LOCUS       Exported                7657 bp ds-DNA     circular SYN 07-JAN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Bernard Becker Medical Library
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 26, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7657
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    607..628
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        643..673
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    681..697
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     705..721
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        742..760
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             808..837
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             841..870
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             874..903
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1435..1464
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1471..1500
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1513..1542
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1549..1578
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1792..1821
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1825..1854
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1858..1887
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     2323..2339
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2348..2381
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    5458..5491
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        complement(5536..5554)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5561..5577)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5719..6174
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6200..6304
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6305..7165
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
      601 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
      661 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
      721 ccatgattac gccaagctcg aaattaaccc tcactaaagg gaacaaaagc tggtaccgct
      781 agcaagggct cgggctcgac ccagctggag caaaaactga tatcggagga agacttggca
      841 gagcagaaac tgatttcgga agaggatctt gccgaacaaa aactgatcag tgaggaagac
      901 cttgccggag tgtcgaaggg tgaagagctg ttcaccggcg tggtgcccat cctcgtcgag
      961 ctggacggag atgtcaatgg acataaattc tccgtacggg gagagggcga gggcgacgca
     1021 accaacggta aactgaccct gaagttcatt tgcaccacgg gtaaactgcc cgtaccctgg
     1081 cccaccctgg tcacaacctt gggtggcgga gttcagtgtt tttcgcggta tccagatcac
     1141 atgaagcagc atgacttttt caagagcgct atgcccgagg gttacgttca agagaggacc
     1201 atcagtttca aggacgacgg cacctacaag acacgggctg aggtgaaatt cgagggtgat
     1261 acactcgtga atcgtatcga gctgaagggc atagatttta aggaggatgg caatatcctg
     1321 ggccacaagt tggagtataa cttcaacagt cacaatgtct atataactgc cgataagcag
     1381 aagaatggca ttaaagccaa tttcaaaatt cgtcacaatg tcgaaggcgg cgcagaacag
     1441 aagctcatca gtgaagagga tctggccgca gagcagaaac tcattagcga ggaggacttg
     1501 ggcggaggcg gcgagcagaa gctgatatcg gaggaagacc tggcggccga gcaaaagctc
     1561 atctcggagg aggatctcgc gggaggagat ggaagtgtcc agttggcgga tcactaccag
     1621 cagaatacgc caattggtga cggccccgtc cttctgccgg ataaccacta cctctccacc
     1681 cagtcggtgc tgagcaagga cccgaatgaa aaacgtgacc acatggttct tcttgagttc
     1741 gtcacggctg cgggcatcac gttgggcatg gacgaactgt acaagggcgc tgaacagaaa
     1801 cttatctccg aggaggatct ggcggaacag aagctgatca gcgaagagga cctggcggag
     1861 cagaaactta tcagcgaaga ggatctgtaa cccgggcata tgtagaaaag ttgtaacgtt
     1921 agtaaacgta acacacgggt ataatagcca tttaatggcg ccgtatattt ttcactcccc
     1981 ctctccgata ggaggaagaa agacgatccg tctgcgaatg tatattacat cgaccggcca
     2041 tagtacgaga agtggtgccg gtattgttct gtccgggggg tgtaccatcc tattttttgt
     2101 atatgtcgtt cttgtcgggg agcaacagcc catcttccgg ctattgtctc gtatcagttg
     2161 tgaaacagac tgcgttttgc cgaaaatgag tcctatgtcc gatggctatt tgtaccgctt
     2221 cgtaccattt cgcttgtcag ctgtaatcaa ccgaattcat ttggcgagac atgattccga
     2281 ttaaattctt tactgcgcgt gctcggatcc actagttcta ctcgaggtcg acggtatcga
     2341 taagcttata acttcgtata gcatacatta tacgaagtta tcagcacgaa accttgcatt
     2401 caaacccgcc cgcggaagat ccgatcttgc tgctgttcgc agtcccagta gcgtcctgtc
     2461 ggccgcgccg tctctgttgg tgggcagccg ctacacctgt tatctgactg ccgtgcgcga
     2521 aaatgacgcc atttttggga aaatcgggga acttcattct ttaaaagtat gcggaggttt
     2581 cctttttctt ctgttcgttt ctttttctcg ggtttgataa ccgtgttcga tgtaagcact
     2641 ttccgtctct cctccgtgct ttgttcgaca tcgagaccag gtgtgcagat ccttcgcttg
     2701 tcgatccgga gacgcgtgtc tcgtagaacc ttttcatttt accacacggc agtgcggagc
     2761 actgctctga gtgcagcagg gacgggtgaa gtttcgcttt agtagtgcgt ttctgctcta
     2821 cggggcgttg tcagatccag caaaatgcag aaaccggtgt gtctggtcgt cgcgatgacc
     2881 cccaagaggg gcatcggcat caacaacggc ctcccgtggc cccacttgac cacagatttc
     2941 aaacactttc gtcgtgtgac aaaaacgacg cccgaagaag ccagtcgcct gaacgggtgg
     3001 cttcccagga aatttgcaaa gacgggcgac tctggacttc cctctccatc agtcggcaag
     3061 agattcaacg ccgttgtcat gggacggaaa aactgggaaa gcatgcctcg aaagtttaga
     3121 cccctcgtgg acagattgaa catcgtcgtt tcctcttccc tcaaagaaga agacattgcg
     3181 gcggagaagc ctcaagctga aggccagcag cgcgtccgag tctgtgcttc actcccagca
     3241 gctctcagcc ttctggagga agagtacaag gattctgtcg accagatttt tgtcgtggga
     3301 ggagcgggac tgtacgaggc agcgctgtct ctgggcgttg cctctcacct gtacatcact
     3361 cgcgtagccc gcgagtttcc gtgcgacgtt ttcttccctg cgttccccgg agatgacatt
     3421 ctttcaaaca aatcaactgc tgcgcaggct gcagctcctg ccgagtctgt gttcgttccc
     3481 ttttgtccgg agctcggaag agagaaggac aatgaagcga cgtatcgacc catcttcatt
     3541 tccaagacct tctcagacaa cggcgtaccc tacgactttg tggttctcga gaagagaagg
     3601 aagactgacg acgcagccac tgcggaaccg agcaacgcaa tgagctcctt gacgtccacg
     3661 agggagacaa ctcccgtgca cgggttgcag gctccttctt cggccgcagc cattgccccg
     3721 gtgttggcgt ggatggacga agaagaccgg aaaaaacgcg agcaaaagga actgattcgg
     3781 gccgttccgc atgttcactt tagaggccat gaagaattcc agtaccttga tctcattgcc
     3841 gacattatta acaatggaag gacaatggat gaccgaacgg gcgttggtgt catctccaaa
     3901 ttcggctgca ctatgcgcta ctcgctggat caggcctttc cacttctcac cacaaagcgt
     3961 gtgttctgga aaggggtcct cgaagagttg ctgtggttca ttcgcggcga cacgaacgca
     4021 aaccatcttt ctgagaaggg cgtgaagatc tgggacaaga atgtgacacg cgagttcctc
     4081 gattcgcgca atctccccca ccgagaggtc ggagacatcg gcccgggcta cggcttccag
     4141 tggagacact tcggcgcggc atacaaagac atgcacacag actacacagg gcagggcgtc
     4201 gaccagctga agaatgtgat ccagatgctg agaacgaatc caacagatcg tcgcatgctc
     4261 atgactgcct ggaatcctgc agcgctggac gaaatggcgc tgccgccttg tcacttgttg
     4321 tgccagttct acgtgaacga ccagaaggag ctgtcgtgca tcatgtatca gcggtcgtgc
     4381 gatgtcggcc tcggcgtccc cttcaacatc gcttcctatt cgcttttgac gctcatggtt
     4441 gcacacgtct gcaacctaaa acctaaggag ttcattcact tcatggggaa cacgcatgtc
     4501 tacacgaacc atgtcgaggc tttaaaagag cagctgcgga gagaaccgag accgttcccc
     4561 attgtgaaca tcctcaacaa ggaacgcatc aaggaaatcg acgatttcac cgccgaggat
     4621 tttgaggtcg tgggctacgt cccgcacgga cgaatccaga tggagatggc tgtctaaacc
     4681 ctgcatagcc cacagaagct gcccgtctct cgttttcctc tcttttcgga gggatcaggg
     4741 agagtgcctc ggatcggaga gagctgacga gggggtgcca gagacccctg tgtcctttat
     4801 cgaagaaaag ggatgactct tcatgtggca tttcacacag tctcacctcg ccttgttttc
     4861 tttttgtcaa tcagaacgaa agcgagttgc gggtgacgca gatgtgcgtg tatccactcg
     4921 tgaatgcgtt atcgttctgt atgccgctag agtgctggac tgttgctgtc tgcccacgac
     4981 agcagacaac tttccttcta tgcacttgca ggatggtgca gcgcaaacga cggagagaaa
     5041 ggagcaccct ctcagtttcc ctacgatgtg ctgtcagttt cgactcttca ccgcgaacga
     5101 ttggcgatac gtctctgttg acttgttagg ctccgaccac gaagctccct taactagata
     5161 agccgcgaca cctaagtgta caccatttgc agatcgataa tctgcgaccg ctgaatccgt
     5221 ccagatcagt aaaaccgcac cacctaagtg taaaccttgt ttaggtcgat aaaatgctac
     5281 caacccccac ccacaatcga gccttgagcg tttctgcgca cgcgttggcc tacgtgactt
     5341 gctgatgcct gcctctggcc attcatgcca gtcagtgcgc ataaaaatgt ggacacagtc
     5401 ggttgacaag tgttctggca ggctacagtg acaccgcggt ggagggggat cattgaaata
     5461 acttcgtata gcatacatta tacgaagtta tctgcagtct agagcggccg ccaccgcggt
     5521 ggagctccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
     5581 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     5641 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     5701 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     5761 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     5821 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     5881 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     5941 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     6001 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     6061 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     6121 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     6181 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6241 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6301 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6361 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6421 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6481 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6541 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6601 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6661 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6721 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6781 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6841 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6901 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6961 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7021 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7081 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7141 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7201 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7261 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7321 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7381 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7441 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7501 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7561 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7621 atagttaccg gataaggcgc agcggtcggg ctgaacg
//
