LOCUS       Exported                7104 bp ds-DNA     circular SYN 25-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 25, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7104
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     unsure          1..141
                     /label=L-ITR
                     /note="/vntifkey=21"
     promoter        156..1223
                     /label=GfaABC1D Promoter
     CDS             1224..3206
                     /codon_start=1
                     /label=ATP1.0
                     /translation="METDTLLLWVLLLWVPGSTGDTSLCEKVGTTEVLWPAVPNGTDAA
                     FLAGPGSSWGNSTVASTAAVSSSFKCALTKTGFQFYYLPAVYILVFIIGFLGNSVAIWM
                     FVFHMKPWSGISVYMFNLALADFLYVLTLPALIFYYFNKTDWIFGDAMCKLQRFIFHVN
                     LYGSILFLTCISAHRYSGVVYPLKSLGRLKKKNAICISVLVWLIVVVAISPILFYSGTG
                     VRKNKTITCYDTTSDEYLRSYFIYSMCTTVAMFCVPLVLILGCYGLIVRALIYPAPAGP
                     RDTDALDLEEGGNVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGPVLL
                     PDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVRKGEELFT
                     GVVPILVELDGDVNGHKFSVSGEGEGDATEGKLTLKFICTTGKLPVPWPTLVTTLTYGV
                     QCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKG
                     IDFKEDGNILGHKLEYNTGAAARWRGRSIYLVIIVLTVFAVSYIPFHVMKTMNLRARLD
                     FQTPAMCAFNDRVYATYQVTRGLASLNSCVDPILYFLAGDTFRRRLSRATRKASRRSEA
                     NLQSKSEDMTLNILPEFKQNGDTSL"
     misc_feature    complement(3207..3241)
                     /label=R1_AAV_GATP
     misc_feature    complement(3207..3241)
                     /label=R1_AAV_GATP
     unsure          3221..3829
                     /label=WPRE
                     /note="/vntifkey=21"
     misc_feature    complement(3306..3323)
                     /label=pWPTRev
     polyA_signal    3849..4327
                     /label=hGH polyA
                     /label=hGH polyA
                     /label=PolyA signal
                     /note="/vntifkey=25"
     unsure          4367..4507
                     /label=R-ITR
                     /note="/vntifkey=21"
     rep_origin      4599..4905
                     /label=f1 Origin
                     /label=f1 Origin
                     /label=Replication origin
                     /note="/vntifkey=33"
     unsure          5424..6281
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      6432..7099
                     /label=pUC ori
                     /label=pUC ori
                     /label=Replication origin
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgttgtag ttaatgatta acccgccatg
      181 ctacttatct acgtagccat gctctaggaa gatctaacat atcctggtgt ggagtagggg
      241 acgctgctct gacagaggct cgggggcctg agctggctct gtgagctggg gaggaggcag
      301 acagccaggc cttgtctgca agcagacctg gcagcattgg gctggccgcc ccccagggcc
      361 tcctcttcat gcccagtgaa tgactcacct tggcacagac acaatgttcg gggtgggcac
      421 agtgcctgct tcccgccgca ccccagcccc cctcaaatgc cttccgagaa gcccattgag
      481 cagggggctt gcattgcacc ccagcctgac agcctggcat cttgggataa aagcagcaca
      541 gccccctagg ggctgccctt gctgtgtggc gccaccggcg gtggagaaca aggctctatt
      601 cagcctgtgc ccaggaaagg ggatcagggg atgcccaggc atggacagtg ggtggcaggg
      661 ggggagagga gggctgtctg cttcccagaa gtccaaggac acaaatgggt gaggggagag
      721 ctctccccat agctgggctg cggcccaacc ccaccccctc aggctatgcc agggggtgtt
      781 gccaggggca cccgggcatc gccagtctag cccactcctt cataaagccc tcgcatccca
      841 ggagcgagca gagccagagc aggttggaga ggagacgcat cacctccgct gctcgcaagc
      901 tttattgcgg tagtttatca cagttaaatt gctaacgcag tcagtgcttc tgacacaaca
      961 gtctcgaact taagctgcag aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt
     1021 acaagacagg tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg
     1081 cgtttctgat aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt
     1141 gtccactccc agttcaatta cagctcttaa ggctagagta cttaatacga ctcactatag
     1201 gctagcctcg agaattcgcc accatggaga cagacacact cctgctatgg gtactgctgc
     1261 tctgggttcc aggttccact ggtgacacaa gtttgtgcga aaaagttggc accaccgagg
     1321 tgctgtggcc ggctgtcccc aacgggacgg acgctgcctt cctggccggt ccgggttcgt
     1381 cctgggggaa cagcacggtc gcctccactg ccgccgtctc ctcgtcgttc aaatgcgcct
     1441 tgaccaagac gggcttccag ttttactacc tgccggctgt ctacatcttg gtattcatca
     1501 tcggcttcct gggcaacagc gtggccatct ggatgttcgt cttccacatg aagccctgga
     1561 gcggcatctc cgtgtacatg ttcaatttgg ctctggccga cttcttgtac gtgctgactc
     1621 tgccagccct gatcttctac tacttcaata aaacagactg gatcttcggg gatgccatgt
     1681 gtaaactgca gaggttcatc tttcatgtga acctctatgg cagcatcttg tttctgacat
     1741 gcatcagtgc ccaccggtac agcggtgtgg tgtaccccct caagtccctg ggccggctca
     1801 aaaagaagaa tgcgatctgt atcagcgtgc tggtgtggct cattgtggtg gtggcgatct
     1861 cccccatcct cttctactca ggtaccgggg tccgcaaaaa caaaaccatc acctgttacg
     1921 acaccacctc agacgagtac ctgcgaagtt atttcatcta cagcatgtgc acgaccgtgg
     1981 ccatgttctg tgtccccttg gtgctgattc tgggctgtta cggattaatt gtgagagctt
     2041 tgatttaccc cgcgccggcc gggccgcgcg acaccgacgc gctggacctg gaggagggag
     2101 gaaacgtcta tatcaaggcc gacaagcaga agaacggcat caaggcgaac ttccacatcc
     2161 gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag aacaccccca
     2221 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcgtgcag tccaaacttt
     2281 cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     2341 ggatcactct cggcatggac gagctgtaca agggcggtac cggagggagc atggtgagaa
     2401 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     2461 acggccacaa gttcagcgtg tccggcgagg gtgagggcga tgccaccgag ggcaagctga
     2521 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     2581 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     2641 tcttcaagtc cgccatgccc gaaggctaca tccaggagcg caccatcttc ttcaaggacg
     2701 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     2761 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     2821 acaacaccgg agcagcagca cgctggcgcg ggcggtcgat ttacctggta atcattgtac
     2881 tgactgtttt tgctgtgtct tacatccctt tccatgtgat gaaaacgatg aacttgaggg
     2941 cccggcttga ttttcagacc ccagcaatgt gtgctttcaa tgacagggtt tatgccacgt
     3001 atcaggtgac aagaggtcta gcaagtctca acagttgtgt ggaccccatt ctctatttct
     3061 tggcgggaga tactttcaga aggagactct cccgagccac aaggaaagct tctagaagaa
     3121 gtgaggcaaa tttgcaatcc aagagtgaag acatgaccct caatatttta cctgagttca
     3181 agcagaatgg agatacaagc ctgtaagaat tcgatatcaa gcttatcgat aatcaacctc
     3241 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     3301 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     3361 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     3421 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     3481 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     3541 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     3601 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctatgttg
     3661 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     3721 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     3781 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg
     3841 agagatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt
     3901 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga
     3961 ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt
     4021 tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc
     4081 acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc
     4141 tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg
     4201 gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat
     4261 ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc
     4321 tgtccttctg attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg
     4381 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
     4441 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
     4501 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     4561 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4621 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4681 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4741 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
     4801 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4861 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4921 cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4981 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
     5041 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     5101 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     5161 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     5221 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     5281 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5341 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5401 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5461 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5521 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5581 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5641 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5701 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5761 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5821 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5881 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5941 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6001 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6061 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6121 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6181 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6241 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6301 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6361 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6421 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6481 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6541 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6601 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6661 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6721 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6781 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6841 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6901 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6961 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7021 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7081 tttgctggcc ttttgctcac atgt
//
