LOCUS       pJAK112                 9146 bp    DNA     circular SYN 17-FEB-2021
DEFINITION  pMTLSC7215 slpA KO (SacI BamHI varient).
ACCESSION   JQ040260
VERSION     JQ040260.1  GI:390517381
KEYWORDS    .
SOURCE      Cloning vector pMTL-SC7215
  ORGANISM  Cloning vector pMTL-SC7215
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Cartman,S.T., Kelly,M.L., Heeg,D., Heap,J.T. and Minton,N.P.
  TITLE     Precise Manipulation of the Clostridium difficile Chromosome
            Reveals a Lack of Association between the tcdC Genotype and Toxin
            Production
  JOURNAL   Appl. Environ. Microbiol. 78 (13), 4683-4690 (2012)
  PUBMED    22522680
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Cartman,S.T., Kelly,M.L., Heap,J.T. and Minton,N.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-2011) Centre for Biomolecular Sciences,
            University of Nottingham, University Park, Nottingham NG7 2RD,
            United Kingdom
FEATURES             Location/Qualifiers
     terminator      9..62
                     /note="derived from CD0164 gene of Clostridium difficile
                     630"
     CDS             318..1601
                     /gene="codA"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="AFL93715.1"
                     /db_xref="GI:390517382"
                     /translation="MSNNALQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHHEGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     misc_feature    1641^1642
                     /Original_Bases="GAGCTCGGTACCCGGGGATCCT"
                     /label="GAGCTCGGTACCCGGGGATCCT"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    1672..1677
                     /Recognition_pattern="C^TCGAG"
                     /annotation_group="CTCGAG"
                     /label="XhoI"
                     /note="Geneious type: restriction site"
     terminator      1865..1906
                     /note="derived from fdx gene of Clostridium pasteurianum"
     CDS             2427..3482
                     /gene="repA"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="AFL93716.1"
                     /db_xref="GI:390517383"
                     /translation="MTNYNQKNENKQEVKTALEKCTDKKRKNPRFTSYIAPLTTKKNI
                     ERTSTCGDYLFMLSDADLEHFKLHKGNFCGNRFCPMCSWRLACKDSLEISILMEHLRK
                     EENKEFIFLTLTTPNVKSYDLNYSIKQYNKSFKKLMERKEVKDITKGYIRKLEVTYQK
                     EKYITKDLWKIKKDYYQKKGLEIGDLEPNFDTYNPHFHVVIAVNKSYFTDKNYYINRE
                     RWLELWKFATKDDSITQVDVRKAKINDYKEVYELAKYSAKDTDYLISRPVFEIFYKAL
                     KGKQVLVFSGFFKDAHKLYKQGKLDVYKKKDEIKYVYIVYYNWCKKQYEKTRIRELTE
                     DEKEELNQDLIDEIEID"
     CDS             complement(3573..4076)
                     /note="orf2"
                     /codon_start=1
                     /transl_table=11
                     /product="putative plasmid replication protein"
                     /protein_id="AFL93717.1"
                     /db_xref="GI:390517384"
                     /translation="MKFKKIILYIFIVIIPLSITGCSINQITTPVPKNYLDNIVKKYV
                     GPEGVQPSFGGKTFYDYKIIDSEKSKNIIKIYLYYIGQEYYIEDDKLVEGTGSASFAT
                     VTIKKENNNYKLVSYEVPKSPAQEDTVKIFPKSVRKKAIHANTPSLKNIEKEAETYFK
                     KELKTKN"
     CDS             4400..5023
                     /gene="catP"
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="AFL93718.1"
                     /db_xref="GI:390517385"
                     /translation="MVFEKIDKNSWNRKEYFDHYFASVPCTYSMTVKVDITQIKEKGM
                     KLYPAMLYYIAMIVNRHSEFRTAINQDGELGIYDEMIPSYTIFHNDTETFSSLWTECK
                     SDFKSFLADYESDTQRYGNNHRMEGKPNAPENIFNVSMIPWSTFDGFNLNLQKGYDYL
                     IPIFTMGKYYKEDNKIILPLAIQVHHAVCDGFHICRFVNELQELINS"
     misc_feature    5100..7499
                     /Original_Bases="AATATATCTTGCCCAAATGTCAAAGCTGGTGGAATGGCTTT
                     TGGTATAAAAAATGAAGTGGCAAGAGAAGTTGTTCGTGAGGTAAGAAACATAACGAAA
                     CTGCCTTTAGTAATAAAATTATCACCCAATGCAGAAGATATTGTAGGAATGGCAAAAG
                     TTTGTGAAGAAGAAGGTGCTGATGGAGTTTCATTAGTTAATACTTTTAAAGCAATGGC
                     AATTGATATAAAAAATAGAAGACCAGTATTTGAAAATGTATATGCTGGATTATCTGGA
                     CCAGCTATAAAACCTATAGCTTTGAGAATGGTACATGAAGTGTGTAAAAATGTAAATA
                     TACCTGTAATGGGAATGGGCGGAATAACTAAAGCCACAGATGCTATAGAGTTTATAAT
                     GGCAGGAGCTACTTGTATCCAAGTTGGAACTGCAAATTTTATGAATCCTAGAATAGGT
                     ATTGAAATAATAGATGGGATTAATGAATTTATGGATAGAGAAGGAATAAAAAGTTTAG
                     ACGAAATAAGAGGAATTATATAAGGTAGAATAAATAGTATTTTAAAGATAAAAAATAT
                     AGATTAAATAAACAATAATTTTATAACATTAACATGGAGGAAAAATAATATGAGTAAA
                     GTAGATGTAGTGGATATATTAAAGAAAAGTGATGCATTGTTGGAGGGACATTTTTTAT
                     TATCTTCAGGAAAACACAGTAATAGATACGTACAATGTGCAAAAGTATTAAGATTTCC
                     AGAATATGCTGCTGAGGTATTAAGTACAGTTGTTGAACAAATAAAAGACTTAGATATA
                     GACTTAGTAGTAGGACCAGCTATGGGTGGAGTAATAGTTTCTTATGAGTTAGGAAGAC
                     AATTAGGAAAAGAAGCTGTATTTACTGAGAGAAAAGACAATACAATGGAGTTAAGAAG
                     AGGATTTGAAGTTAAAAAAGGAGCAAAGATAATAATTGCTGAAGACGTTGTAACTACT
                     GGTAAATCAACTATGGAGACAAAAAGAGTATTAGAAGCCTTAGGTGGAGAAGTTGTAG
                     GTGTTGCATGTATAGCAGATAGAACTAATCATGATATAGGTATGCCTATATATAGTGC
                     TATAAAACTTGATATTCAAGTTTATGAATCTGATGAGTGTCCTTTATGTAAGGAAGGA
                     AAATTACCAGTTGTTAAGCCTGGAAGTAGAGAGTTCAAGGAATTAGGGATGTAATAAC
                     GAATTCTGCATCAAGCTAGCATAAAAATAAGAAGCCTGCATTTGCAGGCTTCTTATTT
                     TTATGGTACCTTAAGACCCACTTTCACATTTAAGTTGTTTTTCTAATCCGCATATGAT
                     CAATTCAAGGCCGAATAAGAAGGCTGGCTCTGCACCTTGGTGATCAAATAATTCGATA
                     GCTTGTCGTAATAATGGCGGCATACTATCAGTAGTAGGTGTTTCCCTTTCTTCTTTAG
                     CGACTTGATGCTCTTGATCTTCCAATACGCAACCTAAAGTAAAATGCCCCACAGCGCT
                     GAGTGCATATAATGCATTCTCTAGTGAAAAACCTTGTTGGCATAAAAAGGCTAATTGA
                     TTTTCGAGAGTTTCATACTGTTTTTCTGTAGGCCGTGTACCTAAATGTACTTTTGCTC
                     CATCGCGATGACTTAGTAAAGCACATCTAAAACTTTTAGCGTTATTACGTAAAAAATC
                     TTGCCAGCTTTCCCCTTCTAAAGGGCAAAAGTGAGTATGGTGCCTATCTAACATCTCA
                     ATGGCTAAGGCGTCGAGCAAAGCCCGCTTATTTTTTACATGCCAATACAATGTAGGCT
                     GCTCTACACCTAGCTTCTGGGCGAGTTTACGGGTTGTTAAACCTTCGATTCCGACCTC
                     ATTAAGCAGCTCTAATGCGCTGTTAATCACTTTACTTTTATCTAATCTAGACATCATT
                     AATTCCTCCTTTTTGTTGACATTATATCATTGATAGAGTTATTTGTCAAACTAGTTTT
                     TTATTTCGATGCCCTGGACTTCATGAAAAACTAAAAAAAATATTGACACTCTATCATT
                     GATAGAGTATAATTAAAATAAGCTTGATCGTAGCGTTAACAGATCTGAGCTCAGAATG
                     TTTTAAACATGATTCTTAATAGTAGGAAGGAGTAAAATCATGGCTAAGGAAAAAAAGA
                     AAGAAAAAAAAGGTTTTTTTAGTTTTAATAAGATGACAAAGGATATGGGAATAGATTT
                     AGGGACTGCAAATACATTAGTTTACATAAAAGGACAAGGTATTGTTGTTAGAGAACCA
                     TCAGTTGTCGCAATAAGAGATGATAGTAAAGAGGTATTAGCAGTAGGTGAAGAAGCCA
                     AAAAAATGATAGGTAGAACACCAGGAAATATAGTAGCTATAAGACCTATGAAAGATGG
                     AGTAATAGCTGATTTTGACATAACTCAATCAATGATTAGCTATTTTATACAAAAAGCG
                     GCTGATAAAAAAGGTGTTGTAAGTCCAAGAATAGCTATATGTGTTCCTTTTGGAGTTA
                     CAGAAGTAGAAAAGAGAGCTATAGAAGAGGCTGCAAGACAAGCAGGAGCTAAAGATGC
                     ATTCCTTATAGAAGAGCCAATGGCAGCGGCTATAGGGGCTGGATTAAAAGTTGAAGAA
                     CCAGAAGGTAATATGGTAGTTGATATTGGTGGAGGTACTTCAGAAATAGCTGTAATAT
                     CTTTAGGAGGAATCGTTACTGCTAAATCTATAAGAATAGGTGGAGACGAATTTGACGA
                     GTCTATAGTGGCTTATGTAAAAAAAGAATATAATCTTATGATAGGTGAAAGAACTGCT
                     GAAAATGTAAAAATTAACATAGGTTCTACATTTAAAGATGATGAAGAAATAAATATGC
                     AAATAAGAGGTAGAGATTTAATCTCAGGATTACCTAAAACAATAGAAATATGCTCTAC
                     AGAAGTTAGAGAAGCATTAAAAGAACCTGTAAGTTCAATAGTAGATGCAATAAAATCT
                     ACACTAGAAAGAACTCCACCAGAATTAGCCTCAGATATAATGGAAAACGGAATAATGC
                     TAACAGGAGGAGGCGCTTTGCTTAGAGGTCTTGATAAGCTTATTACACAGGAAACTGG
                     TATGTCTGTACAAATAGCAGAAACACCTCTTGATTGTGTTGCACTTGGAACAGGTAAA
                     TCTGTTGAAGACCAAGAAATCTTTGAAAAAGTATTAATGATGAATACTAAGAATTAGG
                     ATCGGATCCTATAAGTTTTAATAAAACTTTAAATAGAAAAAGGCTTCTCTCATGAGAA
                     GTCTTTTTTATTTAAACTTAATTATTTATAGTGTTACTTAAAAAATGTAAATTTTTTA
                     GAGTAAAAATTTGTAATATAACATATAACTTAAAAATAAAAAGCAGTTAGCCTTATAA
                     ATGAAGGTTAGCTGTTTTTTTTGTTAAAAATATATTTACAAAATAAGAGATAGTAGCA
                     GTACAATATAAGTATAAGGTAAAAGTATTATATATTAGGGAGGGGTAAGTATGAAAAA
                     TCAATTAAATGAAAATCATAAAAAGGTTGTATTTTTTGGAGTAGGTGCTGTAGGAGCT
                     ACTTTTGCAGAACAATTTTTTAACTCTAAATATGATTTTAAAATTCTTTGTGATAATG
                     AAAGAAAGAAAAGATATTTAGAAGAAGGATTTATAATAAATGGAAAAAGGTATGATTT
                     TAATTATGTAACTAAAGATGAGTATAAACAAGAGGCTGATTTTATAATTATAGGTCTA
                     AAATATAATAATTTAAAAGAAAATATAAAAGAATTAGATGGATTAGTTGGAAAAAATA
                     CAGTTATAATGTCTCTGCTAAATGGAGTTGATAGCGAAGAGATAATAGGAGAAAGATT
                     TGGAATTGGAAAAATGGTATATTCATATGTTACCAATATAGATGCAAAGAAAATCAAT
                     AATAATATTATACACACTACTAATGGGATAATTGTATTTGGTAACAAAGATAATAGTG
                     AAGATAGAAAAACTAATATAATAACTGAAGTCTTTGATGATGTAAATATAGAATATAT
                     TTTATCAAAAGATATTCAACGAGATATGTGGTGGAAGTACATGGTTAATATTGGTGTA
                     AATCAAACTTCAGCTATACTTGGTGCACCTTATGGAGTTTTTCAGAGTTCTGAGCATT
                     TAAGAGAATTAGCAAAATCTGCAATGAGGGAAGTTGTTGCTATAGCACAAGCAAAAGA
                     CATATCTCTTACAGAAGATGATGTTGAAAATTCATTACATAGAATACTAGAACATTCA
                     AAAGAAGGAAGAACATCAATGCTTCAAGACGTGGAAGCTCATAGACCTACAGAAGTAG
                     ATATGTTTTCTAAGAATATCTGCAAACTTGGAAAAAAATATAGCATACCTACTCCTAT
                     AAATCAGACTTTATTTTATATGATAAAAGTAATTGAAAGTAGATTTTAAAGGGTATTT
                     GTAAGAAATATTGATAATTATTGATTTTAAATTTTATTAATGTTATATACTAT"
                     /label="AATATATCTTGCCCAAATGTCAAAGCTGGTGGAATGGCTTTTGGTATAAA
                     AAATGAAGTGGCAAGAGAAGTTGTTCGTGAGGTAAGAAACATAACGAAACTGCCTTTA
                     GTAATAAAATTATCACCCAATGCAGAAGATATTGTAGGAATGGCAAAAGTTTGTGAAG
                     AAGAAGGTGCTGATGGAGTTTCATTAGTTAATACTTTTAAAGCAATGGCAATTGATAT
                     AAAAAATAGAAGACCAGTATTTGAAAATGTATATGCTGGATTATCTGGACCAGCTATA
                     AAACCTATAGCTTTGAGAATGGTACATGAAGTGTGTAAAAATGTAAATATACCTGTAA
                     TGGGAATGGGCGGAATAACTAAAGCCACAGATGCTATAGAGTTTATAATGGCAGGAGC
                     TACTTGTATCCAAGTTGGAACTGCAAATTTTATGAATCCTAGAATAGGTATTGAAATA
                     ATAGATGGGATTAATGAATTTATGGATAGAGAAGGAATAAAAAGTTTAGACGAAATAA
                     GAGGAATTATATAAGGTAGAATAAATAGTATTTTAAAGATAAAAAATATAGATTAAAT
                     AAACAATAATTTTATAACATTAACATGGAGGAAAAATAATATGAGTAAAGTAGATGTA
                     GTGGATATATTAAAGAAAAGTGATGCATTGTTGGAGGGACATTTTTTATTATCTTCAG
                     GAAAACACAGTAATAGATACGTACAATGTGCAAAAGTATTAAGATTTCCAGAATATGC
                     TGCTGAGGTATTAAGTACAGTTGTTGAACAAATAAAAGACTTAGATATAGACTTAGTA
                     GTAGGACCAGCTATGGGTGGAGTAATAGTTTCTTATGAGTTAGGAAGACAATTAGGAA
                     AAGAAGCTGTATTTACTGAGAGAAAAGACAATACAATGGAGTTAAGAAGAGGATTTGA
                     AGTTAAAAAAGGAGCAAAGATAATAATTGCTGAAGACGTTGTAACTACTGGTAAATCA
                     ACTATGGAGACAAAAAGAGTATTAGAAGCCTTAGGTGGAGAAGTTGTAGGTGTTGCAT
                     GTATAGCAGATAGAACTAATCATGATATAGGTATGCCTATATATAGTGCTATAAAACT
                     TGATATTCAAGTTTATGAATCTGATGAGTGTCCTTTATGTAAGGAAGGAAAATTACCA
                     GTTGTTAAGCCTGGAAGTAGAGAGTTCAAGGAATTAGGGATGTAATAACGAATTCTGC
                     ATCAAGCTAGCATAAAAATAAGAAGCCTGCATTTGCAGGCTTCTTATTTTTATGGTAC
                     CTTAAGACCCACTTTCACATTTAAGTTGTTTTTCTAATCCGCATATGATCAATTCAAG
                     GCCGAATAAGAAGGCTGGCTCTGCACCTTGGTGATCAAATAATTCGATAGCTTGTCGT
                     AATAATGGCGGCATACTATCAGTAGTAGGTGTTTCCCTTTCTTCTTTAGCGACTTGAT
                     GCTCTTGATCTTCCAATACGCAACCTAAAGTAAAATGCCCCACAGCGCTGAGTGCATA
                     TAATGCATTCTCTAGTGAAAAACCTTGTTGGCATAAAAAGGCTAATTGATTTTCGAGA
                     GTTTCATACTGTTTTTCTGTAGGCCGTGTACCTAAATGTACTTTTGCTCCATCGCGAT
                     GACTTAGTAAAGCACATCTAAAACTTTTAGCGTTATTACGTAAAAAATCTTGCCAGCT
                     TTCCCCTTCTAAAGGGCAAAAGTGAGTATGGTGCCTATCTAACATCTCAATGGCTAAG
                     GCGTCGAGCAAAGCCCGCTTATTTTTTACATGCCAATACAATGTAGGCTGCTCTACAC
                     CTAGCTTCTGGGCGAGTTTACGGGTTGTTAAACCTTCGATTCCGACCTCATTAAGCAG
                     CTCTAATGCGCTGTTAATCACTTTACTTTTATCTAATCTAGACATCATTAATTCCTCC
                     TTTTTGTTGACATTATATCATTGATAGAGTTATTTGTCAAACTAGTTTTTTATTTCGA
                     TGCCCTGGACTTCATGAAAAACTAAAAAAAATATTGACACTCTATCATTGATAGAGTA
                     TAATTAAAATAAGCTTGATCGTAGCGTTAACAGATCTGAGCTCAGAATGTTTTAAACA
                     TGATTCTTAATAGTAGGAAGGAGTAAAATCATGGCTAAGGAAAAAAAGAAAGAAAAAA
                     AAGGTTTTTTTAGTTTTAATAAGATGACAAAGGATATGGGAATAGATTTAGGGACTGC
                     AAATACATTAGTTTACATAAAAGGACAAGGTATTGTTGTTAGAGAACCATCAGTTGTC
                     GCAATAAGAGATGATAGTAAAGAGGTATTAGCAGTAGGTGAAGAAGCCAAAAAAATGA
                     TAGGTAGAACACCAGGAAATATAGTAGCTATAAGACCTATGAAAGATGGAGTAATAGC
                     TGATTTTGACATAACTCAATCAATGATTAGCTATTTTATACAAAAAGCGGCTGATAAA
                     AAAGGTGTTGTAAGTCCAAGAATAGCTATATGTGTTCCTTTTGGAGTTACAGAAGTAG
                     AAAAGAGAGCTATAGAAGAGGCTGCAAGACAAGCAGGAGCTAAAGATGCATTCCTTAT
                     AGAAGAGCCAATGGCAGCGGCTATAGGGGCTGGATTAAAAGTTGAAGAACCAGAAGGT
                     AATATGGTAGTTGATATTGGTGGAGGTACTTCAGAAATAGCTGTAATATCTTTAGGAG
                     GAATCGTTACTGCTAAATCTATAAGAATAGGTGGAGACGAATTTGACGAGTCTATAGT
                     GGCTTATGTAAAAAAAGAATATAATCTTATGATAGGTGAAAGAACTGCTGAAAATGTA
                     AAAATTAACATAGGTTCTACATTTAAAGATGATGAAGAAATAAATATGCAAATAAGAG
                     GTAGAGATTTAATCTCAGGATTACCTAAAACAATAGAAATATGCTCTACAGAAGTTAG
                     AGAAGCATTAAAAGAACCTGTAAGTTCAATAGTAGATGCAATAAAATCTACACTAGAA
                     AGAACTCCACCAGAATTAGCCTCAGATATAATGGAAAACGGAATAATGCTAACAGGAG
                     GAGGCGCTTTGCTTAGAGGTCTTGATAAGCTTATTACACAGGAAACTGGTATGTCTGT
                     ACAAATAGCAGAAACACCTCTTGATTGTGTTGCACTTGGAACAGGTAAATCTGTTGAA
                     GACCAAGAAATCTTTGAAAAAGTATTAATGATGAATACTAAGAATTAGGATCGGATCC
                     TATAAGTTTTAATAAAACTTTAAATAGAAAAAGGCTTCTCTCATGAGAAGTCTTTTTT
                     ATTTAAACTTAATTATTTATAGTGTTACTTAAAAAATGTAAATTTTTTAGAGTAAAAA
                     TTTGTAATATAACATATAACTTAAAAATAAAAAGCAGTTAGCCTTATAAATGAAGGTT
                     AGCTGTTTTTTTTGTTAAAAATATATTTACAAAATAAGAGATAGTAGCAGTACAATAT
                     AAGTATAAGGTAAAAGTATTATATATTAGGGAGGGGTAAGTATGAAAAATCAATTAAA
                     TGAAAATCATAAAAAGGTTGTATTTTTTGGAGTAGGTGCTGTAGGAGCTACTTTTGCA
                     GAACAATTTTTTAACTCTAAATATGATTTTAAAATTCTTTGTGATAATGAAAGAAAGA
                     AAAGATATTTAGAAGAAGGATTTATAATAAATGGAAAAAGGTATGATTTTAATTATGT
                     AACTAAAGATGAGTATAAACAAGAGGCTGATTTTATAATTATAGGTCTAAAATATAAT
                     AATTTAAAAGAAAATATAAAAGAATTAGATGGATTAGTTGGAAAAAATACAGTTATAA
                     TGTCTCTGCTAAATGGAGTTGATAGCGAAGAGATAATAGGAGAAAGATTTGGAATTGG
                     AAAAATGGTATATTCATATGTTACCAATATAGATGCAAAGAAAATCAATAATAATATT
                     ATACACACTACTAATGGGATAATTGTATTTGGTAACAAAGATAATAGTGAAGATAGAA
                     AAACTAATATAATAACTGAAGTCTTTGATGATGTAAATATAGAATATATTTTATCAAA
                     AGATATTCAACGAGATATGTGGTGGAAGTACATGGTTAATATTGGTGTAAATCAAACT
                     TCAGCTATACTTGGTGCACCTTATGGAGTTTTTCAGAGTTCTGAGCATTTAAGAGAAT
                     TAGCAAAATCTGCAATGAGGGAAGTTGTTGCTATAGCACAAGCAAAAGACATATCTCT
                     TACAGAAGATGATGTTGAAAATTCATTACATAGAATACTAGAACATTCAAAAGAAGGA
                     AGAACATCAATGCTTCAAGACGTGGAAGCTCATAGACCTACAGAAGTAGATATGTTTT
                     CTAAGAATATCTGCAAACTTGGAAAAAAATATAGCATACCTACTCCTATAAATCAGAC
                     TTTATTTTATATGATAAAAGTAATTGAAAGTAGATTTTAAAGGGTATTTGTAAGAAAT
                     ATTGATAATTATTGATTTTAAATTTTATTAATGTTATATACTAT"
                     /note="Geneious type: Editing History Replacement"
     CDS             <5106..6218
                     /transl_table=11
                     /locus_tag="CDR20291_2683 cwp12"
                     /colour=3
                     /product="surface surface protein"
                     /protein_id="CBE06200.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGSPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNIDTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYSLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVINGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDAREVANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
                     /label="  surface surface protein    CDS      "
     CDS             6530..>7493
                     /transl_table=11
                     /gene="secA2"
                     /locus_tag="CDR20291_2681"
                     /product="preprotein translocase SecA subunit"
                     /protein_id="CBE06196.1"
                     /translation="MSVLDAILDKADEQEIKKLNLIVDKIESLEKDMEIKENEQLKEM
                     TNTFRLRLDKGESLDDILPEAFAVVREVSKRVLGMRQYKVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYEFLGLTVGVILSNQD
                     PSIRKEQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEIDSILIDEAR
                     TPLIIAGDGDEDLKLYELANNFVKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNRPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSKMLKK
                     QGIKHQVLNAKQHDKEAEVISKAGKLDAITIATNMAGRGTDISLGAGDKEEEEKVKNL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIKEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
                     /label="  preprotein translocase SecA subunit  secA2  CDS 
                         "
     CDS             8572..9012
                     /gene="traJ"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid conjugative transfer protein"
                     /protein_id="AFL93719.1"
                     /db_xref="GI:390517386"
                     /translation="MLNGNPALRGWPATAGVTDEGKRMADETKPTRKGSPPIKVYCLP
                     DERRAIEEKAAAAGMSLSAYLLAVGQGYKITGVVDYEHVRELARINGDLGRLGGLLKL
                     WLTDDPRTARFGDATILALLAKIEEKQDELGKVMMGVVRPRAEP"
ORIGIN      
        1 cctgcaggat aaaaaaattg tagataaatt ttataaaata gttttatcta caattttttt
       61 atcaggaaac agctatgacc gcggccgctg tatccatatg accatgatta cgaattcccc
      121 ggatcgagat agtatatgat gcatattctt taaatataga taaagttata gaagcaatag
      181 aagatttagg atttactgta atataaatta cacttttaaa aagtttaaaa acatgataca
      241 ataagttatg gttggaattg ttatccgctc acaattccaa cttatgatta aaattttaag
      301 gaggtgtatt tcataatgtg tcgaataacg ctttacaaac aattattaac gcccggttac
      361 caggcgaaga ggggctgtgg cagattcatc tgcaggacgg aaaaatcagc gccattgatg
      421 cgcaatccgg cgtgatgccc ataactgaaa acagcctgga tgccgaacaa ggtttagtta
      481 taccgccgtt tgtggagcca catattcacc tggacaccac gcaaaccgcc ggacaaccga
      541 actggaatca gtccggcacg ctgtttgaag gcattgaacg ctgggccgag cgcaaagcgt
      601 tattaaccca tgacgatgtg aaacaacgcg catggcaaac gctgaaatgg cagattgcca
      661 acggcattca gcatgtgcgt acccatgtcg atgtttcgga tgcaacgcta actgcgctga
      721 aagcaatgct ggaagtgaag caggaagtcg cgccgtggat tgatctgcaa atcgtcgcct
      781 tccctcagga agggattttg tcgtatccca acggtgaagc gttgctggaa gaggcgttac
      841 gcttaggggc agatgtagtg ggggcgattc cgcattttga atttacccgt gaatacggcg
      901 tggagtcgct gcataaaacc ttcgccctgg cgcaaaaata cgaccgtctc atcgacgttc
      961 actgtgatga gatcgatgac gagcagtcgc gctttgtcga aaccgttgct gccctggcgc
     1021 accatgaagg catgggcgcg cgagtcaccg ccagccacac cacggcaatg cactcctata
     1081 acggggcgta tacctcacgc ctgttccgct tgctgaaaat gtccggtatt aactttgtcg
     1141 ccaacccgct ggtcaatatt catctgcaag gacgtttcga tacgtatcca aaacgtcgcg
     1201 gcatcacgcg cgttaaagag atgctggagt ccggcattaa cgtctgcttt ggtcacgatg
     1261 atgtcttcga tccgtggtat ccgctgggaa cggcgaatat gctgcaagtg ctgcatatgg
     1321 ggctgcatgt ttgccagttg atgggctacg ggcagattaa cgatggcctg aatttaatca
     1381 cccaccacag cgcaaggacg ttgaatttgc aggattacgg cattgccgcc ggaaacagcg
     1441 ccaacctgat tatcctgccg gctgaaaatg ggtttgatgc gctgcgccgt caggttccgg
     1501 tacgttattc ggtacgtggc ggcaaggtga ttgccagcac acaaccggca caaaccaccg
     1561 tatatctgga gcagccagaa gccatcgatt acaaacgttg aacgactggg ttacagcgag
     1621 cttagtttaa agggcgaatt cctagagtcg acgtcacgcg tccatggaga tctcgaggcc
     1681 tgcagacatg caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     1741 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     1801 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     1861 tagcataaaa ataagaagcc tgcatttgca ggcttcttat ttttatggcg cgccgttctg
     1921 aatccttagc taatggttca acaggtaact atgacgaaga tagcaccctg gataagtctg
     1981 taatggattc taaggcattt aatgaagacg tgtatataaa atgtgctaat gaaaaagaaa
     2041 atgcgttaaa agagcctaaa atgagttcaa atggttttga aattgattgg tagtttaatt
     2101 taatatattt tttctattgg ctatctcgat acctatagaa tcttctgttc acttttgttt
     2161 ttgaaatata aaaaggggct ttttagcccc ttttttttaa aactccggag gagtttcttc
     2221 attcttgata ctatacgtaa ctattttcga tttgacttca ttgtcaatta agctagtaaa
     2281 atcaatggtt aaaaaacaaa aaacttgcat ttttctacct agtaatttat aattttaagt
     2341 gtcgagttta aaagtataat ttaccaggaa aggagcaagt tttttaataa ggaaaaattt
     2401 ttccttttaa aattctattt cgttatatga ctaattataa tcaaaaaaat gaaaataaac
     2461 aagaggtaaa aactgcttta gagaaatgta ctgataaaaa aagaaaaaat cctagattta
     2521 cgtcatacat agcaccttta actactaaga aaaatattga aaggacttcc acttgtggag
     2581 attatttgtt tatgttgagt gatgcagact tagaacattt taaattacat aaaggtaatt
     2641 tttgcggtaa tagattttgt ccaatgtgta gttggcgact tgcttgtaag gatagtttag
     2701 aaatatctat tcttatggag catttaagaa aagaagaaaa taaagagttt atatttttaa
     2761 ctcttacaac tccaaatgta aaaagttatg atcttaatta ttctattaaa caatataata
     2821 aatcttttaa aaaattaatg gagcgtaagg aagttaagga tataactaaa ggttatataa
     2881 gaaaattaga agtaacttac caaaaggaaa aatacataac aaaggattta tggaaaataa
     2941 aaaaagatta ttatcaaaaa aaaggacttg aaattggtga tttagaacct aattttgata
     3001 cttataatcc tcattttcat gtagttattg cagttaataa aagttatttt acagataaaa
     3061 attattatat aaatcgagaa agatggttgg aattatggaa gtttgctact aaggatgatt
     3121 ctataactca agttgatgtt agaaaagcaa aaattaatga ttataaagag gtttacgaac
     3181 ttgcgaaata ttcagctaaa gacactgatt atttaatatc gaggccagta tttgaaattt
     3241 tttataaagc attaaaaggc aagcaggtat tagtttttag tggatttttt aaagatgcac
     3301 acaaattgta caagcaagga aaacttgatg tttataaaaa gaaagatgaa attaaatatg
     3361 tctatatagt ttattataat tggtgcaaaa aacaatatga aaaaactaga ataagggaac
     3421 ttacggaaga tgaaaaagaa gaattaaatc aagatttaat agatgaaata gaaatagatt
     3481 aaagtgtaac tatactttat atatatatga ttaaaaaaat aaaaaacaac agcctattag
     3541 gttgttgttt tttattttct ttattaattt ttttaatttt tagtttttag ttctttttta
     3601 aaataagttt cagcctcttt ttcaatattt tttaaagaag gagtatttgc atgaattgcc
     3661 ttttttctaa cagacttagg aaatatttta acagtatctt cttgcgccgg tgattttgga
     3721 acttcataac ttactaattt ataattatta ttttcttttt taattgtaac agttgcaaaa
     3781 gaagctgaac ctgttccttc aactagttta tcatcttcaa tataatattc ttgacctata
     3841 tagtataaat atatttttat tatattttta cttttttctg aatctattat tttataatca
     3901 taaaaagttt taccaccaaa agaaggttgt actccttctg gtccaacata tttttttact
     3961 atattatcta aataattttt gggaactggt gttgtaattt gattaatcga acaaccagtt
     4021 atacttaaag gaattataac tataaaaata tataggatta tctttttaaa tttcattatt
     4081 ggcctccttt ttattaaatt tatgttacca taaaaaggac ataacgggaa tatgtagaat
     4141 atttttaatg tagacaaaat tttacataaa tataaagaaa ggaagtgttt gtttaaattt
     4201 tatagcaaac tatcaaaaat tagggggata aaaatttatg aaaaaaaggt tttcgatgtt
     4261 atttttatgt ttaactttaa tagtttgtgg tttatttaca aattcggccg gccagtgggc
     4321 aagttgaaaa attcacaaaa atgtggtata atatctttgt tcattagagc gataaacttg
     4381 aatttgagag ggaacttaga tggtatttga aaaaattgat aaaaatagtt ggaacagaaa
     4441 agagtatttt gaccactact ttgcaagtgt accttgtacc tacagcatga ccgttaaagt
     4501 ggatatcaca caaataaagg aaaagggaat gaaactatat cctgcaatgc tttattatat
     4561 tgcaatgatt gtaaaccgcc attcagagtt taggacggca atcaatcaag atggtgaatt
     4621 ggggatatat gatgagatga taccaagcta tacaatattt cacaatgata ctgaaacatt
     4681 ttccagcctt tggactgagt gtaagtctga ctttaaatca tttttagcag attatgaaag
     4741 tgatacgcaa cggtatggaa acaatcatag aatggaagga aagccaaatg ctccggaaaa
     4801 catttttaat gtatctatga taccgtggtc aaccttcgat ggctttaatc tgaatttgca
     4861 gaaaggatat gattatttga ttcctatttt tactatgggg aaatattata aagaagataa
     4921 caaaattata cttcctttgg caattcaagt tcatcacgca gtatgtgacg gatttcacat
     4981 ttgccgtttt gtaaacgaat tgcaggaatt gataaatagt taacttcagg tttgtctgta
     5041 actaaaaaca agtatttaag caaaaacatc gtagaaatac ggtgtttttt gttaccctag
     5101 agctctatac aggtgaagca gatgctatga gtgcgtcatc agtagcatca agagatggat
     5161 cacctataat tttaactaat ggtaaaaatg ttccatttga gaaaaaagaa ggtgtgcaat
     5221 gttatgctct tggttcagag gagataataa gtaatgattt agttaaaaaa actaatgcag
     5281 ttagattagc tggagaagat agatttgaga ctaataaaaa agttataaaa catttttata
     5341 gttcggcaaa agaattttat ctttctaaag gttatcaatt agtagatgca gttgctggtt
     5401 cttctatagc aaaaaatgca cctattgtat tagttgacgg aaatagtgat aagtctgtac
     5461 ttagaagtgc tgataagatt acagcactag gaggaataga tgaaaagaca ctagaacaat
     5521 gtttaagtgc atcatcactg gatgcaagtg caccaacaat aactgttgga aatttaaata
     5581 tttatcaagg tgataagttt gatataagta aactgaatat cgtagcaaag gatagcaatg
     5641 gtaacgattt gacaccagag cttataggaa atattgatac tgataaggtg ggtaaataca
     5701 aagttactat aaaagctact gatattggtg gaaagactac atcaataagt gtagaagtaa
     5761 atgtacttga atataagact aatgatatga atagtagtga gtttaaaaga atggtttcta
     5821 gtgaaatgta tagtttggta aattcatata gaaaagaaaa aggtaaagag ccattacaag
     5881 tttctaaaaa cttagaagga ctttctaatt cctggtctaa gtacatggca gataaaggtc
     5941 agttttcaca tgtaataaat ggtaagaaat ctgttgaagt atttactgga tatggcttga
     6001 gaagtgaaga aaatatagct tttgtaccac ttgttacaaa aagtacttat acaacaaagg
     6061 atgctagaga agtagcaaat gtaatattta cagtttggaa aaagtcagat aaatataatg
     6121 aaaatatgtt aaatagtgat tttgtatata ctgggtttgg attatatata ttgcctaatg
     6181 gagaagtgta tgctacacaa gaatttttaa ataagtaatt taaataagta aaatcaaatt
     6241 ttttaagtta ttaaatattg gagtttaagg gtataaaaac tcatatgctt gggtataaat
     6301 ataataatat aaaaaggctt cttatatgag aagtcttttt atattatgtt ttcgaataca
     6361 aaaaaaagag actaaattta gtctcttttt tatgtgagaa atacttaaat atataaagat
     6421 gtaatttatt taagtatgat tattatacta tacaataaaa taattgtcta ctattctata
     6481 attttaaggt ataataataa ttgtatgata aaagaggagg tcaaaaaata tgtcagtttt
     6541 agatgcaatc ttagataaag cggatgaaca agaaattaaa aaattaaatt taatagtaga
     6601 taaaatagaa tctttagaaa aagatatgga aatcaaagag aatgaacaat taaaagaaat
     6661 gacaaatact tttagactta gattagacaa aggtgaaagt ttggatgata tacttccaga
     6721 agcatttgct gttgttagag aggtctcaaa aagagtttta ggaatgcgtc aatataaagt
     6781 tcagttaatt ggaggtatag taattcatca aggaaagata gctgagatga agactggtga
     6841 ggggaaaact ttagttgagg tagctccagt atatctaaat gctttaacag gaaaaggtgt
     6901 acatgtaatt acagtaaatg attatttggc agaacgtgat aaagaactta tgcgtccagt
     6961 atatgaattt ttaggattga ctgtaggtgt aattttatct aaccaagacc caagtataag
     7021 aaaagaacaa tataaatgtg atataactta tggaacaaat agtgaatttg gatttgacta
     7081 tttaagagat aatatggtgc cagatttgtc tcataaagta caaagggaac taaactttgc
     7141 catagtagat gagatagact caatattaat agatgaagct agaactccac ttattattgc
     7201 aggagatggt gatgaagatt taaaacttta tgagttagca aataactttg taaaaactgt
     7261 taaagaagaa gactttgaga tggatagaaa agataaaact atagcattaa cagcaagtgg
     7321 tataagcaaa gctgagtcat tttttggtat aacaaacctt actgatataa agaatataga
     7381 attatatcat catataaatc aagctttaag aggtcataag cttatggaaa aagatgttga
     7441 ctatgttatc tcaaatggag aagtaatgat agttgacgaa tttacaggaa gagggatccc
     7501 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7561 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7621 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7681 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7741 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7801 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7861 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7921 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7981 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8041 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8101 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8161 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8221 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8281 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8341 gtgagcgagg aagcggaaga gcgcccaata cgcagggccc cctgcttcgg ggtcattata
     8401 gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt
     8461 cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc
     8521 ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac
     8581 gggaatcctg ctctgcgagg ctggccggct accgccggcg taacagatga gggcaagcgg
     8641 atggctgatg aaaccaagcc aaccaggaag ggcagcccac ctatcaaggt gtactgcctt
     8701 ccagacgaac gaagagcgat tgaggaaaag gcggcggcgg ccggcatgag cctgtcggcc
     8761 tacctgctgg ccgtcggcca gggctacaaa atcacgggcg tcgtggacta tgagcacgtc
     8821 cgcgagctgg cccgcatcaa tggcgacctg ggccgcctgg gcggcctgct gaaactctgg
     8881 ctcaccgacg acccgcgcac ggcgcggttc ggtgatgcca cgatcctcgc cctgctggcg
     8941 aagatcgaag agaagcagga cgagcttggc aaggtcatga tgggcgtggt ccgcccgagg
     9001 gcagagccat gactttttta gccgctaaaa cggccggggg gtgcgcgtga ttgccaagca
     9061 cgtccccatg cgctccatca agaagagcga cttcgcggag ctggtgaagt acatcaccga
     9121 cgagcaaggc aagaccgatc gggccc
//
