LOCUS       Exported               18313 bp ds-DNA     circular SYN 25-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18313)
  AUTHORS   Satoshi
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 25, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..18313
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1187..1816
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2250..3317
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3383..3577
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3921..4061
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      4247..4835
                     /label=pUC19 ori
     CDS             complement(5079..5870)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_signal     6398..6422
                     /label=LB
     protein_bind    6599..6620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6635..6665
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6673..6689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6697..6713
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6728..7527
                     /label=EC1.2en
     misc_feature    7532..8079
                     /label=EC1.1p
     CDS             8089..8154
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             8161..8181
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8206..12306
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             12307..12354
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      12362..12996
                     /label=rbcS-E9t
     protein_bind    13022..13146
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        13171..13201
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             13255..13935
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             14255..14560
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(14601..14725)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        14838..16219
                     /label=OLE1prom (-1382)
                     /note="As in pEDO97 (FastRed cassette)"
     misc_feature    16220..16978
                     /label=OLE1genome
                     /note="As in pEDO97 (FastRed cassette)"
     CDS             16985..17704
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      17705..17954
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(17964..17980)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    18176..18200
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
     2281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     2341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     2401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     2461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     2521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     2581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     2641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     2701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     2761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     2821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     2881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     2941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     3001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     3061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     3121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     3181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     3241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     3301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     3361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     3421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     3481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     3541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     3601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     3661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     3721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     3781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     3901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     3961 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     4021 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     4081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat
     4921 ctcccaattt gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct
     4981 gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta atcgcttgag ttaacgccgg
     5041 cgaagcggcg tcggcttgaa cgaatttcta gctagacatt atttgccgac taccttggtg
     5101 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
     5161 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
     5221 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
     5281 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg
     5341 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
     5401 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
     5461 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
     5521 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
     5581 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
     5641 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
     5701 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
     5761 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
     5821 actacctctg atagttgagt cgatacttcg gcgatcaccg cttcccccat gatgtttaac
     5881 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
     5941 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa
     6001 aaacatgtca taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     6061 ggtcaaggtt ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga
     6121 accgaacgag gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc
     6181 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
     6241 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
     6301 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
     6361 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
     6421 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
     6481 acgccgaatt gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6541 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6601 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6661 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
     6721 cgaattcgaa taaaagcatt tgcgtttggt ttatcattgc gtttatacaa ggacagagat
     6781 ccactgagct ggaatagctt aaaaccatta tcagaacaaa ataaaccatt ttttgttaag
     6841 aatcagagca tagtaaacaa cagaaacaac ctaagagagg taacttgtcc aagaagatag
     6901 ctaattatat ctattttata aaagttatca tagtttgtaa gtcacaaaag atgcaaataa
     6961 cagagaaact aggagacttg agaatataca ttcttgtata tttgtattcg agattgtgaa
     7021 aatttgacca taagtttaaa ttcttaaaaa gatatatctg atctaggtga tggttataga
     7081 ctgtaatttt accacatgtt taatgatgga tagtgacaca catgacacat cgacaacact
     7141 atagcatctt atttagatta caacatgaaa tttttctgta atacatgtct ttgtacataa
     7201 tttaaaagta attcctaaga aatatattta tacaaggagt ttaaagaaaa catagcataa
     7261 agttcaatga gtagtaaaaa ccatatacag tatatagcat aaagttcaat gagtttatta
     7321 caaaagcatt ggttcacttt ctgtaacacg acgttaaacc ttcgtctcca ataggagcgc
     7381 tactgattca acatgccaat atatactaaa tacgtttcta cagtcaaatg ctttaacgtt
     7441 tcatgattaa gtgactattt accgtcaatc ctttcccatt cctcccacta atccaacttt
     7501 ttaattactc ttaaatcacc actaagctag taacgcctat catgaattag ctctactaaa
     7561 tctagcaacc tttcaaattt gcagtattgc aggtgtctct gtgtctttaa aatagttgcc
     7621 ttatgatttc ttcggtttca agatgatcaa atagttatag atttcatgct cacacatgct
     7681 cattagatgt gtacatactt tacttaccca aatctatttt ctcgcaaaga ttttgatggt
     7741 aaagctgatt tggttctatt gaactaaatc aaacgagttt cagactgagt gattctaatc
     7801 cggcccatta gcccctaaac agacccacta attacgcagc ttttaataga gtaattacac
     7861 ctagtttacc cactaaacca ctaagcacta attatctcac aatctaatga gcttccctcg
     7921 taattacttg ggctttcact ctaccattta tttgtaacag tcaagtctct actgtctcta
     7981 tataaactct ctaaagttaa cacacaattc tcatcacaaa caaatcaacc aaagcaactt
     8041 ctactctttc ttctttcgac cttatcaatc tgttgagaaa tctagatgga ttacaaggac
     8101 cacgacgggg attacaagga ccacgacatt gattacaagg atgatgatga caagatggct
     8161 ccgaagaaga agaggaaggt tggcatccac ggggtgccag ctgctgacaa gaagtactcg
     8221 atcggcctcg atattgggac taactctgtt ggctgggccg tgatcaccga cgagtacaag
     8281 gtgccctcaa agaagttcaa ggtcctgggc aacaccgatc ggcattccat caagaagaat
     8341 ctcattggcg ctctcctgtt cgacagcggc gagacggctg aggctacgcg gctcaagcgc
     8401 accgcccgca ggcggtacac gcgcaggaag aatcgcatct gctacctgca ggagattttc
     8461 tccaacgaga tggcgaaggt tgacgattct ttcttccaca ggctggagga gtcattcctc
     8521 gtggaggagg ataagaagca cgagcggcat ccaatcttcg gcaacattgt cgacgaggtt
     8581 gcctaccacg agaagtaccc tacgatctac catctgcgga agaagctcgt ggactccaca
     8641 gataaggcgg acctccgcct gatctacctc gctctggccc acatgattaa gttcaggggc
     8701 catttcctga tcgaggggga tctcaacccg gacaatagcg atgttgacaa gctgttcatc
     8761 cagctcgtgc agacgtacaa ccagctcttc gaggagaacc ccattaatgc gtcaggcgtc
     8821 gacgcgaagg ctatcctgtc cgctaggctc tcgaagtctc ggcgcctcga gaacctgatc
     8881 gcccagctgc cgggcgagaa gaagaacggc ctgttcggga atctcattgc gctcagcctg
     8941 gggctcacgc ccaacttcaa gtcgaatttc gatctcgctg aggacgccaa gctgcagctc
     9001 tccaaggaca catacgacga tgacctggat aacctcctgg cccagatcgg cgatcagtac
     9061 gcggacctgt tcctcgctgc caagaatctg tcggacgcca tcctcctgtc tgatattctc
     9121 agggtgaaca ccgagattac gaaggctccg ctctcagcct ccatgatcaa gcgctacgac
     9181 gagcaccatc aggatctgac cctcctgaag gcgctggtca ggcagcagct ccccgagaag
     9241 tacaaggaga tcttcttcga tcagtcgaag aacggctacg ctgggtacat tgacggcggg
     9301 gcctctcagg aggagttcta caagttcatc aagccgattc tggagaagat ggacggcacg
     9361 gaggagctgc tggtgaagct caatcgcgag gacctcctga ggaagcagcg gacattcgat
     9421 aacggcagca tcccacacca gattcatctc ggggagctgc acgctatcct gaggaggcag
     9481 gaggacttct accctttcct caaggataac cgcgagaaga tcgagaagat tctgactttc
     9541 aggatcccgt actacgtcgg cccactcgct aggggcaact cccgcttcgc ttggatgacc
     9601 cgcaagtcag aggagacgat cacgccgtgg aacttcgagg aggtggtcga caagggcgct
     9661 agcgctcagt cgttcatcga gaggatgacg aatttcgaca agaacctgcc aaatgagaag
     9721 gtgctcccta agcactcgct cctgtacgag tacttcacag tctacaacga gctgactaag
     9781 gtgaagtatg tgaccgaggg catgaggaag ccggctttcc tgtctgggga gcagaagaag
     9841 gccatcgtgg acctcctgtt caagaccaac cggaaggtca cggttaagca gctcaaggag
     9901 gactacttca agaagattga gtgcttcgat tcggtcgaga tctctggcgt tgaggaccgc
     9961 ttcaacgcct ccctggggac ctaccacgat ctcctgaaga tcattaagga taaggacttc
    10021 ctggacaacg aggagaatga ggatatcctc gaggacattg tgctgacact cactctgttc
    10081 gaggaccggg agatgatcga ggagcgcctg aagacttacg cccatctctt cgatgacaag
    10141 gtcatgaagc agctcaagag gaggaggtac accggctggg ggaggctgag caggaagctc
    10201 atcaacggca ttcgggacaa gcagtccggg aagacgatcc tcgacttcct gaagagcgat
    10261 ggcttcgcga accgcaattt catgcagctg attcacgatg acagcctcac attcaaggag
    10321 gatatccaga aggctcaggt gagcggccag ggggactcgc tgcacgagca tatcgcgaac
    10381 ctcgctggct cgccagctat caagaagggg attctgcaga ccgtgaaggt tgtggacgag
    10441 ctggtgaagg tcatgggcag gcacaagcct gagaacatcg tcattgagat ggcccgggag
    10501 aatcagacca cgcagaaggg ccagaagaac tcacgcgaga ggatgaagag gatcgaggag
    10561 ggcattaagg agctggggtc ccagatcctc aaggagcacc cggtggagaa cacgcagctg
    10621 cagaatgaga agctctacct gtactacctc cagaatggcc gcgatatgta tgtggaccag
    10681 gagctggata ttaacaggct cagcgattac gacgtcgatc atatcgttcc acagtcattc
    10741 ctgaaggatg actccattga caacaaggtc ctcaccaggt cggacaagaa ccggggcaag
    10801 tctgataatg ttccttcaga ggaggtcgtt aagaagatga agaactactg gcgccagctc
    10861 ctgaatgcca agctgatcac gcagcggaag ttcgataacc tcacaaaggc tgagaggggc
    10921 gggctctctg agctggacaa ggcgggcttc atcaagaggc agctggtcga gacacggcag
    10981 atcactaagc acgttgcgca gattctcgac tcacggatga acactaagta cgatgagaat
    11041 gacaagctga tccgcgaggt gaaggtcatc accctgaagt caaagctcgt ctccgacttc
    11101 aggaaggatt tccagttcta caaggttcgg gagatcaaca attaccacca tgcccatgac
    11161 gcgtacctga acgcggtggt cggcacagct ctgatcaaga agtacccaaa gctcgagagc
    11221 gagttcgtgt acggggacta caaggtttac gatgtgagga agatgatcgc caagtcggag
    11281 caggagattg gcaaggctac cgccaagtac ttcttctact ctaacattat gaatttcttc
    11341 aagacagaga tcactctggc caatggcgag atccggaagc gccccctcat cgagacgaac
    11401 ggcgagacgg gggagatcgt gtgggacaag ggcagggatt tcgcgaccgt caggaaggtt
    11461 ctctccatgc cacaagtgaa tatcgtcaag aagacagagg tccagactgg cgggttctct
    11521 aaggagtcaa ttctgcctaa gcggaacagc gacaagctca tcgcccgcaa gaaggactgg
    11581 gatccgaaga agtacggcgg gttcgacagc cccactgtgg cctactcggt cctggttgtg
    11641 gcgaaggttg agaagggcaa gtccaagaag ctcaagagcg tgaaggagct gctggggatc
    11701 acgattatgg agcgctccag cttcgagaag aacccgatcg atttcctgga ggcgaagggc
    11761 tacaaggagg tgaagaagga cctgatcatt aagctcccca agtactcact cttcgagctg
    11821 gagaacggca ggaagcggat gctggcttcc gctggcgagc tgcagaaggg gaacgagctg
    11881 gctctgccgt ccaagtatgt gaacttcctc tacctggcct cccactacga gaagctcaag
    11941 ggcagccccg aggacaacga gcagaagcag ctgttcgtcg agcagcacaa gcattacctc
    12001 gacgagatca ttgagcagat ttccgagttc tccaagcgcg tgatcctggc cgacgcgaat
    12061 ctggataagg tcctctccgc gtacaacaag caccgcgaca agccaatcag ggagcaggct
    12121 gagaatatca ttcatctctt caccctgacg aacctcggcg cccctgctgc tttcaagtac
    12181 ttcgacacaa ctatcgatcg caagaggtac acaagcacta aggaggtcct ggacgcgacc
    12241 ctcatccacc agtcgattac cggcctctac gagacgcgca tcgacctgtc tcagctcggg
    12301 ggcgacaagc ggccagcggc gacgaagaag gcggggcagg cgaagaagaa gaagtgagct
    12361 cagagctttc gttcgtatca tcggtttcga caacgttcgt caagttcaat gcatcagttt
    12421 cattgcgcac acaccagaat cctactgagt ttgagtatta tggcattggg aaaactgttt
    12481 ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg
    12541 aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt
    12601 ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa
    12661 gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg
    12721 aagttaatat gaggagtaaa acacttgtag ttgtaccatt atgcttattc actaggcaac
    12781 aaatatattt tcagacctag aaaagctgca aatgttactg aatacaagta tgtcctcttg
    12841 tgttttagac atttatgaac tttcctttat gtaattttcc agaatccttg tcagattcta
    12901 atcattgctt tataattata gttatactca tggatttgta gttgagtatg aaaatatttt
    12961 ttaatgcatt ttatgacttg ccaattgatt gacaacgagc tccctaggcc tgttatccct
    13021 aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
    13081 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
    13141 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat
    13201 gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag
    13261 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
    13321 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
    13381 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
    13441 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg
    13501 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
    13561 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
    13621 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
    13681 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
    13741 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
    13801 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
    13861 agaatgctta atgaattaca acagtactgc gatgagtggc aggcggggcg taatctagag
    13921 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
    13981 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
    14041 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
    14101 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
    14161 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
    14221 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
    14281 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
    14341 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga
    14401 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
    14461 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
    14521 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
    14581 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
    14641 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
    14701 gttcagcttt cttgtacaaa gtggttcgat aattccgatc cagcctaggc ccgggcctga
    14761 ggacgcgtcc atggttaatt aagacgtccg gaccgactag tggatcctct agagtcgacc
    14821 tgcaggcatg caagcttctt caagtgtatg taggtatagt aacatgaaca agaacagact
    14881 caagtacaag atcgcatacg aaaatggaaa tggcaatgtc acttccacat aatcaaacac
    14941 gaatcctcat atcaacaagg cctgagattc taactagctc ataacaactt agccaatagt
    15001 tacttgagac taccaaatgt atgtagaact aaagactaag ggacagagag ttcgtctaaa
    15061 caggtgaatc tagtcgttgt tatctaataa acaattcagc cccaaatgca gaacacacat
    15121 agagctctct attgattcaa attacgatct gatactgata acgtctagat ttttagggtt
    15181 aaagcaatca atcacctgac gattcaaggt ggttggatca tgacgattcc agaaaacatc
    15241 aagcaagctc tcaaagctac actctttggg atcatactga actctaacaa cctcgttatg
    15301 tcccgtagtg ccagtacaga catcctcgta actcggattg tgcacgatgc catggctata
    15361 cccaacctcg gtcttggtca caccaggaac tctctggtaa gctagctcca ctccccagaa
    15421 acaaccggcg ccaaattgcg cgaattgctg acctgaagac ggaacatcat cgtcgggtcc
    15481 ttgggcgatt gcggcggaag atgggtcagc ttgggcttga ggacgagacc cgaatccgag
    15541 tctgttgaaa aggttgttca ttggggattt gtatacggag attggtcgtc gagaggtttg
    15601 agggaaagga caaatgggtt tggctctgga gaaagagagt gcggctttag agagagaatt
    15661 gagaggttta gagagagatg cggcggcgat gagcggagga gagacgacga ggacctgcat
    15721 tatcaaagca gtgacgtggt gaaatttgga acttttaaga ggcagataga tttattattt
    15781 gtatccattt tcttcattgt tctagaatgt cgcggaacaa attttaaaac taaatcctaa
    15841 atttttctaa ttttgttgcc aatagtggat atgtgggccg tatagaagga atctattgaa
    15901 ggcccaaacc catactgacg agcccaaagg ttcgttttgc gttttatgtt tcggttcgat
    15961 gccaacgcca cattctgagc taggcaaaaa acaaacgtgt ctttgaatag actcctctcg
    16021 ttaacacatg cagcggctgc atggtgacgc cattaacacg tggcctacaa ttgcatgatg
    16081 tctccattga cacgtgactt ctcgtctcct ttcttaatat atctaacaaa cactcctacc
    16141 tcttccaaaa tatatacaca tctttttgat caatctctca ttcaaaatct cattctctct
    16201 agtaaacaag aacaaaaaaa tggcggatac agctagagga acccatcacg atatcatcgg
    16261 cagagaccag tacccgatga tgggccgaga ccgagaccag taccagatgt ccggacgagg
    16321 atctgactac tccaagtcta ggcagattgc taaagctgca actgctgtca cagctggtgg
    16381 ttccctcctt gttctctcca gccttaccct tgttggaact gtcatagctt tgactgttgc
    16441 aacacctctg ctcgttatct tcagcccaat ccttgtcccg gctctcatca cagttgcact
    16501 cctcatcacc ggttttcttt cctctggagg gtttggcatt gccgctataa ccgttttctc
    16561 ttggatttac aagtaagcac acatttatca tcttacttca taattttgtg caatatgtgc
    16621 atgcatgtgt tgagccagta gctttggatc aatttttttg gtcgaataac aaatgtaaca
    16681 ataagaaatt gcaaattcta gggaacattt ggttaactaa atacgaaatt tgacctagct
    16741 agcttgaatg tgtctgtgta tatcatctat ataggtaaaa tgcttggtat gatacctatt
    16801 gattgtgaat aggtacgcaa cgggagagca cccacaggga tcagacaagt tggacagtgc
    16861 aaggatgaag ttgggaagca aagctcagga tctgaaagac agagctcagt actacggaca
    16921 gcaacatact ggtggggaac atgaccgtga ccgtactcgt ggtggccagc acactactat
    16981 gagcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
    17041 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
    17101 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
    17161 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
    17221 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
    17281 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
    17341 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
    17401 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
    17461 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
    17521 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
    17581 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
    17641 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
    17701 gtgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat
    17761 tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct
    17821 cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa
    17881 ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca
    17941 aatgtttgaa cgataagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac
    18001 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
    18061 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatga
    18121 gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca
    18181 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataac ggatatttaa
    18241 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
    18301 ccctcgggat caa
//
