LOCUS       pCSN075                11049 bp    DNA     circular UNA 02-FEB-2021
DEFINITION  Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A
            fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae.
ACCESSION   urn.local...17-cyu21nw
VERSION     urn.local...17-cyu21nw
KEYWORDS    .
SOURCE      synthetic DNA construct (pCSN075)
  ORGANISM  synthetic DNA construct
            .
FEATURES             Location/Qualifiers
     promoter        1..600
                     /created_by="User"
                     /modified_by="User"
                     /label="Sc_TEF1.pro"
     gene            601..4443
                     /gene="dLbCas12a_NLS_Mxi1"
                     /created_by="User"
                     /label="dLbCas12a_NLS_Mxi1"
     CDS             601..4443
                     /codon_start=1
                     /gene="dLbCas12a_E925A_NLS_Mxi1"
                     /product="DNase-dead CRISPR-associated endonuclease Cas12a
                     fused to SV40 NLS and Mxi1 repression domain"
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRA
                     EDYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLR
                     KEIAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNREN
                     MFSEEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVED
                     FFEGEFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPL
                     YKQVLSDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSA
                     GIFVKNGPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKI
                     GSFSLEQLQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKND
                     AVVAIMKDLLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIR
                     NYVTQKPYSKDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCL
                     QKIDKDDVNGNYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKG
                     DMFNLNDCHKLIDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSF
                     ESASKKEVDKLVEEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSG
                     GAELFMRRASLKKEELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHI
                     PIAINKCPKNIFKINTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSL
                     NEIINNFNGIRIKTDYHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICEL
                     VEKYDAVIALADLNSGFKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALK
                     GYQITNKFESFKSMSTQNGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISS
                     FDRIMYVPEEDLFEFALDYKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEE
                     VCLTSAYKELFNKYGINYQQGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTD
                     VDFLISPVKNSDGIFYDSRNYEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDE
                     KLDKVKIAISNKEWLEYAQTSVKHSRADPKKKRKVMERVRMINVQRLLEAAEFLERRE
                     RECEHGYASSFPSMPSPR"
                     /modified_by="User"
                     /label="dLbCas12a_NLS_Mxi1"
     terminator      4444..4744
                     /created_by="User"
                     /label="GND2.ter"
     protein_bind    complement(4789..4822)
                     /bound_moiety="Cre recombinase"
                     /note="loxP mutant. Cre-mediated recombination occurs in 
                     the 8-bp core sequence (GCATACAT)."
                     /label="lox66"
     promoter        4868..5211
                     /note="Ashbya gossypii TEF promoter"
                     /label="TEF promoter"
     gene            5212..6021
                     /gene="KanR"
                     /created_by="User"
                     /label="KanR"
     CDS             5212..6021
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
                     GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /modified_by="User"
                     /label="KanR"
     terminator      6027..6224
                     /note="Ashbya gossypii TEF terminator"
                     /label="TEF terminator"
     protein_bind    complement(6272..6305)
                     /bound_moiety="Cre recombinase"
                     /note="loxP mutant. Cre-mediated recombination occurs in 
                     the 8-bp core sequence (GCATACAT)."
                     /label="lox71"
     protein_bind    6436..6452
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     protein_bind    6505..6526
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6814..7402)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     gene            complement(7573..8433)
                     /gene="AmpR"
                     /created_by="User"
                     /label="AmpR"
     CDS             complement(7573..8433)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /modified_by="User"
                     /label="AmpR"
     promoter        complement(8434..8538)
                     /gene="bla"
                     /label="AmpR promoter"
     misc_feature    8575..9078
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
                     /label="CEN/ARS"
     promoter        9337..9617
                     /gene="S. cerevisiae TRP1"
                     /label="TRP1 promoter"
     CDS             9618..10292
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK"
                     /modified_by="User"
                     /label="TRP1"
     gene            9618..10292
                     /gene="TRP1"
                     /created_by="User"
                     /label="TRP1"
     rep_origin      complement(10395..10850)
                     /direction="LEFT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN      
        1 ttggctgata atagcgtata aacaatgcat actttgtacg ttcaaaatac aatgcagtag
       61 atatatttat gcatattaca tataatacat atcacatagg aagcaacagg cgcgttggac
      121 ttttaatttt cgaggaccgc gaatccttac atcacaccca atcccccaca agtgatcccc
      181 cacacaccat agcttcaaaa tgtttctact ccttttttac tcttccagat tttctcggac
      241 tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat ttcccctctt
      301 tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa aaaagacacc
      361 gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat ttttatcacg tttctttttc
      421 ttgaaaattt ttttttttga tttttttctc tttcgatgac ctcccattga tatttaagtt
      481 aataaacggt cttcaatttc tcaagtttca gtttcatttt tcttgttcta ttacaacttt
      541 ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt taattacaaa
      601 atgtctaagt tggaaaaatt caccaactgt tactctttgt ctaagacttt gagattcaag
      661 gccatcccag ttggtaagac ccaagaaaac atcgacaaca agagactatt agttgaagat
      721 gaaaagagag ctgaagacta caagggtgtc aagaaattgt tggacagata ctacttgtct
      781 tttatcaacg acgttttgca ttccatcaag ctaaagaact tgaataacta catctctttg
      841 ttcagaaaga agactagaac tgaaaaggaa aataaggaat tggaaaactt ggaaatcaac
      901 ttgagaaagg aaattgctaa ggctttcaag ggtaatgaag gttacaagtc tttattcaag
      961 aaagacatca ttgaaaccat tttgccagaa tttttggatg ataaggatga aattgctttg
     1021 gttaactctt tcaacggttt caccactgct ttcactggtt tcttcgacaa cagagaaaac
     1081 atgttctccg aggaagctaa atccacttct attgctttca gatgtatcaa cgaaaacttg
     1141 acccgttaca tctctaacat ggacattttt gaaaaggtcg acgccatctt tgacaagcac
     1201 gaagtccaag aaatcaagga aaagatctta aactccgact acgatgtcga agatttcttc
     1261 gaaggtgaat tcttcaactt tgttttaacc caagaaggta tcgatgtcta caacgccatt
     1321 atcggtggtt ttgtcactga atctggtgaa aagatcaagg gtttgaacga atacattaac
     1381 ttgtacaacc aaaagaccaa acaaaaattg ccaaagttca agccattgta caagcaagtt
     1441 ttgtctgaca gagaatcttt gtctttttac ggtgaagggt acacctctga cgaagaagtc
     1501 ttggaagtct tcagaaacac tttgaacaag aactctgaaa tcttctcctc catcaagaag
     1561 ttagaaaagt tgttcaagaa cttcgatgaa tactcttctg ctggtatctt cgttaagaac
     1621 ggtccagcca tctctaccat ttctaaggat atctttggtg aatggaacgt cattagagac
     1681 aaatggaacg ctgaatacga tgacatccat ttgaagaaaa aggctgttgt caccgaaaag
     1741 tacgaagacg acagaagaaa atccttcaag aagatcggtt ccttctcctt ggaacaatta
     1801 caagaatacg ccgatgccga tttgtccgtt gtcgaaaaat tgaaggaaat tattattcaa
     1861 aaggttgatg aaatttacaa agtttacggt tcctctgaaa agttattcga tgctgatttc
     1921 gtcttggaaa agtctttgaa gaagaacgac gctgttgtcg ctatcatgaa ggacttgttg
     1981 gactctgtca aatctttcga aaactatatc aaggccttct tcggtgaagg taaggaaact
     2041 aacagagatg aatccttcta cggtgacttt gtcttggctt acgatatttt gttgaaggtt
     2101 gaccacatct acgatgccat cagaaactac gttactcaaa agccatactc taaggacaaa
     2161 ttcaagttgt acttccaaaa cccacaattc atgggtggtt gggataagga caaggaaact
     2221 gactacagag ctaccatttt gagatacggt tccaagtact acttggccat catggacaag
     2281 aagtacgcca agtgtttgca aaagattgac aaggacgatg tcaacggtaa ctacgaaaag
     2341 attaactaca agttgttgcc aggtccaaac aagatgttgc caaaggtttt cttctccaaa
     2401 aagtggatgg cttactacaa cccatctgaa gacatccaaa agatctacaa gaacggtact
     2461 ttcaaaaagg gtgacatgtt caacttaaac gactgtcaca agttgatcga cttcttcaag
     2521 gactccatct ctagataccc aaaatggtcc aacgcttacg atttcaactt ctctgaaact
     2581 gaaaaataca aggatattgc tggtttctac cgtgaagtcg aggaacaagg ttataaggtt
     2641 tctttcgaat ccgcttctaa gaaagaagtt gacaaattag tcgaagaagg taagttgtac
     2701 atgttccaaa tctacaacaa agatttctcc gacaagtctc acggtactcc aaacttgcac
     2761 accatgtact tcaagttgct attcgatgaa aacaaccacg gtcaaatcag attgtctggt
     2821 ggtgctgaat tgttcatgag acgtgcttct ctaaagaagg aagaattagt cgtccaccca
     2881 gctaactctc caattgccaa caagaaccca gacaacccta agaagaccac cactttgtcc
     2941 tacgacgttt acaaggacaa gagattctcc gaagaccaat acgaattgca cattccaatt
     3001 gctatcaaca agtgtccaaa gaacatcttc aagatcaaca ctgaagtcag agttttgtta
     3061 aagcacgatg acaaccctta cgttattggt atcgaccgtg gtgaaagaaa tttgttgtac
     3121 attgttgttg ttgacggtaa gggtaacatc gttgaacaat actccttgaa cgaaatcatc
     3181 aacaacttca acggtattag aatcaagact gattaccact ctttgttgga taagaaggaa
     3241 aaggaacgtt ttgaagctcg tcaaaactgg acctctattg aaaacatcaa agaattgaag
     3301 gctggttaca tcagtcaagt tgtccacaag atctgtgaat tggtcgagaa gtacgatgcc
     3361 gttattgcct tggcagattt gaactctggt tttaagaact ctcgtgtcaa ggttgaaaag
     3421 caagtctacc aaaagttcga aaagatgtta atcgacaaat tgaactacat ggttgacaag
     3481 aaatccaacc catgtgctac cggtggtgct ttgaaaggtt accaaatcac caacaaattc
     3541 gaatctttca aatctatgtc cactcaaaac gggttcatct tctacattcc agcttggttg
     3601 acctccaaga tcgacccatc taccggtttc gttaacttgt tgaagaccaa gtacacttcc
     3661 attgctgatt ccaagaagtt catctcttct ttcgacagaa tcatgtacgt tccagaagaa
     3721 gacttgttcg aattcgcctt ggactataag aacttctcca gaaccgatgc tgactacatt
     3781 aagaaatgga aattgtactc ctacggtaac agaatcagaa ttttcagaaa cccaaagaaa
     3841 aacaacgttt tcgattggga agaagtttgt ttgacttctg cctacaagga attattcaac
     3901 aaatacggta tcaactacca acaaggtgat atcagagctt tgttgtgtga acaatctgac
     3961 aaggctttct actcttcctt catggctttg atgtccttga tgttgcaaat gagaaactcc
     4021 atcactggta gaactgatgt cgacttcctc atttctccag ttaagaattc tgacggtatt
     4081 ttctacgact ctagaaatta cgaagctcaa gaaaacgcta ttttgccaaa gaacgctgat
     4141 gctaacggtg cttacaatat tgctagaaag gttttgtggg ctatcggtca attcaagaag
     4201 gctgaagacg aaaagctaga caaggtcaag attgctattt ctaacaagga atggttggaa
     4261 tacgctcaaa cctccgtcaa gcactccaga gctgatccaa agaagaagag aaaggtaatg
     4321 gaacgtgtca gaatgatcaa cgttcaaaga ttgttggaag ctgctgaatt cttggaaaga
     4381 agagaaagag aatgtgaaca cggttacgcc tcctctttcc catccatgcc atctccaaga
     4441 taaaggagtt aaaggcaaag ttttcttttc tagagccgtt cccacaaata attatacgta
     4501 tatgcttctt ttcgtttact atatatctat atttacaagc ctttattcac tgatgcaatt
     4561 tgtttccaaa tacttttttg gagatctcat aactagatat catgatggcg caacttggcg
     4621 ctatcttaat tactctggct gccaggcccg tgtagagggc cgcaagacct tctgtacgcc
     4681 atatagtctc taagaacttg aacaagtttc tagacctatt gccgcctttc ggatcgctat
     4741 tgttgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acgaattcta ccgttcgtat
     4801 aatgtatgct atacgaagtt atagatctgt ttagcttgcc tcgtccccgc cgggtcaccc
     4861 ggccagcgac atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg
     4921 ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc
     4981 atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcagac
     5041 ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct
     5101 gtagagaaat ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt
     5161 taaaatcttg ctaggataca gttctcacat cacatccgaa cataaacaac catgggtaag
     5221 gaaaagactc acgtttcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     5281 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg
     5341 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     5401 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     5461 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggcaaaaca
     5521 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     5581 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     5641 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     5701 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataagctt
     5761 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     5821 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     5881 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     5941 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     6001 atgctcgatg agtttttcta atcagtactg acaataaaaa gattcttgtt ttcaagaact
     6061 tgtcatttgt atagtttttt tatattgtag ttgttctatt ttaatcaaat gttagcgtga
     6121 tttatatttt ttttcgcctc gacatcatct gcccagatgc gaagttaagt gcgcagaaag
     6181 taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg attcgatact
     6241 aacgccgcca tccagtgtcg aaaacgagct cataacttcg tataatgtat gctatacgaa
     6301 cggtagaatt cgatatcaga tccactagtg gcctatgcgg ccgccaccgc ggtggagctc
     6361 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     6421 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     6481 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     6541 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6601 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6661 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6721 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6781 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6841 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6901 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6961 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7021 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7081 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7141 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7201 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     7261 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7321 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7381 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7441 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7501 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7561 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7621 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7681 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7741 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7801 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7861 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7921 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7981 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8041 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8101 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8161 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8221 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8281 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8341 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8401 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8461 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8521 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcct tttcatcacg
     8581 tgctataaaa ataattataa tttaaatttt ttaatataaa tatataaatt aaaaatagaa
     8641 agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc cgaagatgta aaagactcta
     8701 gggggatcgc caacaaatac taccttttat cttgctcttc ctgctctcag gtattaatgc
     8761 cgaattgttt catcttgtct gtgtagaaga ccacacacga aaatcctgtg attttacatt
     8821 ttacttatcg ttaatcgaat gtatatctat ttaatctgct tttcttgtct aataaatata
     8881 tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt gtttattttt ttttcttcat
     8941 tccgtaactc ttctaccttc tttatttact ttctaaaatc caaatacaaa acataaaaat
     9001 aaataaacac agagtaaatt cccaaattat tccatcatta aaagatacga ggcgcgtgta
     9061 agttacaggc aagcgatccg tctaagaaac cattattatc atgacattaa cctataaaaa
     9121 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     9181 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     9241 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     9301 atcagagcag attgtactga gagtgcacca tagatcacga cattactata tatataatat
     9361 aggaagcatt taatagaaca gcatcgtaat atatgtgtac tttgcagtta tgacgccaga
     9421 tggcagtagt ggaagatatt ctttattgaa aaatagcttg tcaccttacg tacaatcttg
     9481 atccggagct tttctttttt tgccgattaa gaattaattc ggtcgaaaaa agaaaaggag
     9541 agggccaaga gggagggcat tggtgactat tgagcacgtg agtatacgtg attaagcaca
     9601 caaaggcagc ttggagtatg tctgttatta atttcacagg tagttctggt ccattggtga
     9661 aagtttgcgg cttgcagagc acagaggccg cagaatgtgc tctagattcc gatgctgact
     9721 tgctgggtat tatatgtgtg cccaatagaa agagaacaat tgacccggtt attgcaagga
     9781 aaatttcaag tcttgtaaaa gcatataaaa atagttcagg cactccgaaa tacttggttg
     9841 gcgtgtttcg taatcaacct aaggaggatg ttttggctct ggtcaatgat tacggcattg
     9901 atatcgtcca actgcatgga gatgagtcgt ggcaagaata ccaagagttc ctcggtttgc
     9961 cagttattaa aagactcgta tttccaaaag actgcaacat actactcagt gcagcttcac
    10021 agaaacctca ttcgtttatt cccttgtttg attcagaagc aggtgggaca ggtgaacttt
    10081 tggattggaa ctcgatttct gactgggttg gaaggcaaga gagccccgaa agcttacatt
    10141 ttatgttagc tggtggactg acgccagaaa atgttggtga tgcgcttaga ttaaatggcg
    10201 ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa
    10261 tagcaaattt cgtcaaaaat gctaagaaat aggttattac tgagtagtat ttatttaagt
    10321 attgtttgtg cacttgccga tctatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    10381 aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
    10441 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
    10501 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
    10561 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
    10621 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
    10681 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
    10741 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
    10801 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca
    10861 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
    10921 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
    10981 tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa
    11041 ttgggtacc
//
