LOCUS       Exported               10916 bp ds-DNA     circular SYN 26-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10916)
  AUTHORS   UMC-Utrecht
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 26, 2021 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10916
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..21
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(12..44)
                     /label=BP 220 EKAREVnls to pLV Rev
     primer_bind     complement(12..34)
                     /label=BP 222 EKAREVnls to E4 (or EF1a) Rev
     primer_bind     93..119
                     /label=BP654 Fw Make P60
     primer_bind     206..229
                     /label=GK228 pLV-IRES-Gib-F
     misc_feature    220..790
                     /label=IRES
     primer_bind     228..247
                     /label=BP612 Fw PCR1 (TR2)
     primer_bind     232..252
                     /label=BP440 P4 to Puro Fw
     primer_bind     complement(328..344)
                     /label=BP572 Rv  Prepare TV27
     primer_bind     complement(358..377)
                     /label=BP128 IRES Seq 160 Rev
     primer_bind     358..377
                     /label=BP127 IRES Seq 160 Fw
     primer_bind     366..381
                     /label=Primer 15
     primer_bind     666..684
                     /label=Primer 16
     primer_bind     686..705
                     /label=BP129 IRES Seq 490 Fw
     primer_bind     complement(756..785)
                     /label=BP398 Fragm2_Rev
     primer_bind     complement(774..821)
                     /label=GK230 IRES-Neo-Gib-R
     primer_bind     complement(774..820)
                     /label=GK229 IRES-Blas-Gib-R
     primer_bind     complement(775..807)
                     /label=BP613 Rv PCR1 (TR2)
     misc_feature    791..800
                     /label=IRES
     primer_bind     812..837
                     /label=BP095 Fusion PCR PGK-Puro 03 Fw
     primer_bind     812..829
                     /label=BP040 Puro Fw
     CDS             818..1417
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(947..965)
                     /label=JP030
     primer_bind     complement(953..972)
                     /label=BP123 Puro Seq Rev140
     primer_bind     955..973
                     /label=JP006
     primer_bind     955..973
                     /label=KO fw-Puro-seq.primer #37
     primer_bind     1217..1234
                     /label=BP121 Puro Seq Fw400
     primer_bind     complement(1217..1234)
                     /label=BP124 Puro Seq Rev410
     primer_bind     1314..1333
                     /label=BP122 Puro Seq Fw510
     primer_bind     complement(1401..1418)
                     /label=BP098 Fusion PCR PGK-Puro 06 Rev
     primer_bind     complement(1401..1418)
                     /label=BP041 Puro Rv
     primer_bind     complement(1401..1418)
                     /label=BP234 PGK-Puro into T4 Rev
     primer_bind     complement(1401..1417)
                     /label=BP096 Fusion PCR PGK-Puro 04 Rev
     primer_bind     complement(1401..1417)
                     /label=BP170 PGK:Puro w/o polyAis Rev
     primer_bind     complement(1401..1417)
                     /label=BP586  ires-Puro into E17 Rv
     primer_bind     complement(1401..1417)
                     /label=BP168 PGK:Puro w/o polyAis Rev
     primer_bind     complement(1401..1417)
                     /label=BP471 Make TV22 PCR2 Rv
     primer_bind     complement(1401..1417)
                     /label=BP629 Rv F28-CX
     primer_bind     1471..1486
                     /label=BP199 make E13&E14 with Infusion Sense
     primer_bind     complement(1578..1596)
                     /label=KO rv-pLV_Puro/end-seq.primer #19
     primer_bind     complement(1706..1725)
                     /label=BP476 Seq TV20 mini MCS
     LTR             2306..2539
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2611..2732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2645..2668)
                     /label=BP190 Seq Rev into TagGFP of Casper3
     primer_bind     2703..2729
                     /label=BP456 Insulator into T30 Fw
     primer_bind     2703..2729
                     /label=BP466 pUNKI-insulator into K4 Fw
     primer_bind     complement(2725..2742)
                     /label=BP499 polyA into TV22 Fw
     rep_origin      2751..2886
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(2919..2937)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2947..2963)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3108..3563
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3592..3696
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3697..4557
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4523..4542
                     /label=BP 048 Amp_HA2_control_fw
     rep_origin      4728..5316
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5604..5625
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     5624..5643
                     /label=BP258 Seq pr in miniTol vector (T2) Rev (2)
     primer_bind     5624..5643
                     /label=BP258 Seq pr in miniTol vector (T2) Rev
     promoter        5640..5670
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5678..5694
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5702..5718
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5739..5757
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             6041..6221
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6265..6390
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     primer_bind     6409..6428
                     /label=BP 001 seq TGF-RE Fw1
     primer_bind     6622..6642
                     /label=BP 002 seq TGF-RE Fw2
     primer_bind     6663..6685
                     /label=BP 003 seq TGF-RE Fw3
     misc_feature    6883..7116
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     primer_bind     7335..7354
                     /label=BP 022 seq TGF-RE Fw4
     primer_bind     7462..7481
                     /label=BP 023 seq TGF-RE Fw5
     primer_bind     complement(7478..7497)
                     /label=KO rv-pLV-seq.primer #38
     primer_bind     7522..7541
                     /label=BP 024 seq TGF-RE Fw6
     primer_bind     7564..7582
                     /label=KO fw-pLV_TOP5-seq.primer #16
     primer_bind     7583..7602
                     /label=BP 025 seq TGF-RE Fw7
     misc_feature    7606..7723
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7771..8933
                     /label=hEF1 promoter
     primer_bind     complement(7827..7857)
                     /label=GK228 pLV-IRES-Gib-F
     primer_bind     8842..8861
                     /label=BP308 seq vanaf EF1a
     primer_bind     8861..8880
                     /label=KO fw-hEf1a(end) #88
     primer_bind     complement(8916..8957)
                     /label=BP489 EF1a ipv CMV  Rv
     primer_bind     complement(8916..8933)
                     /label=BP570 Rv remove Bam
     primer_bind     8959..8989
                     /label=BP326 Primer YPet into F7 Fw
     primer_bind     8964..9032
                     /label=BP306 mVenus into F7 Fw
     primer_bind     complement(8976..8992)
                     /label=BP094 Fusion PCR PGK-Puro 02 Rev
     primer_bind     8978..9032
                     /label=BP 219 EKAREVnls to pLV Fw
     primer_bind     8978..9017
                     /label=BP 225 EKAREVnls to pLV Fw
     primer_bind     8983..9030
                     /label=BP197 Flag-mNeon into F2 Fw
     primer_bind     8994..9017
                     /label=BP 226 EKAREVnls to E4 (or EF1a) Fw
     primer_bind     complement(9003..9022)
                     /label=BP336 Seq multi-acceptor Rev
     primer_bind     9003..9022
                     /label=BP335 Seq multi-acceptor Fw
     misc_feature    9006..9011
                     /label=EcoRI
     primer_bind     9013..9032
                     /label=BP460 Scarlet-I into K19 Fw
     primer_bind     9013..9032
                     /label=BP543 Fw mTurq2 into F7
     primer_bind     9013..9032
                     /label=BP571 Fw  Prepare TV27
     primer_bind     9014..9035
                     /label=BP 050 Lgr5BAC_HA1_GFP_fw
     primer_bind     9014..9033
                     /label=BP317 Make Q2 PCR2 Fw
     primer_bind     9014..9032
                     /label=BP464 Scarlet-I into K9 PCR2 Fw
     primer_bind     9014..9032
                     /label=BP 221 EKAREVnls to E4 (or EF1a) Fw
     primer_bind     9014..9032
                     /label=BP583 Fw K4-EF1a-mMaroon
     primer_bind     9014..9032
                     /label=BP323 mVenus into F11 Fw
     primer_bind     9014..9032
                     /label=BP081 GFP in pCS2 Fw
     primer_bind     9014..9031
                     /label=BP157 mTurq2-NES into C2 Fw
     primer_bind     9014..9030
                     /label=BP193 mNeon into CFP-CX Fw
     primer_bind     9014..9030
                     /label=BP458 Scarlet-I into K4 Fw
     primer_bind     9014..9030
                     /label=BP185 mNeon into K1 Fw
     primer_bind     9014..9030
                     /label=BP195 mNeon-CAAX into K5 Fw
     primer_bind     9014..9029
                     /label=BP468 Make TV22 PCR1 Fw
                     /note="(67 bp)"
     misc_feature    9015..9725
                     /label=YPet
     primer_bind     9015..9032
                     /label=BP401 Fragm2_Fw
     primer_bind     9015..9032
                     /label=BP587 Fw Fucci4 Gem+cdt1
     primer_bind     9015..9032
                     /label=BP393 mCh-IRES to T24 Fw
     primer_bind     9015..9032
                     /label=BP605 Fw
     primer_bind     9015..9032
                     /label=BP201 GFP-CENP-B into K10 Fw
     primer_bind     9015..9031
                     /label=BP159 mCherry-CAAX to C8-Y+C  Fw
     primer_bind     9018..9035
                     /label=BP508 Fw mCherry into F33
     primer_bind     9018..9035
                     /label=BP665 Fw insert mCherry
     primer_bind     9082..9101
                     /label=BP 010 seq GFP Rev70 (MAAR IS FORWARD!!)
     primer_bind     complement(9089..9107)
                     /label=BP 053 GFP_HA1_control_rev
     primer_bind     complement(9136..9152)
                     /label=BP 009 seq GFP Rev120  (JA, IS WEL ECHT REVERSE)
     primer_bind     complement(9142..9165)
                     /label=BP360 Make Q8c Rev
     primer_bind     9554..9573
                     /label=BP 007 seq GFP Fw540
     primer_bind     complement(9720..9739)
                     /label=BP327 Primer YPet into F7 Rev
     misc_feature    9732..9896
                     /label=WW domain
     primer_bind     complement(9850..9867)
                     /label=BP334 Seq EKAREV from WW
     primer_bind     9883..9901
                     /label=BP537 Fw delete-FQFP
     primer_bind     9889..9905
                     /label=BP573 Fw  extend linker
     primer_bind     9894..9911
                     /label=BP662 Fw Make F30bc
     primer_bind     complement(9907..9926)
                     /label=BP375 Mut F7 S413P+G414S Rev????????
     primer_bind     complement(9907..9926)
                     /label=BP373 Mut F7 G414S Rev????????
     misc_feature    9909..10124
                     /label=EV linker
     misc_feature    9909..9920
                     /label=Feature 23
     primer_bind     complement(9912..9926)
                     /label=BP458 Rv Mut F7 P415G Rev????????
     primer_bind     complement(9919..9944)
                     /label=BP375 Mut F7 S413P+G414S Rev????????
     primer_bind     complement(9919..9944)
                     /label=BP373 Mut F7 G414S Rev????????
     misc_feature    9921..9932
                     /label=Feature 24
     primer_bind     complement(9924..9943)
                     /label=BP458 Rv Mut F7 P415G Rev????????
     primer_bind     complement(9933..9943)
                     /label=BP664 Rv Make F30c
     misc_feature    9933..9938
                     /label=Feature 25
     primer_bind     complement(9937..9956)
                     /label=BP466 Rev linker length 2
     misc_feature    9939..9950
                     /label=Feature 43
     primer_bind     complement(9949..9968)
                     /label=BP375 Mut F7 S413P+G414S Rev????????
     primer_bind     complement(9949..9968)
                     /label=BP373 Mut F7 G414S Rev????????
     misc_feature    9951..9962
                     /label=Feature 32
     primer_bind     complement(9954..9968)
                     /label=BP458 Rv Mut F7 P415G Rev????????
     primer_bind     complement(9961..9986)
                     /label=BP375 Mut F7 S413P+G414S Rev????????
     primer_bind     complement(9961..9986)
                     /label=BP373 Mut F7 G414S Rev????????
     misc_feature    9963..9980
                     /label=Feature 46
     primer_bind     complement(9966..9989)
                     /label=BP663 Rv Make F30b
     primer_bind     complement(9967..9986)
                     /label=BP465 Rev linker length 1
     primer_bind     9970..9983
                     /label=BP662 Fw Make F30bc
     primer_bind     complement(9979..9998)
                     /label=BP375 Mut F7 S413P+G414S Rev????????
     primer_bind     complement(9979..9998)
                     /label=BP373 Mut F7 G414S Rev????????
     misc_feature    9981..9992
                     /label=Feature 33
     primer_bind     complement(9984..9998)
                     /label=BP458 Rv Mut F7 P415G Rev????????
     primer_bind     complement(9991..10010)
                     /label=BP375 Mut F7 S413P+G414S Rev????????
     primer_bind     complement(9991..10010)
                     /label=BP373 Mut F7 G414S Rev????????
     misc_feature    9993..10004
                     /label=Feature 34
     primer_bind     complement(9996..10010)
                     /label=BP458 Rv Mut F7 P415G Rev????????
     primer_bind     complement(10003..10022)
                     /label=BP375 Mut F7 S413P+G414S Rev????????
     primer_bind     complement(10003..10022)
                     /label=BP373 Mut F7 G414S Rev????????
     misc_feature    10005..10016
                     /label=Feature 35
     primer_bind     complement(10008..10022)
                     /label=BP458 Rv Mut F7 P415G Rev????????
     primer_bind     complement(10015..10040)
                     /label=BP375 Mut F7 S413P+G414S Rev????????
     primer_bind     complement(10015..10040)
                     /label=BP373 Mut F7 G414S Rev????????
     misc_feature    10017..10028
                     /label=Feature 36
     primer_bind     complement(10020..10039)
                     /label=BP458 Rv Mut F7 P415G Rev????????
     misc_feature    10029..10046
                     /label=Feature 44
     primer_bind     complement(10029..10039)
                     /label=BP664 Rv Make F30c
     primer_bind     complement(10033..10052)
                     /label=BP466 Rev linker length 2
     primer_bind     complement(10045..10064)
                     /label=BP375 Mut F7 S413P+G414S Rev????????
     primer_bind     complement(10045..10064)
                     /label=BP373 Mut F7 G414S Rev????????
     misc_feature    10047..10058
                     /label=Feature 37
     primer_bind     complement(10050..10064)
                     /label=BP458 Rv Mut F7 P415G Rev????????
     primer_bind     complement(10057..10082)
                     /label=BP375 Mut F7 S413P+G414S Rev????????
     primer_bind     complement(10057..10082)
                     /label=BP373 Mut F7 G414S Rev????????
     misc_feature    10059..10076
                     /label=Feature 47
     primer_bind     complement(10062..10085)
                     /label=BP663 Rv Make F30b
     primer_bind     complement(10063..10082)
                     /label=BP465 Rev linker length 1
     primer_bind     10066..10079
                     /label=BP662 Fw Make F30bc
     primer_bind     complement(10075..10094)
                     /label=BP375 Mut F7 S413P+G414S Rev????????
     primer_bind     complement(10075..10094)
                     /label=BP373 Mut F7 G414S Rev????????
     misc_feature    10077..10088
                     /label=Feature 38
     primer_bind     complement(10080..10094)
                     /label=BP458 Rv Mut F7 P415G Rev????????
     misc_feature    10089..10100
                     /label=Feature 39
     misc_feature    10101..10112
                     /label=Feature 40
     primer_bind     10111..10160
                     /label=BP374 Mut F7 S413P+G414S Fw?????????
     primer_bind     10111..10160
                     /label=BP372 Mut F7 G414S Fw?????????
     primer_bind     complement(10111..10160)
                     /label=BP375 Mut F7 S413P+G414S Rev????????
     primer_bind     complement(10111..10160)
                     /label=BP373 Mut F7 G414S Rev????????
     misc_feature    10113..10124
                     /label=Feature 41
     primer_bind     complement(10116..10163)
                     /label=BP458 Rv Mut F7 P415G Rev????????
     primer_bind     complement(10125..10142)
                     /label=BP664 Rv Make F30c
     primer_bind     complement(10128..10145)
                     /label=BP580 Rv  extend linker
     primer_bind     complement(10129..10190)
                     /label=BP414 Mut F7 D423K, R419S Rv
     primer_bind     10129..10190
                     /label=BP413 Mut F7 D423K,R419S Fw
     primer_bind     complement(10129..10148)
                     /label=BP582 Rv  2xLinker
     primer_bind     complement(10130..10193)
                     /label=BP546 Mut F7 V422T Rv
     primer_bind     10130..10193
                     /label=BP545 Mut F7 V422T Fw
     primer_bind     complement(10132..10175)
                     /label=BP596 Rv Make EKAREN5-TA
     primer_bind     complement(10132..10171)
                     /label=BP392 Mut F7-PW TA Rv
     primer_bind     10132..10171
                     /label=BP391 Mut F7-PW TA Fw
     primer_bind     complement(10132..10163)
                     /label=BP357 F7-P-mimetic E Rev
     primer_bind     complement(10132..10163)
                     /label=BP355 F7-P-mimetic D Rev
     primer_bind     complement(10134..10208)
                     /label=BP461 Rv V422T in PW
     primer_bind     complement(10134..10190)
                     /label=BP377 Mut F7 V422T Rv
     primer_bind     10134..10190
                     /label=BP376 Mut F7 V422T Fw
     misc_feature    10137..10190
                     /label=ERK Substrate
     primer_bind     complement(10137..10190)
                     /label=BP412 Mut F7 D423K Rv
     primer_bind     10137..10190
                     /label=BP411 Mut F7 D423K Fw
     primer_bind     complement(10138..10189)
                     /label=BP408 Mut F7 PW-to-W Rv
     primer_bind     10138..10189
                     /label=BP407 Mut F7 PW-to-W Fw
     primer_bind     complement(10140..10194)
                     /label=BP386 Mut F7 KK424,426PE Rv
     primer_bind     10140..10194
                     /label=BP385 Mut F7 KK424,426PE Fw
     primer_bind     complement(10140..10194)
                     /label=BP384 Mut F7 K426E Rv
     primer_bind     10140..10194
                     /label=BP383 Mut F7 K426E Fw
     primer_bind     complement(10140..10194)
                     /label=BP382 Mut F7 K426W Rv
     primer_bind     10140..10194
                     /label=BP381 Mut F7 K426W Fw
     primer_bind     complement(10140..10194)
                     /label=BP380 Mut F7 KK424,426PW Rv
     primer_bind     10140..10194
                     /label=BP379 Mut F7 KK424,426PW Fw
     primer_bind     complement(10140..10194)
                     /label=BP371 Mut F7 KK424,426PP Rv
     primer_bind     10140..10194
                     /label=BP370 Mut F7 KK424,426PP Fw
     primer_bind     complement(10140..10189)
                     /label=BP367 Mut F7 K424P Rev??????
     primer_bind     10140..10189
                     /label=BP366 Mut F7 K424P Fw?????? ???????????????
     primer_bind     complement(10143..10194)
                     /label=BP369 Mut F7 K426P Rev????????
     primer_bind     10143..10194
                     /label=BP368 Mut F7 K426P Fw?????????
     primer_bind     complement(10145..10178)
                     /label=BP460 Rv Mut F7 L427K Rev????????
     primer_bind     complement(10146..10208)
                     /label=BP464 Rv L427K in F27
     primer_bind     complement(10146..10194)
                     /label=BP410 Mut F7 PW-to-P Rv
     primer_bind     10146..10194
                     /label=BP409 Mut F7 PW-to-P Fw
     misc_feature    10152..10154
                     /label=fosf.site
     primer_bind     complement(10153..10208)
                     /label=BP463 Rv L427E in PW
     primer_bind     complement(10153..10208)
                     /label=BP462 Rv L427W in PW
     primer_bind     10153..10199
                     /label=BP435 Mut F7 Dock F429D Fw
     primer_bind     10155..10205
                     /label=BP415 Mut F7 No Dock Fw
     CDS             10164..10166
                     /label=P
     CDS             10170..10172
                     /label=W
     primer_bind     complement(10194..10213)
                     /label=BP574 Rv  extend linker
     primer_bind     10194..10213
                     /label=BP332 Primer Turq2 into F20 Fw
     primer_bind     10194..10213
                     /label=BP364 Make F22 Fw
     primer_bind     10199..10223
                     /label=BP421 Turq2 to YERC Frgm2 Fw
     CDS             10200..10910
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)Mutation K206A = monomeric to sticky
                     Mutation V224L = enhances dimerization sysynergistically 
                     with S208F of YPet (Vinkenborg. J.L. et al., 2007)
                     "
                     /label=Turquoise2 FRET-optimized
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFLTAAGITLGMDEL"
     primer_bind     10230..10254
                     /label=BP405 Turq2 van F7 into miniTol-Y-ER-C_Fw
     variation       10395..10397
                     /label=T65S , mTurq
     variation       10398..10400
                     /label=Y66W
     variation       10416..10418
                     /label=S72A
     variation       10587..10589
                     /label=G129D
     misc_feature    10638..10640
                     /label=I146F , mTurq2
     variation       10659..10661
                     /label=M153T
     variation       10689..10691
                     /label=V163A
     primer_bind     complement(10716..10733)
                     /label=BP363 Seq Rev vanaf mutated Turq
     primer_bind     10716..10733
                     /label=BP345 Seq Fw vanaf mutated Turq
     variation       10725..10727
                     /label=S175G
     misc_feature    10818..10820
                     /label=K206A monomeric->sticky
     variation       10872..10874
                     /label=V224L enhances dimerization
     primer_bind     complement(10893..10911)
                     /label=BP422 Turq2 to YERC Frgm2 Rev
     misc_feature    10911..10915
                     /label=eCFP
ORIGIN
        1 cctaagaaga agcgtaaggt gtaagtcgac ggtcgagtcg agacgctagc gctagcctag
       61 tgccatttgt tcagtggttc gtagggcttt cccccactgt ttggctttca gttatatgga
      121 tgatgtggta ttgggggcca agtctgtaca gcatcttgag tcccttttta ccgctgttac
      181 caattttctt ttgtctttgg gtaaacccgg gaattccgcc cctctccccc cctaacgtta
      241 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
      301 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
      361 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
      421 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
      481 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
      541 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
      601 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
      661 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
      721 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
      781 gataatatgg gataatatgg ccacaacggt cgccaccatg accgagtaca agcccacggt
      841 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
      901 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
      961 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     1021 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     1081 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     1141 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     1201 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     1261 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     1321 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     1381 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg
     1441 cccgaccgaa aggagcgcac gaccccatgc atgtattcaa tctaagcagg ctttcacttt
     1501 ctcgccaact tacaaggcct ttctgtgtaa acaatacctg aacctttacc ccgttgcccg
     1561 gcaacggcca ggtctgtgcc aagtgtttgc tgacgcaacc cccactggct ggggcttggt
     1621 catgggccat cagcgcatgc gtggaacctt ttcggctcct ctgccgatcc atactgcgga
     1681 actcctagcc gcttgttttg ctcgcagcag gtctggagca aacattatcg ggactgataa
     1741 ctctgttgtc ctatcccgca aatatacatc gtttccatgg ctgctaggct gtgctgccaa
     1801 ctggatcctg cgcgggacgt cctttgttta cgtcccgtcg gcgctgaatc ctgcggacga
     1861 cccttctcgg ggtcgcttgg gactctctcg tccccttctc cgtctgccgt tccgaccgac
     1921 cacggggcgc acctctcttt acgcggactc cccgtctgtg ccttctcatc tgccggaccg
     1981 tgtgcacttc gcttcacctc tgcacgtcgc atggagacca ccgtgaacgc ccaccaaata
     2041 ttgcccaagg tcttacataa gaggactctt ggactctcag caatgtcaac gaccgacctt
     2101 gaggcatact tcaaagactg tttgtttaaa gactgggagg agttggggga ggagattagg
     2161 ttaaaggtct ttgtactagg aggctgtagg cataaattgg tctgcgcacc agcaccatgt
     2221 atcactagag cggggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     2281 actttttaaa agaaaagggg ggacttggaa gggctaattc actcccaacg aagacaagat
     2341 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     2401 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     2461 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     2521 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     2581 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     2641 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     2701 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     2761 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     2821 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     2881 gaggcctagg cttttgcgtc gagacgtacc caattcgccc tatagtgagt cgtattacgc
     2941 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     3001 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     3061 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag
     3121 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3181 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3241 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3301 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     3361 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     3421 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     3481 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     3541 caaaatatta acgtttacaa tttcccaggt ggcacttttc ggggaaatgt gcgcggaacc
     3601 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3661 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3721 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3781 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3841 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3901 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3961 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     4021 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     4081 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     4141 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     4201 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4261 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4321 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4381 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4441 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4501 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     4561 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4621 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4681 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4741 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4801 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4861 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4921 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4981 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5041 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5101 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5161 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5221 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5281 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5341 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     5401 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     5461 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     5521 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     5581 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     5641 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     5701 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     5761 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     5821 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     5881 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     5941 ggattggacg aaccactgaa ttggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     6001 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     6061 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6121 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6181 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     6241 ctgaaagcga aagggaaacc agagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     6301 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     6361 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     6421 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     6481 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     6541 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     6601 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     6661 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     6721 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     6781 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     6841 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     6901 gttcttggga gcagcaggaa gcactatggg cgcagcctca atgacgctga cggtacaggc
     6961 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     7021 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     7081 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     7141 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     7201 gattggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     7261 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     7321 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     7381 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     7441 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     7501 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     7561 agagacagat ccattcgatt agtgaacgga tctcgacggt atcggtttta aaagaaaagg
     7621 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     7681 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatcgatc atatgactag
     7741 agcggcctta cgacattgat tattgactag ggctccggtg cccgtcagtg ggcagagcgc
     7801 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
     7861 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     7921 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     7981 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
     8041 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctccagta cgtgattctt
     8101 gatcccgagc tggagccagg ggcgggcctt gcgctttagg agccccttcg cctcgtgctt
     8161 gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
     8221 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
     8281 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca ggatctgcac actggtattt
     8341 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
     8401 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
     8461 ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
     8521 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctccagggg
     8581 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
     8641 aaggggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt
     8701 ccaggcacct cgattagttc tggagctttt ggagtacgtc gtctttaggt tggggggagg
     8761 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt
     8821 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc
     8881 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgattaatta
     8941 aacgcgtacc gatagcttcg aattctgcag tcgacgatat cactagtctc gagcattttg
     9001 gcaaagaatt cggcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     9061 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     9121 gcgatgccac ctacggcaag ctgaccctga agcttctatg caccaccggc aagctgcccg
     9181 tgccctggcc caccctcgtg accaccctgg gctacggcct gcagtgcttc gcccgctacc
     9241 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     9301 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     9361 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     9421 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcaccgccg
     9481 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg
     9541 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     9601 tgcccgacaa ccactacctg agctaccagt ccgccctgtt caaagacccc aacgagaagc
     9661 gcgatcacat ggtcctgctg gagttcctga ccgccgccgg gatcactgag ggcatgaacg
     9721 agctgtacct cgagatggcg gacgaggaga agctgccgcc cggctgggag aagcgcatga
     9781 gccgcagctc aggccgagtg tactacttca accacatcac taacgccagc cagtgggagc
     9841 ggcccagcgg caacagcagc agtggtggca aaaacgggca gggggagcct gccaggggta
     9901 ccagtgctgg tggtagtgct ggtggtagtg ctggtggttc cggcagtgct ggtggtagtg
     9961 ctggtggtag taccagtgct ggtggtagtg ctggtggtag tgctggtggt agtgctggtg
    10021 gtagtgctgg tggttccggc agtgctggtg gtagtgctgg tggtagtacc agtgctggtg
    10081 gtagtgctgg tggtagtgct ggtggtagtg ctggtggtag tgctggtggt tccggaccag
    10141 atgtccctag agctccagtg gatccagcat ggctgtcatt ccaatttccg ggcggccgca
    10201 tggtgagtaa aggggaagaa ctgtttactg gcgtggtgcc aattctggtc gaactggatg
    10261 gagatgtaaa tgggcataaa tttagtgtgt ctggagaagg ggaaggagac gctacatatg
    10321 ggaaactgac actgaaattt atttgtacta ctggaaaact gcctgtgcct tggcctacac
    10381 tcgtgacaac cctgtcttgg ggagtgcagt gttttgcccg gtatcctgat catatgaaac
    10441 agcatgattt tttcaaatct gccatgcctg agggatatgt ccaggaacgg acaattttct
    10501 ttaaagatga tgggaattat aaaacacggg ccgaagtgaa atttgaaggg gatacactgg
    10561 tgaatcggat tgaactgaaa ggaattgatt ttaaagaaga tgggaatatt ctgggccata
    10621 aactggaata taattatttc agcgataatg tctacattac agccgataaa cagaagaatg
    10681 ggattaaagc caattttaaa attcggcata atattgaaga tggaggggtg cagctcgccg
    10741 atcattatca gcagaataca cctattgggg atgggcctgt gctgctgcct gataatcatt
    10801 atctgagcac acagtctgcc ctgagcaagg accctaatga aaagcgggac catatggtcc
    10861 tgctggaatt tctgactgcc gccggcatta cactcgggat ggatgaactg tctaga
//
