGAGGTCA AAAAAGACCT CATCATTAAG CTTCCCAAGT
     6841 ACTCTCTCTT TGAGCTTGAA AACGGCCGGA AACGAATGCT CGCTAGTGCG GGCGAGCTGC
     6901 AGAAAGGTAA CGAGCTGGCA CTGCCCTCTA AATACGTTAA TTTCTTGTAT CTGGCCAGCC
     6961 ACTATGAAAA GCTCAAAGGG TCTCCCGAAG ATAATGAGCA GAAGCAGCTG TTCGTGGAAC
     7021 AACACAAACA CTACCTTGAT GAGATCATCG AGCAAATAAG CGAATTCTCC AAAAGAGTGA
     7081 TCCTCGCCGA CGCTAACCTC GATAAGGTGC TTTCTGCTTA CAATAAGCAC AGGGATAAGC
     7141 CCATCAGGGA GCAGGCAGAA AACATTATCC ACTTGTTTAC TCTGACCAAC TTGGGCGCGC
     7201 CTGCAGCCTT CAAGTACTTC GACACCACCA TAGACAGAAA GCGGTACACC TCTACAAAGG
     7261 AGGTCCTGGA CGCCACACTG ATTCATCAGT CAATTACGGG GCTCTATGAA ACAAGAATCG
     7321 ACCTCTCTCA GCTCGGTGGA GACAGCAGGG CTGACCCCAA GAAGAAGAGG AAGGTGTGAT
     7381 CTCTTCTCGA GTCATGTAAT TAGTTATGTC ACGCTTACAT TCACGCCCTC CCCCCACATC
     7441 CGCTCTAACC GAAAAGGAAG GAGTTAGACA ACCTGAAGTC TAGGTCCCTA TTTATTTTTT
     7501 TATAGTTATG TTAGTATTAA GAACGTTATT TATATTTCAA ATTTTTCTTT TTTTTCTGTA
     7561 CAGACGCGTG TACGCATGTA ACATTATACT GAAAACCTTG CTTGAGAAGG TTTTGGGACG
     7621 CTCGAAGGCT TTAATTTGCG GCCGgtaccc aattcgccct atagtgagtc gtattacgcg
     7681 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     7741 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     7801 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcgacgc gccctgtagc
     7861 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     7921 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     7981 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     8041 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     8101 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     8161 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     8221 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     8281 aaaatattaa cgtttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta
     8341 tttcacaccg cataggcaag tgcacaaaca atacttaaat aaatactact cagtaataac
     8401 ctatttctta gcatttttga cgaaatttgc tattttgtta gagtctttta caccatttgt
     8461 ctccacacct ccgcttacat caacaccaat aacgccattt aatctaagcg catcaccaac
     8521 attttctggc gtcagtccac cagctaacat aaaatgtaag ctttcggggc tctcttgcct
     8581 tccaacccag tcagaaatcg agttccaatc caaaagttca cctgtcccac ctgcttctga
     8641 atcaaacaag ggaataaacg aatgaggttt ctgtgaagct gcactgagta gtatgttgca
     8701 gtcttttgga aatacgagtc ttttaataac tggcaaaccg aggaactctt ggtattcttg
     8761 ccacgactca tctccatgca gttggacgat atcaatgccg taatcattga ccagagccaa
     8821 aacatcctcc ttaggttgat tacgaaacac gccaaccaag tatttcggag tgcctgaact
     8881 atttttatat gcttttacaa gacttgaaat tttccttgca ataaccgggt caattgttct
     8941 ctttctattg ggcacacata taatacccag caagtcagca tcggaatcta gagcacattc
     9001 tgcggcctct gtgctctgca agccgcaaac tttcaccaat ggaccagaac tacctgtgaa
     9061 attaataaca gacatactcc aagctgcctt tgtgtgctta atcacgtata ctcacgtgct
     9121 caatagtcac caatgccctc cctcttggcc ctctcctttt cttttttcga ccgaattaat
     9181 tcttaatcgg caaaaaaaga aaagctccgg atcaagattg tacgtaaggt gacaagctat
     9241 ttttcaataa agaatatctt ccactactgc catctggcgt cataactgca aagtacacat
     9301 atattacgat gctgtctatt aaatgcttcc tatattatat atatagtaat gtcgtttatg
     9361 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     9421 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     9481 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     9541 ga
//