LOCUS       Exported               13138 bp ds-DNA     circular SYN 07-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13138)
  AUTHORS   Friedel Lab
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Feb 7, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13138
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(1..18)
                     /label=pQE-F
     primer_bind     complement(1..17)
                     /label=QE_Promoter
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2075..2105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2119..2140
                     /label=M13R
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     primer_bind     2174..2193
                     /label=T3a
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2672..2797
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3290..3523
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4019..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4158..4472
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4166..4184
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4202..4220
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4237..4255
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4273..4291
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4309..4327
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4344..4362
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4380..4398
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4410..4419
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4445..4469
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     4451..4469
                     /label=pEGFP-FP
     protein_bind    4550..4574
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(4558..4574)
                     /label=attL1
     primer_bind     4591..4611
                     /label=TET3-entry-F
     misc_feature    4595..9769
                     /label=TET3 CDS
                     /note="note=""direct strand 
                     translation""translation=""MDYKDDDDKLDGGYPYDVPDYAGGLDSQFQVP
                     LAVQPDLPGLYDFPQRQVMVGSFPGSGLSMAGSESQLRGGGDGRKKRKRCGTCEPCRRL
                     ENCGACTSCTNRRTHQICKLRKCEVLKKKVGLLKEVEIKAGEGAGPWGQGAAVKTGSEL
                     SPVDGPVPGQMDSGPVYHGDSRQLSASGVPVNGAREPAGPSLLGTGGPWRVDQKPDWEA
                     "
     CDS             4595..9766
                     /label=ORF frame 2
                     /note="label=""ORF frame 2"" - 
                     translation=""MDYKDDDDKLDGGYPYDVPDYAGGLDSQFQVPLAVQPDLPGLYD 
                     - 
                     FPQRQVMVGSFPGSGLSMAGSESQLRGGGDGRKKRKRCGTCEPCRRLENCGACTSCTN 
                     - 
                     RRTHQICKLRKCEVLKKKVGLLKEVEIKAGEGAGPWGQGAAVKTGSELSPVDGPVPGQ 
                     - MDSGPVYHGDSRQLSASGVPVNGAREPAGPSLLGTGGPWRVDQKPDWEAAP"
     misc_feature    4598..4621
                     /label=FLAG
                     /note="label=""FLAG"" - 
                     translation=""MDYKDDDDKLDGGYPYDVPDYAGGLDSQFQVPLAVQPDLPGLYD 
                     - 
                     FPQRQVMVGSFPGSGLSMAGSESQLRGGGDGRKKRKRCGTCEPCRRLENCGACTSCTN 
                     - 
                     RRTHQICKLRKCEVLKKKVGLLKEVEIKAGEGAGPWGQGAAVKTGSELSPVDGPVPGQ 
                     - 
                     MDSGPVYHGDSRQLSASGVPVNGAREPAGPSLLGTGGPWRVDQKPDWEAAPGPAHTAR"
     misc_feature    4634..4660
                     /label=HA
                     /note="label=""HA"" - 
                     translation=""MDYKDDDDKLDGGYPYDVPDYAGGLDSQFQVPLAVQPDLPGLYD 
                     - 
                     FPQRQVMVGSFPGSGLSMAGSESQLRGGGDGRKKRKRCGTCEPCRRLENCGACTSCTN 
                     - 
                     RRTHQICKLRKCEVLKKKVGLLKEVEIKAGEGAGPWGQGAAVKTGSELSPVDGPVPGQ 
                     - 
                     MDSGPVYHGDSRQLSASGVPVNGAREPAGPSLLGTGGPWRVDQKPDWEAAPGPAHTAR 
                     -"
     CDS             complement(7088..8302)
                     /label=ORF frame 1
                     /note="label=""ORF frame 1"" - 
                     translation=""MPPGTRTQELRGEDGVAGWKAIVAKRRESVLGVEAIDGGQAGLC 
                     - 
                     IVGVVGVHAVHAVRVGGPAVAQHRVALHHRVAAVLEGAEVVLRLHLEGGLQALLGQST 
                     - 
                     LQRQAWVRRNARQLQGLLLDLLRSAQLLLLNLLLLGCCLSGFQLPLAAGLGRLGQASD 
                     - LAGEGGEHLDGSAAHLCILVLLATKLIRVGHLVQGENMQLLILGNLAHAAI"
     CDS             complement(8674..9444)
                     /label=ORF frame 2
                     /note="label=""ORF frame 2"" - 
                     translation=""MRVGWQRLGFLSGVVACSSRRAHSMRMEPWAGATATPPMFSSRK 
                     - 
                     LCSLSDHSSSSSSSAPPPPFSSFTAPLLGGSSAGPSSRLKGSHSFSSDFRAPNSFSLD 
                     - 
                     PRGRPKDCQALSSWLAPAAGILPSSPFMGFHSLSWNPGLPFRAELKSPAPSAHGFSVR 
                     - LGPAKAEVLFPNLQGLHGLPRSRPPEWEAACCPSPGNSPHWPSVKWEGARQ"
     CDS             complement(9170..9769)
                     /label=ORF frame 1
                     /note="label=""ORF frame 1"" - 
                     translation=""MVMVMMTGTRRIETEERVRDRLTFEGPSRLDPAAVGASDLRVGI 
                     - 
                     GGHGDRRVCGHSQCLPGGDDPLLLFLLGLSNHSAPPLALLPVELGLLLAQAQPGSLLL 
                     - 
                     PCPCPLRQLLHLGFPEGQPVVGLVEVLVLVEDQRDAGGVAAVGLLKRRGGVQLPPGTL 
                     - DEDGAVGWGHGHAADVLVQEVVFTVRPQLLLFLLRTTTALLLLHRSLAGGL"
     primer_bind     complement(9749..9769)
                     /label=TET3-entry-R
     protein_bind    complement(9786..9810)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9786..9795
                     /label=attL2
     misc_feature    9841..10429
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9894..9914)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10312..10323)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        10472..10971
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10507..10530)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(10742..10753)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     primer_bind     10904..10923
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10985..11788
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11048..11067)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11658..11677
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             11879..12112
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12184..12305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12221..12240)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12275..12294
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12324..12459
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12386..12405
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(12409..12420)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(12491..12510)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12492..12510)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12493..12509)
                     /label=T7a
     primer_bind     complement(12520..12537)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12520..12536)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12520..12536)
                     /label=M13F
     primer_bind     complement(12529..12551)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12538..12556)
                     /label=U-19mer_Primer
     primer_bind     complement(12540..12556)
                     /label=M13F-pUC
     rep_origin      12681..13136
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12768..12787)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12978..12999
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     4141 atcacgagac tagcctcgag tttactccct atcagtgata gagaacgtat gtcgagttta
     4201 ctccctatca gtgatagaga acgatgtcga gtttactccc tatcagtgat agagaacgta
     4261 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     4321 atagagaacg tatgtcgagt ttatccctat cagtgataga gaacgtatgt cgagtttact
     4381 ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt acggtgggag gcctatataa
     4441 gcagagctcg tttagtgaac cgtcagatcg cctggagaat tcgagctcgg tacccgggga
     4501 tccaccggtt cgtcgactag tccagtgtgg tggaattctg cagatatcaa caagtttgta
     4561 caaaaaagca ggctccgcgg ccgccccctt caccatggac tacaaggacg acgatgacaa
     4621 gctcgatgga ggatacccct acgacgtgcc cgactacgcc caccagagga ggccagccga
     4681 ggtggaaata aaggctggtg aaggagccgg gccgtgggga caaggagcgg ctgtcaagac
     4741 aggctcagag ctcagcccag ttgatggacc tgttccaggt cagatggact cagggccagt
     4801 gtaccatggg gactcacggc agctaagcgc ctcaggggtg ccggtcaatg gtgctagaga
     4861 gcccgctgga cccagtctgc tggggactgg gggtccttgg cgggtagacc aaaagcccga
     4921 ctgggaggct gccccaggcc cagctcatac tgctcgcctg gaagatgccc acgatctggt
     4981 ggccttttcg gctgtggccg aagctgtgtc ctcttatggg gcccttagca cccggctcta
     5041 tgaaaccttc aaccgtgaga tgagtcgtga ggctgggaac aacagcaggg gaccccggcc
     5101 agggcctgag ggctgctctg ctggcagcga agaccttgac acactgcaga cggccctggc
     5161 cctcgcgcgg catggtatga aaccacccaa ctgcaactgc gatggcccag aatgccctga
     5221 ctacctcgag tggctggagg ggaagatcaa gtctgtggtc atggaaggag gggaggagcg
     5281 gcccaggctc ccagggcctc tgcctcctgg tgaggccggc ctcccagcac caagcaccag
     5341 gccactcctc agctcagagg tgccccagat ctctccccaa gagggcctgc ccctgtccca
     5401 gagtgccctg agcattgcca aggaaaaaaa catcagcttg cagaccgcca ttgccattga
     5461 ggccctcaca cagctctcct ctgccctccc gcagccttct cattccaccc cccaggcttc
     5521 ttgccccctt cctgaggcct tgtcacctcc tgcccctttc agatctcccc agtcttacct
     5581 ccgggctccc tcatggcctg tggttcctcc tgaagagcac tcatcttttg ctcctgatag
     5641 ctctgccttc cctccagcaa ctcctagaac tgagttccct gaagcctggg gcactgacac
     5701 ccctccagca acgccccgga gctcctggcc catgcctcgc ccaagccccg atcccatggc
     5761 tgaactggag cagttgttgg gcagcgccag tgattacatc cagtcagtat tcaagcggcc
     5821 tgaggccctg cctaccaagc ccaaggtcaa ggtggaggca ccctcttcct ccccggcccc
     5881 ggccccatcc cctgtacttc agagggaggc tcccacgcca tcctcggagc ccgacaccca
     5941 ccagaaggcc cagaccgccc tgcagcagca cctccaccac aagcgcagcc tcttcctaga
     6001 acaggtgcac gacacctcct tccctgctcc ttcagagcct tctgctcctg gctggtggcc
     6061 cccaccaagt tcacctgtcc cacggcttcc agacagacca cccaaggaga agaagaagaa
     6121 gctcccaaca ccagctggag gtcccgtggg aacggagaaa gctgcccctg ggatcaagcc
     6181 cagtgtccga aagcccattc agatcaagaa gtccaggccc cgggaagcac agcccctctt
     6241 cccacctgtc cgacagattg tcctggaagg gcttaggtcc ccagcctccc aggaagtgca
     6301 ggctcatcca ccggcccctc tgcctgcctc acagggctct gctgtgcccc tgcccccaga
     6361 accttctctt gcgctatttg cacctagtcc ctccagggac agcctgctgc cccctactca
     6421 ggaaatgagg tcccccagcc ccatgacagc cttgcagcca ggctccactg gccctcttcc
     6481 ccctgccgat gacaagctgg aagagctcat ccggcagttt gaggctgaat ttggagatag
     6541 ctttgggctt cccggccccc cttctgtgcc cattcaggac cccgagaacc agcaaacatg
     6601 tctcccagcc cctgagagcc cctttgctac ccgttccccc aagcaaatca agattgagtc
     6661 ttcgggggct gtgactgtgc tctcaaccac ctgcttccat tcagaggagg gaggacagga
     6721 ggccacaccc accaaggctg agaacccact cacacccacc ctcagtggct tcttggagtc
     6781 acctcttaag tacctggaca cacccaccaa gagtctgctg gacacacctg ccaagagagc
     6841 ccaggccgag ttccccacct gcgattgcgt cgaacaaata gtggagaaag atgaaggtcc
     6901 atattatact cacttgggat ctggccccac ggtcgcctct atccgggaac tcatggagga
     6961 gcggtatgga gagaagggga aagccatccg gatcgagaag gtcatctaca cggggaagga
     7021 gggaaagagc tcccgcggtt gccccattgc aaagtgggtg atccgcaggc acacgctgga
     7081 ggagaagcta ctctgcctgg tgcggcaccg ggcaggccac cactgccaga acgctgtgat
     7141 cgtcatcctc atcctggcct gggagggcat tccccgtagc ctcggagaca ccctctacca
     7201 ggagctcacc gacaccctcc ggaagtatgg gaaccccacc agccggagat gcggcctcaa
     7261 cgatgaccgg acctgcgctt gccaaggcaa agaccccaac acctgtggtg cctccttctc
     7321 ctttggttgt tcctggagca tgtacttcaa cggctgcaag tatgctcgga gcaagacacc
     7381 tcgcaagttc cgcctcgcag gggacaatcc caaagaggaa gaagtgctcc ggaagagttt
     7441 ccaggacctg gccaccgaag tcgctcccct gtacaagcga ctggcccctc aggcctatca
     7501 gaaccaggtg accaacgagg aaatagcgat tgactgccgt ctggggctga aggaaggacg
     7561 gcccttcgcg ggggtcacgg cctgcatgga cttctgtgcc cacgcccaca aggaccagca
     7621 taacctctac aatgggtgca ccgtggtctg caccctgacc aaggaagaca atcgctgcgt
     7681 gggcaagatt cccgaggatg agcagctgca tgttctcccc ctgtacaaga tggccaacac
     7741 ggatgagttt ggtagcgagg agaaccagaa tgcaaaggtg ggcagcggag ccatccaggt
     7801 gctcaccgcc ttcccccgcg aggtccgacg cctgcccgag cctgccaagt cctgccgcca
     7861 gcggcagctg gaagccagaa aggcagcagc cgagaagaag aagattcaga aggagaagct
     7921 gagcactccg gagaagatca agcaggaggc cctggagctg gcgggcatta cgtcggaccc
     7981 aggcctgtct ctgaagggtg gattgtccca gcaaggcctg aagccctccc tcaaggtgga
     8041 gccgcagaac cacttcagct ccttcaagta cagcggcaac gcggtggtgg agagctactc
     8101 ggtgctgggc aactgccggc cctccgaccc ttacagcatg aacagcgtgt actcctacca
     8161 ctcctactat gcacagccca gcctgacctc cgtcaatggc ttccactcca agtacgctct
     8221 cccgtctttt agctactatg gctttccatc cagcaacccc gtcttcccct ctcagttcct
     8281 gggtcctggt gcctgggggc atagtggcag cagtggcagt tttgagaaga agccagacct
     8341 ccacgctctg cacaacagcc tgagcccggc ctacggtggt gctgagtttg ccgagctgcc
     8401 cagccaggct gttcccacag acgcccacca ccccactcct caccaccagc agcctgcgta
     8461 cccaggcccc aaggagtatc tgcttcccaa ggccccccta ctccactcag tgtccaggga
     8521 cccctccccc tttgcccaga gctccaactg ctacaacaga tccatcaagc aagagccagt
     8581 agacccgctg acccaggctg agcctgtgcc cagagacgct ggcaagatgg gcaagacacc
     8641 tctgtccgag gtgtctcaga atggaggacc cagtcacctt tggggacagt actcaggagg
     8701 cccaagcatg tcccccaaga ggactaacgg tgtgggtggc agctggggtg tgttctcgtc
     8761 tggggagagt cctgccatcg tccctgacaa gctcagttcc tttggggcca gctgcctggc
     8821 cccttcccac ttcacagatg gccagtgggg gctgttcccc ggtgaggggc agcaggcagc
     8881 ttcccactct ggaggacggc tgcgaggcaa accgtggagc ccctgcaagt ttgggaacag
     8941 cacctcggcc ttggctgggc ccagcctgac tgagaagccg tgggcgctgg gggcagggga
     9001 tttcaactcg gccctgaaag gtagtcctgg gttccaagac aagctgtgga accccatgaa
     9061 aggagaggag ggcaggattc cagccgcagg ggccagccag ctggacaggg cctggcagtc
     9121 ctttggtctg cccctgggat ccagcgagaa gctgtttggg gctctgaagt cagaggagaa
     9181 gctgtgggac cccttcagcc tggaggaggg gccggctgag gagcccccca gcaagggagc
     9241 ggtgaaggag gagaagggcg gtggtggtgc ggaggaggaa gaggaggagc tgtggtcgga
     9301 cagtgaacac aacttcctgg acgagaacat cggcggcgtg gccgtggccc cagcccacgg
     9361 ctccatcctc atcgagtgtg cccggcggga gctgcacgcc accacgccgc ttaagaagcc
     9421 caaccgctgc caccccaccc gcatctcgct ggtcttctac cagcacaaga acctcaacca
     9481 gcccaaccac gggctggccc tctgggaagc caagatgaag cagctggcgg agagggcacg
     9541 ggcacggcag gaggaggctg cccggctggg cctgggccag caggaggcca agctctacgg
     9601 gaagaagcgc aagtgggggg gcactgtggt tgctgagccc cagcagaaag agaagaaggg
     9661 ggtcgtcccc acccggcagg cactggctgt gcccacagac tcggcggtca ccgtgtcctc
     9721 ctatgcctac acgaaggtca ctggccccta cagccgctgg atctagtcta agggtgggcg
     9781 cgccgaccca gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctcgac
     9841 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     9901 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     9961 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
    10021 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
    10081 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
    10141 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
    10201 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
    10261 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
    10321 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
    10381 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattctg
    10441 cagatatccg gactagtgat ctaattctac cgggtagggg aggcgctttt cccaaggcag
    10501 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
    10561 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
    10621 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
    10681 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
    10741 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
    10801 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
    10861 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
    10921 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
    10981 caatatggga tcggccattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
    11041 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
    11101 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
    11161 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
    11221 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
    11281 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
    11341 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
    11401 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
    11461 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
    11521 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
    11581 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
    11641 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
    11701 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
    11761 ctatcgcctt cttgacgagt tcttctgagg ggatccgtcg agggcccggt acctttaaga
    11821 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
    11881 gaagggctag ctcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
    11941 tggttagacc agatctgagc ctgggagctc tctggctgcc tagggaaccc actgcttaag
    12001 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
    12061 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
    12121 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
    12181 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    12241 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    12301 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc
    12361 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
    12421 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc gtcgagacgt
    12481 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
    12541 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
    12601 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
    12661 cctgaatggc gaatggcgcg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
    12721 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
    12781 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
    12841 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
    12901 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
    12961 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
    13021 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
    13081 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caatttcc
//
