LOCUS       Exported                3354 bp ds-DNA     circular UNA 03-MAR-2009
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Feb 17, 2021 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     rep_origin      complement(76)
                     /direction=LEFT
                     /label=ORI
                     /note="RNaseH cleavage point"
                     /note="/vntifkey=33"
     primer_bind     184..203
                     /label=JS836
     misc_feature    324..801
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=FCY1 selection (502bp linear)Fragment: from 
                     BpiI
                     cut at 10^11 to BpiI cut at 488^489, 478bpOrientation: 
                     originalPlus strand 5':   no changePlus strand 3':   no 
                     changeMinus strand 5':  no changeMinus strand 3':  no 
                     change"
     primer_bind     324..342
                     /label=JS2286
     CDS             325..798
                     /codon_start=1
                     /label=FCY1
                     /translation="MVTGGMASKWDQKGMDIAYEEAALGYKEGGVPIGGCLINNKDGSV
                     LGRGHNMRFQKGSATLHGEISTLENCGRLEGKVYKDTTLYTTLSPCDMCTGAIIMYGIP
                     RCVVGENVNFKSKGEKYLQTRGHEVVVVDDERCKKIMKQFIDERPQDWFEDIGE"
     primer_bind     complement(779..805)
                     /label=JS2287
     misc_feature    802..1430
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=Ecoli UPP (653bp linear)Fragment: from BpiI 
                     cut
                     at 10^11 to BpiI cut at 639^640, 629bpOrientation: 
                     originalPlus strand 5':   no changePlus strand 3':   no 
                     changeMinus strand 5':  no changeMinus strand 3':  no 
                     change"
     primer_bind     802..828
                     /label=JS2288
     CDS             808..1428
                     /codon_start=1
                     /label=EcUPP
                     /translation="KIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEATAD
                     LETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMYRNE
                     ETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLVAAPE
                     GIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     primer_bind     complement(1406..1434)
                     /label=JS2289
     misc_feature    join(1431..3354,1..323)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pICH41308 [CDS1] (2.845bp circular)Target
                     vector: from BpiI cut at 921^922 to BpiI cut at 323^324, 
                     2.247bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     misc_feature    1431..1434
                     /label=GCTT
                     /note="/vntifkey=21"
     primer_bind     complement(1548..1565)
                     /label=JS837
     CDS             1661..2671
                     /codon_start=1
                     /label=Sm/Sp no DraIII
                     /note="/vntifkey=4"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     variation       2638
                     /label=G to A
                     /label=nonstandard type:  mutation
                     /note="/vntifkey=62"
     misc_feature    join(2831..3354,1..91)
                     /label=rep (pMB1)
                     /note="/vntifkey=21"
ORIGIN
        1 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
       61 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      121 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      181 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      241 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      301 tcattaatca ctctgtggtc tcaaatggtg acagggggaa tggcaagcaa gtgggatcag
      361 aagggtatgg acattgccta tgaggaggcg gccttaggtt acaaagaggg tggtgttcct
      421 attggcggat gtcttatcaa taacaaagac ggaagtgttc tcggtcgtgg tcacaacatg
      481 agatttcaaa agggatccgc cacactacat ggtgagatct ccactttgga aaactgtggg
      541 agattagagg gcaaagtgta caaagatacc actttgtata cgacgctgtc tccatgcgac
      601 atgtgtacag gtgccatcat catgtatggt attccacgct gtgttgtcgg tgagaacgtt
      661 aatttcaaaa gtaagggcga gaaatattta caaactagag gtcacgaggt tgttgttgtt
      721 gacgatgaga ggtgtaaaaa gatcatgaaa caatttatcg atgaaagacc tcaggattgg
      781 tttgaagata ttggtgaggc ttccgccaag atcgtggaag tcaaacaccc actcgtcaaa
      841 cacaagctgg gactgatgcg tgagcaagat atcagcacca agcgctttcg cgaactcgct
      901 tccgaagtgg gtagcctgct gacttacgaa gcgaccgccg acctcgaaac ggaaaaagta
      961 actatcgaag gctggaacgg cccggtagaa atcgaccaga tcaaaggtaa gaaaattacc
     1021 gttgtgccaa ttctgcgtgc gggtcttggt atgatggacg gtgtgctgga aaacgttccg
     1081 agcgcgcgca tcagcgttgt cggtatgtac cgtaatgaag aaacgctgga gccggtaccg
     1141 tacttccaga aactggtttc taacatcgat gagcgtatgg cgctgatcgt tgacccaatg
     1201 ctggcaaccg gtggttccgt tatcgcgacc atcgacctgc tgaaaaaagc gggctgcagc
     1261 agcatcaaag ttctggtgct ggtagctgcg ccagaaggta tcgctgcgct ggaaaaagcg
     1321 cacccggacg tcgaactgta taccgcatcg attgatcagg gactgaacga gcacggatac
     1381 attattccgg gcctcggcga tgccggtgac aaaatctttg gtacgaaata gctttgagac
     1441 cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     1501 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     1561 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1621 accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc
     1681 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     1741 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     1801 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     1861 taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     1921 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     1981 cggctccgca gtggatggcg gcctgaagcc acacagcgat attgatttgc tggttacggt
     2041 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     2101 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     2161 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     2221 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     2281 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     2341 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     2401 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     2461 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     2521 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     2581 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc attacgtaaa
     2641 aggcgagatc accaaggtag tcggcaaata actgtcagac caagtttact catatatact
     2701 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     2761 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2821 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2881 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2941 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     3001 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3061 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3121 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3181 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3241 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3301 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atag
//
