LOCUS       Exported                6478 bp ds-DNA     circular SYN 25-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 25, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     unsure          1..141
                     /label=L-ITR
                     /note="/vntifkey=21"
     promoter        156..597
                     /label=hSyn promoter
     misc_feature    446..469
                     /label=ColonyF_hSyn
     misc_feature    568..626
                     /label=138_F_hSyn_GRAB
     CDS             598..2580
                     /codon_start=1
                     /label=ATP1.0
                     /translation="METDTLLLWVLLLWVPGSTGDTSLCEKVGTTEVLWPAVPNGTDAA
                     FLAGPGSSWGNSTVASTAAVSSSFKCALTKTGFQFYYLPAVYILVFIIGFLGNSVAIWM
                     FVFHMKPWSGISVYMFNLALADFLYVLTLPALIFYYFNKTDWIFGDAMCKLQRFIFHVN
                     LYGSILFLTCISAHRYSGVVYPLKSLGRLKKKNAICISVLVWLIVVVAISPILFYSGTG
                     VRKNKTITCYDTTSDEYLRSYFIYSMCTTVAMFCVPLVLILGCYGLIVRALIYPAPAGP
                     RDTDALDLEEGGNVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGPVLL
                     PDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVRKGEELFT
                     GVVPILVELDGDVNGHKFSVSGEGEGDATEGKLTLKFICTTGKLPVPWPTLVTTLTYGV
                     QCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKG
                     IDFKEDGNILGHKLEYNTGAAARWRGRSIYLVIIVLTVFAVSYIPFHVMKTMNLRARLD
                     FQTPAMCAFNDRVYATYQVTRGLASLNSCVDPILYFLAGDTFRRRLSRATRKASRRSEA
                     NLQSKSEDMTLNILPEFKQNGDTSL"
     misc_feature    complement(1810..1831)
                     /label=GFPreverse
     unsure          2595..3203
                     /label=WPRE
                     /note="/vntifkey=21"
     misc_feature    complement(2680..2697)
                     /label=pWPTRev
     misc_feature    complement(2680..2697)
                     /label=pWPTRev
     polyA_signal    3223..3701
                     /label=hGH polyA
                     /label=hGH polyA
                     /label=PolyA signal
                     /note="/vntifkey=25"
     unsure          3741..3881
                     /label=R-ITR
                     /note="/vntifkey=21"
     rep_origin      3973..4279
                     /label=f1 Origin
                     /label=f1 Origin
                     /label=Replication origin
                     /note="/vntifkey=33"
     unsure          4798..5655
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      5806..6473
                     /label=pUC ori
                     /label=pUC ori
                     /label=Replication origin
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatgag gcggggtggg ggtgcctacc
      181 tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc
      241 ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt
      301 cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca
      361 ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg
      421 gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg
      481 ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg
      541 tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg tgccaccatg
      601 gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac
      661 acaagtttgt gcgaaaaagt tggcaccacc gaggtgctgt ggccggctgt ccccaacggg
      721 acggacgctg ccttcctggc cggtccgggt tcgtcctggg ggaacagcac ggtcgcctcc
      781 actgccgccg tctcctcgtc gttcaaatgc gccttgacca agacgggctt ccagttttac
      841 tacctgccgg ctgtctacat cttggtattc atcatcggct tcctgggcaa cagcgtggcc
      901 atctggatgt tcgtcttcca catgaagccc tggagcggca tctccgtgta catgttcaat
      961 ttggctctgg ccgacttctt gtacgtgctg actctgccag ccctgatctt ctactacttc
     1021 aataaaacag actggatctt cggggatgcc atgtgtaaac tgcagaggtt catctttcat
     1081 gtgaacctct atggcagcat cttgtttctg acatgcatca gtgcccaccg gtacagcggt
     1141 gtggtgtacc ccctcaagtc cctgggccgg ctcaaaaaga agaatgcgat ctgtatcagc
     1201 gtgctggtgt ggctcattgt ggtggtggcg atctccccca tcctcttcta ctcaggtacc
     1261 ggggtccgca aaaacaaaac catcacctgt tacgacacca cctcagacga gtacctgcga
     1321 agttatttca tctacagcat gtgcacgacc gtggccatgt tctgtgtccc cttggtgctg
     1381 attctgggct gttacggatt aattgtgaga gctttgattt accccgcgcc ggccgggccg
     1441 cgcgacaccg acgcgctgga cctggaggag ggaggaaacg tctatatcaa ggccgacaag
     1501 cagaagaacg gcatcaaggc gaacttccac atccgccaca acatcgagga cggcggcgtg
     1561 cagctcgcct accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1621 gacaaccact acctgagcgt gcagtccaaa ctttcgaaag accccaacga gaagcgcgat
     1681 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1741 tacaagggcg gtaccggagg gagcatggtg agaaagggcg aggagctgtt caccggggtg
     1801 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1861 gagggtgagg gcgatgccac cgagggcaag ctgaccctga agttcatctg caccaccggc
     1921 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     1981 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     2041 tacatccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     2101 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     2161 gaggacggca acatcctggg gcacaagctg gagtacaaca ccggagcagc agcacgctgg
     2221 cgcgggcggt cgatttacct ggtaatcatt gtactgactg tttttgctgt gtcttacatc
     2281 cctttccatg tgatgaaaac gatgaacttg agggcccggc ttgattttca gaccccagca
     2341 atgtgtgctt tcaatgacag ggtttatgcc acgtatcagg tgacaagagg tctagcaagt
     2401 ctcaacagtt gtgtggaccc cattctctat ttcttggcgg gagatacttt cagaaggaga
     2461 ctctcccgag ccacaaggaa agcttctaga agaagtgagg caaatttgca atccaagagt
     2521 gaagacatga ccctcaatat tttacctgag ttcaagcaga atggagatac aagcctgtaa
     2581 gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     2641 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     2701 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     2761 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     2821 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     2881 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     2941 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     3001 tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg cgggacgtcc
     3061 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3121 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3181 gccgcctccc cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg catccctgtg
     3241 acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg
     3301 tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg
     3361 ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg
     3421 gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg
     3481 cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat
     3541 gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg
     3601 ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc
     3661 tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgattttg taggtaacca
     3721 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     3781 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     3841 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     3901 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     3961 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4021 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4081 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4141 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     4201 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4261 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
     4321 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4381 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     4441 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     4501 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     4561 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     4621 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     4681 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4741 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4801 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4861 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4921 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     4981 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5041 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5101 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5161 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5221 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5281 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5341 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5401 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5461 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5521 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5581 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5641 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5701 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5761 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5821 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5881 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5941 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     6001 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6061 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6121 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6181 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6241 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6301 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6361 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6421 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
