LOCUS       Exported               10556 bp ds-DNA     circular SYN 26-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10556)
  AUTHORS   UMC-Utrecht
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 26, 2021 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10556
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    8..10
                     /label=stop
     misc_feature    14..16
                     /label=stop
     misc_feature    19..21
                     /label=stop
     misc_feature    24..26
                     /label=stop
     CDS             44..644
                     /label=IRES
     primer_bind     complement(196..215)
                     /label=BP128 IRES Seq 160 Rev
     primer_bind     196..215
                     /label=BP127 IRES Seq 160 Fw
     primer_bind     204..219
                     /label=Primer 15
     primer_bind     504..522
                     /label=Primer 16
     primer_bind     524..543
                     /label=BP129 IRES Seq 490 Fw
     primer_bind     622..697
                     /label=GK231 IRES-Blas-Gib-F
     primer_bind     complement(622..654)
                     /label=GK233 IRES-Neo-Gib-F
     CDS             652..1050
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=blast
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(1020..1107)
                     /label=GK232 Blas-pLV-Gib-R
     primer_bind     complement(1168..1186)
                     /label=KO rv-pLV_Puro/end-seq.primer #19
     LTR             1896..2129
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2201..2322
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2235..2258)
                     /label=BP190 Seq Rev into TagGFP of Casper3
     rep_origin      2341..2476
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(2509..2527)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2537..2553)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2698..3153
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3182..3286
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3287..4147
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4113..4132
                     /label=BP 048 Amp_HA2_control_fw
     rep_origin      4318..4906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5194..5215
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     5214..5233
                     /label=BP258 Seq pr in miniTol vector (T2) Rev (2)
     primer_bind     5214..5233
                     /label=BP258 Seq pr in miniTol vector (T2) Rev
     promoter        5230..5260
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5268..5284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5292..5308
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5329..5347
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             5631..5811
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5855..5980
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     primer_bind     5999..6018
                     /label=BP 001 seq TGF-RE Fw1
     primer_bind     6212..6232
                     /label=BP 002 seq TGF-RE Fw2
     primer_bind     6253..6275
                     /label=BP 003 seq TGF-RE Fw3
     misc_feature    6473..6706
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     primer_bind     6925..6944
                     /label=BP 022 seq TGF-RE Fw4
     primer_bind     7052..7071
                     /label=BP 023 seq TGF-RE Fw5
     primer_bind     complement(7068..7087)
                     /label=KO rv-pLV-seq.primer #38
     primer_bind     7112..7131
                     /label=BP 024 seq TGF-RE Fw6
     primer_bind     7154..7172
                     /label=KO fw-pLV_TOP5-seq.primer #16
     primer_bind     7173..7192
                     /label=BP 025 seq TGF-RE Fw7
     misc_feature    7196..7313
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7361..8523
                     /label=hEF1 promoter
     primer_bind     complement(7417..7447)
                     /label=GK228 pLV-IRES-Gib-F
     primer_bind     8451..8470
                     /label=KO fw-hEf1a(end) #88
     misc_feature    8596..8601
                     /label=EcoRI
     misc_feature    8605..9315
                     /label=YPet
     misc_feature    9322..9486
                     /label=WW domain
     misc_feature    9499..9714
                     /label=EV linker
     misc_feature    9499..9510
                     /label=Feature 23
     misc_feature    9511..9522
                     /label=Feature 24
     misc_feature    9523..9528
                     /label=Feature 25
     misc_feature    9529..9540
                     /label=Feature 43
     misc_feature    9541..9552
                     /label=Feature 32
     misc_feature    9553..9570
                     /label=Feature 46
     misc_feature    9571..9582
                     /label=Feature 33
     misc_feature    9583..9594
                     /label=Feature 34
     misc_feature    9595..9606
                     /label=Feature 35
     misc_feature    9607..9618
                     /label=Feature 36
     misc_feature    9619..9636
                     /label=Feature 44
     misc_feature    9637..9648
                     /label=Feature 37
     misc_feature    9649..9666
                     /label=Feature 47
     misc_feature    9667..9678
                     /label=Feature 38
     misc_feature    9679..9690
                     /label=Feature 39
     misc_feature    9691..9702
                     /label=Feature 40
     misc_feature    9703..9714
                     /label=Feature 41
     misc_feature    9727..9780
                     /label=ERK Substrate
     misc_feature    9742..9744
                     /label=fosf.site
     CDS             9754..9756
                     /label=P
     CDS             9760..9762
                     /label=W
     CDS             9790..10500
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)Mutation K206A = monomeric to sticky
                     Mutation V224L = enhances dimerization sysynergistically 
                     with S208F of YPet (Vinkenborg. J.L. et al., 2007)
                     "
                     /label=Turquoise2 FRET-optimized
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFLTAAGITLGMDEL"
     variation       9985..9987
                     /label=T65S , mTurq
     variation       9988..9990
                     /label=Y66W
     variation       10006..10008
                     /label=S72A
     variation       10177..10179
                     /label=G129D
     misc_feature    10228..10230
                     /label=I146F , mTurq2
     variation       10249..10251
                     /label=M153T
     variation       10279..10281
                     /label=V163A
     variation       10315..10317
                     /label=S175G
     misc_feature    10408..10410
                     /label=K206A monomeric->sticky
     variation       10462..10464
                     /label=V224L enhances dimerization
     CDS             10507..10527
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     join(10550..10556,1..67)
                     /label=GK228 pLV-IRES-Gib-F
ORIGIN
        1 cggtcgttaa cattaagcta gcctagtgcc atttgttcag tggcccggga attccgcccc
       61 tctccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
      121 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
      181 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
      241 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
      301 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
      361 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
      421 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
      481 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
      541 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
      601 tcctttgaaa aacacgatga taatatggat atggccacaa cggtcgccac catggccaag
      661 cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc
      721 cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc
      781 actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc
      841 actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac
      901 aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg
      961 atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg
     1021 ctgccctctg gttatgtgtg ggagggctaa tacgtaatgc atgtattcaa tctaagcagg
     1081 ctttcacttt ctcgccaact tacaaggcct ttctgtgtaa acaatacctg aacctttacc
     1141 ccgttgcccg gcaacggcca ggtctgtgcc aagtgtttgc tgacgcaacc cccactggct
     1201 ggggcttggt catgggccat cagcgcatgc gtggaacctt ttcggctcct ctgccgatcc
     1261 atactgcgga actcctagcc gcttgttttg ctcgcagcag gtctggagca aacattatcg
     1321 ggactgataa ctctgttgtc ctatcccgca aatatacatc gtttccatgg ctgctaggct
     1381 gtgctgccaa ctggatcctg cgcgggacgt cctttgttta cgtcccgtcg gcgctgaatc
     1441 ctgcggacga cccttctcgg ggtcgcttgg gactctctcg tccccttctc cgtctgccgt
     1501 tccgaccgac cacggggcgc acctctcttt acgcggactc cccgtctgtg ccttctcatc
     1561 tgccggaccg tgtgcacttc gcttcacctc tgcacgtcgc atggagacca ccgtgaacgc
     1621 ccaccaaata ttgcccaagg tcttacataa gaggactctt ggactctcag caatgtcaac
     1681 gaccgacctt gaggcatact tcaaagactg tttgtttaaa gactgggagg agttggggga
     1741 ggagattagg ttaaaggtct ttgtactagg aggctgtagg cataaattgg tctgcgcacc
     1801 agcaccatgt atcactagag cggggtacct ttaagaccaa tgacttacaa ggcagctgta
     1861 gatcttagcc actttttaaa agaaaagggg ggacttggaa gggctaattc actcccaacg
     1921 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     1981 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     2041 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     2101 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     2161 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     2221 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2281 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     2341 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     2401 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     2461 ggcttttttg gaggcctagg cttttgcgtc gagacgtacc caattcgccc tatagtgagt
     2521 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     2581 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     2641 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg
     2701 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     2761 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     2821 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     2881 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     2941 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     3001 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     3061 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     3121 cgaattttaa caaaatatta acgtttacaa tttcccaggt ggcacttttc ggggaaatgt
     3181 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3241 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     3301 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3361 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3421 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3481 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     3541 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     3601 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     3661 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     3721 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3781 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     3841 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     3901 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     3961 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     4021 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4081 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     4141 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4201 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4261 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4321 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4381 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4441 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     4501 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4561 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4621 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4681 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4741 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4801 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4861 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4921 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4981 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     5041 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     5101 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     5161 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     5221 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     5281 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     5341 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     5401 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     5461 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     5521 ggtctgacat ggattggacg aaccactgaa ttggaggcgt ggcctgggcg ggactgggga
     5581 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     5641 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     5701 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5761 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     5821 gaacagggac ctgaaagcga aagggaaacc agagctctct cgacgcagga ctcggcttgc
     5881 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     5941 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     6001 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     6061 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     6121 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     6181 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     6241 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     6301 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     6361 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     6421 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     6481 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcctca atgacgctga
     6541 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     6601 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     6661 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     6721 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     6781 ctctggaaca gattggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     6841 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     6901 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     6961 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     7021 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     7081 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     7141 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcggtttta
     7201 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     7261 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatcgatc
     7321 atatgactag agcggcctta cgacattgat tattgactag ggctccggtg cccgtcagtg
     7381 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     7441 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     7501 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     7561 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     7621 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctccagta
     7681 cgtgattctt gatcccgagc tggagccagg ggcgggcctt gcgctttagg agccccttcg
     7741 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
     7801 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
     7861 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca ggatctgcac
     7921 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
     7981 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
     8041 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
     8101 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
     8161 gctccagggg gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
     8221 acacaaagga aaggggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
     8281 cgggcgccgt ccaggcacct cgattagttc tggagctttt ggagtacgtc gtctttaggt
     8341 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     8401 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     8461 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg
     8521 tgattaatta aacgcgtacc gatagcttcg aattctgcag tcgacgatat cactagtctc
     8581 gagcattttg gcaaagaatt cggcatggtg agcaagggcg aggagctgtt caccggggtg
     8641 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     8701 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agcttctatg caccaccggc
     8761 aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct gcagtgcttc
     8821 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     8881 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     8941 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     9001 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     9061 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
     9121 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     9181 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgtt caaagacccc
     9241 aacgagaagc gcgatcacat ggtcctgctg gagttcctga ccgccgccgg gatcactgag
     9301 ggcatgaacg agctgtacct cgagatggcg gacgaggaga agctgccgcc cggctgggag
     9361 aagcgcatga gccgcagctc aggccgagtg tactacttca accacatcac taacgccagc
     9421 cagtgggagc ggcccagcgg caacagcagc agtggtggca aaaacgggca gggggagcct
     9481 gccaggggta ccagtgctgg tggtagtgct ggtggtagtg ctggtggttc cggcagtgct
     9541 ggtggtagtg ctggtggtag taccagtgct ggtggtagtg ctggtggtag tgctggtggt
     9601 agtgctggtg gtagtgctgg tggttccggc agtgctggtg gtagtgctgg tggtagtacc
     9661 agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg ctggtggtag tgctggtggt
     9721 tccggaccag atgtccctag aactccagtg gatccagcat ggctgtcatt ccaatttccg
     9781 ggcggccgca tggtgagtaa aggggaagaa ctgtttactg gcgtggtgcc aattctggtc
     9841 gaactggatg gagatgtaaa tgggcataaa tttagtgtgt ctggagaagg ggaaggagac
     9901 gctacatatg ggaaactgac actgaaattt atttgtacta ctggaaaact gcctgtgcct
     9961 tggcctacac tcgtgacaac cctgtcttgg ggagtgcagt gttttgcccg gtatcctgat
    10021 catatgaaac agcatgattt tttcaaatct gccatgcctg agggatatgt ccaggaacgg
    10081 acaattttct ttaaagatga tgggaattat aaaacacggg ccgaagtgaa atttgaaggg
    10141 gatacactgg tgaatcggat tgaactgaaa ggaattgatt ttaaagaaga tgggaatatt
    10201 ctgggccata aactggaata taattatttc agcgataatg tctacattac agccgataaa
    10261 cagaagaatg ggattaaagc caattttaaa attcggcata atattgaaga tggaggggtg
    10321 cagctcgccg atcattatca gcagaataca cctattgggg atgggcctgt gctgctgcct
    10381 gataatcatt atctgagcac acagtctgcc ctgagcaagg accctaatga aaagcgggac
    10441 catatggtcc tgctggaatt tctgactgcc gccggcatta cactcgggat ggatgaactg
    10501 tctagaccta agaagaagcg taaggtgtaa gtcgacggtc gagtcgagac gctagc
//
