LOCUS       Exported                7544 bp ds-DNA     circular SYN 18-MAY-2017
DEFINITION  pcDNA5-TO-DsRed.seq.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 25, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-Forward
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(924..941)
                     /label=pcDNA5-TO-BssHII_R
     primer_bind     927..945
                     /label=NheI-Vpr-mRuby3-IN_F
     primer_bind     942..967
                     /label=pcDNA5-TO-BssHII_F
     CDS             974..1261
                     /codon_start=1
                     /product="Appears to be from YU-2 strain"
                     /label=Vpr
                     /translation="MEQAPEDQGPQREPHNEWTLELLEELKREAVRHFPRPWLHGLGQH
                     IYETYGDTWAGVEAIIRILQQLLFIHFRIGCQHSRIGIIQQRRARNGASRS"
     misc_feature    1283..1321
                     /label=RT-IN PR Cleavage Site
     misc_feature    1283..1303
                     /label=RT C-Term
     misc_feature    1304..1321
                     /label=IN N-Term
     CDS             1322..2032
                     /label=mRuby3
     primer_bind     1850..1870
                     /label=mRuby-177-F
     misc_feature    2069..2935
                     /label=HIV-1 Integrase
                     /note="Appears to be from SG-3 strain"
     primer_bind     complement(2123..2142)
                     /label=LAI RT-R
     primer_bind     2588..2606
                     /label=NL43_6bF
     misc_feature    2620..2737
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2625..2642
                     /label=NL4-cPPT_F
     primer_bind     2769..2787
                     /label=iSCA_F
     primer_bind     complement(2810..2836)
                     /label=BAL_5R
     primer_bind     complement(2881..2898)
                     /label=iSCA_R
     primer_bind     complement(2939..2956)
                     /label=pcDNA5-TO-SbfI_R
     primer_bind     complement(2946..2963)
                     /label=Vpr-mRuby3-IN-XhoI_R
     primer_bind     2957..2976
                     /label=pcDNA5-TO-SbfI_F
     primer_bind     complement(2966..2983)
                     /label=BGHR
     polyA_signal    2972..3196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3242..3670
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3684..4013
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3864..3999
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4018..4036
                     /label=AgeI-HygR_F
     CDS             4062..5087
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(5122..5139)
                     /label=HygR-SbfI_R
     polyA_signal    5217..5338
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5387..5403)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5411..5427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5435..5465)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5480..5501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5789..6377)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6548..7408)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7409..7513)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgct accatggaac aagccccaga agaccaaggg ccacagaggg agccgcacaa
     1021 tgaatggaca ctagagcttt tagaggagct taagagagaa gctgttagac attttcctag
     1081 gccatggcta catggcttag gacaacatat ctatgaaact tatggagata cttgggcagg
     1141 agtggaagcc ataataagaa ttctgcaaca actgctgttt attcatttca gaattgggtg
     1201 tcaacatagc agaataggca ttattcaaca gaggagagca agaaatggag ccagtagatc
     1261 agatctagta gataaattag gtgctggtat ccgtaaagta ctatttttag atggaataga
     1321 tatggtgtct aagggcgaag agctgatcaa ggaaaatatg cgtatgaagg tggtcatgga
     1381 aggttcggtc aacggccacc aattcaaatg cacaggtgaa ggagaaggca gaccgtacga
     1441 gggaactcaa accatgagga tcaaagtcat cgagggagga cccctgccat ttgcctttga
     1501 cattcttgcc acgtcgttca tgtatggcag ccgtactttt atcaagtacc cggccgacat
     1561 ccctgatttc tttaaacagt cctttcctga gggttttact tgggaaagag ttacgagata
     1621 cgaagatggt ggagtcgtca ccgtcacgca ggacaccagc cttgaggatg gcgagctcgt
     1681 ctacaacgtc aaggtcagag gggtaaactt tccctccaat ggtcccgtga tgcagaagaa
     1741 gaccaagggt tgggagccta atacagagat gatgtatcca gcagatggtg gtctgagagg
     1801 atacactgac atcgcactga aagttgatgg tggtggccat ctgcactgca acttcgtgac
     1861 aacttacagg tcaaaaaaga ccgtcgggaa catcaagatg cccggtgtcc atgccgttga
     1921 tcaccgcctg gaaaggatcg aggagagtga caatgaaacc tacgtagtgc aacgcgaagt
     1981 ggcagttgcc aaatacagca accttggtgg tggcatggac gagctgtaca agggatccgc
     2041 tggctccgct gctggttctg gcgaattctt tttagatgga atagataagg cccaagaaga
     2101 acatgagaaa tatcacagta attggagagc catggctagt gattttaact taccacctgt
     2161 agtagcaaaa gaaatagtag ccagctgtga taaatgtcag ctaaaaggag aagccatgca
     2221 tggacaagta gactgtagtc caggaatatg gcaactagat tgcacacatc tagaaggaaa
     2281 aattatcctg gtggcggttc atgtagccag tggatatata gaagcagaag ttattccagc
     2341 agagacaggg caggaaacag catactttct cttaaaatta gcaggaagat ggccagtaaa
     2401 aacaatacat acagacaatg gcagcaattt caccagtacc acggttaagg ccgcctgttg
     2461 gtgggcaggg atcaagcagg aatttggcat tccctacaat ccccaaagtc aaggagtagt
     2521 agaatctatg aataaagaat taaagaaaat tataggacag gtaagagatc aggctgaaca
     2581 tcttaaaaca gcagtacaaa tggcagtatt tatccacaat tttaaaagaa aaggggggat
     2641 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2701 agaactacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacaacaa
     2761 agatccactt tggaaaggac cagcaaagct tctctggaaa ggtgaagggg cagtagtaat
     2821 acaagataat agtgacataa aagtagtgcc aagaagaaaa gcaaagatca ttagagatta
     2881 tggaaaacag atggcaggtg atgattgtgt ggcaagtaga caggatgagg attgaaccgc
     2941 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     3001 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     3061 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     3121 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     3181 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     3241 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     3301 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     3361 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     3421 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     3481 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     3541 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     3601 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     3661 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     3721 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     3781 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3841 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     3901 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     3961 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     4021 cgggagcttg tatatccatt ttcggatctg atcagcacgt gatgaaaaag cctgaactca
     4081 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     4141 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     4201 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     4261 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     4321 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     4381 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     4441 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     4501 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     4561 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     4621 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     4681 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     4741 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     4801 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     4861 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     4921 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     4981 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     5041 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac gtgctacgag
     5101 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     5161 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     5221 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5281 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     5341 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     5401 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     5461 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     5521 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     5581 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5641 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5701 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5761 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5821 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5881 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5941 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6001 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6061 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6121 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6181 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     6241 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6301 gcaaacaaac caccgctggt agcggttggt tttttgtttg caagcagcag attacgcgca
     6361 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6421 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6481 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6541 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6601 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6661 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6721 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6781 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6841 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6901 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6961 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7021 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7081 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7141 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7201 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7261 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7321 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7381 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7441 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7501 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
