LOCUS       Exported               11703 bp ds-DNA     circular SYN 25-JAN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 26, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11703
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     promoter        1717..1957
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    1966..1985
                     /label=YAP GRNA2
     misc_RNA        1986..2061
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2109..2226
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2295..2506
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2526..2535
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2535..2558
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2565..2585
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2610..6710
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6711..6758
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6774..6830
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6831..7544
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    7560..8148
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8031..8042)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8220..8453
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8531..8652
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8692..8827
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(8848..8866)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8876..8892)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      9034..9489
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9515..9619
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9620..10480
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10651..11239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11527..11548
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11563..11593
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11601..11617
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11625..11641
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11662..11680
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagagagg gcctatttcc catgattcct
     1741 tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta
     1801 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     1861 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     1921 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccccgcc gtccgtgtct
     1981 ccggcgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
     2041 aaagtggcac cgagtcggtg cttttttgaa ttctagatct tgagacaaat ggcagtattc
     2101 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2161 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2221 aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct cgagtggctc
     2281 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     2341 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     2401 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     2461 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtgt cgtgacgcgg
     2521 gatccgccac catggattac aaagacgatg acgataagat ggccccaaag aagaagcgga
     2581 aggtcggtat ccacggagtc ccagcagccg acaagaagta cagcatcggc ctggacatcg
     2641 gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat
     2701 tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc
     2761 tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat
     2821 acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca
     2881 aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga
     2941 agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt
     3001 accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc
     3061 ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg
     3121 gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct
     3181 acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc
     3241 tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag ctgcccggcg
     3301 agaagaagaa tggcctgttc ggaaacctga ttgccctgag cctgggcctg acccccaact
     3361 tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg
     3421 acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg
     3481 ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga
     3541 tcaccaaggc ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc
     3601 tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct
     3661 tcgaccagag caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt
     3721 tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga
     3781 agctgaacag agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc
     3841 accagatcca cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat
     3901 tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg
     3961 tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa
     4021 ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca
     4081 tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca
     4141 gcctgctgta cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg
     4201 agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc
     4261 tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa
     4321 tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg
     4381 gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa
     4441 acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga
     4501 tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga
     4561 agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg
     4621 acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa
     4681 acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc
     4741 aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg
     4801 ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg
     4861 gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga
     4921 agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg
     4981 gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt
     5041 acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc
     5101 ggctgtccga ctacgatgtg gaccatatcg tgcctcagag ctttctgaag gacgactcca
     5161 tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct
     5221 ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga
     5281 ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg
     5341 ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg
     5401 cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg
     5461 aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt
     5521 tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg
     5581 tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg
     5641 actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg
     5701 ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc
     5761 tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga
     5821 tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag
     5881 tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc
     5941 ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg
     6001 gcggcttcga cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg
     6061 gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa
     6121 gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa
     6181 aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga
     6241 gaatgctggc ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat
     6301 atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata
     6361 atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc
     6421 agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt
     6481 ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc
     6541 tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg
     6601 accggaagag gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca
     6661 tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggcgac aagcgtcctg
     6721 ctgctactaa gaaagctggt caagctaaga aaaagaaagc tagcggcagc ggcgccacca
     6781 acttcagcct gctgaagcag gccggcgacg tggaggagaa ccccggcccc atggtgtcta
     6841 agggcgaaga gctgattaag gagaacatgc acatgaagct gtatatggag ggcaccgtga
     6901 acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
     6961 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
     7021 ccagcttcat gtacggcagc agaaccttca tcaaccacac ccagggcatc cccgacttct
     7081 ttaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
     7141 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
     7201 agatcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acactcggct
     7261 gggaggccaa caccgagatg ctgtaccccg ctgacggcgg cctggaaggc agaagcgaca
     7321 tggccctgaa gctcgtgggc gggggccacc tgatctgcaa cttcaagacc acatacagat
     7381 ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac cacagactgg
     7441 aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgaggtg gctgtggcca
     7501 gatactgcga cctccctagc aaactggggc acaaacttaa ttgaacgcgt taagtcgaca
     7561 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     7621 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     7681 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     7741 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     7801 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     7861 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     7921 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     7981 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     8041 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     8101 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag
     8161 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     8221 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     8281 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8341 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8401 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtacgta
     8461 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     8521 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     8581 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8641 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     8701 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     8761 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     8821 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     8881 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     8941 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     9001 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     9061 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     9121 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     9181 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     9241 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     9301 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     9361 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     9421 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     9481 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     9541 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     9601 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     9661 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     9721 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     9781 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     9841 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     9901 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     9961 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
    10021 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
    10081 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
    10141 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
    10201 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
    10261 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
    10321 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
    10381 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
    10441 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
    10501 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    10561 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    10621 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    10681 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    10741 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    10801 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    10861 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    10921 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    10981 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    11041 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    11101 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    11161 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    11221 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    11281 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    11341 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    11401 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
    11461 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
    11521 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
    11581 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
    11641 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
    11701 agc
//
