LOCUS       Exported               11032 bp ds-DNA     circular SYN 26-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11032)
  AUTHORS   UMC-Utrecht
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 26, 2021 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11032
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..21
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     206..229
                     /label=GK228 pLV-IRES-Gib-F
     misc_feature    220..790
                     /label=IRES
     primer_bind     complement(358..377)
                     /label=BP128 IRES Seq 160 Rev
     primer_bind     358..377
                     /label=BP127 IRES Seq 160 Fw
     primer_bind     366..381
                     /label=Primer 15
     primer_bind     666..684
                     /label=Primer 16
     primer_bind     686..705
                     /label=BP129 IRES Seq 490 Fw
     primer_bind     complement(774..817)
                     /label=GK230 IRES-Neo-Gib-R
     primer_bind     complement(774..816)
                     /label=GK229 IRES-Blas-Gib-R
     misc_feature    791..796
                     /label=IRES
     primer_bind     808..833
                     /label=BP095 Fusion PCR PGK-Puro 03 Fw
     CDS             814..1413
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(943..961)
                     /label=JP030
     primer_bind     complement(949..968)
                     /label=BP123 Puro Seq Rev140
     primer_bind     951..969
                     /label=JP006
     primer_bind     951..969
                     /label=KO fw-Puro-seq.primer #37
     primer_bind     1213..1230
                     /label=BP121 Puro Seq Fw400
     primer_bind     complement(1213..1230)
                     /label=BP124 Puro Seq Rev410
     primer_bind     1310..1329
                     /label=BP122 Puro Seq Fw510
     primer_bind     complement(1574..1592)
                     /label=KO rv-pLV_Puro/end-seq.primer #19
     LTR             2302..2535
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2607..2728
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2641..2664)
                     /label=BP190 Seq Rev into TagGFP of Casper3
     rep_origin      2747..2882
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(2915..2933)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2943..2959)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3104..3559
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3588..3692
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3693..4553
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4519..4538
                     /label=BP 048 Amp_HA2_control_fw
     rep_origin      4724..5312
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5600..5621
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     5620..5639
                     /label=BP258 Seq pr in miniTol vector (T2) Rev (2)
     primer_bind     5620..5639
                     /label=BP258 Seq pr in miniTol vector (T2) Rev
     promoter        5636..5666
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5674..5690
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5698..5714
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5735..5753
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             6037..6217
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6261..6386
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     primer_bind     6405..6424
                     /label=BP 001 seq TGF-RE Fw1
     primer_bind     6618..6638
                     /label=BP 002 seq TGF-RE Fw2
     primer_bind     6659..6681
                     /label=BP 003 seq TGF-RE Fw3
     misc_feature    6879..7112
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     primer_bind     7331..7350
                     /label=BP 022 seq TGF-RE Fw4
     primer_bind     7458..7477
                     /label=BP 023 seq TGF-RE Fw5
     primer_bind     complement(7474..7493)
                     /label=KO rv-pLV-seq.primer #38
     primer_bind     7518..7537
                     /label=BP 024 seq TGF-RE Fw6
     primer_bind     7560..7578
                     /label=KO fw-pLV_TOP5-seq.primer #16
     primer_bind     7579..7598
                     /label=BP 025 seq TGF-RE Fw7
     misc_feature    7602..7719
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7767..8929
                     /label=hEF1 promoter
     primer_bind     complement(7823..7853)
                     /label=GK228 pLV-IRES-Gib-F
     primer_bind     8857..8876
                     /label=KO fw-hEf1a(end) #88
     misc_feature    9002..9007
                     /label=EcoRI
     misc_feature    9011..9721
                     /label=YPet
     misc_feature    9728..9892
                     /label=WW domain
     misc_feature    9905..10240
                     /label=EV linker
     misc_feature    9905..9916
                     /label=Feature 23
     misc_feature    9917..9928
                     /label=Feature 24
     misc_feature    9929..9940
                     /label=Feature 25
     misc_feature    9941..9952
                     /label=Feature 26
     misc_feature    9953..9970
                     /label=Feature 42
     misc_feature    9971..9982
                     /label=Feature 27
     misc_feature    9983..10000
                     /label=Feature 45
     misc_feature    10001..10012
                     /label=Feature 28
     misc_feature    10013..10024
                     /label=Feature 29
     misc_feature    10025..10036
                     /label=Feature 30
     misc_feature    10037..10048
                     /label=Feature 31
     misc_feature    10049..10066
                     /label=Feature 43
     misc_feature    10067..10078
                     /label=Feature 32
     misc_feature    10079..10096
                     /label=Feature 46
     misc_feature    10097..10108
                     /label=Feature 33
     misc_feature    10109..10120
                     /label=Feature 34
     misc_feature    10121..10132
                     /label=Feature 35
     misc_feature    10133..10144
                     /label=Feature 36
     misc_feature    10145..10162
                     /label=Feature 44
     misc_feature    10163..10174
                     /label=Feature 37
     misc_feature    10175..10192
                     /label=Feature 47
     misc_feature    10193..10204
                     /label=Feature 38
     misc_feature    10205..10216
                     /label=Feature 39
     misc_feature    10217..10228
                     /label=Feature 40
     misc_feature    10229..10240
                     /label=Feature 41
     misc_feature    10253..10306
                     /label=ERK Substrate
     misc_feature    10268..10270
                     /label=fosf.site
     CDS             10280..10282
                     /label=P
     CDS             10286..10288
                     /label=W
     misc_feature    10299..10313
                     /label=Infusion homology arm
     CDS             10316..11026
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)Mutation K206A = monomeric to sticky
                     Mutation V224L = enhances dimerization sysynergistically 
                     with S208F of YPet (Vinkenborg. J.L. et al., 2007)
                     "
                     /label=Turquoise2 FRET-optimized
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFLTAAGITLGMDEL"
     variation       10511..10513
                     /label=T65S , mTurq
     variation       10514..10516
                     /label=Y66W
     variation       10532..10534
                     /label=S72A
     variation       10703..10705
                     /label=G129D
     misc_feature    10754..10756
                     /label=I146F , mTurq2
     variation       10775..10777
                     /label=M153T
     variation       10805..10807
                     /label=V163A
     variation       10841..10843
                     /label=S175G
     misc_feature    10934..10936
                     /label=K206A monomeric->sticky
     variation       10988..10990
                     /label=V224L enhances dimerization
     misc_feature    11027..11031
                     /label=eCFP
ORIGIN
        1 cctaagaaga agcgtaaggt gtaagtcgac ggtcgagtcg agacgctagc gctagcctag
       61 tgccatttgt tcagtggttc gtagggcttt cccccactgt ttggctttca gttatatgga
      121 tgatgtggta ttgggggcca agtctgtaca gcatcttgag tcccttttta ccgctgttac
      181 caattttctt ttgtctttgg gtaaacccgg gaattccgcc cctctccccc cctaacgtta
      241 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
      301 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
      361 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
      421 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
      481 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
      541 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
      601 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
      661 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
      721 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
      781 gataatatgg atatggccac aacggtcgcc accatgaccg agtacaagcc cacggtgcgc
      841 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
      901 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
      961 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     1021 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     1081 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     1141 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     1201 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     1261 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     1321 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     1381 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
     1441 accgaaagga gcgcacgacc ccatgcatgt attcaatcta agcaggcttt cactttctcg
     1501 ccaacttaca aggcctttct gtgtaaacaa tacctgaacc tttaccccgt tgcccggcaa
     1561 cggccaggtc tgtgccaagt gtttgctgac gcaaccccca ctggctgggg cttggtcatg
     1621 ggccatcagc gcatgcgtgg aaccttttcg gctcctctgc cgatccatac tgcggaactc
     1681 ctagccgctt gttttgctcg cagcaggtct ggagcaaaca ttatcgggac tgataactct
     1741 gttgtcctat cccgcaaata tacatcgttt ccatggctgc taggctgtgc tgccaactgg
     1801 atcctgcgcg ggacgtcctt tgtttacgtc ccgtcggcgc tgaatcctgc ggacgaccct
     1861 tctcggggtc gcttgggact ctctcgtccc cttctccgtc tgccgttccg accgaccacg
     1921 gggcgcacct ctctttacgc ggactccccg tctgtgcctt ctcatctgcc ggaccgtgtg
     1981 cacttcgctt cacctctgca cgtcgcatgg agaccaccgt gaacgcccac caaatattgc
     2041 ccaaggtctt acataagagg actcttggac tctcagcaat gtcaacgacc gaccttgagg
     2101 catacttcaa agactgtttg tttaaagact gggaggagtt gggggaggag attaggttaa
     2161 aggtctttgt actaggaggc tgtaggcata aattggtctg cgcaccagca ccatgtatca
     2221 ctagagcggg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     2281 tttaaaagaa aaggggggac ttggaagggc taattcactc ccaacgaaga caagatctgc
     2341 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     2401 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     2461 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     2521 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     2581 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     2641 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     2701 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     2761 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     2821 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     2881 cctaggcttt tgcgtcgaga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     2941 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     3001 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     3061 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc
     3121 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3181 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3241 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3301 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     3361 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     3421 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     3481 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     3541 atattaacgt ttacaatttc ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3601 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3661 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3721 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     3781 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3841 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     3901 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     3961 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4021 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4081 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4141 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4201 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     4261 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4321 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     4381 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     4441 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     4501 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4561 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4621 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4681 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4741 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4801 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4861 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4921 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4981 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5041 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5101 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5161 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5221 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5281 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5341 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5401 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     5461 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     5521 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     5581 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     5641 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     5701 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     5761 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     5821 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     5881 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     5941 tggacgaacc actgaattgg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     6001 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     6061 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6121 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6181 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacctga
     6241 aagcgaaagg gaaaccagag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     6301 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     6361 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     6421 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     6481 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     6541 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     6601 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     6661 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     6721 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     6781 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     6841 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     6901 ttgggagcag caggaagcac tatgggcgca gcctcaatga cgctgacggt acaggccaga
     6961 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     7021 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     7081 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     7141 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     7201 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     7261 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     7321 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     7381 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     7441 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     7501 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     7561 acagatccat tcgattagtg aacggatctc gacggtatcg gttttaaaag aaaagggggg
     7621 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     7681 aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgatcatat gactagagcg
     7741 gccttacgac attgattatt gactagggct ccggtgcccg tcagtgggca gagcgcacat
     7801 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     7861 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     7921 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     7981 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     8041 gttatggccc ttgcgtgcct tgaattactt ccacctggct ccagtacgtg attcttgatc
     8101 ccgagctgga gccaggggcg ggccttgcgc tttaggagcc ccttcgcctc gtgcttgagt
     8161 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
     8221 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
     8281 ttttttctgg caagatagtc ttgtaaatgc gggccaggat ctgcacactg gtatttcggt
     8341 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
     8401 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     8461 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     8521 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctc cagggggctc
     8581 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaagg
     8641 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     8701 gcacctcgat tagttctgga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     8761 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     8821 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     8881 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgat taattaaacg
     8941 cgtaccgata gcttcgaatt ctgcagtcga cgatatcact agtctcgagc attttggcaa
     9001 agaattcggc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     9061 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     9121 tgccacctac ggcaagctga ccctgaagct tctatgcacc accggcaagc tgcccgtgcc
     9181 ctggcccacc ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga
     9241 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     9301 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     9361 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     9421 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa
     9481 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt
     9541 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     9601 cgacaaccac tacctgagct accagtccgc cctgttcaaa gaccccaacg agaagcgcga
     9661 tcacatggtc ctgctggagt tcctgaccgc cgccgggatc actgagggca tgaacgagct
     9721 gtacctcgag atggcggacg aggagaagct gccgcccggc tgggagaagc gcatgagccg
     9781 cagctcaggc cgagtgtact acttcaacca catcactaac gccagccagt gggagcggcc
     9841 cagcggcaac agcagcagtg gtggcaaaaa cgggcagggg gagcctgcca ggggtaccag
     9901 tgctggtggt agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg ctggtggttc
     9961 cggcagtgct ggtggtagtg ctggtggtag taccagtgct ggtggtagtg ctggtggtag
    10021 tgctggtggt agtgctggtg gtagtgctgg tggttccggc agtgctggtg gtagtgctgg
    10081 tggtagtacc agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg ctggtggtag
    10141 tgctggtggt tccggcagtg ctggtggtag tgctggtggt agtaccagtg ctggtggtag
    10201 tgctggtggt agtgctggtg gtagtgctgg tggtagtgct ggtggttccg gaccagatgt
    10261 ccctagaact ccagtggatc cagcatggct gtcattccaa tttccgggcg gccgcatggt
    10321 gagtaaaggg gaagaactgt ttactggcgt ggtgccaatt ctggtcgaac tggatggaga
    10381 tgtaaatggg cataaattta gtgtgtctgg agaaggggaa ggagacgcta catatgggaa
    10441 actgacactg aaatttattt gtactactgg aaaactgcct gtgccttggc ctacactcgt
    10501 gacaaccctg tcttggggag tgcagtgttt tgcccggtat cctgatcata tgaaacagca
    10561 tgattttttc aaatctgcca tgcctgaggg atatgtccag gaacggacaa ttttctttaa
    10621 agatgatggg aattataaaa cacgggccga agtgaaattt gaaggggata cactggtgaa
    10681 tcggattgaa ctgaaaggaa ttgattttaa agaagatggg aatattctgg gccataaact
    10741 ggaatataat tatttcagcg ataatgtcta cattacagcc gataaacaga agaatgggat
    10801 taaagccaat tttaaaattc ggcataatat tgaagatgga ggggtgcagc tcgccgatca
    10861 ttatcagcag aatacaccta ttggggatgg gcctgtgctg ctgcctgata atcattatct
    10921 gagcacacag tctgccctga gcaaggaccc taatgaaaag cgggaccata tggtcctgct
    10981 ggaatttctg actgccgccg gcattacact cgggatggat gaactgtcta ga
//
