LOCUS       Exported               11293 bp ds-DNA     circular SYN 26-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11293)
  AUTHORS   UMC-Utrecht
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 26, 2021 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11293
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(94..107)
                     /label=BP124 Puro Seq Rev410
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     primer_bind     825..844
                     /label=BP 001 seq TGF-RE Fw1
                     /note=";sequence TGF-betaRE-NLS-RFP / PGK:H2B-CFP;;;;;;;"
     primer_bind     1038..1058
                     /label=BP 002 seq TGF-RE Fw2
                     /note=";sequence TGF-betaRE-NLS-RFP / PGK:H2B-CFP;;;;;;;"
     primer_bind     1079..1101
                     /label=BP 003 seq TGF-RE Fw3
                     /note=";sequence TGF-betaRE-NLS-RFP / PGK:H2B-CFP;;;;;;;"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     primer_bind     1756..1775
                     /label=BP 022 seq TGF-RE Fw4
                     /note=";sequence TGF-betaRE-NLS-RFP   and also the 
                     BMP-RE:ZsGreen etc;bindt in seq-resultaat van BP001-BP003 .
                     Daar was nog geen TGF-RE in te vinden;;;;;;"
     primer_bind     1883..1902
                     /label=BP 023 seq TGF-RE Fw5
                     /note=";sequence TGF-betaRE-NLS-RFP   and also the 
                     BMP-RE:ZsGreen etc;bindt in seq-resultaat van BP001-BP003 .
                     Daar was nog geen TGF-RE in te vinden;;;;;;"
     primer_bind     1943..1962
                     /label=BP 024 seq TGF-RE Fw6
                     /note=";sequence TGF-betaRE-NLS-RFP   and also the 
                     BMP-RE:ZsGreen etc;bindt in seq-resultaat van BP001-BP003 .
                     Daar was nog geen TGF-RE in te vinden;;;;;;"
     primer_bind     2004..2023
                     /label=BP 025 seq TGF-RE Fw7
                     /note=";sequence TGF-betaRE-NLS-RFP   and also the 
                     BMP-RE:ZsGreen etc;bindt in seq-resultaat van BP001-BP003 .
                     Daar was nog geen TGF-RE in te vinden;;;;;;"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2189..2208
                     /label=BP338 2x-Lock into Q5 Fw
     protein_bind    2230..2500
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2576..2592)
                     /label=BP 026 seq TGF-RE Rv4
                     /note=";sequence TGF-betaRE-NLS-RFP   and also the 
                     BMP-RE:ZsGreen etc;bindt reverse in min CMV promoter;;;;;;"
     misc_feature    2654..2666
                     /label=niet in oorspr seq
     primer_bind     2667..2699
                     /label=BP301 P3 Fragment1 Fw
     misc_feature    2673..2676
                     /label=BstXI remnant
     primer_bind     2685..2702
                     /label=BP417 mKate2 into T27 Fw
     misc_feature    2685..2689
                     /label=Kozak
     primer_bind     2689..2707
                     /label=BP182 Casp-Sensor into K1 Fw
     misc_feature    2690..3442
                     /label=mKate2
     primer_bind     2690..2707
                     /label=BP191 Casp-Sensor into K1 Fw
     primer_bind     complement(2932..2951)
                     /label=BP063 Seq TagRFP 260 Rev
     primer_bind     3027..3046
                     /label=BP116 TagRFP Seq Fw 450
     primer_bind     complement(3149..3181)
                     /label=BP218 S158T mutagenesis in K6 Fw
     primer_bind     3149..3181
                     /label=BP217 S158T mutagenesis in K6 Fw
     primer_bind     3302..3321
                     /label=BP117 TagRFP Seq Fw 640
     primer_bind     3352..3371
                     /label=BP118 TagRFP Seq Fw 690
     primer_bind     complement(3362..3376)
                     /label=BP073 TagRFP(no stop) before P2A Rev
     primer_bind     complement(3362..3376)
                     /label=BP282 TagRFP into K15 Rev
     primer_bind     complement(3362..3376)
                     /label=BP293 K19b into K19 Rev
     primer_bind     complement(3362..3376)
                     /label=BP228 TagRFP-T into K15 Rev
     misc_feature    3386..3406
                     /label=NLS
     misc_feature    3416..3436
                     /label=NLS
     primer_bind     complement(3424..3442)
                     /label=BP179 mTFP-2xNLS (no stop) in pCS2 Rev
     primer_bind     complement(3426..3484)
                     /label=BP305 P3 Fragment1Rev
     primer_bind     complement(3426..3442)
                     /label=BP302 P3 Fragment1Rev
     primer_bind     3434..3498
                     /label=BP303 P3 Fragment2 Fw
     misc_feature    3443..3475
                     /label=3rd NLS added by BP
     primer_bind     3476..3496
                     /label=BP348 Make Q10 PCR2 Fw
     CDS             3476..3487
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="ELYK"
     primer_bind     3478..3517
                     /label=BP237 make K8 Fw
     primer_bind     3489..3511
                     /label=BP264 PCR from pJet-Casp-gBlock Fw
     primer_bind     3499..3517
                     /label=BP070 P2A into e.v. Fw
     CDS             3509..3565
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(3542..3570)
                     /label=BP418 mKate2 into T27 Rev
     primer_bind     complement(3542..3564)
                     /label=BP279 half-mTFP+P2A into Y12 Rev
     primer_bind     complement(3544..3569)
                     /label=BP400 Fragm1_Rev
     primer_bind     complement(3544..3565)
                     /label=BP162 H2B-mTurq2 into E2 Rev
                     /note=";Ja, al besteld;;;;;;;"
     primer_bind     complement(3544..3565)
                     /label=BP187 H2B-mTurq2-2A into E2 Rev
     primer_bind     complement(3545..3574)
                     /label=BP316 Make Q2 PCR1 Rev
     primer_bind     complement(3545..3574)
                     /label=Fragment 2.REV
     primer_bind     complement(3545..3566)
                     /label=BP181 H2B-GFP-2A into pLV Rev
     primer_bind     complement(3545..3566)
                     /label=BP236 H2B-G-or-R-P2A into T5 Rev
     primer_bind     complement(3545..3566)
                     /label=BP 112  PCR G2 into NsiI Rev
     primer_bind     complement(3545..3565)
                     /label=BP212 CyclinB-TagRFP into K4 Rev
     primer_bind     complement(3545..3565)
                     /label=BP147 H2B-TagRFP-2A into pLV-ERKsensor Rev
     primer_bind     complement(3545..3565)
                     /label=BP248 H2B-Neon-P2A into T5 Rev
     primer_bind     complement(3548..3565)
                     /label=BP350 Make Q10 PCR2 Rev _02
     misc_feature    3572..4141
                     /label=HRas(N17)
     variation       3620..3622
                     /label=R-N
     primer_bind     complement(4120..4141)
                     /label=BP160 mCherry-CAAX to C8-Y+C  Rev
     CDS             4170..4196
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     4221..4238
                     /label=BP097 Fusion PCR PGK-Puro 05 Fw
     primer_bind     4221..4237
                     /label=BP091 PGK Infusion Fw
     primer_bind     4226..4241
                     /label=BP 042 PGK-Puro-pA Fw
     primer_bind     4226..4241
                     /label=BP169 PGK:Puro w/o polyA s Fw
     primer_bind     4226..4241
                     /label=BP167 PGK:Puro w/o polyA s Fw
     primer_bind     4228..4243
                     /label=BP229 PGK-PuroDTK for T2 Fw
                     /note=";""    PROJECT """"T"""""";;;;;;;"
     primer_bind     4228..4243
                     /label=BP249 PGK-PruDTK for T2 Fw
                     /note=";weeeeeer anders!!!!  NU DEFINITIEF GOED;;;;;;;"
     primer_bind     4229..4243
                     /label=BP239 prepare pLV for project T Fw
                     /note=";DEZE 239 IS BESTELD MAAR FOUT !!!;;;;;;;"
     primer_bind     4229..4243
                     /label=BP233 PGK-Puro into T4 Fw
     primer_bind     4229..4243
                     /label=BP241 prepare pLV for project T Fw
     promoter        4282..5493
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(5416..5451)
                     /label=BP210 UbC into F2 Rev
     primer_bind     5548..5566
                     /label=BP283 TetOn3G into pLV-Neo Fw
     CDS             5552..6298
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5651..5670)
                     /label=BP252 Seq TetOn (T2) 100 Rev
     primer_bind     5651..5670
                     /label=BP251 Seq TetOn (T2) 100 Fw
     primer_bind     complement(5894..5904)
                     /label=BP191 Casp-Sensor into K1 Fw
     primer_bind     complement(5894..5904)
                     /label=BP182 Casp-Sensor into K1 Fw
     primer_bind     complement(6045..6062)
                     /label=BP254 Seq TetOn (T2) 500 Rev
     primer_bind     complement(6240..6259)
                     /label=BP256 Seq TetOn (T2) 700 Rev
     primer_bind     6240..6259
                     /label=BP255 Seq TetOn (T2) 700 Fw
     primer_bind     complement(6278..6298)
                     /label=BP284 TetOn3G into pLV-Neo Rv
     misc_feature    6331..6883
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (ECMV)"
     primer_bind     complement(6454..6473)
                     /label=BP128 IRES Seq 160 Rev
     primer_bind     6454..6473
                     /label=BP127 IRES Seq 160 Fw
     primer_bind     6782..6801
                     /label=BP129 IRES Seq 490 Fw
     primer_bind     complement(6852..6881)
                     /label=BP398 Fragm2_Rev
     primer_bind     complement(6872..6904)
                     /label=BP402 Fragm2_Rev
     primer_bind     complement(6886..6906)
                     /label=BP394 mCh-IRES to T24 Rv
     primer_bind     6904..6929
                     /label=BP095 Fusion PCR PGK-Puro 03 Fw
     primer_bind     6904..6921
                     /label=BP040 Puro Fw
     CDS             6910..7509
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7045..7064)
                     /label=BP123 Puro Seq Rev140
     primer_bind     7309..7326
                     /label=BP121 Puro Seq Fw400
     primer_bind     complement(7309..7326)
                     /label=BP124 Puro Seq Rev410
     primer_bind     7406..7425
                     /label=BP122 Puro Seq Fw510
     primer_bind     complement(7493..7520)
                     /label=BP096 Fusion PCR PGK-Puro 04 Rev
     primer_bind     complement(7493..7510)
                     /label=BP098 Fusion PCR PGK-Puro 06 Rev
     primer_bind     complement(7493..7510)
                     /label=BP041 Puro Rv
     primer_bind     complement(7493..7510)
                     /label=BP234 PGK-Puro into T4 Rev
     primer_bind     complement(7493..7509)
                     /label=BP170 PGK:Puro w/o polyA s Rev
     primer_bind     complement(7493..7509)
                     /label=BP168 PGK:Puro w/o polyA s Rev
     primer_bind     7577..7600
                     /label=BP 043 PGK-Puro-pA Rv
     primer_bind     7577..7600
                     /label=BP077 PGK-Puro-pA Rv
     primer_bind     7577..7598
                     /label=BP296 make S1, polyA into E4, Rev
     primer_bind     7577..7595
                     /label=bp341 TagRFP into Q3 Rev
     primer_bind     complement(7579..7598)
                     /label=BP067 Seq primer polyA Fw
     primer_bind     7579..7598
                     /label=BP066 Seq primer polyA Rev
     misc_feature    7683..8271
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8154..8165)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8346..8579
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        8784..8888
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8889..9749
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9715..9734
                     /label=BP 048 Amp_HA2_control_fw
                     /note=";copied from Bussmann et al, 2010;;;;;;;"
     rep_origin      9920..10508
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    10919..10940
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     10939..10958
                     /label=BP258 Seq pr in miniTol vector (T2) Rev
     promoter        10955..10985
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10993..11009
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagacgc gtttccacca tggtgagcga
     2701 gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtga acaaccacca
     2761 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat
     2821 caaggcggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ccagcttcat
     2881 gtacggcagc aaaaccttca tcaaccacac ccagggcatc cccgacttct ttaagcagtc
     2941 cttccccgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac
     3001 cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg
     3061 ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctc
     3121 caccgagacc ctgtaccccg ctgacggcgg cctggaaggc agagccgaca tggccctgaa
     3181 gctcgtgggc gggggccacc tgatctgcaa cttgaagacc acatacagat ccaagaaacc
     3241 cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac agaagactgg aaagaatcaa
     3301 ggaggccgac aaagagacct acgtcgagca gcacgaggtg gctgtggcca gatactgcga
     3361 cctccctagc aaactggggc acagaccaaa gaaaaagaga aaagtcggag gtggaccaaa
     3421 gaagaagcga aaagttagcg ctccaaaaaa gaaaaggaaa gtcagcatgt taacggagct
     3481 gtacaaggct agagatatcg gatctggcgc cacaaacttc tccctgttga agcaggcggg
     3541 agatgttgaa gagaaccctg gacctgctag catgacggaa tataagcttg tggtggtggg
     3601 cgccggcggt gtgggcaaga atgcgctgac catccagctg atccagaacc attttgtgga
     3661 cgaatacgac cccactatag aggattccta ccggaagcag gtggtcattg atggggagac
     3721 gtgcctgttg gacatcctgg ataccgccgg ccaggaggag tacagcgcca tgcgggacca
     3781 gtacatgcgc accggggagg gcttcctgtg tgtgtttgcc atcaacaaca ccaagtcttt
     3841 tgaggacatc caccagtaca gggagcagat caaacgggtg aaggactcgg atgacgtgcc
     3901 catggtgctg gtggggaaca agtgtgacct ggctgcacgc actgtggaat ctcggcaggc
     3961 tcaggacctc gcccgaagct acggcatccc ctacatcgag acctcggcca agacccggca
     4021 gggagtggag gatgccttct acacgttggt gcgtgagatc cggcagcaca agctgcggaa
     4081 gctgaaccct cctgatgaga gtggccccgg ctgcatgagc tgcaagtgtg tgctctcctg
     4141 actcgagtct agagggcccg cggttcgaat acccatacga cgtcccagac tacgcttagt
     4201 aatgattaat taaactagaa attctaccgg gtaggggagg cgcttttccc aaggcagtct
     4261 ggagtaaccc acccaagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc
     4321 ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt
     4381 ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt
     4441 atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc
     4501 cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc
     4561 cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc
     4621 cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg
     4681 tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac
     4741 ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg
     4801 aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga
     4861 cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag
     4921 aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct
     4981 ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc
     5041 gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg
     5101 agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg
     5161 ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc
     5221 ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg
     5281 aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg
     5341 ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt
     5401 gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct
     5461 aaattctggc cgtttttggc ttttttgtta gacgaagctt ggtaccgagc tcggatctcc
     5521 accccgtacc ggtcctgcag tcgaattcac catgtctaga ctggacaaga gcaaagtcat
     5581 aaacggagct ctggaattac tcaatggtgt cggtatcgaa ggcctgacga caaggaaact
     5641 cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc
     5701 cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct
     5761 ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct
     5821 cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta
     5881 cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc
     5941 actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga
     6001 gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct
     6061 gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct
     6121 ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga
     6181 cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg actttgacct
     6241 tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaact
     6301 aagtaaggat ccgcggccgc atcgatacta acgttactgg ccgaagccgc ttggaataag
     6361 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     6421 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     6481 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     6541 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     6601 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     6661 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     6721 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     6781 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     6841 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggatat ggccacaacg
     6901 gtcgccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     6961 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     7021 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     7081 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     7141 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     7201 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     7261 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     7321 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     7381 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     7441 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     7501 ggtgcctgac gcatgcatgc ggccgcgact ctagatcata atcagccata ccacatttgt
     7561 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     7621 gaatgcaatt gttgttgttt agtccctccc aattcgatat caagcttatc gcgatagatc
     7681 ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     7741 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     7801 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     7861 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     7921 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     7981 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     8041 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     8101 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     8161 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     8221 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc
     8281 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8341 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     8401 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8461 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8521 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8581 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     8641 gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc
     8701 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     8761 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8821 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8881 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     8941 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9001 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9061 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9121 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9181 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9241 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9301 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9361 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9421 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9481 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9541 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9601 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9661 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9721 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9781 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     9841 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     9901 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     9961 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10021 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    10081 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10141 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10201 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10261 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10321 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10381 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10441 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10501 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10561 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    10621 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    10681 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    10741 gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    10801 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    10861 gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    10921 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    10981 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
    11041 cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    11101 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    11161 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
    11221 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    11281 caaacttagt agt
//
