LOCUS       Exported                3452 bp ds-DNA     circular SYN 15-MAY-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Yiping Qi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Feb 14, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    25..48
                     /label=M13-F1
                     /note="M13-F1"
     primer_bind     41..58
                     /label=M13-fwd
     misc_feature    42..57
                     /label=M13-F
     misc_feature    60..172
                     /label=attL5
     promoter        206..582
                     /label=OsU3 promoter
                     /note="OsU3 promoter"
     primer_bind     206..228
                     /label=Set 1 - Pair 1
     misc_structure  584..615
                     /label=guide RNA cloning site
                     /note="guide RNA cloning site"
     misc_RNA        616..748
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    700..717
                     /label=Loop1
     misc_feature    707
                     /label=Insertion
     misc_feature    724..739
                     /label=Loop2
     terminator      755..828
                     /label=OsU6-2 terminator
                     /note="OsU6-2 terminator"
     misc_feature    836..935
                     /label=attL2
     primer_bind     complement(952..973)
                     /label=T7
     primer_bind     complement(972..992)
                     /label=M13-rev
     misc_feature    1070..1203
                     /label=Spn promoter
     misc_feature    1204..2214
                     /label=SpnR
     misc_feature    2281..2954
                     /label=pUC origin
ORIGIN
        1 cgcgttaacg cttgcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct
       61 taagctcggg cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
      121 attgatgagc aatgcttttt tataatgcca actttgtata caaaagttgc cccatggcgt
      181 tccctctaga taacgcagga tcctaaaggg atctttaaac atacgaacag atcacttaaa
      241 gttcttctga agcaacttaa agttatcagg catgcatgga tcttggagga atcagatgtg
      301 cagtcaggga ccatagcaca ggacaggcgt cttctactgg tgctaccagc aaatgctgga
      361 agccgggaac actgggtacg ttggaaacca cgtgatgtgg agtaagataa actgtaggag
      421 aaaagcattt cgtagtgggc catgaagcct ttcaggacat gtattgcagt atgggccggc
      481 ccattacgca attggacgac aacaaagact agtattagta ccacctcggc tatccacata
      541 gatcaaagct ggtttaaaag agttgtgcag atgatccgtg gcagagacga gatctagtct
      601 gagtcgaccg tctctgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
      661 tcaacttgaa aaagtggcac cgagtcggtg cgggagcaca tgaggaatca cccatgtgcg
      721 actcccacag tcactgggga gtcttccctt ttttgtccct tcgaagggca attctgcaga
      781 tatccatcac actggcggcc gctcgaggtc gagggtatcg ataagcttga attcgaccca
      841 gctttcttgt acaaagttgg cattataaaa aataattgct catcaatttg ttgcaacgaa
      901 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
      961 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     1021 tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctggac cagccaggac
     1081 agaaatgcct cgacttcgct gctgcccaag gttgccgggt gacgcacacc gtggaaacgg
     1141 atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     1201 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     1261 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     1321 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     1381 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc
     1441 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     1501 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     1561 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     1621 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     1681 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     1741 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     1801 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     1861 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     1921 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     1981 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     2041 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     2101 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     2161 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg
     2221 agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     2281 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2341 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2401 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     2461 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2521 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2581 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2641 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat
     2701 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2761 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2821 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2881 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     2941 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3001 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3061 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3121 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     3181 attaatacgc gtaccgctcg ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     3241 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     3301 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     3361 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     3421 gttttatttg atgcctggca gttccctact ct
//
