LOCUS       pAM-DCA-musGFP.xdna    6116 bp      DNA     circular   20/03/2015
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : pAM-DCA-EcoRI-EGFP.xdna [5373  nt] : (HindIII[
            1928] / BamHI[1180]) to PCR(pIQ-usGFP F233D HindIIIFix.xdna)
             [735  nt] : (BamHI[7] / HindIII[746])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    1153..1158
                     /label=BamHI-BglII-cohesive\ends
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="14" - label=BamHI-BglII-cohesiveends - 1153..1158 \ 
     primer_bind     279..299
                     /label=Khai\See\REV
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="28" - label=KhaiSeeREV - 279..299 \ 
     primer_bind     1093..1112
                     /label=Ollie\FWD
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="28" - label=OllieFWD - 1093..1112 \ 
     CDS             complement(4341..5191)
                     /label=Amp\R
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="4" - label=AmpR - complement(4350..5200) \ 
     enhancer        complement(3022..3204)
                     /label=SV40
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="9" - label=SV40 - complement(3031..3213) \ 
     promoter        466..1050
                     /label=Chicken\Beta\Actin\Promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="29" - label=ChickenBetaActinPromoter - 466..1050 \ 
     enhancer        216..439
                     /label=CMV\IE
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="9" - label=CMVIE - 216..439 \ 
     misc_feature    2000..2523
                     /label=WPRE
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="21" - label=WPRE - 2009..2532 \ 
     primer_bind     1099..1115
                     /label=#725
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="28" - label=#725 - 1099..1115 \ 
     primer_bind     2036..2055
                     /label=#721
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="28" - label=#721 - 2045..2064 \ 
     misc_feature    1202..1912
                     /label=musGFP
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 ttctagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg 
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc 
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca 
      181 tgctctaggt acgacgtact agtagtagga tggtaccatt gacgtcaata atgacgtatg 
      241 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggac tatttacggt 
      301 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
      361 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
      421 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca 
      481 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta 
      541 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg 
      601 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc 
      661 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata 
      721 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgacgct gccttcgccc cgtgccccgc 
      781 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg 
      841 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt 
      901 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg 
      961 gggagcggct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc 
     1021 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc 
     1081 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt 
     1141 tggcaaagaa ttggatcttg gaattcaagc tgctagcaag gatccaccgg tcgccaccat 
     1201 gAGCAAAGGA GAAGAACTTT TCACTGGAGT TGTCccaatt cttgttgaat tagatggtga 
     1261 tgttaatggg cacaaatttt ctgtccgtgg agagggtgaa ggtgatgcta caaacggaaa 
     1321 actcaccctt aaatttattt gcactactgg aaaactacct gttccgtggc caacacttgt 
     1381 cactactctg acctatggtg ttctatgctt ttcccgttat ccggatcaca tgaaacggca 
     1441 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaacgcacta tatctttcaa 
     1501 agatgacggg acctacaaga cgcgtgctga agtcaagttt gaaggtgata cccttgttaa 
     1561 tcgtatcgag ttaaaaggta ttgattttaa agaagatgga aacattctcg gacacaaact 
     1621 ggagtacaac tttaactcac acaatgtata catcacggca gacaaacaaa agaatggaat 
     1681 caaagcgtac ttcaaaattc gccacaacgt tgaagatggt tccgttcaac tagcagacca 
     1741 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct 
     1801 ctcgacacaa tctgttcttt cgaaagatcc caacgaaaag cgtgaccaca tggtccttct 
     1861 tgaggatgta actgctGCTG GGATTACACA TGGCATGGAT GAACTCTACA AATAATAGAa 
     1921 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa 
     1981 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat 
     2041 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta 
     2101 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc 
     2161 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt 
     2221 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg 
     2281 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc 
     2341 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc 
     2401 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct 
     2461 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca 
     2521 tcgataccgt cgactcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt 
     2581 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc 
     2641 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt 
     2701 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat 
     2761 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgac tagagcatgg 
     2821 ctacgtagat aagtagcatg gcgggttaat cattaactac aaggaacccc tagtgatgga 
     2881 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc 
     2941 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gagctttttg 
     3001 caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag 
     3061 gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg 
     3121 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac 
     3181 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac 
     3241 ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg 
     3301 gactttccac accctaactg acacacattc cacagctgca ttaatgaatc ggccaacgcg 
     3361 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 
     3421 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 
     3481 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 
     3541 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
     3601 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
     3661 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
     3721 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 
     3781 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
     3841 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
     3901 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
     3961 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 
     4021 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
     4081 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
     4141 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
     4201 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 
     4261 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 
     4321 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 
     4381 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 
     4441 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 
     4501 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 
     4561 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 
     4621 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 
     4681 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 
     4741 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 
     4801 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 
     4861 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 
     4921 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 
     4981 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 
     5041 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 
     5101 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 
     5161 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 
     5221 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 
     5281 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta 
     5341 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt 
     5401 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 
     5461 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 
     5521 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccattcga 
     5581 cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg 
     5641 agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc 
     5701 acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc 
     5761 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg 
     5821 gtgatgccgg ccacgatgcg tccggcgtag aggatctggc tagcgatgac cctgctgatt 
     5881 ggttcgctga ccatttccgg gtgcgggacg gcgttaccag aaactcagaa ggttcgtcca 
     5941 accaaaccga ctctgacggc agtttacgag agagatgata gggtctgctt cagtaagcca 
     6001 gatgctacac aattaggctt gtacatattg tcgttagaac gcggctacaa ttaatacata 
     6061 accttatgta tcatacacat acgatttagg tgacactata gaatacacgg aattaa
//