LOCUS       Exported               18014 bp ds-DNA     circular SYN 25-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18014)
  AUTHORS   robertas
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 25, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..18014
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      2541..3541
                     /label=pVS-1 ori
                     /note="pVS-1 ori"
     rep_origin      4247..4835
                     /label=pUC19 ori
                     /note="pUC19 ori"
     CDS             complement(5079..5870)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_signal     6398..6422
                     /label=LB
                     /note="LB"
     primer_bind     6697..6713
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6734..7533
                     /label=EC1.2en
     misc_feature    7538..8085
                     /label=EC1.1p
     CDS             8105..12208
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    12259..12727
                     /label=Pea3A(T)
                     /note="Pea3A(T)"
     protein_bind    12753..12877
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             12986..13666
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             13986..14291
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(14332..14456)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        14569..15950
                     /label=pOLE1 promoter
                     /note="OLE1prom (-1382)"
                     /note="As in pEDO97 (FastRed cassette)"
     misc_feature    15951..16709
                     /label=OLE1 gene
                     /note="OLE1genome"
                     /note="As in pEDO97 (FastRed cassette)"
     exon            16716..17405
                     /label=tagRFP
                     /note="tagRFP"
                     /note="As in pEDO97 (FastRed cassette)"
     misc_feature    17406..17655
                     /label=NOST
                     /note="NOST"
     primer_bind     complement(17665..17681)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_signal     17877..17901
                     /label=RB
                     /note="RB"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
     2281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     2341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     2401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     2461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     2521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     2581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     2641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     2701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     2761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     2821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     2881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     2941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     3001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     3061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     3121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     3181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     3241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     3301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     3361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     3421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     3481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     3541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     3601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     3661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     3721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     3781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     3901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     3961 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     4021 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     4081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat
     4921 ctcccaattt gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct
     4981 gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta atcgcttgag ttaacgccgg
     5041 cgaagcggcg tcggcttgaa cgaatttcta gctagacatt atttgccgac taccttggtg
     5101 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
     5161 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
     5221 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
     5281 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg
     5341 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
     5401 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
     5461 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
     5521 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
     5581 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
     5641 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
     5701 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
     5761 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
     5821 actacctctg atagttgagt cgatacttcg gcgatcaccg cttcccccat gatgtttaac
     5881 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
     5941 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa
     6001 aaacatgtca taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     6061 ggtcaaggtt ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga
     6121 accgaacgag gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc
     6181 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
     6241 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
     6301 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
     6361 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
     6421 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
     6481 acgccgaatt gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6541 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6601 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6661 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
     6721 cgaattcggt accgaataaa agcatttgcg tttggtttat cattgcgttt atacaaggac
     6781 agagatccac tgagctggaa tagcttaaaa ccattatcag aacaaaataa accatttttt
     6841 gttaagaatc agagcatagt aaacaacaga aacaacctaa gagaggtaac ttgtccaaga
     6901 agatagctaa ttatatctat tttataaaag ttatcatagt ttgtaagtca caaaagatgc
     6961 aaataacaga gaaactagga gacttgagaa tatacattct tgtatatttg tattcgagat
     7021 tgtgaaaatt tgaccataag tttaaattct taaaaagata tatctgatct aggtgatggt
     7081 tatagactgt aattttacca catgtttaat gatggatagt gacacacatg acacatcgac
     7141 aacactatag catcttattt agattacaac atgaaatttt tctgtaatac atgtctttgt
     7201 acataattta aaagtaattc ctaagaaata tatttataca aggagtttaa agaaaacata
     7261 gcataaagtt caatgagtag taaaaaccat atacagtata tagcataaag ttcaatgagt
     7321 ttattacaaa agcattggtt cactttctgt aacacgacgt taaaccttcg tctccaatag
     7381 gagcgctact gattcaacat gccaatatat actaaatacg tttctacagt caaatgcttt
     7441 aacgtttcat gattaagtga ctatttaccg tcaatccttt cccattcctc ccactaatcc
     7501 aactttttaa ttactcttaa atcaccacta agctagtaac gcctatcatg aattagctct
     7561 actaaatcta gcaacctttc aaatttgcag tattgcaggt gtctctgtgt ctttaaaata
     7621 gttgccttat gatttcttcg gtttcaagat gatcaaatag ttatagattt catgctcaca
     7681 catgctcatt agatgtgtac atactttact tacccaaatc tattttctcg caaagatttt
     7741 gatggtaaag ctgatttggt tctattgaac taaatcaaac gagtttcaga ctgagtgatt
     7801 ctaatccggc ccattagccc ctaaacagac ccactaatta cgcagctttt aatagagtaa
     7861 ttacacctag tttacccact aaaccactaa gcactaatta tctcacaatc taatgagctt
     7921 ccctcgtaat tacttgggct ttcactctac catttatttg taacagtcaa gtctctactg
     7981 tctctatata aactctctaa agttaacaca caattctcat cacaaacaaa tcaaccaaag
     8041 caacttctac tctttcttct ttcgacctta tcaatctgtt gagaaggtac caattcggcg
     8101 cgccatggat aagaagtact ctatcggact cgatatcgga actaactctg tgggatgggc
     8161 tgtgatcacc gatgagtaca aggtgccatc taagaagttc aaggttctcg gaaacaccga
     8221 taggcactct atcaagaaaa accttatcgg tgctctcctc ttcgattctg gtgaaactgc
     8281 tgaggctacc agactcaaga gaaccgctag aagaaggtac accagaagaa agaacaggat
     8341 ctgctacctc caagagatct tctctaacga gatggctaaa gtggatgatt cattcttcca
     8401 caggctcgaa gagtcattcc tcgtggaaga agataagaag cacgagaggc accctatctt
     8461 cggaaacatc gttgatgagg tggcatacca cgagaagtac cctactatct accacctcag
     8521 aaagaagctc gttgattcta ctgataaggc tgatctcagg ctcatctacc tcgctctcgc
     8581 tcacatgatc aagttcagag gacacttcct catcgagggt gatctcaacc ctgataactc
     8641 tgatgtggat aagttgttca tccagctcgt gcagacctac aaccagcttt tcgaagagaa
     8701 ccctatcaac gcttcaggtg tggatgctaa ggctatcctc tctgctaggc tctctaagtc
     8761 aagaaggctt gagaacctca ttgctcagct ccctggtgag aagaagaacg gacttttcgg
     8821 aaacttgatc gctctctctc tcggactcac ccctaacttc aagtctaact tcgatctcgc
     8881 tgaggatgca aagctccagc tctcaaagga tacctacgat gatgatctcg ataacctcct
     8941 cgctcagatc ggagatcagt acgctgattt gttcctcgct gctaagaacc tctctgatgc
     9001 tatcctcctc agtgatatcc tcagagtgaa caccgagatc accaaggctc cactctcagc
     9061 ttctatgatc aagagatacg atgagcacca ccaggatctc acacttctca aggctcttgt
     9121 tagacagcag ctcccagaga agtacaaaga gattttcttc gatcagtcta agaacggata
     9181 cgctggttac atcgatggtg gtgcatctca agaagagttc tacaagttca tcaagcctat
     9241 cctcgagaag atggatggaa ccgaggaact cctcgtgaag ctcaatagag aggatcttct
     9301 cagaaagcag aggaccttcg ataacggatc tatccctcat cagatccacc tcggagagtt
     9361 gcacgctatc cttagaaggc aagaggattt ctacccattc ctcaaggata acagggaaaa
     9421 gattgagaag attctcacct tcagaatccc ttactacgtg ggacctctcg ctagaggaaa
     9481 ctcaagattc gcttggatga ccagaaagtc tgaggaaacc atcacccctt ggaacttcga
     9541 agaggtggtg gataagggtg ctagtgctca gtctttcatc gagaggatga ccaacttcga
     9601 taagaacctt ccaaacgaga aggtgctccc taagcactct ttgctctacg agtacttcac
     9661 cgtgtacaac gagttgacca aggttaagta cgtgaccgag ggaatgagga agcctgcttt
     9721 tttgtcaggt gagcaaaaga aggctatcgt tgatctcttg ttcaagacca acagaaaggt
     9781 gaccgtgaag cagctcaaag aggattactt caagaaaatc gagtgcttcg attcagttga
     9841 gatttctggt gttgaggata ggttcaacgc atctctcgga acctaccacg atctcctcaa
     9901 gatcattaag gataaggatt tcttggataa cgaggaaaac gaggatatct tggaggatat
     9961 cgttcttacc ctcaccctct ttgaagatag agagatgatt gaagaaaggc tcaagaccta
    10021 cgctcatctc ttcgatgata aggtgatgaa gcagttgaag agaagaagat acactggttg
    10081 gggaaggctc tcaagaaagc tcattaacgg aatcagggat aagcagtctg gaaagacaat
    10141 ccttgatttc ctcaagtctg atggattcgc taacagaaac ttcatgcagc tcatccacga
    10201 tgattctctc acctttaaag aggatatcca gaaggctcag gtttcaggac agggtgatag
    10261 tctccatgag catatcgcta acctcgctgg atctcctgca atcaagaagg gaatcctcca
    10321 gactgtgaag gttgtggatg agttggtgaa ggtgatggga aggcataagc ctgagaacat
    10381 cgtgatcgaa atggctagag agaaccagac cactcagaag ggacagaaga actctaggga
    10441 aaggatgaag aggatcgagg aaggtatcaa agagcttgga tctcagatcc tcaaagagca
    10501 ccctgttgag aacactcagc tccagaatga gaagctctac ctctactacc tccagaacgg
    10561 aagggatatg tatgtggatc aagagttgga tatcaacagg ctctctgatt acgatgttga
    10621 tcatatcgtg ccacagtcat tcttgaagga tgattctatc gataacaagg tgctcaccag
    10681 gtctgataag aacaggggta agagtgataa cgtgccaagt gaagaggttg tgaagaaaat
    10741 gaagaactat tggaggcagc tcctcaacgc taagctcatc actcagagaa agttcgataa
    10801 cttgactaag gctgagaggg gaggactctc tgaattggat aaggcaggat tcatcaagag
    10861 gcagcttgtg gaaaccaggc agatcactaa gcacgttgca cagatcctcg attctaggat
    10921 gaacaccaag tacgatgaga acgataagtt gatcagggaa gtgaaggtta tcaccctcaa
    10981 gtcaaagctc gtgtctgatt tcagaaagga tttccaattc tacaaggtga gggaaatcaa
    11041 caactaccac cacgctcacg atgcttacct taacgctgtt gttggaaccg ctctcatcaa
    11101 gaagtatcct aagctcgagt cagagttcgt gtacggtgat tacaaggtgt acgatgtgag
    11161 gaagatgatc gctaagtctg agcaagagat cggaaaggct accgctaagt atttcttcta
    11221 ctctaacatc atgaatttct tcaagaccga gattaccctc gctaacggtg agatcagaaa
    11281 gaggccactc atcgagacaa acggtgaaac aggtgagatc gtgtgggata agggaaggga
    11341 tttcgctacc gttagaaagg tgctctctat gccacaggtg aacatcgtta agaaaaccga
    11401 ggtgcagacc ggtggattct ctaaagagtc tatcctccct aagaggaact ctgataagct
    11461 cattgctagg aagaaggatt gggaccctaa gaaatacggt ggtttcgatt ctcctaccgt
    11521 ggcttactct gttctcgttg tggctaaggt tgagaaggga aagagtaaga agctcaagtc
    11581 tgttaaggaa cttctcggaa tcactatcat ggaaaggtca tctttcgaga agaacccaat
    11641 cgatttcctc gaggctaagg gatacaaaga ggttaagaag gatctcatca tcaagctccc
    11701 aaagtactca ctcttcgaac tcgagaacgg tagaaagagg atgctcgctt ctgctggtga
    11761 gcttcaaaag ggaaacgagc ttgctctccc atctaagtac gttaactttc tttacctcgc
    11821 ttctcactac gagaagttga agggatctcc agaagataac gagcagaagc aacttttcgt
    11881 tgagcagcac aagcactact tggatgagat catcgagcag atctctgagt tctctaaaag
    11941 ggtgatcctc gctgatgcaa acctcgataa ggtgttgtct gcttacaaca agcacagaga
    12001 taagcctatc agggaacagg cagagaacat catccatctc ttcaccctta ccaacctcgg
    12061 tgctcctgct gctttcaagt acttcgatac aaccatcgat aggaagagat acacctctac
    12121 caaagaagtg ctcgatgcta ccctcatcca tcagtctatc actggactct acgagactag
    12181 gatcgatctc tcacagctcg gtggtgattc aagggctgat cctaagaaga agaggaaggt
    12241 ttgaggcgcg ccgagctcca ggcctcccag ctttcgtccg tatcatcggt ttcgacaacg
    12301 ttcgtcaagt tcaatgcatc agtttcattg cccacacacc agaatcctac taagtttgag
    12361 tattatggca ttggaaaagc tgttttcttc tatcatttgt tctgcttgta atttactgtg
    12421 ttctttcagt ttttgttttc ggacatcaaa atgcaaatgg atggataaga gttaataaat
    12481 gatatggtcc ttttgttcat tctcaaatta ttattatctg ttgtttttac tttaatgggt
    12541 tgaatttaag taagaaagga actaacagtg tgatattaag gtgcaatgtt agacatataa
    12601 aacagtcttt cacctctctt tggttatgtc ttgaattggt ttgtttcttc acttatctgt
    12661 gtaatcaagt ttactatgag tctatgatca agtaattatg caatcaagtt aagtacagta
    12721 taggcttgag ctccctaggc ctgttatccc taacaagttt gtacaaaaaa gctgaacgag
    12781 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
    12841 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg
    12901 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag
    12961 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
    13021 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
    13081 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
    13141 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
    13201 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
    13261 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
    13321 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
    13381 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
    13441 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
    13501 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
    13561 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
    13621 cgatgagtgg caggcggggc gtaatctaga ggatccggct tactaaaagc cagataacag
    13681 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga
    13741 agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca
    13801 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat
    13861 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat
    13921 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg
    13981 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
    14041 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg
    14101 cacgtctgct gtcagataaa gtcccccgtg aactttaccc ggtggtgcat atcggggatg
    14161 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
    14221 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
    14281 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac
    14341 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
    14401 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttcga
    14461 taattccgat ccagcctagg cccgggcctg aggacgcgtc catggttaat taagacgtcc
    14521 ggaccgacta gtggatcctc tagagtcgac ctgcaggcat gcaagcttct tcaagtgtat
    14581 gtaggtatag taacatgaac aagaacagac tcaagtacaa gatcgcatac gaaaatggaa
    14641 atggcaatgt cacttccaca taatcaaaca cgaatcctca tatcaacaag gcctgagatt
    14701 ctaactagct cataacaact tagccaatag ttacttgaga ctaccaaatg tatgtagaac
    14761 taaagactaa gggacagaga gttcgtctaa acaggtgaat ctagtcgttg ttatctaata
    14821 aacaattcag ccccaaatgc agaacacaca tagagctctc tattgattca aattacgatc
    14881 tgatactgat aacgtctaga tttttagggt taaagcaatc aatcacctga cgattcaagg
    14941 tggttggatc atgacgattc cagaaaacat caagcaagct ctcaaagcta cactctttgg
    15001 gatcatactg aactctaaca acctcgttat gtcccgtagt gccagtacag acatcctcgt
    15061 aactcggatt gtgcacgatg ccatggctat acccaacctc ggtcttggtc acaccaggaa
    15121 ctctctggta agctagctcc actccccaga aacaaccggc gccaaattgc gcgaattgct
    15181 gacctgaaga cggaacatca tcgtcgggtc cttgggcgat tgcggcggaa gatgggtcag
    15241 cttgggcttg aggacgagac ccgaatccga gtctgttgaa aaggttgttc attggggatt
    15301 tgtatacgga gattggtcgt cgagaggttt gagggaaagg acaaatgggt ttggctctgg
    15361 agaaagagag tgcggcttta gagagagaat tgagaggttt agagagagat gcggcggcga
    15421 tgagcggagg agagacgacg aggacctgca ttatcaaagc agtgacgtgg tgaaatttgg
    15481 aacttttaag aggcagatag atttattatt tgtatccatt ttcttcattg ttctagaatg
    15541 tcgcggaaca aattttaaaa ctaaatccta aatttttcta attttgttgc caatagtgga
    15601 tatgtgggcc gtatagaagg aatctattga aggcccaaac ccatactgac gagcccaaag
    15661 gttcgttttg cgttttatgt ttcggttcga tgccaacgcc acattctgag ctaggcaaaa
    15721 aacaaacgtg tctttgaata gactcctctc gttaacacat gcagcggctg catggtgacg
    15781 ccattaacac gtggcctaca attgcatgat gtctccattg acacgtgact tctcgtctcc
    15841 tttcttaata tatctaacaa acactcctac ctcttccaaa atatatacac atctttttga
    15901 tcaatctctc attcaaaatc tcattctctc tagtaaacaa gaacaaaaaa atggcggata
    15961 cagctagagg aacccatcac gatatcatcg gcagagacca gtacccgatg atgggccgag
    16021 accgagacca gtaccagatg tccggacgag gatctgacta ctccaagtct aggcagattg
    16081 ctaaagctgc aactgctgtc acagctggtg gttccctcct tgttctctcc agccttaccc
    16141 ttgttggaac tgtcatagct ttgactgttg caacacctct gctcgttatc ttcagcccaa
    16201 tccttgtccc ggctctcatc acagttgcac tcctcatcac cggttttctt tcctctggag
    16261 ggtttggcat tgccgctata accgttttct cttggattta caagtaagca cacatttatc
    16321 atcttacttc ataattttgt gcaatatgtg catgcatgtg ttgagccagt agctttggat
    16381 caattttttt ggtcgaataa caaatgtaac aataagaaat tgcaaattct agggaacatt
    16441 tggttaacta aatacgaaat ttgacctagc tagcttgaat gtgtctgtgt atatcatcta
    16501 tataggtaaa atgcttggta tgatacctat tgattgtgaa taggtacgca acgggagagc
    16561 acccacaggg atcagacaag ttggacagtg caaggatgaa gttgggaagc aaagctcagg
    16621 atctgaaaga cagagctcag tactacggac agcaacatac tggtggggaa catgaccgtg
    16681 accgtactcg tggtggccag cacactacta tgagcgagct gattaaggag aacatgcaca
    16741 tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca tccgagggcg
    16801 aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc
    16861 tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaga accttcatca
    16921 accacaccca gggcatcccc gacttcttta agcagtcctt ccctgagggc ttcacatggg
    16981 agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc
    17041 aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttccca tccaacggcc
    17101 ctgtgatgca gaagaaaaca ctcggctggg aggccaacac cgagatgctg taccccgctg
    17161 acggcggcct ggaaggcaga agcgacatgg ccctgaagct cgtgggcggg ggccacctga
    17221 tctgcaactt caagaccaca tacagatcca agaaacccgc taagaacctc aagatgcccg
    17281 gcgtctacta tgtggaccac agactggaaa gaatcaagga ggccgacaaa gagacctacg
    17341 tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca
    17401 agtgatctag taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata
    17461 ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc
    17521 tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa
    17581 attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc
    17641 aaatgtttga acgataagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
    17701 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
    17761 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
    17821 agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac
    17881 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa cggatattta
    17941 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt
    18001 cccctcggga tcaa
//
