LOCUS       Exported                3089 bp ds-DNA     circular SYN 04-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 4, 2021 from SnapGene Viewer 5.2.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        7..247
                     /label=U6 promoter
     primer_bind     7..27
                     /label=hU6-F
     primer_bind     178..197
                     /label=LKO.1 5'
     misc_feature    257..292
                     /label=CasRx DR (full)
     misc_feature    293..322
                     /label=30nt VEGFA 1 pre-sgRNA
     misc_feature    323..358
                     /label=CasRx DR (full)
     misc_feature    359..388
                     /label=30nt VEGFA 2 pre-sgRNA
     misc_feature    389..424
                     /label=CasRx DR (full)
     misc_feature    425..454
                     /label=30nt VEGFA 3 pre-sgRNA
     misc_feature    455..490
                     /label=CasRx DR (full)
     rep_origin      572..1027
                     /label=f1 ori
     primer_bind     complement(659..678)
                     /label=F1ori-R
     primer_bind     869..890
                     /label=F1ori-F
     primer_bind     complement(1044..1063)
                     /label=pRS-marker
     primer_bind     1163..1185
                     /label=pGEX 3'
     primer_bind     complement(1223..1241)
                     /label=pBRforEco
     promoter        1309..1413
                     /label=AmpR promoter
     CDS             1414..2274
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1632..1651)
                     /label=Amp-R
     rep_origin      2445..3033
                     /label=ori
     primer_bind     2934..2953
                     /label=pBR322ori-F
ORIGIN
        1 gctagcgagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
       61 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
      121 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
      181 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
      241 aaaggacgaa acaccgcaag taaaccccta ccaactggtc ggggtttgaa actatgtgct
      301 ggccttggtg aggtttgatc cgcaagtaaa cccctaccaa ctggtcgggg tttgaaacca
      361 cttcgtgatg attctgccct cctccttcca agtaaacccc taccaactgg tcggggtttg
      421 aaactctgca ttcacatttg ttgtgctgta ggaacaagta aacccctacc aactggtcgg
      481 ggtttgaaac tttttttgaa ttctgatgcg gtattttctc cttacgcatc tgtgcggtat
      541 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
      601 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
      661 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
      721 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
      781 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
      841 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
      901 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
      961 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     1021 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     1081 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     1141 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     1201 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     1261 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     1321 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     1381 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     1441 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     1501 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     1561 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     1621 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     1681 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     1741 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1801 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1861 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     1921 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     1981 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     2041 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     2101 gctgataaat ctggagccgg tgagcgtgga agccgcggta tcattgcagc actggggcca
     2161 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     2221 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     2281 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     2341 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     2401 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2461 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2521 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2581 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2641 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2701 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2761 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2821 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2881 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2941 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3001 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3061 ttcctggcct tttgctggcc ttttgctca
//
