LOCUS       Exported                8472 bp ds-DNA     circular SYN 01-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 1, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
COMMENT     This file is created by VectorDesigner
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    108..679
                     /locus_tag="CMV\Promoter"
                     /label=CMV\Promoter
                     /note="/vntifkey=21"
     misc_feature    680..777
                     /locus_tag="R\region\of\5'\LTR"
                     /label=R\region\of\5'\LTR
                     /note="/vntifkey=21"
     misc_feature    778..860
                     /locus_tag="U5\region\of\5'\LTR"
                     /label=U5\region\of\5'\LTR
                     /note="/vntifkey=21"
     misc_feature    861..1011
                     /locus_tag="psi\packaging\signal"
                     /label=psi\packaging\signal
                     /note="/vntifkey=21"
     misc_feature    1013..1015
                     /locus_tag="gag\start\codon"
                     /label=gag\start\codon
                     /note="/vntifkey=21"
     misc_feature    1076..1078
                     /locus_tag="gag\stop\codon"
                     /label=gag\stop\codon
                     /note="/vntifkey=21"
     misc_feature    1375..1615
                     /locus_tag="RRE"
                     /label=RRE
                     /note="/vntifkey=21"
     misc_feature    2101..2130
                     /locus_tag="Splice\Acceptor"
                     /label=Splice\Acceptor
                     /note="/vntifkey=21"
     misc_feature    2167..2277
                     /locus_tag="cPPT"
                     /label=cPPT
                     /note="/vntifkey=21"
     misc_feature    2192..2216
                     /locus_tag="Pccl-1-F"
                     /label=Pccl-1-F
     promoter        2394..2642
                     /locus_tag="Human U6 Promoter"
                     /label=Human U6 Promoter
                     /note="/dnas_title=Human U6 Promoter"
                     /note="/vntifkey=29"
     misc_feature    2643..2661
                     /locus_tag="UL29 gRNA"
                     /label=UL29 gRNA
     misc_feature    2662..2665
                     /locus_tag="sp.gRNA"
                     /label=sp.gRNA
                     /note="U6 terminator"
     misc_feature    2666..2697
                     /locus_tag="optimized"
                     /label=optimized
     misc_feature    2698..2753
                     /locus_tag="sp.gRNA(1)"
                     /label=sp.gRNA
                     /label=sp.gRNA(1)
                     /note="U6 terminator"
     misc_feature    2762..3250
                     /locus_tag="PGK\Promoter"
                     /label=PGK\Promoter
                     /note="/vntifkey=21"
     CDS             3295..3314
                     /locus_tag="eGFP(1)"
                     /label=eGFP
                     /label=eGFP(1)
                     /note="/vntifkey=4"
     primer_bind     3295..3314
                     /locus_tag="YJ167F"
                     /label=YJ167F
     CDS             join(3315..3331,3352..4014)
                     /locus_tag="eGFP"
                     /label=eGFP
                     /note="/vntifkey=4"
     primer_bind     3332..3351
                     /locus_tag="YJ003F"
                     /label=YJ003F
     primer_bind     4017..4040
                     /locus_tag="YJ287F"
                     /label=YJ287F
     misc_feature    4037..4626
                     /locus_tag="WPRE"
                     /label=WPRE
                     /note="/vntifkey=21"
     misc_feature    4715..4746
                     /locus_tag="U3\region\of\3'\LTR\(SIN)"
                     /label=U3\region\of\3'\LTR\(SIN)
                     /note="/vntifkey=21"
     primer_bind     complement(4727..4746)
                     /locus_tag="YJ635R"
                     /label=YJ635R
     promoter        4747..4995
                     /locus_tag="Human U6 Promoter(1)"
                     /label=Human U6 Promoter
                     /label=Human U6 Promoter(1)
                     /note="/dnas_title=Human U6 Promoter"
                     /note="/vntifkey=29"
     misc_feature    4996..5015
                     /locus_tag="UL8 gRNA"
                     /label=UL8 gRNA
     misc_feature    5016..5019
                     /locus_tag="sp.gRNA(2)"
                     /label=sp.gRNA
                     /label=sp.gRNA(2)
                     /note="U6 terminator"
     misc_feature    5020..5051
                     /locus_tag="optimized(1)"
                     /label=optimized
                     /label=optimized(1)
     misc_feature    5052..5107
                     /locus_tag="sp.gRNA(3)"
                     /label=sp.gRNA
                     /label=sp.gRNA(3)
                     /note="U6 terminator"
     misc_feature    5108..5130
                     /locus_tag="U3\region\of\3'\LTR\(SIN)(1)"
                     /label=U3\region\of\3'\LTR\(SIN)
                     /label=U3\region\of\3'\LTR\(SIN)(1)
                     /note="/vntifkey=21"
     misc_feature    5131..5226
                     /locus_tag="R\region\of\3'\LTR"
                     /label=R\region\of\3'\LTR
                     /note="/vntifkey=21"
     misc_feature    5227..5309
                     /locus_tag="U5\region\of\3'\LTR"
                     /label=U5\region\of\3'\LTR
                     /note="/vntifkey=21"
ORIGIN
        1 aagcttggcc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat
       61 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
      121 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgg ggtctctctg gttagaccag atctgagcct gggagctctc
      721 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
      781 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
      841 agtgtggaaa atctctagca gtggcgcccg aacagggacc tgaaagcgaa agggaaacca
      901 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
      961 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     1021 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     1081 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     1141 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     1201 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     1261 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     1321 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     1381 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     1441 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     1501 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     1561 cactatgggc gcagcctcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     1621 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     1681 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     1741 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     1801 gccttggaat gctagttgga gtaataaatc tctggaacag attggaatca cacgacctgg
     1861 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     1921 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     1981 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     2041 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     2101 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     2161 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     2221 gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac
     2281 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2341 aaacaaatta caaaaattca aaattttatc gatcacgaga ctagcctcga cgagagggcc
     2401 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg
     2461 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
     2521 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
     2581 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
     2641 ccgcgagcgt acacgtatcc cgtttcagag ctatgctgga aacagcatag caagttgaaa
     2701 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttggtcga
     2761 gtaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     2821 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     2881 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
     2941 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
     3001 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
     3061 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
     3121 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
     3181 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
     3241 ctcttcctca tctccgggcc tttcgacctc tagcgggatc caccggtcgc caccatggtg
     3301 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     3361 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     3421 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3481 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     3541 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     3601 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3661 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3721 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     3781 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     3841 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     3901 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3961 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc
     4021 ctcgagggaa ttccgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4081 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4141 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4201 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4261 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     4321 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     4381 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     4441 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     4501 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     4561 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     4621 ccccgcatcg ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
     4681 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     4741 agacaagagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
     4801 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
     4861 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
     4921 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     4981 aaaggacgaa acaccggggc agccataccg cgtaagtttc agagctatgc tggaaacagc
     5041 atagcaagtt gaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
     5101 cttttttgat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5161 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5221 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5281 cttttagtca gtgtggaaaa tctctagcag catctagcta gaattaattc cgtgtattct
     5341 atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg
     5401 ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct
     5461 atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc
     5521 tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag
     5581 cctaggcttt tgcgtcgaga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     5641 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5701 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5761 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc
     5821 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5881 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5941 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6001 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6061 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6121 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6181 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     6241 atattaacgt ttacaatttc ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6301 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6361 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6421 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6481 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6541 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6601 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     6661 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6721 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6781 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6841 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6901 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6961 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7021 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7081 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7141 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7201 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7261 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7321 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7381 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7441 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7501 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7561 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7621 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7681 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7741 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7801 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7861 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7921 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7981 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8041 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8101 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8161 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8221 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     8281 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     8341 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     8401 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     8461 agctggagct gc
//
