LOCUS       Exported               17497 bp ds-DNA     circular SYN 25-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17497)
  AUTHORS   Rob
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 25, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17497
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      2541..3541
                     /label=pVS-1 ori
                     /note="pVS-1 ori"
     rep_origin      4247..4835
                     /label=pUC19 ori
                     /note="pUC19 ori"
     CDS             complement(5079..5870)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_signal     6398..6422
                     /label=LB
                     /note="LB"
     primer_bind     6697..6713
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6728..7699
                     /label=PcUbi(P)
                     /note="PcUbi(P)"
     CDS             7714..7734
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7759..11676
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     CDS             11677..11724
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    11742..12210
                     /label=Pea3A(T)
                     /note="Pea3A(T)"
     protein_bind    12236..12360
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             12469..13149
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             13469..13774
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(13815..13939)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        14052..15433
                     /label=pOLE1 promoter
                     /note="OLE1prom (-1382)"
                     /note="As in pEDO97 (FastRed cassette)"
     misc_feature    15434..16192
                     /label=OLE1 gene
                     /note="OLE1genome"
                     /note="As in pEDO97 (FastRed cassette)"
     exon            16199..16888
                     /label=tagRFP
                     /note="tagRFP"
                     /note="As in pEDO97 (FastRed cassette)"
     misc_feature    16889..17138
                     /label=NOST
                     /note="NOST"
     primer_bind     complement(17148..17164)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_signal     17360..17384
                     /label=RB
                     /note="RB"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
     2281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     2341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     2401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     2461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     2521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     2581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     2641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     2701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     2761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     2821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     2881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     2941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     3001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     3061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     3121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     3181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     3241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     3301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     3361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     3421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     3481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     3541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     3601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     3661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     3721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     3781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     3901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     3961 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     4021 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     4081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat
     4921 ctcccaattt gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct
     4981 gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta atcgcttgag ttaacgccgg
     5041 cgaagcggcg tcggcttgaa cgaatttcta gctagacatt atttgccgac taccttggtg
     5101 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
     5161 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
     5221 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
     5281 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg
     5341 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
     5401 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
     5461 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
     5521 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
     5581 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
     5641 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
     5701 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
     5761 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
     5821 actacctctg atagttgagt cgatacttcg gcgatcaccg cttcccccat gatgtttaac
     5881 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
     5941 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa
     6001 aaacatgtca taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     6061 ggtcaaggtt ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga
     6121 accgaacgag gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc
     6181 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
     6241 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
     6301 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
     6361 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
     6421 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
     6481 acgccgaatt gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6541 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6601 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6661 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
     6721 cgaattcaaa aattacggat atgaatatag gcatatccgt atccgaatta tccgtttgac
     6781 agctagcaac gattgtacaa ttgcttcttt aaaaaaggaa gaaagaaaga aagaaaagaa
     6841 tcaacatcag cgttaacaaa cggccccgtt acggcccaaa cggtcatata gagtaacggc
     6901 gttaagcgtt gaaagactcc tatcgaaata cgtaaccgca aacgtgtcat agtcagatcc
     6961 cctcttcctt caccgcctca aacacaaaaa taatcttcta cagcctatat atacaacccc
     7021 cccttctatc tctcctttct cacaattcat catctttctt tctctacccc caattttaag
     7081 aaatcctctc ttctcctctt cattttcaag gtaaatctct ctctctctct ctctctctgt
     7141 tattccttgt tttaattagg tatgtattat tgctagtttg ttaatctgct tatcttatgt
     7201 atgccttatg tgaatatctt tatcttgttc atctcatccg tttagaagct ataaatttgt
     7261 tgatttgact gtgtatctac acgtggttat gtttatatct aatcagatat gaatttcttc
     7321 atattgttgc gtttgtgtgt accaatccga aatcgttgat ttttttcatt taatcgtgta
     7381 gctaattgta cgtatacata tggatctacg tatcaattgt tcatctgttt gtgtttgtat
     7441 gtatacagat ctgaaaacat cacttctctc atctgattgt gttgttacat acatagatat
     7501 agatctgtta tatcattttt tttattaatt gtgtatatat atatgtgcat agatctggat
     7561 tacatgattg tgattattta catgattttg ttatttacgt atgtatatat gtagatctgg
     7621 actttttgga gttgttgact tgattgtatt tgtgtgtgta tatgtgtgtt ctgatcttga
     7681 tatgttatgt atgtgcagcg aattcggcgc gcccctaaga agaagcggaa ggttggtatt
     7741 cacggggtgc ctgcggctac gcagttcgag gggttcacca acctctacca ggtcagcaag
     7801 acgctgcggt tcgagctgat tccgcagggc aagaccctca agcacatcca ggagcagggc
     7861 tttatcgagg aggacaaagc gcggaacgac cactacaagg agctcaagcc gatcatcgac
     7921 cggatctaca agacctacgc ggaccagtgc ctgcagctcg tgcagctcga ctgggagaac
     7981 ctctcggccg ccatcgactc ctaccgcaag gagaagacgg aggagacacg caacgcgctc
     8041 atcgaagaac aggctacgta tcgcaacgct atccacgact acttcatcgg gcgcacagat
     8101 aatctcaccg acgccatcaa caagcgccac gccgaaatct ataaggggct gtttaaggcc
     8161 gagctgttca atggcaaagt gctgaagcaa ctgggcaccg tcacgaccac agagcatgag
     8221 aacgccctcc tccggtcgtt cgacaagttt acgacatact ttagcggctt ttacgagaac
     8281 cgcaaaaacg tgttcagcgc cgaggacatt agcaccgcca tcccgcatag gatcgtgcaa
     8341 gacaacttcc cgaagttcaa ggagaactgc cacatcttca cccgcctcat cacggccgtg
     8401 ccgtccctcc gcgagcactt cgagaacgtg aagaaggcca tcgggatctt cgtgtccacc
     8461 tcgatcgaag aggtgttctc cttccccttc tacaatcagc tgctgacgca gacccagatc
     8521 gacctctaca accagctcct cggcggcatc tcccgcgagg ccggcaccga gaagatcaag
     8581 ggcctgaacg aggtgctcaa tctcgccatc cagaagaacg acgaaaccgc gcacatcatt
     8641 gcctcactcc cacataggtt tatccccctg tttaagcaga tcctctccga ccgcaacacg
     8701 ttgtccttca tcctcgagga gttcaagagc gacgaggagg tcatccagtc cttctgcaag
     8761 tacaagaccc tcctccgcaa cgagaatgtg ctcgaaaccg ccgaggcgct gttcaatgag
     8821 ctcaatagca tcgatctcac ccacatcttc atctcccata agaagctgga aaccatctcc
     8881 agcgccctgt gcgaccactg ggacaccctc cgcaacgccc tctacgagcg gcgcatcagc
     8941 gagctcaccg gcaagatcac gaagtcggcg aaagagaaag tgcaaaggag cctcaagcac
     9001 gaggacatta acctgcagga gatcatctcc gccgcgggca aggagctgtc cgaggctttc
     9061 aagcagaaga cctcggagat cctctctcac gcccacgcgg ccctggacca gccgctgccg
     9121 acgaccctga aaaagcaaga ggaaaaggaa atcctcaagt cccagctgga cagcctcctg
     9181 gggctgtacc accttctcga ctggttcgcc gtggacgagt ccaacgaggt cgatccggag
     9241 tttagcgccc gcctcaccgg gatcaagctt gagatggaac ctagcttgag cttctataat
     9301 aaggcgcgca attacgcgac caagaagccg tattccgtgg agaagttcaa gctgaacttc
     9361 caaatgccca ccctggccag cgggtgggac gttaacaaag agaagaacaa cggagccatt
     9421 cttttcgtga aaaatgggtt gtattatttg ggaattatgc cgaaacaaaa aggcaggtac
     9481 aaggcgctca gcttcgagcc aactgagaag acctccgagg gcttcgacaa gatgtactac
     9541 gattattttc ctgacgctgc aaagatgata ccgaagtgca gcactcagct taaggcggtg
     9601 acggcgcact ttcagaccca taccaccccc atcctcctct ccaacaactt catcgagccg
     9661 ctcgagatca ccaaggagat atacgatctg aataatccag aaaaggaacc caagaagttc
     9721 cagaccgcct acgccaagaa gacgggcgat caaaaggggt atagagaggc gctctgcaag
     9781 tggatcgact tcacgcgcga tttcctcagc aagtacacca agacaacctc catcgatctc
     9841 tcttccctcc gcccctcttc ccagtacaag gacctcgggg agtactacgc cgaactcaac
     9901 ccactcctgt atcacatctc gtttcagcgt atcgcggaaa aggagatcat ggacgccgtc
     9961 gaaaccggca agttgtatct tttccaaatc tataacaagg acttcgcgaa gggccaccac
    10021 gggaagccaa acctgcacac cctctactgg acaggcctct tcagcccgga aaatctcgcg
    10081 aagacgagca taaagctgaa cggccaggca gaactcttct acaggccgaa gtccaggatg
    10141 aagcgcatgg ctcatcgcct cggtgagaag atgctgaaca agaagctgaa agatcaaaag
    10201 acgccaatcc ctgatacact gtatcaggag ctgtacgatt acgtgaacca ccgcctctca
    10261 cacgacctca gcgacgaggc ccgcgcgctc ctgccaaacg tcatcacgaa ggaggtcagc
    10321 cacgagatca taaaggatcg gcggtttacc tctgacaagt tctttttcca tgtccccatc
    10381 acgctgaact accaggccgc gaatagcccg tccaagttca accagcgggt caacgcgtat
    10441 ctcaaggagc acccagagac acccataatc gggattgacc ggggggagcg gaacctcatc
    10501 tacatcaccg tcatcgactc caccggaaag attctcgagc aacggtcgct caataccatc
    10561 cagcagttcg actaccagaa gaagctcgac aaccgggaga aggaacgcgt cgccgcgagg
    10621 caggcctggt ccgtagtggg cacgatcaaa gacctgaagc agggctatct cagccaggtc
    10681 atccatgaga tagtggatct catgatccac taccaagccg tcgtggtcct cgagaatctc
    10741 aatttcggat tcaaatccaa gcgcacaggc atcgccgaga aggcggtgta ccaacagttc
    10801 gagaaaatgc ttatcgacaa gctcaattgc ctggtgctca aggactatcc ggcggagaag
    10861 gtcggggggg tcctcaatcc gtatcagctg accgaccagt ttacgtcatt tgcgaagatg
    10921 ggcacccaga gcggcttcct tttctatgtc ccggccccat atacctcaaa gattgatccc
    10981 ttgaccggat tcgtggaccc gtttgtctgg aagaccatca agaaccatga gtcgcgtaag
    11041 catttcctgg agggtttcga cttcctgcac tatgatgtaa aaaccggaga cttcatcctg
    11101 catttcaaga tgaatcggaa cctctccttc cagcggggac tccctggctt catgcccgct
    11161 tgggatatcg tttttgagaa aaatgaaacc caattcgacg ccaaaggcac gcctttcatc
    11221 gcgggcaaga ggattgtccc tgtaattgag aaccatagat tcaccgggcg ttaccgtgac
    11281 ctgtaccccg caaacgaact catcgccctc ctggaggaga aaggcatcgt tttccgcgac
    11341 gggtcaaata tcctccccaa actgctcgag aacgatgata gccacgctat tgacacgatg
    11401 gtagcgctca tcagatccgt gctgcaaatg agaaattcaa atgctgccac tggggaggat
    11461 tacatcaact cccctgtgcg tgatctcaat ggcgtgtgct tcgattctag atttcagaat
    11521 cctgagtggc cgatggatgc cgatgctaac ggcgcatacc acatagcatt gaaaggacaa
    11581 ctgttgttga accatctcaa ggagagcaag gaccttaagc tgcagaacgg catcagcaac
    11641 caggattggc ttgcctatat ccaagagctc cgcaataagc gtcctgctgc caccaaaaag
    11701 gccggacagg ctaagaaaaa gaagtgaggc gcgccgagct ccaggcctcc cagctttcgt
    11761 ccgtatcatc ggtttcgaca acgttcgtca agttcaatgc atcagtttca ttgcccacac
    11821 accagaatcc tactaagttt gagtattatg gcattggaaa agctgttttc ttctatcatt
    11881 tgttctgctt gtaatttact gtgttctttc agtttttgtt ttcggacatc aaaatgcaaa
    11941 tggatggata agagttaata aatgatatgg tccttttgtt cattctcaaa ttattattat
    12001 ctgttgtttt tactttaatg ggttgaattt aagtaagaaa ggaactaaca gtgtgatatt
    12061 aaggtgcaat gttagacata taaaacagtc tttcacctct ctttggttat gtcttgaatt
    12121 ggtttgtttc ttcacttatc tgtgtaatca agtttactat gagtctatga tcaagtaatt
    12181 atgcaatcaa gttaagtaca gtataggctt gagctcccta ggcctgttat ccctaacaag
    12241 tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta
    12301 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg
    12361 gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg
    12421 attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
    12481 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
    12541 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
    12601 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
    12661 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
    12721 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
    12781 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
    12841 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
    12901 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
    12961 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
    13021 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
    13081 cttaatgaat tacaacagta ctgcgatgag tggcaggcgg ggcgtaatct agaggatccg
    13141 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa
    13201 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat
    13261 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc
    13321 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg
    13381 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct
    13441 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga
    13501 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg
    13561 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtccccc gtgaacttta
    13621 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt
    13681 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa
    13741 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca
    13801 gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
    13861 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
    13921 ctttcttgta caaagtggtt cgataattcc gatccagcct aggcccgggc ctgaggacgc
    13981 gtccatggtt aattaagacg tccggaccga ctagtggatc ctctagagtc gacctgcagg
    14041 catgcaagct tcttcaagtg tatgtaggta tagtaacatg aacaagaaca gactcaagta
    14101 caagatcgca tacgaaaatg gaaatggcaa tgtcacttcc acataatcaa acacgaatcc
    14161 tcatatcaac aaggcctgag attctaacta gctcataaca acttagccaa tagttacttg
    14221 agactaccaa atgtatgtag aactaaagac taagggacag agagttcgtc taaacaggtg
    14281 aatctagtcg ttgttatcta ataaacaatt cagccccaaa tgcagaacac acatagagct
    14341 ctctattgat tcaaattacg atctgatact gataacgtct agatttttag ggttaaagca
    14401 atcaatcacc tgacgattca aggtggttgg atcatgacga ttccagaaaa catcaagcaa
    14461 gctctcaaag ctacactctt tgggatcata ctgaactcta acaacctcgt tatgtcccgt
    14521 agtgccagta cagacatcct cgtaactcgg attgtgcacg atgccatggc tatacccaac
    14581 ctcggtcttg gtcacaccag gaactctctg gtaagctagc tccactcccc agaaacaacc
    14641 ggcgccaaat tgcgcgaatt gctgacctga agacggaaca tcatcgtcgg gtccttgggc
    14701 gattgcggcg gaagatgggt cagcttgggc ttgaggacga gacccgaatc cgagtctgtt
    14761 gaaaaggttg ttcattgggg atttgtatac ggagattggt cgtcgagagg tttgagggaa
    14821 aggacaaatg ggtttggctc tggagaaaga gagtgcggct ttagagagag aattgagagg
    14881 tttagagaga gatgcggcgg cgatgagcgg aggagagacg acgaggacct gcattatcaa
    14941 agcagtgacg tggtgaaatt tggaactttt aagaggcaga tagatttatt atttgtatcc
    15001 attttcttca ttgttctaga atgtcgcgga acaaatttta aaactaaatc ctaaattttt
    15061 ctaattttgt tgccaatagt ggatatgtgg gccgtataga aggaatctat tgaaggccca
    15121 aacccatact gacgagccca aaggttcgtt ttgcgtttta tgtttcggtt cgatgccaac
    15181 gccacattct gagctaggca aaaaacaaac gtgtctttga atagactcct ctcgttaaca
    15241 catgcagcgg ctgcatggtg acgccattaa cacgtggcct acaattgcat gatgtctcca
    15301 ttgacacgtg acttctcgtc tcctttctta atatatctaa caaacactcc tacctcttcc
    15361 aaaatatata cacatctttt tgatcaatct ctcattcaaa atctcattct ctctagtaaa
    15421 caagaacaaa aaaatggcgg atacagctag aggaacccat cacgatatca tcggcagaga
    15481 ccagtacccg atgatgggcc gagaccgaga ccagtaccag atgtccggac gaggatctga
    15541 ctactccaag tctaggcaga ttgctaaagc tgcaactgct gtcacagctg gtggttccct
    15601 ccttgttctc tccagcctta cccttgttgg aactgtcata gctttgactg ttgcaacacc
    15661 tctgctcgtt atcttcagcc caatccttgt cccggctctc atcacagttg cactcctcat
    15721 caccggtttt ctttcctctg gagggtttgg cattgccgct ataaccgttt tctcttggat
    15781 ttacaagtaa gcacacattt atcatcttac ttcataattt tgtgcaatat gtgcatgcat
    15841 gtgttgagcc agtagctttg gatcaatttt tttggtcgaa taacaaatgt aacaataaga
    15901 aattgcaaat tctagggaac atttggttaa ctaaatacga aatttgacct agctagcttg
    15961 aatgtgtctg tgtatatcat ctatataggt aaaatgcttg gtatgatacc tattgattgt
    16021 gaataggtac gcaacgggag agcacccaca gggatcagac aagttggaca gtgcaaggat
    16081 gaagttggga agcaaagctc aggatctgaa agacagagct cagtactacg gacagcaaca
    16141 tactggtggg gaacatgacc gtgaccgtac tcgtggtggc cagcacacta ctatgagcga
    16201 gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtga acaaccacca
    16261 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat
    16321 caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ccagcttcat
    16381 gtacggcagc agaaccttca tcaaccacac ccagggcatc cccgacttct ttaagcagtc
    16441 cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac
    16501 cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg
    16561 ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggccaa
    16621 caccgagatg ctgtaccccg ctgacggcgg cctggaaggc agaagcgaca tggccctgaa
    16681 gctcgtgggc gggggccacc tgatctgcaa cttcaagacc acatacagat ccaagaaacc
    16741 cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac cacagactgg aaagaatcaa
    16801 ggaggccgac aaagagacct acgtcgagca gcacgaggtg gctgtggcca gatactgcga
    16861 cctccctagc aaactggggc acaagtgatc tagtaacata gatgacaccg cgcgcgataa
    16921 tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg
    16981 gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac
    17041 atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt
    17101 caatcttaag aaactttatt gccaaatgtt tgaacgataa gcttggcact ggccgtcgtt
    17161 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
    17221 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
    17281 ttgcgcagcc tgaatggcga atgagcttga gcttggatca gattgtcgtt tcccgccttc
    17341 agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg
    17401 tttattagaa taacggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
    17461 atgtgcatgc caaccacagg gttcccctcg ggatcaa
//
