LOCUS       Exported                5844 bp ds-DNA     circular SYN 01-FEB-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   LC_Cheah
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 1, 2021 from SnapGene 5.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..235
                     /label=S. cerevisiae URA3 terminator
     primer_bind     complement(51..70)
                     /label=Rseq_ScURA3t
     protein_bind    260..276
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator 
                     toinhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(284..314)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    329..350
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the 
                     presenceof cAMP."
     rep_origin      complement(638..1226)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1397..2257)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2258..2362)
                     /gene="bla"
                     /label=AmpR promoter
     gene            3029..3832
                     /label=Kluyveromyces lactis URA3
     terminator      3833..3950
                     /label=K. lactis URA3 terminator
     protein_bind    3966..3999
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        4017..4684
                     /label=5' GAL10/GAL1 3' promoter
     protein_bind    4232..4349
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediatingGal4-dependent
                     induction"
     primer_bind     4601..4632
                     /label=Fseq_GAL1p
     gene            4694..5836
                     /label=deltaVP1
ORIGIN
        1 actagtagga ctatttgcaa agggaaggga tgctaaggta gagggtgaac gttacagaaa
       61 agcaggctgg gaagcatatt tgagaagatg cggccagcaa aactaaaaaa ctgtattata
      121 agtaaatgca tgtatactaa actcacaaat tagagcttca atttaattat atcagttatt
      181 acccgggaat ctcggtcgta atgatttaaa tgcatgcaag cttggcgtaa tcatggtcat
      241 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      301 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      361 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
      421 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
      481 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      541 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      601 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      661 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      721 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      781 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      841 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
      901 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
      961 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1021 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     1081 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1141 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     1201 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     1261 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     1321 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     1381 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     1441 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     1501 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     1561 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     1621 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     1681 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     1741 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     1801 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     1861 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     1921 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     1981 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     2041 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     2101 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     2161 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     2221 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     2281 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     2341 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     2401 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     2461 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     2521 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     2581 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     2641 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     2701 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     2761 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     2821 cccagtcacg acgttgtaaa acgacggcca gtgaattcat ttaaattttt ttgattcggt
     2881 aatctccgag cagaaggaag aacgaaggaa ggagcacaga cttagattgg tatatatacg
     2941 catatgtggt gttgaagaaa catgaaattg cccagtattc ttaacccaac tgcacagaac
     3001 aaaaacctgc aggaaacgaa gataaaacat gtccacaaaa tcatatacca gtagagctga
     3061 gactcatgca agtccggttg catcgaaact tttacgttta atggatgaaa agaagaccaa
     3121 tttgtgtgct tctcttgacg ttcgttcgac tgatgagcta ttgaaacttg ttgaaacgtt
     3181 gggtccatac atttgccttt tgaaaacaca cgttgatatc ttggatgatt tcagttatga
     3241 gggtactgtc gttccattga aagcattggc agagaaatac aagttcttga tatttgagga
     3301 cagaaaattc gccgatatcg gtaacacagt caaattacaa tatacatcgg gcgtttaccg
     3361 tatcgcagaa tggtctgata tcaccaacgc ccacggggtt actggtgctg gtattgttgc
     3421 tggcttgaaa caaggtgcgc aagaggtcac caaagaacca aggggattat tgatgcttgc
     3481 tgaattgtct tccaagggtt ctctagcaca cggtgaatat actaagggta ccgttgatat
     3541 tgcaaagagt gataaagatt tcgttattgg gttcattgct cagaacgata tgggaggaag
     3601 agaagaaggg tttgattggc taatcatgac cccaggtgta ggtttagacg acaaaggcga
     3661 tgcattgggt cagcagtaca gaaccgtcga cgaagttgta agtggtggat cagatatcat
     3721 cattgttggc agaggacttt tcgccaaggg tagagatcct aaggttgaag gtgaaagata
     3781 cagaaatgct ggatgggaag cgtaccaaaa gagaatcagc gctccccatt aattatacag
     3841 gaaacttaat agaacaaatc acatatttaa tctaatagcc acctgcattg gcacggtgca
     3901 acactcactt caacttcatc ttacaaaaga tcacgtgatc tgttgtattg ggatctctag
     3961 acctaataac ttcgtatagc atacattata cgaagttata ttaagggttg ctcgacttat
     4021 attgaatttt caaaaattct tacttttttt ttggatggac gcaaagaagt ttaataatca
     4081 tattacatgg cattaccacc atatacatat ccatatctaa tcttacttat atgttgtgga
     4141 aatgtaaaga gccccattat cttagcctaa aaaaaccttc tctttggaac tttcagtaat
     4201 acgcttaact gctcattgct atattgaagt acggattaga agccgccgag cgggcgacag
     4261 ccctccgacg gaagactctc ctccgtgcgt cctcgtcttc accggtcgcg ttcctgaaac
     4321 gcagatgtgc ctcgcgccgc actgctccga acaataaaga ttctacaata ctagctttta
     4381 tggttatgaa gaggaaaaat tggcagtaac ctggccccac aaaccttcaa attaacgaat
     4441 caaattaaca accataggat gataatgcga ttagtttttt agccttattt ctggggtaat
     4501 taatcagcga agcgatgatt tttgatctat taacagatat ataaatggaa aagctgcata
     4561 accactttaa ctaatacttt caacattttc agtttgtatt acttcttatt caaatgtcat
     4621 aaaagtatca acaaaaaatt gttaatatac ctctatactt taacgtcaag gagaaaaaac
     4681 tatagctagc aaaatggctt caggtgtaag taaatgcgaa accaaatgca caaaggcttg
     4741 ccctagacca gctccagttc ctaagttatt gattaaaggt ggtatggaag ttttggattt
     4801 ggttacaggt ccagattctg ttactgaaat cgaagcattt ttgaacccaa gaatgggtca
     4861 accaccaaca ccagaatctt taactgaagg tggtcaatat tacggttggt caagaggtat
     4921 taatttggct acatctgata ctgaagattc accaggtaat aatacattac caacttggtc
     4981 tatggcaaaa ttgcaattac caatgttgaa cgaagatttg acttgtgata ctttgcaaat
     5041 gtgggaagca gtttcagtta aaacagaagt tgttggttct ggttctttgt tggatgttca
     5101 tggttttaat aagccaacag atactgttaa cacaaagggt atttctactc cagttgaagg
     5161 ttcacaatat catgtttttg ctgttggtgg tgaaccattg gatttgcaag gtttagttac
     5221 agatgcaaga actaagtaca aggaagaagg tgttgttact attaaaacaa tcactaagaa
     5281 agatatggtt aataaggatc aagttttgaa cccaatttca aaggctaaat tggataagga
     5341 tggcatgtac ccagttgaaa tttggcatcc agatccagct aaaaatgaaa acacaagata
     5401 cttcggtaat tacactggtg gtactacaac tccaccagtt ttgcaattca ctaacacttt
     5461 gacaactgtt ttgttagatg aaaatggtgt tggtccatta tgtaaaggtg aaggtttgta
     5521 cttatcttgc gttgatatta tgggttggag agttacaaga aactacgatg ttcatcattg
     5581 gagaggtttg ccaagatact tcaagattac tttaagaaaa agatgggtta aaaatccata
     5641 cccaatggct tctttgattt cttctttgtt taataatatg ttaccacaag ttcaaggtca
     5701 accaatggaa ggtgaaaaca cacaagttga agaagttaga gtttacgatg gtactgaacc
     5761 agttccaggt gaccctgaca tgacaagata tgtagataga ttcggtaaaa ctaaaactgt
     5821 attccctggt aattaagcgg ccgc
//
