LOCUS       Exported                4796 bp ds-DNA     circular SYN 16-FEB-2021
DEFINITION  4 fragments assembled using overlaps.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 16, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(268..295)
                     /label=rrnB T2
                     /note="transcription termination sequence"
     terminator      complement(427..470)
                     /label=rrnB T1
                     /note="transcription termination sequence"
     primer_bind     537..552
                     /label=M13 forward
     misc_feature    563..1232
                     /label=HAL
     misc_feature    1119..1232
                     /label=exon 2'
     misc_feature    1172..1174
                     /label=Start
     misc_feature    1320..1628
                     /label=SV40 ori
     promoter        1675..1730
                     /label=EM-7
     misc_feature    1749..2147
                     /label=Blasticidin
     misc_feature    2305..2433
                     /label=SV40 polyA'
     misc_feature    2454..2599
                     /label='exon 2
     misc_feature    2461..2960
                     /label=HAR e2
     promoter        complement(3034..3053)
                     /label=T7
                     /note="T7 promoter"
     primer_bind     3061..3077
                     /label=M13 reverse
     gene            3190..3999
                     /label=Kanamycin
                     /note="Kanamycin resistance gene"
     rep_origin      4120..4793
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcgcggcc gcgagcaggt gaaaccggac tacagctttt ctccacggac
      601 tctgtttgcc atgtttattt ctttattctt gattacttca cactttacgc attttccctt
      661 cttgtaactg tagattttgc ctcctattag ttatgaaagt ttaaaacccg ggggagagtg
      721 ggcttacaaa ttttcaatgt atttttgtat agtcttttga gattagagtg aagttctaaa
      781 aagtaagaga accttttgtg aaaatatttt aattgacact actaagagtt taaaatagta
      841 ttggtggtga aaaatcgtta aaagtatctg attttacttg caaaattact gtattttccc
      901 acaagaggag tccttacaac attcttgttt ttagaagggt ttgtttccat cattcattta
      961 aattcataaa gataacgttt tcatgggtgg agaagtctat tgggaaagtc ataattcgaa
     1021 tttctatctt gctttgcagt ttgctttcta tgataagttg aaattattac ttgatgttca
     1081 aggtagtctc tacatcatcc tttcaatatt tctgctaggt tcgatacaag aggctgtttt
     1141 cctagcgtgg cttgctgcct ttggtaagaa catgtcgtcc atcttgccat tcacgccgcc
     1201 agttgtgaag agactgctgg gatggaagaa gtctgccgat ttcggcctat tggttaaaaa
     1261 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     1321 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     1381 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     1441 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     1501 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     1561 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     1621 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     1681 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     1741 aactaaacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg
     1801 gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct
     1861 agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca
     1921 gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc
     1981 gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt
     2041 ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca
     2101 gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaagc acttcgtggc
     2161 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     2221 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     2281 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     2341 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     2401 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctaggagagc
     2461 agaatgggca ggaagaaaag tggtgtgaga aagcagtgaa aagtctggtg aagaagctaa
     2521 agaaaacagg acgattagat gagcttgaga aagccatcac cactcaaaac tgtaatacta
     2581 aatgtgttac cataccaagg taagttttgt tagatcccag gtttgatcaa attatgtcaa
     2641 ggaatctgaa ggaaagttac tgaatttgtg ttcctttcaa gttgcctgta aaaagtgatg
     2701 attgaaatat gactgttttt aaccttgtat aaattgtttt tgctagctga cttgttttat
     2761 aaattatttt cttgaatagt gaggtttaat caagctaaat aagatactta gatttattat
     2821 cttcattgcc tacatttgta tgttatcctc atttccgtga ttgaatgata cttgaataaa
     2881 gaattgaagc tttttctatc actaacatag tagcaaccta cagttttctc ctcctttagt
     2941 caaaactttc atcaggggtg aaaagaggct gctcactttt taatgaattt tatatggttt
     3001 ttctttgcgt tgcagcattg cttccgcggc cgcatcccct atagtgagtc gtattacatg
     3061 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     3121 caagataaaa taatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     3181 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     3241 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     3301 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     3361 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     3421 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     3481 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     3541 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     3601 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     3661 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     3721 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     3781 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     3841 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     3901 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     3961 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     4021 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     4081 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4141 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4201 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4261 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     4321 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4381 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4441 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4501 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     4561 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4621 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4681 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4741 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgtt
//
