LOCUS       Exported               13396 bp ds-DNA     circular SYN 26-OCT-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13396)
  AUTHORS   Kyle
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 15, 2021 from SnapGene Viewer 5.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13396
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(389..1249)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1250..1354)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    1402..1536
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             1704..2337
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2384..2509
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3006..3239
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3766..3883
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3948..4322)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(4332..4350)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4342..4389)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     misc_feature    5345..6262
                     /label=sequenced
     CDS             5586..5651
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5657
                     /label=C to G
     gene            5658..7817
                     /label=PAK7 V593I
     primer_bind     6226..6251
                     /label=PAK5 F2-F
     primer_bind     complement(6296..6322)
                     /label=PAK5 F1-R
     primer_bind     6922..6943
                     /label=PAK5 F3-F
     misc_feature    6968..7896
                     /label=sequenced
     misc_feature    7434..7436
                     /label=V593I
     primer_bind     7529..7552
                     /label=PAK5 F4-F
     misc_feature    7572..8526
                     /label=sequenced
     CDS             8452..9054
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9070..9658
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
     CDS             complement(9541..9552)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9865..10098
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10205..10429
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10475..10903
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        10917..11246
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11097..11232
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    12454..12575
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12624..12640)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12648..12664
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12672..12702)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12717..12738
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(join(13026..13396,1..218))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
       61 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      121 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      181 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
      241 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
      301 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
      361 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
      421 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
      481 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
      541 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
      601 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
      661 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
      721 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
      781 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
      841 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
      901 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
      961 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     1021 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     1081 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     1141 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     1201 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     1261 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     1321 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     1381 acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc
     1441 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1501 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     1561 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     1621 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     1681 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
     1741 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     1801 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     1861 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     1921 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     1981 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     2041 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     2101 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     2161 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     2221 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     2281 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     2341 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     2401 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     2461 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     2521 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     2581 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     2641 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     2701 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     2761 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     2821 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     2881 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     2941 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     3001 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     3061 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     3121 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     3181 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     3241 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     3301 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     3361 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     3421 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     3481 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     3541 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     3601 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     3661 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     3721 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     3781 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     3841 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     3901 cagcagagat ccagtttggt tagtaccggg cccgctctag tccggaatca gtcctgctcc
     3961 tcggccacga agtgcacgca gttgccggcc gggtcgcgca gggcgaactc ccgcccccac
     4021 ggctgctcgc cgatctcggt catggccggc ccggaggcgt cccggaagtt cgtggacacg
     4081 acctccgacc actcggcgta cagctcgtcc aggccgcgca cccacaccca ggccagggtg
     4141 ttgtccggca ccacctggtc ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc
     4201 acaccggcga agtcgtcctc cacgaagtcc cgggagaacc cgagccggtc ggtccagaac
     4261 tcgaccgctc cggcgacgtc gcgcgcggtg agcaccggaa cggcactggt caacttggcc
     4321 atggtggccc tcctatagtg agtcgtatta tactatgccg atatactatg ccgatgatta
     4381 attgtcaaca cgtgctgcag gtccgaggtt ctaggctgcc acgtcagacg aagggcgcag
     4441 cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc
     4501 ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag
     4561 ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga
     4621 ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg
     4681 tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg
     4741 ctgtgatcgt cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc
     4801 gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg
     4861 cgagcaaggt tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc
     4921 gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg
     4981 gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta
     5041 ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact
     5101 ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt
     5161 gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg
     5221 cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca
     5281 ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt
     5341 gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta
     5401 agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag
     5461 ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga
     5521 ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac gctagcggat
     5581 ccatggacta caaagaccat gacggtgatt ataaagatca tgatatcgat tacaaggatg
     5641 acgatgacaa gctagccatg tttgggaaga aaaagaaaaa gattgaaata tctggcccgt
     5701 ccaactttga acacagggtt catactgggt ttgatccaca agagcagaag tttaccggcc
     5761 ttccccagca gtggcacagc ctgttagcag atacggccaa caggccaaag cctatggtgg
     5821 acccttcatg catcacaccc atccagctgg ctcctatgaa gacaatcgtt agaggaaaca
     5881 aaccctgcaa ggaaacctcc atcaacggcc tgctagagga ttttgacaac atctcggtga
     5941 ctcgctccaa ctccctaagg aaagaaagcc cacccacccc agatcaggga gcctccagcc
     6001 acggtccagg ccacgcggaa gaaaatggct tcatcacctt ctcccagtat tccagcgaat
     6061 ccgatactac tgctgactac acgaccgaaa agtacaggga gaagagtctc tatggagatg
     6121 atctggatcc gtattataga ggcagccacg cagccaagca aaatgggcac gtaatgaaaa
     6181 tgaagcacgg ggaggcctac tattctgagg tgaagccttt gaaatccgat tttgccagat
     6241 tttctgccga ttatcactca catttggact cactgagcaa accaagtgaa tacagtgacc
     6301 tcaagtggga gtatcagaga gcctcgagta gctcccctct ggattattca ttccaattca
     6361 caccttctag aactgcaggg accagcgggt gctccaagga gagcctggcg tacagtgaaa
     6421 gtgaatgggg acccagcctg gatgactatg acaggaggcc aaagtcttcg tacctgaatc
     6481 agacaagccc tcagcccacc atgcggcaga ggtccaggtc aggctcggga ctccaggaac
     6541 cgatgatgcc atttggagca agtgcattta aaacccatcc ccaaggacac tcctacaact
     6601 cctacaccta ccctcgcttg tccgagccca caatgtgcat tccaaaggtg gattacgatc
     6661 gagcacagat ggtcctcagc cctccactgt cagggtctga cacctacccc aggggccctg
     6721 ccaaactacc tcaaagtcaa agcaaatcgg gctattcctc aagcagtcac cagtacccgt
     6781 ctgggtacca caaagccacc ttgtaccatc acccctccct gcagagcagt tcgcagtaca
     6841 tctccacggc ttcctacctg agctccctca gcctctcatc cagcacctac ccgccgccca
     6901 gctggggctc ctcctccgac cagcagccct ccagggtgtc ccatgaacag tttcgggcgg
     6961 ccctgcagct ggtggtcagc ccaggagacc ccagggaata cttggccaac tttatcaaaa
     7021 tcggggaagg ctcaaccggc atcgtatgca tcgccaccga gaaacacaca gggaaacaag
     7081 ttgcagtgaa gaaaatggac ctccggaagc aacagagacg agaactgctt ttcaatgagg
     7141 tcgtgatcat gcgggattac caccatgaca atgtggttga catgtacagc agctaccttg
     7201 tcggcgatga gctctgggtg gtcatggagt ttctagaagg tggtgccttg acagacattg
     7261 tgactcacac cagaatgaat gaagaacaga tagctactgt ctgcctgtca gttctgagag
     7321 ctctctccta ccttcataac caaggagtga ttcacaggga cataaaaagt gactccatcc
     7381 tcctgacaag cgatggccgg ataaagttgt ctgattttgg tttctgtgct caaatttcca
     7441 aagaggtgcc gaagaggaaa tcattggttg gcactcccta ctggatggcc cctgaggtga
     7501 tttctaggct accttatggg acagaggtgg acatctggtc cctcgggatc atggtgatag
     7561 aaatgattga tggcgagccc ccctacttca atgagcctcc cctccaggcg atgcggagga
     7621 tccgggacag tttacctcca agagtgaagg acctacacaa ggtttcttca gtgctccggg
     7681 gattcctaga cttgatgttg gtgagggagc cctctcagag agcaacagcc caggaactcc
     7741 tcggacatcc attcttaaaa ctagcaggtc caccgtcttg cattgtcccc ctcatgagac
     7801 aatacaggca tcactgaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga
     7861 gttaacgcaa attccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     7921 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     7981 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     8041 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     8101 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     8161 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     8221 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     8281 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     8341 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     8401 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgataatac catggccacc
     8461 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc
     8521 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac
     8581 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     8641 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     8701 gaagcggggg cggtgttcgc cgagatcggc tcgcgcatgg ccgagttgag cggttcccgg
     8761 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     8821 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     8881 gtcgtgctcc ccggagtgga ggcggccgag cgcgctgggg tgcccgcctt cctggagacc
     8941 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     9001 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagttcgc
     9061 gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     9121 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     9181 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     9241 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     9301 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     9361 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     9421 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     9481 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     9541 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     9601 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     9661 ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     9721 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     9781 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     9841 actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagatc
     9901 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     9961 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
    10021 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
    10081 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
    10141 caaagaaatg aatatcagag agtgagaggc cttgacattg tttaaacccg ctgatcagcc
    10201 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
    10261 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
    10321 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
    10381 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
    10441 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
    10501 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
    10561 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
    10621 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
    10681 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
    10741 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
    10801 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
    10861 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
    10921 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
    10981 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
    11041 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
    11101 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
    11161 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
    11221 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
    11281 ctgatcagca cgtgatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
    11341 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
    11401 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
    11461 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
    11521 aagtgcttga cattggggaa ttaattcagc gagagcctga cctattgcat ctcccgccgt
    11581 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
    11641 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
    11701 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
    11761 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
    11821 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
    11881 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
    11941 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
    12001 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
    12061 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
    12121 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
    12181 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
    12241 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
    12301 actcgtccga gggcaaagga atagcacgtg ctacgagatt tcgattccac cgccgccttc
    12361 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
    12421 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
    12481 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    12541 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
    12601 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    12661 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    12721 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    12781 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    12841 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    12901 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    12961 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    13021 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    13081 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    13141 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    13201 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    13261 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    13321 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    13381 actggtaaca ggatta
//
