LOCUS       Exported                5150 bp ds-DNA     circular SYN 02-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 2, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
COMMENT     SECID/File created by Clone Manager, Scientific
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    10..562
                     /gene="CMV_immearly_promoter"
                     /label=CMV_immearly_promoter
                     /note="/SECDrawAs=Region"
                     /note="/SECStyleId=1"
     misc_feature    65..352
                     /gene="CAG_enhancer"
                     /label=CAG_enhancer
                     /note="/SECDrawAs=Region"
                     /note="/SECStyleId=1"
     misc_feature    520..589
                     /gene="CMV promoter"
                     /label=CMV promoter
                     /note="/SECDrawAs=Region"
                     /note="/SECStyleId=1"
     CDS             613..1329
                     /codon_start=1
                     /gene="Venus"
                     /label=Venus
                     /note="/SECDrawAs=Gene"
                     /note="/SECStyleId=1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     ERVTAAGITLGMDELYK"
     CDS             1330..1338
                     /gene="3aa Linker"
                     /label=3aa Linker
                     /note="/SECDrawAs=Gene"
                     /note="/SECStyleId=1"
     CDS             1339..1761
                     /codon_start=1
                     /gene="BimL"
                     /label=BimL
                     /note="/SECDrawAs=Gene"
                     /note="/SECStyleId=1"
                     /translation="MAKQPSDVSSECDREGGQLQPAERPPQLRPGAPTSLQTEPQDRSP
                     APMSCDKSTQTPSPPCQAFNHYLSAMASIRQSQEEPEDLRPEIRIAQELRRIGDEFNET
                     YTRRVFANDYREAEDHPQMVILQLLRFIFRLVWRRH"
     misc_signal     1587..1650
                     /label=BH3
                     /note="/SECDrawAs=Label"
     misc_feature    1762..1814
                     /gene="MCS"
                     /label=MCS
                     /note="/SECDrawAs=Region"
                     /note="/SECStyleId=1"
     misc_feature    1823..1833
                     /gene="StopCodons"
                     /label=StopCodons
                     /note="/SECDrawAs=Region"
                     /note="/SECStyleId=1"
     misc_feature    1831..2059
                     /gene="SV40_PA_terminator"
                     /label=SV40_PA_terminator
                     /note="/SECDrawAs=Region"
                     /note="/SECStyleId=1"
     misc_feature    complement(2198..2504)
                     /gene="f1 ori"
                     /label=f1 ori
                     /note="/SECDrawAs=Region"
                     /note="/SECStyleId=1"
     misc_feature    2583..2611
                     /gene="AmpR Promoter"
                     /label=AmpR Promoter
                     /note="/SECDrawAs=Region"
                     /note="/SECStyleId=1"
     misc_feature    2695..2963
                     /gene="SV40 Promoter"
                     /label=SV40 Promoter
                     /note="/SECDrawAs=Region"
                     /note="/SECStyleId=1"
     misc_feature    2862..2939
                     /gene="SV40 ori"
                     /label=SV40 ori
                     /note="/SECDrawAs=Region"
                     /note="/SECStyleId=1"
     CDS             3046..3837
                     /codon_start=1
                     /gene="NeoR/KanR"
                     /label=NeoR/KanR
                     /note="/SECDrawAs=Gene"
                     /note="/SECStyleId=1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    4015..4284
                     /gene="TK_PA_terminator"
                     /label=TK_PA_terminator
                     /note="/SECDrawAs=Region"
                     /note="/SECStyleId=1"
     misc_feature    4432..5051
                     /gene="pB322_ORI"
                     /label=pB322_ORI
                     /note="/SECDrawAs=Region"
                     /note="/SECStyleId=1"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
     1081 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga acgcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagg gaggctccat ggccaagcaa ccttctgatg taagttctga gtgtgacaga
     1381 gaaggtggac aattgcagcc tgctgagagg cctccccagc tcaggcctgg ggcccctacc
     1441 tccctacaga cagaaccgca agacaggagc ccggcaccca tgagttgtga caagtcaaca
     1501 caaaccccaa gtcctccttg ccaggccttc aaccactatc tcagtgcaat ggcttccata
     1561 cgacagtctc aggaggaacc tgaagatctg cgcccggaga tacggattgc acaggagctg
     1621 cggcggatcg gagacgagtt caacgaaact tacacaagga gggtgtttgc aaatgattac
     1681 cgcgaggctg aagaccaccc tcaaatggtt atcttacaac tgttacgctt tatcttccgt
     1741 ctggtatgga gaaggcatta actcgagctc aagcttcgaa ttctgcagtc gacggtaccg
     1801 cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag
     1861 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     1921 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     1981 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     2041 aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
     2101 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     2161 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     2221 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     2281 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     2341 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     2401 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     2461 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     2521 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2581 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2641 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     2701 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     2761 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     2821 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     2881 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     2941 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3001 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     3061 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     3121 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     3181 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     3241 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     3301 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     3361 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     3421 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     3481 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     3541 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     3601 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     3661 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     3721 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     3781 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     3841 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     3901 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     3961 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     4021 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     4081 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     4141 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     4201 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     4261 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
     4321 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4381 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4441 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4501 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4561 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     4621 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4681 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4741 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4801 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4861 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4921 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4981 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5041 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     5101 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
