LOCUS       pcDNA3.1+(Zeo)-FLAG-muGFP.xdna    5702 bp      DNA     circular   31/01/2021
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : pcDNA3.1-FLAG-sacB.xdna [4981  nt] : (NheI[288
            4] / BamHI[943]) to PCR(pIQ-usGFP F233D.xdna) [713  nt] : (B
            amHI[7] / SpeI[724]) - MONOMERIC usGFP
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             complement(4706..5566)
                     /label=Amp(R)
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=invitrogen="1020000" - vntifkey="4" - label=Amp(R) - complement(4019..4879) \ 
     polyA_site      1715..1939
                     /label=BGH\pA
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=invitrogen="1870000" - vntifkey="26" - label=BGHpA - 1028..1252 \ 
     primer          complement(1709..1726)
                     /label=BGH\reverse\primer
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=invitrogen="1910000" - vntifkey="27" - label=BGHreverseprimer - complement(1022..1039) \ 
     promoter        complement(5567..5665)
                     /label=bla\promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=invitrogen="2330000" - vntifkey="30" - label=blapromoter - complement(4880..4978) \ 
     primer          769..789
                     /label=CMV\forward\primer
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=invitrogen="1930000" - vntifkey="27" - label=CMVforwardprimer - 769..789 \ 
     promoter        232..819
                     /label=CMV\promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=invitrogen="2180000" - vntifkey="29" - label=CMVpromoter - 232..819 \ 
     promoter        2803..2870
                     /label=EM7\promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=invitrogen="2340000" - vntifkey="30" - label=EM7promoter - 2116..2183 \ 
     rep_origin      1985..2413
                     /label=f1\origin
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=invitrogen="2500000" - vntifkey="33" - label=f1origin - 1298..1726 \ 
     rep_origin      complement(3888..4561)
                     /label=pUC\origin
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=invitrogen="2540000" - vntifkey="33" - label=pUCorigin - complement(3201..3874) \ 
     promoter        2418..2788
                     /label=SV40\early\promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=invitrogen="2290000" - vntifkey="29" - label=SV40earlypromoter - 1731..2101 \ 
     polyA_signal    3375..3505
                     /label=SV40\pA
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=invitrogen="1840000" - vntifkey="25" - label=SV40pA - 2688..2818 \ 
     primer          863..882
                     /label=T7\primer
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=invitrogen="2100000" - vntifkey="27" - label=T7primer - 863..882 \ 
     promoter        863..879
                     /label=T7\promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=invitrogen="2370000" - vntifkey="30" - label=T7promoter - 863..879 \ 
     CDS             2871..3245
                     /label=Zeo(R)
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=invitrogen="1370000" - vntifkey="4" - label=Zeo(R) - 2184..2558 \ 
     misc_feature    916..936
                     /label=FLAG
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             949..1659
                     /label=mustGFP
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="4" - label=SFGFP - 151..861 \ 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagT 
      901 GCCACCCCAT GGATGGATTA CAAAGATGAT GATGATGTCG ACGgatcCAG CAAAGGAGAA 
      961 GAACTTTTCA CTGGAGTTGT Cccaattctt gttgaattag atggtgatgt taatgggcac 
     1021 aaattttctg tccgtggaga gggtgaaggt gatgctacaa acggaaaact cacccttaaa 
     1081 tttatttgca ctactggaaa actacctgtt ccgtggccaa cacttgtcac tactctgacc 
     1141 tatggtgttc tatgcttttc ccgttatccg gatcacatga aacggcatga ctttttcaag 
     1201 agtgccatgc ccgaaggtta tgtacaggaa cgcactatat ctttcaaaga tgacgggacc 
     1261 tacaagacgc gtgctgaagt caagtttgaa ggtgataccc ttgttaatcg tatcgagtta 
     1321 aaaggtattg attttaaaga agatggaaac attctcggac acaaactgga gtacaacttt 
     1381 aactcacaca atgtatacat cacggcagac aaacaaaaga atggaatcaa agcttacttc 
     1441 aaaattcgcc acaacgttga agatggttcc gttcaactag cagaccatta tcaacaaaat 
     1501 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctctc gacacaatct 
     1561 gttctttcga aagatcccaa cgaaaagcgt gaccacatgg tccttcttga ggatgtaact 
     1621 gctGCTGGGA TTACACATGG CATGGATGAA CTCTACAAAa ctagCTGATA ACtcgagtct 
     1681 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct 
     1741 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt 
     1801 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg 
     1861 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg 
     1921 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat 
     1981 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 
     2041 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 
     2101 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga 
     2161 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 
     2221 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 
     2281 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 
     2341 ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 
     2401 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 
     2461 ccccaggcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 
     2521 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 
     2581 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 
     2641 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc 
     2701 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag 
     2761 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat 
     2821 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt 
     2881 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga 
     2941 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg 
     3001 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg 
     3061 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca 
     3121 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc 
     3181 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg 
     3241 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg 
     3301 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt 
     3361 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 
     3421 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 
     3481 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat 
     3541 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 
     3601 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 
     3661 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 
     3721 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 
     3781 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 
     3841 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 
     3901 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 
     3961 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 
     4021 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 
     4081 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 
     4141 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 
     4201 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 
     4261 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 
     4321 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 
     4381 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 
     4441 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 
     4501 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 
     4561 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 
     4621 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 
     4681 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 
     4741 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 
     4801 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 
     4861 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 
     4921 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 
     4981 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 
     5041 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 
     5101 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 
     5161 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 
     5221 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 
     5281 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 
     5341 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 
     5401 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 
     5461 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 
     5521 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 
     5581 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
     5641 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
     5701 tc
//