LOCUS Exported 11545 bp ds-DNA circular SYN 01-FEB-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11545) AUTHORS Trial User TITLE Direct Submission JOURNAL Exported Monday, Feb 1, 2021 from SnapGene 5.2.4 https://www.snapgene.com FEATURES Location/Qualifiers source 1..11545 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 190..206 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 227..418 /label=tRNA promoter misc_feature 285..292 /label=TATA /note="TATA" misc_feature 322..418 /label=tRNA Ser /note="tRNA Ser (sup3-e WT)" misc_feature 419..488 /label=HDV /note="HDV + 3nt linker" misc_feature 485..488 /label=overhang misc_feature 490..495 /label=BsaI site promoter 496..625 /label=BBa_J72163 GlpT Promoter RBS 626..661 /label=sfGFP Ribosome Binding Site CDS 662..1378 /label=sfGFP terminator 1379..1507 /label=BBa_B0015 Terminator misc_feature 1508..1513 /label=BsaI site misc_RNA 1515..1594 /label=sgRNA - part 2 /label=sgRNA misc_feature 1515..1518 /label=overhang misc_feature 1600..1619 /label=sup terminator primer_bind complement(1640..1656) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 1664..1680 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1688..1718) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1733..1754 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(2042..2630) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2801..3661) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3662..3766) /gene="bla" /label=AmpR promoter promoter 3795..4536 /label=adh1 promoter /note="promoter for the Schizosaccharomyces pombe adh1 gene encoding alcohol dehydrogenase" misc_feature 4483..4490 /label=TAAATATA in adh15 misc_feature 4556 /label=start CDS 4557..8696 /codon_start=1 /label=pombe_cas9_complete /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGDSRADPKKKRKV" misc_feature 8673..8693 /label=SV40_NLS misc_feature 8697 /label=end terminator 8715..8958 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" rep_origin 9571..10352 /label=ars1 /note="Schizosaccharomyces pombe autonomously replicating sequence ars1" gene 10426..11545 /label=natMX6 /note="yeast selectable marker conferring nourseothricin resistance" promoter 10426..10769 /label=TEF promoter /note="Ashbya gossypii TEF promoter" CDS 10770..11342 /codon_start=1 /gene="Streptomyces noursei nat1" /product="nourseothricin acetyltransferase" /label=NrsR /note="confers resistance to nourseothricin" /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT LCGLDTALYDGTASDGEQALYMSMPCP" terminator 11348..11545 /label=TEF terminator /note="Ashbya gossypii TEF terminator" ORIGIN 1 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat 61 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 121 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 181 cacgacgttg taaaacgacg gccagtgttg taaaacgacg gccagtacca tttgagcatt 241 ggaaacttcc tatcaaaaat tacatagaaa tttttgattt tggctatata aattacattt 301 catgataaat gtacagctaa agtcactatg tccgagtggt taaggagtta gacttcaatc 361 ctgtattcta gtcatctaat gggctttgcc cgcgcaggtt caaatcctgc tggtgacgga 421 tggccggcat ggtcccagcc tcctcgctgg cgccggctgg gcaacacctt cgggtggcga 481 atgggactag agaccgaaag tgaaacgtga tttcatgcgt cattttgaac attttgtaaa 541 tcttatttaa taatgtgtgc ggcaattcac atttaattta tgaatgtttt cttaacatcg 601 cggcaactca agaaacggca ggttcggatc ttagctacta gagaaagagg agaaatacta 661 gatgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct attctggtgg aactggatgg 721 tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt gaaggtgacg caactaatgg 781 taaactgacg ctgaagttca tctgtactac tggtaaactg ccggttcctt ggccgactct 841 ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt tatccggacc atatgaagca 901 gcatgacttc ttcaagtccg ccatgccgga aggctatgtg caggaacgca cgatttcctt 961 taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa tttgaaggcg ataccctggt 1021 aaaccgcatt gagctgaaag gcattgactt taaagaggac ggcaatatcc tgggccataa 1081 gctggaatac aattttaaca gccacaatgt ttacatcacc gccgataaac aaaaaaatgg 1141 cattaaagcg aattttaaaa ttcgccacaa cgtggaggat ggcagcgtgc agctggctga 1201 tcactaccag caaaacactc caatcggtga tggtcctgtt ctgctgccag acaatcacta 1261 tctgagcacg caaagcgttc tgtctaaaga tccgaacgag aaacgcgatc atatggttct 1321 gctggagttc gtaaccgcag cgggcatcac gcatggtatg gatgaactgt acaaatgacc 1381 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 1441 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 1501 gtttataggt ctcagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 1561 caacttgaaa aagtggcacc gagtcggtgc tttttcattt ttttttgttt tttatgtctg 1621 tcatagctgt ttcctgtgtg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 1681 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 1741 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 1801 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 1861 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 1921 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 1981 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 2041 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 2101 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 2161 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 2221 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 2281 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 2341 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 2401 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 2461 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 2521 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 2581 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 2641 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 2701 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 2761 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 2821 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 2881 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 2941 gagaaccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 3001 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 3061 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 3121 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 3181 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 3241 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 3301 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 3361 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 3421 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 3481 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 3541 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 3601 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 3661 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 3721 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 3781 aagtgccacc tgacgcccta caacaactaa gaaaatggct atcatgcgga agctggtgag 3841 aagaacagca tcgggacaag ggaaggaaga acaaagacaa agaaaacaaa agaaagcaat 3901 tgaaaacaaa acaaaacaat tttcattcct tctcttatca ttccttttct tttcttttct 3961 ctcattcaac gcactccatc gtatccgtat tcctcttatt ttttctcttt ctctatatcc 4021 atttctttct ctctaggtgt gtcctctctc tctcttcaat ttctctactc cgcattccaa 4081 cgcatccttc ccccaacctc ccatttcctc cttacggccc gatagcgatc gtctttccct 4141 cgctatcact cgctaccggc ccctcctctg caccgtaacc tcctacgtat ttaccatatc 4201 ataaagtttt ttccgacgct tatcgctgac cccctgtcgc cctcctattg gcttccggat 4261 tatcttcttg tccataaggt gatccatgct tcctgaagat tcccgaaatg tgtccacttt 4321 ggcggggaat cattccatcc acttctttct ctctcgcttt cctcattcgg cgctcccctt 4381 ccgcgtctca ttggtcttcc gctccgtttt tgctttgccg atgttacttg gggagaggtg 4441 cgataatcct ttcgcaaaaa ctcggtttga cgcctcccat ggtataaata gtgggtggtg 4501 gacaggtgcc ttcgcttttc tttaagcaag agaattgctg caggtcgact ctagagatgg 4561 ataagaaata ctctattggc cttgatatcg gaactaacag tgtaggttgg gcagtcataa 4621 cagatgaata caaagttcca tctaagaaat ttaaagtcct tggaaacact gaccgacata 4681 gtataaaaaa gaatttaatc ggcgcattgc ttttcgactc tggtgaaacg gcagaagcca 4741 cccgattgaa acgaacagca agacgtcgat atacgcgaag aaagaaccgt atttgctatc 4801 ttcaggagat tttttctaat gaaatggcta aggtcgacga ctcctttttc cacagattgg 4861 aggaaagttt ccttgtcgaa gaggacaaaa aacacgagcg tcaccctatc ttcggaaaca 4921 ttgtagacga agtggcatat catgaaaagt atcctacgat ataccatttg cgtaaaaagt 4981 tggtcgactc aacggacaag gcagatttgc gtttgatata cttggctctt gcccacatga 5041 ttaaatttcg tggccatttc ttaattgaag gtgaccttaa cccagataac agtgatgtgg 5101 acaagttgtt cattcaattg gttcagacct acaatcaatt atttgaggaa aacccaatca 5161 atgcatctgg cgtagatgca aaggctattc tttccgcacg cctttcaaaa tcacgccgat 5221 tagagaatct tatcgctcag ttacctggcg agaagaaaaa tggcttgttc ggtaatttga 5281 tcgctttgag tttgggcctt acaccaaatt ttaagtccaa cttcgatctt gcagaagatg 5341 ccaaattaca attaagtaag gatacgtacg acgatgattt ggacaattta cttgcacaaa 5401 tcggtgacca atatgccgat cttttcttgg cagctaaaaa tttatcagac gcaattttat 5461 tgtccgatat ccttcgtgtg aacaccgaaa ttaccaaggc cccacttagt gcttccatga 5521 taaaacgcta tgatgagcat catcaggact taacattact taaagccttg gtgcgccaac 5581 aattacccga aaagtacaaa gaaatattct tcgaccagtc caagaacggt tatgccggct 5641 acatcgatgg tggtgcatca caggaggagt tctacaaatt cattaaacca atcttagaaa 5701 aaatggatgg taccgaagag ttattggtaa agttaaatcg cgaagacttg cttcgcaaac 5761 aacgaacatt cgacaacggc tccatccctc atcaaattca ccttggcgaa ttacatgcca 5821 tcttacgcag acaagaagac ttttatccat tcttgaagga taaccgcgag aaaattgaga 5881 agatccttac ctttagaatt ccatactacg taggcccttt ggctagaggc aactccagat 5941 ttgcatggat gaccagaaaa tctgaggaaa ccatcacgcc ctggaacttc gaagaagtcg 6001 tagataaggg cgcaagtgca cagtccttta ttgaaagaat gaccaatttt gataagaact 6061 tacctaacga aaaggtctta ccaaagcata gtttacttta tgaatacttc acggtgtaca 6121 acgaattaac aaaagtcaaa tacgttactg agggaatgag aaagcctgca ttcttgtccg 6181 gagaacaaaa gaaggccata gttgaccttc ttttcaaaac gaatcgaaag gtcacggtaa 6241 aacaacttaa ggaggattac tttaagaaga tagaatgctt tgatagtgtt gagatatcag 6301 gtgttgaaga ccgattcaat gcatcattgg gaacgtacca cgaccttttg aagatcataa 6361 aagataaaga ttttttagac aatgaggaga acgaagacat tttagaagac atcgttttga 6421 cacttacgtt atttgaggac agagagatga tcgaggagcg tcttaaaacg tatgcacatt 6481 tattcgacga caaagtgatg aaacaactta agcgcagaag atataccggt tggggaagac 6541 tttctcgaaa gttgatcaac ggcatccgcg ataagcaaag tggaaaaacc attttggatt 6601 tcttaaagtc tgatggcttt gctaatcgca acttcatgca attgattcat gacgattcat 6661 taactttcaa ggaagacata cagaaggccc aagtttcagg tcagggagat agtttgcacg 6721 agcacatcgc taatttagct ggttcccctg ctataaaaaa gggtatactt caaaccgtaa 6781 aagtcgtaga tgagttggta aaagtcatgg gcagacataa accagaaaat atagtaattg 6841 agatggcccg agaaaatcaa acgacgcaaa agggccaaaa aaactctaga gaaagaatga 6901 agcgtatcga ggagggcata aaggagttgg gttctcagat attgaaggag caccccgttg 6961 agaacactca gcttcagaat gagaagcttt atttatatta tttacaaaac ggtcgtgata 7021 tgtatgtgga tcaggagttg gacataaaca gattgtcaga ttacgatgtg gatcatatcg 7081 tcccccaatc attcttaaag gacgattcaa ttgacaacaa agttcttacg cgtagtgaca 7141 agaacagagg caaatcagac aatgtaccta gtgaggaagt agtgaaaaag atgaagaact 7201 attggagaca acttcttaat gctaagttaa taacgcagcg aaagtttgat aacttaacta 7261 aagccgaacg tggcggactt tcagagttgg ataaagcagg ctttatcaaa cgtcaattgg 7321 tagagactcg tcagatcact aagcatgtgg cccaaatact tgattcacgc atgaatacta 7381 agtacgatga aaacgataaa ttgattcgtg aggttaaggt aatcacgttg aaatctaagc 7441 ttgtttccga ctttcgtaag gattttcaat tctacaaggt tcgagagatt aataattacc 7501 accatgcaca tgacgcatac ttgaacgcag tcgtaggtac agcattgatc aagaagtacc 7561 caaaattaga gtcagaattt gtctacggtg actacaaagt atatgatgtc cgaaagatga 7621 tagccaagtc tgagcaagag ataggaaagg ccacagctaa gtattttttt tactccaaca 7681 ttatgaattt ctttaagaca gagattactt tagctaacgg tgaaatacga aaaagacccc 7741 ttatcgaaac gaatggagag acaggtgaga ttgtttggga caaaggtcga gatttcgcca 7801 ctgtgagaaa agtcttgagt atgccacagg tcaatattgt taagaaaaca gaagtacaga 7861 ctggtggatt ttctaaagaa tccattttgc ccaagagaaa ttccgacaaa cttattgcac 7921 gcaagaagga ttgggatccc aagaaatacg gcggtttcga cagtcccact gtagcatact 7981 cagtgttggt cgtggctaag gtggaaaagg gtaagtctaa gaagcttaaa tctgtgaagg 8041 aattgcttgg tatcacgatc atggagcgct catcttttga gaaaaatcct atagactttc 8101 ttgaagctaa aggctataaa gaggtgaaaa aggaccttat aattaagtta ccaaagtact 8161 ctcttttcga acttgagaac ggacgcaaga gaatgcttgc cagtgctgga gaattacaga 8221 agggtaatga gttagcattg ccatcaaagt acgtaaactt cttatattta gcctcccact 8281 atgagaaatt aaagggttca cccgaggaca atgaacaaaa gcaattattc gtagaacaac 8341 ataaacacta tttggacgaa attatagagc aaatctctga attttccaaa agagtgattt 8401 tagctgacgc aaatcttgat aaggttttat ctgcctataa caagcatcgc gacaaaccca 8461 tcagagaaca agccgaaaat atcattcact tattcacact tacgaatctt ggagcccctg 8521 ccgctttcaa gtattttgat actaccatag accgtaaacg ctatacgtct actaaggaag 8581 tcttagacgc cactcttatt caccaaagta taaccggctt gtatgagaca agaattgatt 8641 tgtctcaatt gggcggcgac agcagggctg accccaagaa gaagaggaag gtgtgatttt 8701 ggacctcgag tcatgtaatt agttatgtca cgcttacatt cacgccctcc ccccacatcc 8761 gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt 8821 atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac 8881 agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc 8941 tcgaaggctt taatttgcga tccccgtcta agaaaccatt attatcatga cattaaccta 9001 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 9061 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 9121 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 9181 tgcggcatca gagcagattg tactgagagt gcaccataat cgatggccaa ccttccaatt 9241 cattaaatcg agtcctccat cagatgattt acaaggtttt gcatagaatc cctaagtaag 9301 agaagttgtt agtgatttct tcaagtttac taaaatttac atacaaatgg atgatctctt 9361 ctccattgtt tcctttcttc ttgcaaacgc gttttacaaa gagatgacat gctattttct 9421 gattattttt ttctatagtt ttctattttg tacgtccttg ggagtccgaa atgtaaaatc 9481 ggggtatggt tagtagcggc agcgttcatt atggtatata aaagataaaa agtaggggca 9541 agcgagacag aatgggatac aagggcatcg tctataatta tagctaaaaa ttgtatttta 9601 atttgtattt tttgtaattt tatttctcat tgttttactt aaaatgaatg cgaattagag 9661 aaaacttcaa cgaaatgtca aaataagctc agcaaaatat acaattttag ggaaagcgat 9721 cagcaactta ttctccgttg tagattcttt ttcttcataa attccaacaa tgtaactgcc 9781 aattgatttt tctctagatg ctaataaatt gaaactaatg aatataataa cacaggtttt 9841 gataaattgt aaactcttac tcaaaaaaag aatgttaaaa ctaatttaac ctttttataa 9901 aggctcatgg cgttaaagag gatagtaaaa attctacaat tttttaattt aatgtcacaa 9961 ttgtcttgcg aatataattc tattaatttt cgctaactgc tgttttagta ctattacttt 10021 caatatgcct tatatgcaac tttaattcat gaagaatgca atatttttgc aatctttctt 10081 gtactgagcg ctttttatca tttagtactc attatttaat ttttcagtaa agggcaataa 10141 ataaatttgt agaagatgca atgtaatctc ctctatcctt ttgctcatat gtttatgagt 10201 atacctagtc tagaaaggct tgtatttaaa atattattca ataaaaattc acaattttta 10261 cgacatgtgc taccattcac ttaacttcct gattataaaa ttggttcgtt tatactaatt 10321 acttaagtac ctttaactaa acaaaatgcc tatatatata ttaatttaca atgagtgtca 10381 gataagtcac tatgtccgag tggttaagga gttagactcg ccatcgacat ggaggcccag 10441 aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg 10501 tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt tgatggccgc 10561 acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg aaacgctccc 10621 ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat aaaaggttag 10681 gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct aggatacagt 10741 tctcacatca catccgaaca taaacaacca tgggtaccac tcttgacgac acggcttacc 10801 ggtaccgcac cagtgtcccg ggggacgccg aggccatcga ggcactggat gggtccttca 10861 ccaccgacac cgtcttccgc gtcaccgcca ccggggacgg cttcaccctg cgggaggtgc 10921 cggtggaccc gcccctgacc aaggtgttcc ccgacgacga atcggacgac gaatcggacg 10981 acggggagga cggcgacccg gactcccgga cgttcgtcgc gtacggggac gacggcgacc 11041 tggcgggctt cgtggtcgtc tcgtactccg gctggaaccg ccggctgacc gtcgaggaca 11101 tcgaggtcgc cccggagcac cgggggcacg gggtcgggcg cgcgttgatg gggctcgcga 11161 cggagttcgc ccgcgagcgg ggcgccgggc acctctggct ggaggtcacc aacgtcaacg 11221 caccggcgat ccacgcgtac cggcggatgg ggttcaccct ctgcggcctg gacaccgccc 11281 tgtacgacgg caccgcctcg gacggcgagc aggcgctcta catgagcatg ccctgcccct 11341 aatcagtact gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt 11401 ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct 11461 cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg 11521 tatgtgaatg ctggtcgcta tactg //