LOCUS       Exported                8294 bp ds-DNA     circular UNA 27-JAN-2021
DEFINITION  A new nucleotide sequence entered manually.
ACCESSION   urn.local...7a-825eugu
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 29, 2021 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8294
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     gene            complement(1..663)
                     /label=rep
     misc_feature    157..167
                     /label=BglI
                     /note="Geneious type: restriction site"
     rep_origin      754..1571
                     /label=oriV-RK2
     misc_feature    1509..1519
                     /label=BglI
                     /note="Geneious type: restriction site"
     gene            1657..2655
                     /label=mob
     misc_feature    2134..2144
                     /label=BglI
                     /note="Geneious type: restriction site"
     misc_feature    2386..2396
                     /label=BglI
                     /note="Geneious type: restriction site"
     promoter        2775..2824
                     /label=kan promoter
     misc_feature    2913..3707
                     /label=kan
     misc_feature    complement(4006..4944)
                     /label=rhaR
     misc_feature    4850..4860
                     /label=BglI
                     /note="Geneious type: restriction site"
     misc_feature    complement(4928..5764)
                     /label=rhaS
     CDS             6059..6712
                     /label=recE tji49
     misc_feature    6698..6708
                     /label=BglI
                     /note="Geneious type: restriction site"
     CDS             6726..7745
                     /label=recT tji49
     CDS             7763..7930
                     /label=HP tji49
     terminator      7951..7978
                     /label=trpA term terminator
                     /label=trpA term
ORIGIN
        1 ctaccggcgc ggcagcgtga cccgtgtcgg cggctccaac ggctcgccat cgtccagaaa
       61 acacggctca tcgggcatcg gcaggcgctg ctgcccgcgc cgttcccatt cctccgtttc
      121 ggtcaaggct ggcaggtctg gttccatgcc cggaatgccg ggctggctgg gcggctcctc
      181 gccggggccg gtcggtagtt gctgctcgcc cggatacagg gtcgggatgc ggcgcaggtc
      241 gccatgcccc aacagcgatt cgtcctggtc gtcgtgatca accaccacgg cggcactgaa
      301 caccgacagg cgcaactggt cgcggggctg gccccacgcc acgcggtcat tgaccacgta
      361 ggccgacacg gtgccggggc cgttgagctt cacgacggag atccagcgct cggccaccaa
      421 gtccttgact gcgtattgga ccgtccgcaa agaacgtccg atgagcttgg aaagtgtctt
      481 ctggctgacc accacggcgt tctggtggcc catctgcgcc acgaggtgat gcagcagcat
      541 tgccgccgtg ggtttcctcg caataagccc ggcccacgcc tcatgcgctt tgcgttccgt
      601 ttgcacccag tgaccgggct tgttcttggc ttgaatgccg atttctctgg actgcgtggc
      661 catgcttatc tccatgcggt agggtgccgc acggttgcgg caccatgcgc aatcagctgc
      721 aacttttcgg cagcgcgaca acaattatgc gttgcgtaaa agtggcagtc aattacagat
      781 tttctttaac ctacgcaatg agctattgcg gggggtgccg caatgagctg ttgcgtaccc
      841 ccctttttta agttgttgat ttttaagtct ttcgcatttc gccctatatc tagttctttg
      901 gtgcccaaag aagggcaccc ctgcggggtt cccccacgcc ttcggcgcgg ctccccctcc
      961 ggcaaaaagt ggcccctccg gggcttgttg atcgactgcg cggccttcgg ccttgcccaa
     1021 ggtggcgctg cccccttgga acccccgcac tcgccgccgt gaggctcggg gggcaggcgg
     1081 gcgggcttcg ccttcgactg cccccactcg cataggcttg ggtcgttcca ggcgcgtcaa
     1141 ggccaagccg ctgcgcggtc gctgcgcgag ccttgacccg ccttccactt ggtgtccaac
     1201 cggcaagcga agcgcgcagg ccgcaggccg gaggcttttc cccagagaaa attaaaaaaa
     1261 ttgatggggc aaggccgcag gccgcgcagt tggagccggt gggtatgtgg tcgaaggctg
     1321 ggtagccggt gggcaatccc tgtggtcaag ctcgtgggca ggcgcagcct gtccatcagc
     1381 ttgtccagca gggttgtcca cgggccgagc gaagcgagcc agccggtggc cgctcgcggc
     1441 catcgtccac atatccacgg gctggcaagg gagcgcagcg accgcgcagg gcgaagcccg
     1501 gagagcaagc ccgtagggcg ccgcagccgc cgtaggcggt cacgactttg cgaagcaaag
     1561 tctagtgagt atactcaagc attgagtggc ccgccggagg caccgccttg cgctgccccc
     1621 gtcgagccgg ttggacacca aaagggaggg gcaggcatgg cggcatacgc gatcatgcga
     1681 tgcaagaagc tggcgaaaat gggcaacgtg gcggccagtc tcaagcacgc ctaccgcgag
     1741 cgcgagacgc ccaacgctga cgccagcagg acgccagaga acgagcactg ggcggccagc
     1801 agcaccgatg aagcgatggg ccgactgcgc gagttgctgc cagagaagcg gcgcaaggac
     1861 gctgtgttgg cggtcgagta cgtcatgacg gccagcccgg aatggtggaa gtcggccagc
     1921 caagaacagc aggcggcgtt cttcgagaag gcgcacaagt ggctggcgga caagtacggg
     1981 gcggatcgca tcgtgacggc cagcatccac cgtgacgaaa ccagcccgca catgaccgcg
     2041 ttcgtggtgc cgctgacgca ggacggcagg ctgtcggcca aggagttcat cggcaacaaa
     2101 gcgcagatga cccgcgacca gaccacgttt gcggccgctg tggccgatct agggctgcaa
     2161 cggggcatcg agggcagcaa ggcacgtcac acgcgcattc aggcgttcta cgaggccctg
     2221 gagcggccac cagtgggcca cgtcaccatc agcccgcaag cggtcgagcc acgcgcctat
     2281 gcaccgcagg gattggccga aaagctggga atctcaaagc gcgttgagac gccggaagcc
     2341 gtggccgacc ggctgacaaa agcggttcgg caggggtatg agcctgccct acaggccgcc
     2401 gcaggagcgc gtgagatgcg caagaaggcc gatcaagccc aagagacggc ccgagacctt
     2461 cgggagcgcc tgaagcccgt tctggacgcc ctggggccgt tgaatcggga tatgcaggcc
     2521 aaggccgccg cgatcatcaa ggccgtgggc gaaaagctgc tgacggaaca gcgggaagtc
     2581 cagcgccaga aacaggccca gcgccagcag gaacgcgggc gcgcacattt ccccgaaaag
     2641 tgccacctgg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa
     2701 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat
     2761 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
     2821 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat
     2881 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     2941 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     3001 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     3061 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     3121 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     3181 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     3241 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     3301 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     3361 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     3421 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     3481 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     3541 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     3601 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     3661 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     3721 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     3781 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     3841 ccagcgcggg gatctcatgc tggagttctt cgcccacccc catgggcaaa tattatacgc
     3901 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     3961 tccatgtcgg cagaatgctt aatgaattac aacagttttt atgcattaat ctttctgcga
     4021 attgagatga cgccactggc tgggcgtcat cccggtttcc cgggtaaaca ccaccgaaaa
     4081 atagttacta tcttcaaagc cacattcggt cgaaatatca ctgattaaca ggcggctatg
     4141 ctggagaaga tattgcgcat gacacactct gacctgtcgc agatattgat tgatggtcat
     4201 tccagtctgc tggcgaaatt gctgacgcaa aacgcgctca ctgcacgatg cctcatcaca
     4261 aaatttatcc agcgcaaagg gacttttcag gctagccgcc agccgggtaa tcagcttatc
     4321 cagcaacgtt tcgctggatg ttggcggcaa cgaatcactg gtgtaacgat ggcgattcag
     4381 caacatcacc aactgcccga acagcaactc agccatttcg ttagcaaacg gcacatgctg
     4441 actactttca tgctcaagct gaccgataac ctgccgcgcc tgcgccatcc ccatgctacc
     4501 taagcgccag tgtggttgcc ctgcgctggc gttaaatccc ggaatcgccc cctgccagtc
     4561 aagattcagc ttcagacgct ccgggcaata aataatattc tgcaaaacca gatcgttaac
     4621 ggaagcgtag gagtgtttat cgtcagcatg aatgtaaaag agatcgccac gggtaatgcg
     4681 ataagggcga tcgttgagta catgcaggcc attaccgcgc cagacaatca ccagctcaca
     4741 aaaatcatgt gtatgttcag caaagacatc ttgcggataa cggtcagcca cagcgactgc
     4801 ctgctggtcg ctggcaaaaa aatcatcttt gagaagtttt aactgatgcg ccaccgtggc
     4861 tacctcggcc agagaacgaa gttgattatt cgcaatatgg cgtacaaata cgttgagaag
     4921 attcgcgtta ttgcagaaag ccatcccgtc cctggcgaat atcacgcggt gaccagttaa
     4981 actctcggcg aaaaagcgtc gaaaagtggt tactgtcgct gaatccacag cgataggcga
     5041 tgtcagtaac gctggcctcg ctgtggcgta gcagatgtcg ggctttcatc agtcgcaggc
     5101 ggttcaggta tcgctgaggc gtcagtcccg tttgctgctt aagctgccga tgtagcgtac
     5161 gcagtgaaag agaaaattga tccgccacgg catcccaatt cacctcatcg gcaaaatggt
     5221 cctccagcca ggccagaagc aagttgagac gtgatgcgct gttttccagg ttctcctgca
     5281 aactgctttt acgcagcaag agcagtaatt gcataaacaa gatctcgcga ctggcggtcg
     5341 agggtaaatc attttcccct tcctgctgtt ccatctgtgc aaccagctgt cgcacctgct
     5401 gcaatacgct gtggttaacg cgccagtgag acggatactg cccatccagc tcttgtggca
     5461 gcaactgatt cagcccggcg agaaactgaa atcgatccgg cgagcgatac agcacattgg
     5521 tcagacacag attatcggta tgttcataca gatgccgatc atgatcgcgt acgaaacaga
     5581 ccgtgccacc ggtgatggta tagggctgcc cattaaacac atgaataccc gtgccatgtt
     5641 cgacaatcac aatttcatga aaatcatgat gatgttcagg aaaatccgcc tgcgggagcc
     5701 ggggttctat cgccacggac gcgttaccag acggaaaaaa atccacacta tgtaatacgg
     5761 tcatactggc ctcctgatgt cgtcaacacg gcgaaatagt aatcacgagg tcaggttctt
     5821 accttaaatt ttcgacggaa aaccacgtaa aaaacgtcga tttttcaaga tacagcgtga
     5881 attttcagga aatgcggtga gcatcacatc accacaattc agcaaattgt gaacatcatc
     5941 acgttcatct ttccctggtt gccaatggcc cattttcctg tcagtaacga gaaggtcgcg
     6001 aattcaggcg ctttttagac tggtcgtaat gaacaattct taagaaggag atatacatat
     6061 gcgaatcgtt gaatgcgcac agggtagcga cgaatggcat cgcgcgcgtg ccggcgtcat
     6121 cacggccagc atgttcgccg cggcgcgcaa gcgtctcaag gtcggcccta acaagggcga
     6181 ttacacgtcc gaggcgaaag actatgcatt ccgcctcgcg gtcgagcgca ttggcggtca
     6241 gccgctcgac gaaggattcg agacgtggca gatgaagcgc gggcacgagc tcgaacccga
     6301 ggcgcgcatg gagcacgaag cactcacagg cctgttcgtg aagcgcgccg gattcgtcgt
     6361 gaccgatgac ggactgttcg gcgcgagcgc tgacggcctg atcgaacccg atggcggcgc
     6421 cgaatacaaa tgcttcgttg cgccggacaa gctgcgcccg atgctgctcg acaacgacgc
     6481 cggcgacgtc atggaacagg cgcagggctg catgtggctt tgcggcgcga agtggtggca
     6541 cgtcggcctg tactgccccg ccctcgccgc tgctggccgc cagttcacgc tgatcaaggt
     6601 acaacgcgac gatgcattca tcgagcgcat ggaggccgat ctcgtcgagt tctcgcgtct
     6661 cgtcactgcg tacgaaacac ggctgcgcca gcacccagcc attgaggctt gaaggaggca
     6721 gctatatgag caatgccctc atcgccgtca ccaacgaaat ctacggcgtc cgtgaaaact
     6781 tcgagcgtgt cgccgtcgat cgctcgatca acttcgaccg cgaggccgga tttgcagtgc
     6841 agatcctgca aggcaatccg tacttgctcg gcgtcgccga gaaggatcgc gcttcgatga
     6901 tcgcggcggt gacgaacgtt tcggcgattg gcatcagcct caaccccgcg aagaagcagg
     6961 cgtacctcgt gccgcgtggc ggcaaggtct gtctcgacat cagctacatg gggctgctgg
     7021 atctggcgat cgcctccggt tcgatccagt ggggtcaagc cgaagtggtg tgcgaagaag
     7081 acacgttcac cctgcgcggc ttcgacaaag cgccgttgca tgacttcgca ccgttcagca
     7141 agacgcgtgg cgcagtggtc ggcgtctatg tcgtcgtcaa gaccgcgacc ggcgactacc
     7201 tgaccgacac gatgacgatc gccgaggtac aggcgatcaa ggctcgctcc gaaaccggca
     7261 agaaggacac cgggccgtgg aaaacggact tcaacgaaat ggcgaagaag accgtcatca
     7321 agcgagccta caagacgtgg ccgaagacgg atcgccttga tgcggccgtg caccacctga
     7381 acaccgatgg cggcgaaggc atcaacttcg ccagtgagcg cccgaattac tgcgacccgg
     7441 acgtgctgaa agcatgggtc gacaaggcga aggctgcgcg caccgctgaa tcgctcgcga
     7501 agatttggca ggacggtttg gctgtcatcc ggcctaccaa agacatgcag acttacgaag
     7561 tattcaagca gacggtcgcg gttcgtggcg aagaaattaa gcgccgcgcc gagcagcgga
     7621 acaatgctca ggacgtgacg ccgactgagc cagcacagcg tgagccgggc gccgacgacg
     7681 agctcgaaga agatttcagg cgccagatgg agcgcgagca agggggtgcc gatgcgaatc
     7741 gttgactcga ggaggggatc ctatgaaccc gatcacctac ctgtgcggcg cgctcgaccg
     7801 gctgttcgaa cgtagtccgg tcgcggggat tgttgttgcg atcgtgattg cggccggatg
     7861 cgtgatcgcg atcgcctatc tcaaccagga cggcgagtcc gtcaccacta tcaatgcgag
     7921 gtttgcatga ctcgaggatc ctgtacaccc agcccgccta atgagcgggc ttttttttga
     7981 acaaatgagc ttaccggttt attgactacc ggaagcagtg tgaccgtgtg cttctcaaat
     8041 gcctgaggcc agtttgctca ggctctcccc gtggaggtaa taattgacga tatgatcatt
     8101 tattctgcct cccagagcct gataaaaacg gtgaatccgt tagcgaggtg ccgccggctt
     8161 ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa
     8221 ggtatagggc ggcgaggcgg ctacagccga tagtctggaa cagcgcactt acgggttgct
     8281 gcgcaaccca agtg
//
