LOCUS       Exported               13396 bp ds-DNA     circular SYN 09-MAR-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 15, 2021 from SnapGene Viewer 5.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13396
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     misc_feature    3633..4558
                     /label=sequenced
     primer_bind     3821..3848
                     /label=trip Z rtTA3 seq-F
     primer_bind     3859..3898
                     /label=pLenti-PAK5 A-F
     CDS             3883..3948
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    3955..6114
                     /label=PAK7 E144K
     primer_bind     4374..4398
                     /label=PAK5 E144K B-F
     primer_bind     complement(4374..4398)
                     /label=PAK5 E144K A-R
     misc_feature    4384..4386
                     /label=E144K
     primer_bind     4523..4548
                     /label=PAK5 F2 F
     primer_bind     complement(4593..4619)
                     /label=PAK5 F1 R
     primer_bind     5219..5240
                     /label=PAK5 F3 F
     primer_bind     complement(5269..5290)
                     /label=PAK5 F2 R
     primer_bind     5826..5849
                     /label=PAK5 F4 F
     misc_feature    5864..6798
                     /label=sequenced
     misc_feature    5889..5902
                     /label=Feature 30
     primer_bind     complement(5919..5942)
                     /label=PAK5 F3 R
     primer_bind     complement(6097..6133)
                     /label=pLenti-PAK5 B-R
     CDS             6749..7351
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    7367..7955
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
     CDS             complement(7838..7849)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8162..8395
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8502..8726
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8772..9200
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9214..9543
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9394..9529
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    10751..10872
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10921..10937)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10945..10961
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10969..10999)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11014..11035
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(11323..11911)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12082..12942)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12943..13047)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13095..13229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta ggctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc
     2761 ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc
     2821 agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga
     2881 gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg
     2941 gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc
     3001 agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta
     3061 gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc
     3121 gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg
     3181 gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg
     3241 tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca
     3301 aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca
     3361 ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt
     3421 tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
     3481 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac
     3541 ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg
     3601 taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt
     3661 cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga
     3721 actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg
     3781 taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc
     3841 tggccgtttt tggctttttt gttagacgct agcggatcca tggactacaa agaccatgac
     3901 ggtgattata aagatcatga tatcgattac aaggatgacg atgacaagct agcnatgttt
     3961 gggaagaaaa agaaaaagat tgaaatatct ggcccgtcca actttgaaca cagggttcat
     4021 actgggtttg atccacaaga gcagaagttt accggccttc cccagcagtg gcacagcctg
     4081 ttagcagata cggccaacag gccaaagcct atggtggacc cttcatgcat cacacccatc
     4141 cagctggctc ctatgaagac aatcgttaga ggaaacaaac cctgcaagga aacctccatc
     4201 aacggcctgc tagaggattt tgacaacatc tcggtgactc gctccaactc cctaaggaaa
     4261 gaaagcccac ccaccccaga tcagggagcc tccagccacg gtccaggcca cgcggaagaa
     4321 aatggcttca tcaccttctc ccagtattcc agcgaatccg atactactgc tgactacacg
     4381 accaaaaagt acagggagaa gagtctctat ggagatgatc tggatccgta ttatagaggc
     4441 agccacgcag ccaagcaaaa tgggcacgta atgaaaatga agcacgggga ggcctactat
     4501 tctgaggtga agcctttgaa atccgatttt gccagatttt ctgccgatta tcactcacat
     4561 ttggactcac tgagcaaacc aagtgaatac agtgacctca agtgggagta tcagagagcc
     4621 tcgagtagct cccctctgga ttattcattc caattcacac cttctagaac tgcagggacc
     4681 agcgggtgct ccaaggagag cctggcgtac agtgaaagtg aatggggacc cagcctggat
     4741 gactatgaca ggaggccaaa gtcttcgtac ctgaatcaga caagccctca gcccaccatg
     4801 cggcagaggt ccaggtcagg ctcgggactc caggaaccga tgatgccatt tggagcaagt
     4861 gcatttaaaa cccatcccca aggacactcc tacaactcct acacctaccc tcgcttgtcc
     4921 gagcccacaa tgtgcattcc aaaggtggat tacgatcgag cacagatggt cctcagccct
     4981 ccactgtcag ggtctgacac ctaccccagg ggccctgcca aactacctca aagtcaaagc
     5041 aaatcgggct attcctcaag cagtcaccag tacccgtctg ggtaccacaa agccaccttg
     5101 taccatcacc cctccctgca gagcagttcg cagtacatct ccacggcttc ctacctgagc
     5161 tccctcagcc tctcatccag cacctacccg ccgcccagct ggggctcctc ctccgaccag
     5221 cagccctcca gggtgtccca tgaacagttt cgggcggccc tgcagctggt ggtcagccca
     5281 ggagacccca gggaatactt ggccaacttt atcaaaatcg gggaaggctc aaccggcatc
     5341 gtatgcatcg ccaccgagaa acacacaggg aaacaagttg cagtgaagaa aatggacctc
     5401 cggaagcaac agagacgaga actgcttttc aatgaggtcg tgatcatgcg ggattaccac
     5461 catgacaatg tggttgacat gtacagcagc taccttgtcg gcgatgagct ctgggtggtc
     5521 atggagtttc tagaaggtgg tgccttgaca gacattgtga ctcacaccag aatgaatgaa
     5581 gaacagatag ctactgtctg cctgtcagtt ctgagagctc tctcctacct tcataaccaa
     5641 ggagtgattc acagggacat aaaaagtgac tccatcctcc tgacaagcga tggccggata
     5701 aagttgtctg attttggttt ctgtgctcaa gtttccaaag aggtgccgaa gaggaaatca
     5761 ttggttggca ctccctactg gatggcccct gaggtgattt ctaggctacc ttatgggaca
     5821 gaggtggaca tctggtccct cgggatcatg gtgatagaaa tgattgatgg cgagcccccc
     5881 tacttcaatg agcctcccct ccaggcgatg cggaggatcc gggacagttt acctccaaga
     5941 gtgaaggacc tacacaaggt ttcttcagtg ctccggggat tcctagactt gatgttggtg
     6001 agggagccct ctcagagagc aacagcccag gaactcctcg gacatccatt cttaaaacta
     6061 gcaggtccac cgtcttgcat tgtccccctc atgagacaat acaggcatca ctgaattctg
     6121 cagatatcca gcacagtggc ggccgctcga gtctagagtt aacgcaaatt ccgcccctct
     6181 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     6241 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     6301 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     6361 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     6421 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     6481 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     6541 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     6601 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     6661 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     6721 gttttccttt gaaaaacacg ataataccat ggccaccgag tacaagccca cggtgcgcct
     6781 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta
     6841 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca
     6901 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     6961 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     7021 gatcggctcg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     7081 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     7141 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     7201 ggccgagcgc gctggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     7261 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     7321 ctggtgcatg acccgcaagc ccggtgcctg agttcgcgtc tggaacaatc aacctctgga
     7381 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7441 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7501 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7561 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7621 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7681 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7741 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     7801 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7861 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7921 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac
     7981 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     8041 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     8101 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga
     8161 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     8221 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8281 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8341 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     8401 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     8461 gagaggcctt gacattgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     8521 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     8581 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     8641 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     8701 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     8761 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     8821 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     8881 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     8941 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     9001 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     9061 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     9121 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     9181 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     9241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     9301 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9361 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9421 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9481 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9541 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gatgaaaaag
     9601 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     9661 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     9721 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     9781 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaatta
     9841 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     9901 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
     9961 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
    10021 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
    10081 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
    10141 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
    10201 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
    10261 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
    10321 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
    10381 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
    10441 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
    10501 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
    10561 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata
    10621 gcacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
    10681 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
    10741 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    10801 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    10861 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
    10921 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    10981 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    11041 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    11101 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    11161 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    11221 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    11281 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    11341 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    11401 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    11461 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    11521 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    11581 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    11641 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    11701 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    11761 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    11821 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    11881 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    11941 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    12001 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    12061 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    12121 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    12181 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    12241 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    12301 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    12361 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    12421 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    12481 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    12541 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    12601 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    12661 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    12721 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    12781 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    12841 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    12901 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    12961 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    13021 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
    13081 cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    13141 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    13201 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
    13261 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
    13321 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
    13381 ctacaaactt agtagt
//
