LOCUS       Exported               16746 bp ds-DNA     circular SYN 25-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16746)
  AUTHORS   robertas
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 25, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16746
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      2541..3541
                     /label=pVS-1 ori
                     /note="pVS-1 ori"
     rep_origin      4247..4835
                     /label=pUC19 ori
                     /note="pUC19 ori"
     CDS             complement(5079..5870)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_signal     6398..6422
                     /label=LB
                     /note="LB"
     primer_bind     6697..6713
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6728..7699
                     /label=PcUbi(P)
                     /note="PcUbi(P)"
     misc_feature    7714..7721
                     /label=PcUbi(P)
     CDS             7728..10938
                     /label=SaCas9
     misc_feature    10945..10952
                     /label=pea 3A term
                     /label=pea\3A\term
                     /note="/vntifkey=21"
     misc_feature    10967..11435
                     /label=Pea3A(T)
                     /note="Pea3A(T)"
     protein_bind    11461..11585
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             11694..12374
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             12694..12999
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(13040..13164)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        13277..14658
                     /label=pOLE1 promoter
                     /note="OLE1prom (-1382)"
                     /note="As in pEDO97 (FastRed cassette)"
     misc_feature    14659..15417
                     /label=OLE1 gene
                     /note="OLE1genome"
                     /note="As in pEDO97 (FastRed cassette)"
     CDS             15418..16137
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    16138..16387
                     /label=NOST
                     /note="NOST"
     primer_bind     complement(16397..16413)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_signal     16609..16633
                     /label=RB
                     /note="RB"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
     2281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     2341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     2401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     2461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     2521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     2581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     2641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     2701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     2761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     2821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     2881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     2941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     3001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     3061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     3121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     3181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     3241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     3301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     3361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     3421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     3481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     3541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     3601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     3661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     3721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     3781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     3901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     3961 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     4021 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     4081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat
     4921 ctcccaattt gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct
     4981 gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta atcgcttgag ttaacgccgg
     5041 cgaagcggcg tcggcttgaa cgaatttcta gctagacatt atttgccgac taccttggtg
     5101 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
     5161 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
     5221 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
     5281 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg
     5341 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
     5401 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
     5461 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
     5521 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
     5581 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
     5641 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
     5701 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
     5761 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
     5821 actacctctg atagttgagt cgatacttcg gcgatcaccg cttcccccat gatgtttaac
     5881 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
     5941 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa
     6001 aaacatgtca taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     6061 ggtcaaggtt ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga
     6121 accgaacgag gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc
     6181 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
     6241 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
     6301 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
     6361 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
     6421 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
     6481 acgccgaatt gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6541 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6601 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6661 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
     6721 cgaattcaaa aattacggat atgaatatag gcatatccgt atccgaatta tccgtttgac
     6781 agctagcaac gattgtacaa ttgcttcttt aaaaaaggaa gaaagaaaga aagaaaagaa
     6841 tcaacatcag cgttaacaaa cggccccgtt acggcccaaa cggtcatata gagtaacggc
     6901 gttaagcgtt gaaagactcc tatcgaaata cgtaaccgca aacgtgtcat agtcagatcc
     6961 cctcttcctt caccgcctca aacacaaaaa taatcttcta cagcctatat atacaacccc
     7021 cccttctatc tctcctttct cacaattcat catctttctt tctctacccc caattttaag
     7081 aaatcctctc ttctcctctt cattttcaag gtaaatctct ctctctctct ctctctctgt
     7141 tattccttgt tttaattagg tatgtattat tgctagtttg ttaatctgct tatcttatgt
     7201 atgccttatg tgaatatctt tatcttgttc atctcatccg tttagaagct ataaatttgt
     7261 tgatttgact gtgtatctac acgtggttat gtttatatct aatcagatat gaatttcttc
     7321 atattgttgc gtttgtgtgt accaatccga aatcgttgat ttttttcatt taatcgtgta
     7381 gctaattgta cgtatacata tggatctacg tatcaattgt tcatctgttt gtgtttgtat
     7441 gtatacagat ctgaaaacat cacttctctc atctgattgt gttgttacat acatagatat
     7501 agatctgtta tatcattttt tttattaatt gtgtatatat atatgtgcat agatctggat
     7561 tacatgattg tgattattta catgattttg ttatttacgt atgtatatat gtagatctgg
     7621 actttttgga gttgttgact tgattgtatt tgtgtgtgta tatgtgtgtt ctgatcttga
     7681 tatgttatgt atgtgcagcg aattcggcgc gcctgtgcag cgaattcggc gcgccatgaa
     7741 gaggaactac atcctcggac tcgatatcgg aatcacctct gtgggatacg gaatcatcga
     7801 ttacgagact agggatgtga tcgatgctgg tgtgagactc ttcaaagagg ctaacgttga
     7861 gaacaacgag ggaagaaggt ctaagagggg agctagaagg ctcaagagaa gaagaaggca
     7921 cagaatccag agggtgaaga agctcctctt cgattacaac ctcctcaccg atcactctga
     7981 gctttctgga atcaaccctt acgaggcaag agtgaaggga ctctctcaga agttgtctga
     8041 ggaagagttc tctgctgctt tgctccacct tgctaagaga aggggtgtgc ataacgtgaa
     8101 cgaggtggaa gaggataccg gaaacgagct ttctaccaaa gagcagatct ctaggaactc
     8161 taaggctctc gaagagaagt acgtggcaga gcttcagctt gagagactca agaaagatgg
     8221 tgaggtgagg ggatctatca acaggttcaa gacctctgat tacgtgaaag aagctaagca
     8281 gctcctcaag gtgcagaagg cttaccatca gctcgatcag tctttcatcg atacctacat
     8341 cgatcttctc gagactagaa ggacctacta cgagggacct ggtgaaggat ctccattcgg
     8401 atggaaggat atcaaagaat ggtacgagat gctcatggga cactgcactt acttccctga
     8461 agaactcaga tctgtgaagt acgcttacaa cgctgatctc tacaacgctc ttaacgatct
     8521 caacaacctc gtgatcacca gggatgagaa cgagaagctt gagtactacg agaagttcca
     8581 gatcatcgag aacgtgttca agcaaaagaa gaagcctacc ctcaagcaga tcgctaaaga
     8641 gatccttgtt aacgaggaag atatcaaggg atacagggtg acctctaccg gaaagcctga
     8701 gttcactaac ctcaaggttt accacgatat taaggatatt accgctagaa aagagattat
     8761 tgagaacgct gagcttctcg atcaaatcgc taagatcctt accatctacc agtcatctga
     8821 ggatatccaa gaggaattga ccaacctcaa ctcagagctt acccaagaag agatcgagca
     8881 gatcagtaac ttgaagggtt acaccggaac ccacaacctc tcactcaagg ctatcaacct
     8941 catcctcgat gagctttggc acaccaacga taaccagatc gcaatcttca acagactcaa
     9001 gctcgtgcct aagaaagtgg atctctctca gcagaaagag attcctacca ccctcgtgga
     9061 tgatttcatc ctctcacctg ttgtgaagag atctttcatc cagtctatca aggtgatcaa
     9121 cgctattatc aagaaatacg gactccctaa cgatatcatc atcgagttgg ctagggaaaa
     9181 gaactcaaag gatgctcaaa agatgatcaa cgagatgcag aagagaaaca ggcagaccaa
     9241 cgagaggatc gaagagatca tcaggaccac cggaaaagag aacgctaagt acctcattga
     9301 gaagatcaag ctccacgata tgcaagaggg aaagtgcctc tactctctcg aggctatccc
     9361 tcttgaggat ctccttaaca accctttcaa ctacgaagtg gatcacatca tccctagatc
     9421 tgtgtctttc gataactctt tcaacaacaa ggtgctcgtg aagcaagagg aaaactctaa
     9481 gaagggtaac aggacccctt tccagtacct ctcttcatct gattctaaga tctcatacga
     9541 gactttcaag aagcacatcc tcaacctcgc taagggaaag ggtaggatct ctaagacaaa
     9601 gaaagagtac cttctcgagg aaagggatat caacagattc tctgttcaga aggatttcat
     9661 caacagaaac cttgtggata ccaggtacgc taccaggggt cttatgaacc ttctcaggtc
     9721 ttacttcagg gtgaacaacc ttgatgtgaa ggtgaagtca atcaacggtg gattcacctc
     9781 attccttaga agaaaatgga agtttaagaa agagaggaac aagggttata agcaccacgc
     9841 tgaggatgct ctcattatcg ctaacgcaga tttcattttc aaagagtgga agaagttgga
     9901 taaggctaaa aaggtgatgg aaaaccagat gttcgaggaa aagcaggctg agtctatgcc
     9961 tgagatcgag actgagcaag agtacaaaga aatcttcatc acccctcacc agatcaagca
    10021 catcaaagat ttcaaggatt acaagtactc tcacagagtg gataagaagc caaacagaga
    10081 gcttatcaac gataccctct actcaaccag aaaggatgat aagggaaaca ccctcatcgt
    10141 gaacaatctc aacggactct acgataagga taacgataag ctcaagaagc tcattaacaa
    10201 gtctccagag aagttgctca tgtaccacca cgatcctcag acctaccaaa agctcaagct
    10261 catcatggaa cagtacggtg atgagaagaa ccctctctac aagtattacg aggaaaccgg
    10321 taactacctc accaagtact caaaaaagga taatggacct gtgatcaaga agattaagta
    10381 ctacggaaac aagctcaacg cacacctcga tatcaccgat gattacccta acagtagaaa
    10441 caaggtggtg aagctttcac tcaagcctta caggttcgat gtgtacctcg ataacggtgt
    10501 gtacaagttc gtgaccgtga agaacctcga tgttatcaaa aaagaaaact actatgaggt
    10561 taactctaag tgctacgagg aagctaagaa gttgaagaag atctctaacc aggcagagtt
    10621 cattgcttca ttctacaaca acgatcttat caagattaac ggtgagcttt acagggttat
    10681 cggagtgaac aacgatttgc tcaacaggat cgaggtgaac atgattgata tcacctacag
    10741 ggaatacctc gagaatatga acgataagag gcctcctaga atcatcaaga ctatcgcttc
    10801 taagacccag agtatcaaga agtactctac cgatatcctc ggaaacctct acgaggttaa
    10861 gtcaaagaag caccctcaga tcattaagaa gggatcaagg gctgatccta agaagaagag
    10921 gaaggtttga ggcgcgccga gctccaggcc tcggcgcgcc gagctccagg cctcccagct
    10981 ttcgtccgta tcatcggttt cgacaacgtt cgtcaagttc aatgcatcag tttcattgcc
    11041 cacacaccag aatcctacta agtttgagta ttatggcatt ggaaaagctg ttttcttcta
    11101 tcatttgttc tgcttgtaat ttactgtgtt ctttcagttt ttgttttcgg acatcaaaat
    11161 gcaaatggat ggataagagt taataaatga tatggtcctt ttgttcattc tcaaattatt
    11221 attatctgtt gtttttactt taatgggttg aatttaagta agaaaggaac taacagtgtg
    11281 atattaaggt gcaatgttag acatataaaa cagtctttca cctctctttg gttatgtctt
    11341 gaattggttt gtttcttcac ttatctgtgt aatcaagttt actatgagtc tatgatcaag
    11401 taattatgca atcaagttaa gtacagtata ggcttgagct ccctaggcct gttatcccta
    11461 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
    11521 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
    11581 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
    11641 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
    11701 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
    11761 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
    11821 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
    11881 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
    11941 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
    12001 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
    12061 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
    12121 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
    12181 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
    12241 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
    12301 gaatgcttaa tgaattacaa cagtactgcg atgagtggca ggcggggcgt aatctagagg
    12361 atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
    12421 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag
    12481 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca
    12541 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
    12601 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
    12661 gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa
    12721 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
    12781 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt cccccgtgaa
    12841 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
    12901 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
    12961 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
    13021 agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt
    13081 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
    13141 ttcagctttc ttgtacaaag tggttcgata attccgatcc agcctaggcc cgggcctgag
    13201 gacgcgtcca tggttaatta agacgtccgg accgactagt ggatcctcta gagtcgacct
    13261 gcaggcatgc aagcttcttc aagtgtatgt aggtatagta acatgaacaa gaacagactc
    13321 aagtacaaga tcgcatacga aaatggaaat ggcaatgtca cttccacata atcaaacacg
    13381 aatcctcata tcaacaaggc ctgagattct aactagctca taacaactta gccaatagtt
    13441 acttgagact accaaatgta tgtagaacta aagactaagg gacagagagt tcgtctaaac
    13501 aggtgaatct agtcgttgtt atctaataaa caattcagcc ccaaatgcag aacacacata
    13561 gagctctcta ttgattcaaa ttacgatctg atactgataa cgtctagatt tttagggtta
    13621 aagcaatcaa tcacctgacg attcaaggtg gttggatcat gacgattcca gaaaacatca
    13681 agcaagctct caaagctaca ctctttggga tcatactgaa ctctaacaac ctcgttatgt
    13741 cccgtagtgc cagtacagac atcctcgtaa ctcggattgt gcacgatgcc atggctatac
    13801 ccaacctcgg tcttggtcac accaggaact ctctggtaag ctagctccac tccccagaaa
    13861 caaccggcgc caaattgcgc gaattgctga cctgaagacg gaacatcatc gtcgggtcct
    13921 tgggcgattg cggcggaaga tgggtcagct tgggcttgag gacgagaccc gaatccgagt
    13981 ctgttgaaaa ggttgttcat tggggatttg tatacggaga ttggtcgtcg agaggtttga
    14041 gggaaaggac aaatgggttt ggctctggag aaagagagtg cggctttaga gagagaattg
    14101 agaggtttag agagagatgc ggcggcgatg agcggaggag agacgacgag gacctgcatt
    14161 atcaaagcag tgacgtggtg aaatttggaa cttttaagag gcagatagat ttattatttg
    14221 tatccatttt cttcattgtt ctagaatgtc gcggaacaaa ttttaaaact aaatcctaaa
    14281 tttttctaat tttgttgcca atagtggata tgtgggccgt atagaaggaa tctattgaag
    14341 gcccaaaccc atactgacga gcccaaaggt tcgttttgcg ttttatgttt cggttcgatg
    14401 ccaacgccac attctgagct aggcaaaaaa caaacgtgtc tttgaataga ctcctctcgt
    14461 taacacatgc agcggctgca tggtgacgcc attaacacgt ggcctacaat tgcatgatgt
    14521 ctccattgac acgtgacttc tcgtctcctt tcttaatata tctaacaaac actcctacct
    14581 cttccaaaat atatacacat ctttttgatc aatctctcat tcaaaatctc attctctcta
    14641 gtaaacaaga acaaaaaaat ggcggataca gctagaggaa cccatcacga tatcatcggc
    14701 agagaccagt acccgatgat gggccgagac cgagaccagt accagatgtc cggacgagga
    14761 tctgactact ccaagtctag gcagattgct aaagctgcaa ctgctgtcac agctggtggt
    14821 tccctccttg ttctctccag ccttaccctt gttggaactg tcatagcttt gactgttgca
    14881 acacctctgc tcgttatctt cagcccaatc cttgtcccgg ctctcatcac agttgcactc
    14941 ctcatcaccg gttttctttc ctctggaggg tttggcattg ccgctataac cgttttctct
    15001 tggatttaca agtaagcaca catttatcat cttacttcat aattttgtgc aatatgtgca
    15061 tgcatgtgtt gagccagtag ctttggatca atttttttgg tcgaataaca aatgtaacaa
    15121 taagaaattg caaattctag ggaacatttg gttaactaaa tacgaaattt gacctagcta
    15181 gcttgaatgt gtctgtgtat atcatctata taggtaaaat gcttggtatg atacctattg
    15241 attgtgaata ggtacgcaac gggagagcac ccacagggat cagacaagtt ggacagtgca
    15301 aggatgaagt tgggaagcaa agctcaggat ctgaaagaca gagctcagta ctacggacag
    15361 caacatactg gtggggaaca tgaccgtgac cgtactcgtg gtggccagca cactactatg
    15421 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
    15481 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
    15541 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
    15601 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
    15661 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
    15721 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
    15781 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
    15841 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
    15901 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
    15961 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
    16021 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
    16081 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtgatct
    16141 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
    16201 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
    16261 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
    16321 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
    16381 gaacgataag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
    16441 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
    16501 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgagcttgag
    16561 cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata
    16621 ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg
    16681 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
    16741 gatcaa
//
