LOCUS       Exported                5781 bp ds-DNA     circular SYN 22-NOV-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 28, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     51..61
                     /label=ps211
                     /note="vwa5a 5pF1"
     promoter        175..622
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     promoter        175..622
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    559..576
                     /label=Feature 26
     primer_bind     559..576
                     /label=ps270
                     /note="hSyn1 F"
     protein_bind    662..680
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    662..680
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    698..716
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    698..716
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    805..852
                     /label=FRT
                     /note="FRT"
     primer_bind     858..878
                     /label=ps269
                     /note="F cag flex-FRT-rGFP"
     misc_feature    903..950
                     /label=F3
                     /note="F3"
     primer_bind     966..998
                     /label=ps267
                     /note="R rtta with random hex -EcorI"
     primer_bind     984..1000
                     /label=ps265
                     /note="R tTA with random hexamer-EcorI and last 24bp"
     CDS             complement(985..1731)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(1712..1737)
                     /label=ps266
                     /note="F rtta with random hexamer -SalI"
     misc_feature    complement(1742..1789)
                     /label=FRT
                     /note="FRT"
     primer_bind     complement(1778..1803)
                     /label=ps268
                     /note="R cag flex-FRT-rGFP"
     misc_feature    complement(1840..1887)
                     /label=rF3
                     /note="rF3"
     misc_feature    1908..2496
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1965..1984)
                     /label=ps125
                     /note="Ai65 PCS seq R1"
     primer_bind     complement(1983..2002)
                     /label=ps145
                     /note="Rosa26TdT (007909) Mutant Reverse (WPRE)"
     primer_bind     complement(2001..2024)
                     /label=ps126
                     /note="Ai65 PCS seq R2"
     primer_bind     complement(2075..2094)
                     /label=ps178
                     /note="P18 R CRE-dsGFP deepseq"
     primer_bind     complement(2235..2250)
                     /label=ps124
                     /note="R Ai65 MluI test"
     CDS             complement(2379..2390)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(2379..2390)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2519..2535
                     /label=ps185
                     /note="P16 F CRE-dsGFP deepseq"
     primer_bind     complement(2522..2539)
                     /label=ps169
                     /note="F Ai65 CRE-dsGFP long range PCR  2 (between FRT"
     polyA_signal    2528..3004
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     polyA_signal    2528..3004
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     2549..2559
                     /label=ps088
                     /note="5'1 R WT Gdf15r-IRES-Cre"
     primer_bind     complement(2678..2697)
                     /label=ps188
                     /note="P11 R CRE-dsGFP deepseq"
     primer_bind     2699..2718
                     /label=ps183
                     /note="P14 F CRE-dsGFP deepseq"
     primer_bind     2905..2917
                     /label=ps246
                     /note="SF1FlpO 5p Targetting 2"
     repeat_region   3044..3184
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3044..3184
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     complement(3113..3123)
                     /label=ps211
                     /note="vwa5a 5pF1"
     rep_origin      3259..3714
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      3259..3714
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3996..4100
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3996..4100
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4101..4961
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             4101..4961
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5132..5720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5132..5720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5533..5559
                     /label=-
                     /note="F PX330 PRIMER"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca
      181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
      241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
      301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
      361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
      421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
      481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
      541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
      601 cgtgtcgtgc ctgagagcgc agtcgagaac agatctctaa aacaggtaag tcccattaat
      661 ctccctatca gtgatagaga aggtctgaag agtttactcc ctatcagtga tagagattaa
      721 ttcctctact aaccttgttc atcttttctt tttttttcta caggtcctgg gtgattaaca
      781 gcttaagggc ccggatccga tatcgaagtt cctattccga agttcctatt cttcaaatag
      841 tataggaact tcgcagaatg gtagctggat tgtagctgct attagcaata tgaaacctct
      901 tagaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc gagctcggcg
      961 cgccggaatt cactagtctc gagcttaccc ggggagcatg tcaaggtcaa aatcgtcaag
     1021 agcgtcagca ggcagcatat caaggtcaaa gtcgtcaagg gcatcggctg ggagcatgtc
     1081 taagtcaaaa tcgtcaaggg cgtcggccgg cccgccgctt tcgcacttta gctgtttctc
     1141 caggccacat atgattagtt ccaggccgaa aaggaaggca ggttcggctc cctgccggtc
     1201 gaacagctca attgcttgtc tcagaagtgg gggcatagaa tcggtggtag gtgtctctct
     1261 ttcctctttt gctacttgat gctcctgttc ctccaatacg cagcccagtg taaagtggcc
     1321 cacggcggac agagcgtaca gtgcgttctc cagggagaag ccttgctgac acaggaacgc
     1381 gagctgattt tccagggttt cgtactgttt ctctgttggg cgggtgccga gatgcacttt
     1441 agccccgtcg cgatgtgaga ggagagcaca gcggaatgac ttggcgttgt tccgcagaaa
     1501 gtcttgccat gactcgcctt ccagggggca gaagtgggta tgatgcctgt ccagcatctc
     1561 gattggcagg gcatcgagca gggcccgctt gttcttcacg tgccagtaca gggtaggctg
     1621 ctcaactccc agcttttgag cgagtttcct tgtcgtcagg ccttcgatac cgactccatt
     1681 gagtaattcc agagcgccgt ttatgacttt gctcttgtcc agtctagaca tgtcgacccg
     1741 ggaagttcct atactatttg aagaatagga acttcggaat aggaacttct tgccttaacc
     1801 cagaaattat cactgttatt ctttagaatg gtgcaaagag aagttcctat actttctaga
     1861 gaataggaac ttcggaatag gaacttcgaa gtactaagct tatcgataat caacctctgg
     1921 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     1981 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     2041 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     2101 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     2161 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     2221 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     2281 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca
     2341 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     2401 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     2461 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga
     2521 gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc
     2581 cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta
     2641 ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg
     2701 gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca
     2761 atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc
     2821 cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta
     2881 gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta
     2941 cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt
     3001 ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg
     3061 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     3121 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     3181 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     3241 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3301 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
     3361 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     3421 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     3481 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     3541 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
     3601 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
     3661 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     3721 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     3781 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     3841 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     3901 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     3961 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4021 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4081 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4141 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4201 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4261 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4321 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4381 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4441 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4501 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     4561 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4621 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4681 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4741 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4801 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4861 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4921 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4981 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5041 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5101 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5161 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5221 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5281 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5341 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5401 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5461 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5521 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5581 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5641 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5701 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5761 gctggccttt tgctcacatg t
//
