LOCUS       Exported                7226 bp ds-DNA     circular SYN 27-JAN-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 27, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          5825..5898
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     misc_feature    57..645
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(528..539)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 527"
                     /translation="IEGR"
     primer_bind     complement(648..664)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     LTR             1174..1354
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     rep_origin      complement(1416..2004)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(2175..3035)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 2175 .. 2966 / #ccffcc
                        2: 2967 .. 3035 / #ccffcc / signal sequence
                     Cleavage site after base 2966"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3036..3140)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     enhancer        3405..3784
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        3786..3984
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             4002..4182
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    4229..4354
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    4853..5086
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     CDS             5271..5315
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5613..5730
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     enhancer        5900..6203
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        6204..6407
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6438..7145
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             7152..7193
                     /codon_start=1
                     /label=CAAX
                     /note="color: #993366"
                     /translation="KKKKKKSKTKCVIM"
ORIGIN
        1 gtacaaacgt tgttgtttgg ggttgaatta ctcttctaga ccgcggcgcg cccgataatc
       61 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
      121 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
      181 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
      241 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
      301 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
      361 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
      421 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
      481 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
      541 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
      601 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
      661 ctcgatcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
      721 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
      781 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
      841 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
      901 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
      961 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
     1021 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
     1081 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
     1141 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
     1201 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     1261 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     1321 agaccctttt agtcagtgtg gaaaatctct agcagcatgt gagcaaaagg ccagcaaaag
     1381 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     1441 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1501 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1561 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1621 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1681 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1741 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1801 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     1861 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1921 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1981 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2041 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     2101 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     2161 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     2221 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     2281 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     2341 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     2401 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     2461 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     2521 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     2581 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     2641 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     2701 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     2761 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     2821 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     2881 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     2941 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     3001 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     3061 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     3121 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgactc gacggatcgg
     3181 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     3241 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     3301 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     3361 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
     3421 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     3481 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     3541 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     3601 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     3661 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     3721 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     3781 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     3841 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     3901 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     3961 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc
     4021 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     4081 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     4141 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     4201 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     4261 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     4321 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     4381 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     4441 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     4501 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     4561 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     4621 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     4681 cgcacagcaa gcggccggcc gcgctgatct tcagacctgg aggaggagat atgagggaca
     4741 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     4801 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     4861 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     4921 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     4981 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     5041 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     5101 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     5161 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     5221 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     5281 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     5341 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     5401 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     5461 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     5521 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     5581 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     5641 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     5701 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     5761 gtttggttag taccgggccc gggtcgacta gtgatcagtg tgagggagtg taaagctggt
     5821 ttgaacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     5881 gcccatatat ggagttcctc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     5941 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     6001 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     6061 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     6121 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     6181 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     6241 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     6301 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     6361 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcta actcgaacgc
     6421 tagctgtgcg atcgtttatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     6481 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     6541 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     6601 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     6661 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     6721 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     6781 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     6841 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     6901 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     6961 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     7021 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     7081 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     7141 acaaggaatt caagaaaaag aagaagaagt caaaaaccaa gtgtgttatc atgtaaaaac
     7201 gttgttgttt ggggttgaat tactct
//
