LOCUS       mC3-BclX-s2193          4855 bp    DNA     circular SYN 27-MAY-2021
DEFINITION  
ACCESSION   mC3-BclX-s2193
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    23..253
                     /gene="sV40 pAn"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    224..1007
                     /gene="pMB1 Ori"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1008..1248
                     /gene="SV40 enh"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1249..2356
                     /gene="hFerH promoter + mEF1 5' UTR"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             2387..3103
                     /gene="mCerulean3"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2387..3826
                     /gene="frame"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKN
                     GIKANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKSGLRSREMSQSNRELVVDFLSYKLSQKGYSWSQFSDV
                     EENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQ
                     ALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSF
                     GGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESR
                     KGQERFNRWFLTGMTVAGVVLLGSLFSRK"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             3104..3124
                     /gene="Linker 7aa"
                     /translation="SGLRSRE"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             3125..3826
                     /gene="BclXL"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    3880..4348
                     /gene="IRES"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4349..4423
                     /gene="EM7"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             4424..4848
                     /gene="BlasticidinR"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 cttcgaactc gaggctagct ggccagacat gataagatac attgatgagt ttggacaaac
       61 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
      121 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
      181 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
      241 tggtatggaa atgttaatta actagccatg accaaaatcc cttaacgtga gttttcgttc
      301 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
      361 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
      421 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      481 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      541 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      601 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      661 acgggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
      721 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      781 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      841 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      901 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      961 tggccttttg ctggcctttt gctcacatgt tcttaattaa cctgcagggc ctgaaataac
     1021 ctctgaaaga ggaacttggt taggtacctt ctgaggctga aagaaccagc tgtggaatgt
     1081 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     1141 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     1201 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccactagttc cgccagagcg
     1261 cgcgagggcc tccagcggcc gcccctcccc cacagcaggg gcggggtccc gcgcccaccg
     1321 gaaggagcgg gctcggggcg ggcggcgctg attggccggg gcgggcctga cgccgacgcg
     1381 gctataagag accacaagcg acccgcaggg ccagacgttc ttcgccgaag cttgccgtca
     1441 gaacgcaggt gaggggcggg tgtggcttcc gcgggccgcc gagctggagg tcctgctccg
     1501 agcgggccgg gccccgctgt cgtcggcggg gattagctgc gagcattccc gcttcgagtt
     1561 gcgggcggcg cgggaggcag agtgcgaggc ctagcggcaa ccccgtagcc tcgcctcgtg
     1621 tccggcttga ggcctagcgt ggtgtccgcg ccgccgccgc gtgctactcc ggccgcactc
     1681 tggtcttttt tttttttgtt gttgttgccc tgctgccttc gattgccgtt cagcaatagg
     1741 ggctaacaaa gggagggtgc ggggcttgct cgcccggagc ccggagaggt catggttggg
     1801 gaggaatgga gggacaggag tggcggctgg ggcccgcccg ccttcggagc acatgtccga
     1861 cgccacctgg atggggcgag gcctggggtt tttcccgaag caaccaggct ggggttagcg
     1921 tgccgaggcc atgtggcccc agcacccggc acgatctggc ttggcggcgc cgcgttgccc
     1981 tgcctcccta actagggtga ggccatcccg tccggcacca gttgcgtgcg tggaaagatg
     2041 gccgctcccg ggccctgttg caaggagctc aaaatggagg acgcggcagc ccggtggagc
     2101 gggcgggtga gtcacccaca caaaggaaga gggcctggtc cctcaccggc tgctgcttcc
     2161 tgtgaccccg tggtcctatc ggccgcaata gtcacctcgg gcttttgagc acggctagtc
     2221 gcggcggggg gaggggatgt aatggcgttg gagtttgttc acatttggtg ggtggagact
     2281 agtcaggcca gcctggcgct ggaagtcatt tttggaattt gtccccttga gttttgagcg
     2341 gagctaattc aaagcaacgg gtgatctagc gctaccggtc gccaccatgg tgagcaaggg
     2401 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     2461 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     2521 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     2581 gagctggggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
     2641 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     2701 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     2761 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     2821 cgccatccac ggcaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
     2881 cttcggcctc aactgcaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     2941 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     3001 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     3061 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatctcg
     3121 agaaatgagc caaagcaacc gggagctggt ggttgacttt ctctcctaca agctttccca
     3181 gaaaggatac agctggagtc agtttagtga tgtggaagag aacaggactg aggccccaga
     3241 agggactgaa tcggagatgg agacccccag tgccatcaat ggcaacccat cctggcacct
     3301 ggcagacagc cccgcggtga atggagccac tggccacagc agcagtttgg atgcccggga
     3361 ggtgatcccc atggcagcag taaagcaagc gctgagggag gcaggcgacg agtttgaact
     3421 gcggtaccgg cgggcattca gtgacctgac atcccagctc cacatcaccc cagggacagc
     3481 atatcagagc tttgaacagg tagtgaatga actcttccgg gatggggtaa actggggtcg
     3541 cattgtggcc tttttctcct tcggcggggc actgtgcgtg gaaagcgtag acaaggagat
     3601 gcaggtattg gtgagtcgga tcgcagcttg gatggccact tacctgaatg accacctaga
     3661 gccttggatc caggagaacg gcggctggga tacttttgtg gaactctatg ggaacaatgc
     3721 agcagccgag agccgaaagg gccaggaacg cttcaaccgc tggttcctga cgggcatgac
     3781 tgtggccggc gtggttctgc tgggctcact cttcagtcgg aaatgaagat ccaccggatc
     3841 tagataactg atccacgcgt atcgattgtc gaccctagga gcaggtttcc ccaatgacac
     3901 aaaacgtgca acttgaaact ccgcctggtc tttccaggtc tagaggggta acactttgta
     3961 ctgcgtttgg ctccacgctc gatccactgg cgagtgttag taacagcact gttgcttcgt
     4021 agcggagcat gacggccgtg ggaactcctc cttggtaaca aggacccacg gggccaaaag
     4081 ccacgcccac acgggcccgt catgtgtgca accccagcac ggcgacttta ctgcgaaacc
     4141 cactttaaag tgacattgaa actggtaccc acacactggt gacaggctaa ggatgccctt
     4201 caggtacccc gaggtaacac gcgacactcg ggatctgaga aggggactgg ggcttctata
     4261 aaagcgctcg gtttaaaaag cttctatgcc tgaataggtg accggaggtc ggcacctttc
     4321 ctttgcaatt actgacccta tgaatacaac tgactgtttg acaattaatc atcggcatag
     4381 tatatcggca tagtataata cgactcacta taggagggcc accatgaaga ccttcaacat
     4441 ctctcagcag gatctggagc tggtggaggt cgccactgag aagatcacca tgctctatga
     4501 ggacaacaag caccatgtcg gggcggccat caggaccaag actggggaga tcatctctgc
     4561 tgtccacatt gaggcctaca ttggcagggt cactgtctgt gctgaagcca ttgccattgg
     4621 gtctgctgtg agcaacgggc agaaggactt tgacaccatt gtggctgtca ggcaccccta
     4681 ctctgatgag gtggacagat ccatcagggt ggtcagcccc tgtggcatgt gcagagagct
     4741 catctctgac tatgctcctg actgctttgt gctcattgag atgaatggca agctggtcaa
     4801 aaccaccatt gaggaactca tccccctcaa gtacaccagg aactaaacct gaatt
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