LOCUS Exported 6798 bp ds-DNA circular SYN 02-FEB-2021 DEFINITION pETcon yeast surface display vector, expressing the SARS-2 RBD 201aa construct. ACCESSION urn.local...34muarj-4v VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6798) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, Feb 2, 2021 from SnapGene Viewer 5.2.4 https://www.snapgene.com COMMENT 2/2020 Tyler N Starr Constructed from p2439 It contains a tryptophan selective marker for complementation of the trp1 mutation FEATURES Location/Qualifiers source 1..6798 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 64..80 /locus_tag="T3" /label=T3 promoter 105..707 /locus_tag="Bidirectional promoter" /label=Bidirectional promoter /note="Bidirectional promoter" 5'UTR complement(105..112) /locus_tag="Partial" /label=Partial /note="Partial" promoter complement(112..470) /locus_tag="promoter" /label=promoter misc_feature 112 /locus_tag="Geneious type: misc_marker(1)" /label=Geneious type: misc_marker /label=Geneious type: misc_marker(1) /note="Geneious type: misc_marker" /note="Transcription Start (first base of mRNA)" promoter 496..707 /locus_tag="GAL1 minimal promoter" /label=GAL1 minimal promoter /note="GAL1 minimal promoter" 5'UTR 707..763 /locus_tag="Partial (all but the last 4 bases)" /label=Partial (all but the last 4 bases) /note="Partial (all but the last 4 bases)" misc_feature 707 /locus_tag="Geneious type: misc_marker" /label=Geneious type: misc_marker /note="Geneious type: misc_marker" /note="Transcription start (first base of mRNA)" primer_bind 727..750 /locus_tag="Gal1seq" /label=Gal1seq misc_feature 772..777 /locus_tag="EcoRI" /label=EcoRI 5'UTR 779..802 /locus_tag="5'UTR" /label=5'UTR CDS 803..1063 /locus_tag="CDS (Aga2p)" /label=CDS (Aga2p) misc_feature 1065..1089 /locus_tag="primer: o4 Aga2p_fwd" /label=primer: o4 Aga2p_fwd /note="Geneious type: primer" CDS 1097..1132 /locus_tag="CDS (HA tag)" /label=CDS (HA tag) CDS 1133..1177 /locus_tag="CDS (GGS linker)" /label=CDS (GGS linker) misc_feature 1155..1162 /locus_tag="NotI" /label=NotI misc_binding 1184..1189 /locus_tag="NdeI" /label=NdeI misc_feature 1190..1792 /locus_tag="RBD-201aa" /label=RBD-201aa misc_binding 1793..1798 /locus_tag="XhoI" /label=XhoI CDS 1799..1810 /locus_tag="CDS" /label=CDS CDS 1811..1840 /locus_tag="CDS (Myc tag)" /label=CDS (Myc tag) 3'UTR 1851..2155 /locus_tag="yeast mating factor alpha 3' UTR" /label=yeast mating factor alpha 3' UTR /note="yeast mating factor alpha 3' UTR" misc_feature 2156..2161 /locus_tag="SacI" /label=SacI primer_bind complement(2169..2188) /locus_tag="T7" /label=T7 misc_feature 2208..2241 /locus_tag="Illumina P7 handle" /label=Illumina P7 handle rep_origin 2386..2841 /locus_tag="rep_origin" /label=rep_origin misc_feature 2511..2516 /locus_tag="NaeI" /label=NaeI 3'UTR complement(2898..2943) /locus_tag="3'UTR" /label=3'UTR CDS complement(2944..3618) /locus_tag="Tyrosinase-related protein 1 (Yeast Selectablemarker)" /label=Tyrosinase-related protein 1 (Yeast Selectable ... /label=Tyrosinase-related protein 1 (Yeast Selectablemarker) /note="Tyrosinase-related protein 1 (Yeast Selectablemarker)" 5'UTR complement(3619..3722) /locus_tag="5'UTR(1)" /label=5'UTR(1) rep_origin complement(4151..4664) /direction=LEFT /locus_tag="rep_origin(1)" /label=rep_origin(1) rep_origin complement(4155..4531) /direction=LEFT /locus_tag="rep_origin(2)" /label=rep_origin(2) centromere 4544..4569 /label=CDEI fragment of chromosome VI centromere from ... /label=CDEI fragment of chromosome VI centromere from S.cerevisiae /note="CDEI fragment of chromosome VI centromere from S.cerevisiae" /note="/locus_tag=CDEI fragment of chromosome VI centromere from S.cerevisiae" promoter 4733..4761 /locus_tag="promoter(1)" /label=promoter(1) CDS 4803..5663 /locus_tag="CDS(1)" /label=CDS(1) rep_origin 5811..6596 /locus_tag="rep_origin(3)" /label=rep_origin(3) ORIGIN 1 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc 61 gaaattaacc ctcactaaag ggaacaaaag ctggtaccaa ttccttgaat tttcaaaaat 121 tcttactttt tttttggatg gacgcaaaga agtttaataa tcatattaca tggcattacc 181 accatataca tatccatatc taatcttact tatatgttgt ggaaatgtaa agagccccat 241 tatcttagcc taaaaaaacc ttctctttgg aactttcagt aatacgctta actgctcatt 301 gctatattga agtacggatt agaagccgcc gagcgggtga cagccctccg aaggaagact 361 ctcctccgtg cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg tgcctcgcgc 421 cgcactgctc cgaacaataa agattctaca atactagctt ttatggttat gaagaggaaa 481 aattggcagt aacctggccc cacaaacctt caaatgaacg aatcaaatta acaaccatag 541 gatgataatg cgattagttt tttagcctta tttctggggt aattaatcag cgaagcgatg 601 atttttgatc tattaacaga tatataaatg caaaaactgc ataaccactt taactaatac 661 tttcaacatt ttcggtttgt attacttctt attcaaatgt aataaaagta tcaacaaaaa 721 attgttaata tacctctata ctttaacgtc aaggagaaaa aaccccggat cgaattccct 781 acttcataca ttttcaatta agatgcagtt acttcgctgt ttttcaatat tttctgttat 841 tgcttcagtt ttagcacagg aactgacaac tatatgcgag caaatcccct caccaacttt 901 agaatcgacg ccgtactctt tgtcaacgac tactattttg gccaacggga aggcaatgca 961 aggagttttt gaatattaca aatcagtaac gtttgtcagt aattgcggtt ctcacccctc 1021 aacaactagc aaaggcagcc ccataaacac acagtatgtt tttaaggaca atagctcgac 1081 gattgaaggt agatacccat acgacgttcc agactacgct ctgcaggcta gtggtggagg 1141 aggctctggt ggaggcggcc gcggaggcgg agggtcggct agccatatga atatcacgaa 1201 cctttgtcct ttcggtgagg tcttcaatgc tactagattc gcatccgtgt atgcatggaa 1261 tagaaagaga attagtaatt gtgtagcgga ctactctgta ctttataact ccgcctcctt 1321 ctccacattc aagtgttacg gtgtatctcc caccaagttg aatgatctat gctttacaaa 1381 cgtttacgcc gatagtttcg taattagagg cgatgaagtg cgtcagatcg caccaggcca 1441 gacgggcaag atagcagact ataattataa gctgcctgat gacttcaccg gctgtgtgat 1501 agcttggaac tcaaataatc tagattccaa ggtgggaggc aattacaatt atttgtaccg 1561 tctgttccgt aaaagcaatt tgaaaccatt tgaaagagac attagcactg aaatttatca 1621 agcagggtcc accccgtgca acggcgtaga aggctttaac tgttatttcc cattacagtc 1681 ttatggtttc caacctacga acggagtcgg gtatcagccg tacagggttg tggttctttc 1741 atttgaactg ctgcacgcgc ccgcaaccgt atgcgggccg aagaaatcaa cgctcgaggg 1801 gggcggttcc gaacaaaagc ttatttctga agaggacttg taatagagat ctgataacaa 1861 cagtgtagat gtaacaaaat cgactttgtt cccactgtac ttttagctcg tacaaaatac 1921 aatatacttt tcatttctcc gtaaacaaca tgttttccca tgtaatatcc ttttctattt 1981 ttcgttccgt taccaacttt acacatactt tatatagcta ttcacttcta tacactaaaa 2041 aactaagaca attttaattt tgctgcctgc catatttcaa tttgttataa attcctataa 2101 tttatcctat tagtagctaa aaaaagatga atgtgaatcg aatcctaaga gaattgagct 2161 ccaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgttaga tcggaagagc 2221 acacgtctga actccagtca cttacaacgt cgtgactggg aaaaccctgg cgttacccaa 2281 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 2341 accgatcgcc tttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg 2401 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 2461 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 2521 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 2581 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 2641 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 2701 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 2761 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 2821 aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat 2881 ttcacaccgc atagatcggc aagtgcacaa acaatactta aataaatact actcagtaat 2941 aacctatttc ttagcatttt tgacgaaatt tgctattttg ttagagtctt ttacaccatt 3001 tgtctccaca cctccgctta catcaacacc aataacgcca tttaatctaa gcgcatcacc 3061 aacattttct ggcgtcagtc caccagctaa cataaaatgt aagctttcgg ggctctcttg 3121 ccttccaacc cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc cacctgcttc 3181 tgaatcaaac aagggaataa acgaatgagg tttctgtgaa gctgcactga gtagtatgtt 3241 gcagtctttt ggaaatacga gtcttttaat aactggcaaa ccgaggaact cttggtattc 3301 ttgccacgac tcatctccat gcagttggac gatatcaatg ccgtaatcat tgaccagagc 3361 caaaacatcc tccttaggtt gattacgaaa cacgccaacc aagtatttcg gagtgcctga 3421 actattttta tatgctttta caagacttga aattttcctt gcaataaccg ggtcaattgt 3481 tctctttcta ttgggcacac atataatacc cagcaagtca gcatcggaat ctagagcaca 3541 ttctgcggcc tctgtgctct gcaagccgca aactttcacc aatggaccag aactacctgt 3601 gaaattaata acagacatac tccaagctgc ctttgtgtgc ttaatcacgt atactcacgt 3661 gctcaatagt caccaatgcc ctccctcttg gccctctcct tttctttttt cgaccgaatt 3721 aattcttaat cggcaaaaaa agaaaagctc cggatcaaga ttgtacgtaa ggtgacaagc 3781 tatttttcaa taaagaatat cttccactac tgccatctgg cgtcataact gcaaagtaca 3841 catatattac gatgctgttc tattaaatgc ttcctatatt atatatatag taatgtcgtg 3901 atctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac 3961 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca 4021 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga 4081 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa 4141 taatggtttc ttagacggat cgcttgcctg taacttacac gcgcctcgta tcttttaatg 4201 atggaataat ttgggaattt actctgtgtt tatttatttt tatgttttgt atttggattt 4261 tagaaagtaa ataaagaagg tagaagagtt acggaatgaa gaaaaaaaaa taaacaaagg 4321 tttaaaaaat ttcaacaaaa agcgtacttt acatatatat ttattagaca agaaaagcag 4381 attaaataga tatacattcg attaacgata agtaaaatgt aaaatcacag gattttcgtg 4441 tgtggtcttc tacacagaca agatgaaaca attcggcatt aatacctgag agcaggaaga 4501 gcaagataaa aggtagtatt tgttggcgat ccccctagag tcttttacat cttcggaaaa 4561 caaaaactat tttttcttta atttcttttt ttactttcta tttttaattt atatatttat 4621 attaaaaaat ttaaattata attattttta tagcacgtga tgaaaaggac ccaggtggca 4681 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 4741 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 4801 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 4861 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 4921 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 4981 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 5041 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 5101 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 5161 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 5221 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 5281 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 5341 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 5401 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 5461 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 5521 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 5581 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 5641 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 5701 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 5761 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 5821 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 5881 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 5941 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 6001 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 6061 tcnsarscvr bdaagbtacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 6121 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 6181 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 6241 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 6301 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 6361 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 6421 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 6481 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 6541 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 6601 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 6661 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 6721 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg 6781 gctcgtatgt tgtgtgga //