LOCUS       Exported                7239 bp ds-DNA     circular SYN 01-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 1, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
COMMENT     This file is created by VectorDesigner
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    108..679
                     /locus_tag="CMV\Promoter"
                     /label=CMV\Promoter
                     /note="/vntifkey=21"
     misc_feature    680..777
                     /locus_tag="R\region\of\5'\LTR"
                     /label=R\region\of\5'\LTR
                     /note="/vntifkey=21"
     misc_feature    778..860
                     /locus_tag="U5\region\of\5'\LTR"
                     /label=U5\region\of\5'\LTR
                     /note="/vntifkey=21"
     misc_feature    861..1011
                     /locus_tag="psi\packaging\signal"
                     /label=psi\packaging\signal
                     /note="/vntifkey=21"
     misc_feature    1013..1015
                     /locus_tag="gag\start\codon"
                     /label=gag\start\codon
                     /note="/vntifkey=21"
     misc_feature    1076..1078
                     /locus_tag="gag\stop\codon"
                     /label=gag\stop\codon
                     /note="/vntifkey=21"
     misc_feature    1375..1615
                     /locus_tag="RRE"
                     /label=RRE
                     /note="/vntifkey=21"
     misc_feature    2101..2130
                     /locus_tag="Splice\Acceptor"
                     /label=Splice\Acceptor
                     /note="/vntifkey=21"
     misc_feature    2167..2277
                     /locus_tag="cPPT"
                     /label=cPPT
                     /note="/vntifkey=21"
     misc_feature    2192..2216
                     /locus_tag="Pccl-1-F"
                     /label=Pccl-1-F
     promoter        2394..2642
                     /locus_tag="Human U6 Promoter"
                     /label=Human U6 Promoter
                     /note="/dnas_title=Human U6 Promoter"
                     /note="/vntifkey=29"
     misc_feature    2643..2661
                     /locus_tag="UL29"
                     /label=UL29
     misc_feature    2662..2665
                     /locus_tag="sp.gRNA"
                     /label=sp.gRNA
                     /note="U6 terminator"
     misc_feature    2666..2697
                     /locus_tag="optimized"
                     /label=optimized
     misc_feature    2698..2753
                     /locus_tag="sp.gRNA(1)"
                     /label=sp.gRNA
                     /label=sp.gRNA(1)
                     /note="U6 terminator"
     misc_feature    2760..2778
                     /locus_tag="YJ60-R"
                     /label=YJ60-R
     misc_feature    2762..2781
                     /locus_tag="PGK\Promoter"
                     /label=PGK\Promoter
                     /note="/vntifkey=21"
     primer_bind     2784..2807
                     /locus_tag="YJ287F"
                     /label=YJ287F
     misc_feature    2804..3393
                     /locus_tag="WPRE"
                     /label=WPRE
                     /note="/vntifkey=21"
     misc_feature    3482..3513
                     /locus_tag="U3\region\of\3'\LTR\(SIN)"
                     /label=U3\region\of\3'\LTR\(SIN)
                     /note="/vntifkey=21"
     primer_bind     complement(3494..3513)
                     /locus_tag="YJ635R"
                     /label=YJ635R
     promoter        3514..3762
                     /locus_tag="Human U6 Promoter(1)"
                     /label=Human U6 Promoter
                     /label=Human U6 Promoter(1)
                     /note="/dnas_title=Human U6 Promoter"
                     /note="/vntifkey=29"
     misc_feature    3763..3782
                     /locus_tag="UL8 gRNA"
                     /label=UL8 gRNA
     misc_feature    3783..3786
                     /locus_tag="sp.gRNA(2)"
                     /label=sp.gRNA
                     /label=sp.gRNA(2)
                     /note="U6 terminator"
     misc_feature    3787..3818
                     /locus_tag="optimized(1)"
                     /label=optimized
                     /label=optimized(1)
     misc_feature    3819..3874
                     /locus_tag="sp.gRNA(3)"
                     /label=sp.gRNA
                     /label=sp.gRNA(3)
                     /note="U6 terminator"
     misc_feature    3875..3897
                     /locus_tag="U3\region\of\3'\LTR\(SIN)(1)"
                     /label=U3\region\of\3'\LTR\(SIN)
                     /label=U3\region\of\3'\LTR\(SIN)(1)
                     /note="/vntifkey=21"
     misc_feature    3898..3993
                     /locus_tag="R\region\of\3'\LTR"
                     /label=R\region\of\3'\LTR
                     /note="/vntifkey=21"
     misc_feature    3994..4076
                     /locus_tag="U5\region\of\3'\LTR"
                     /label=U5\region\of\3'\LTR
                     /note="/vntifkey=21"
ORIGIN
        1 aagcttggcc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat
       61 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
      121 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgg ggtctctctg gttagaccag atctgagcct gggagctctc
      721 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
      781 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
      841 agtgtggaaa atctctagca gtggcgcccg aacagggacc tgaaagcgaa agggaaacca
      901 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
      961 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     1021 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     1081 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     1141 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     1201 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     1261 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     1321 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     1381 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     1441 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     1501 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     1561 cactatgggc gcagcctcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     1621 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     1681 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     1741 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     1801 gccttggaat gctagttgga gtaataaatc tctggaacag attggaatca cacgacctgg
     1861 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     1921 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     1981 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     2041 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     2101 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     2161 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     2221 gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac
     2281 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2341 aaacaaatta caaaaattca aaattttatc gatcacgaga ctagcctcga cgagagggcc
     2401 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg
     2461 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
     2521 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
     2581 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
     2641 ccgcgagcgt acacgtatcc cgtttcagag ctatgctgga aacagcatag caagttgaaa
     2701 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttggtcga
     2761 gtaccgggta ggggaggcgc tagcggcctc gagggaattc cgataatcaa cctctggatt
     2821 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     2881 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     2941 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     3001 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     3061 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     3121 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     3181 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     3241 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     3301 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     3361 cgagtcggat ctccctttgg gccgcctccc cgcatcggga attcgagctc ggtaccttta
     3421 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     3481 ctggaagggc taattcactc ccaacgaaga caagagggcc tatttcccat gattccttca
     3541 tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac
     3601 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
     3661 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
     3721 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccggggcagc cataccgcgt
     3781 aagtttcaga gctatgctgg aaacagcata gcaagttgaa ataaggctag tccgttatca
     3841 acttgaaaaa gtggcaccga gtcggtgctt ttttgatctg ctttttgctt gtactgggtc
     3901 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     3961 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4021 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     4081 ctagctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca
     4141 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt
     4201 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg
     4261 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag
     4321 cgaaccaatc agcagggtca tcgctagcct aggcttttgc gtcgagacgt acccaattcg
     4381 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     4441 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     4501 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4561 gaatggcgcg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4621 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4681 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4741 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4801 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4861 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4921 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4981 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttccca ggtggcactt
     5041 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5101 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     5161 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     5221 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     5281 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     5341 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     5401 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     5461 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5521 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5581 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5641 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5701 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5761 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5821 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5881 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5941 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6001 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6061 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6121 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6181 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6241 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6301 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     6361 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6421 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6481 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6541 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6601 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6661 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6721 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6781 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6841 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6901 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6961 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     7021 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     7081 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     7141 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     7201 caattaaccc tcactaaagg gaacaaaagc tggagctgc
//
