LOCUS       pSEVA221_Ptet_pm        7855 bp    DNA     circular     09-SEP-2019
FEATURES             Location/Qualifiers
     terminator      1..103
                     /note="T0"
     gene            231..1046
                     /gene="neo"
     misc_feature    1078..1323
                     /note="oriT"
     gene            complement(1382..2530)
                     /gene="trfA"
     CDS             complement(3714..4337)
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
     terminator      complement(3585..3671)
                     /note="transcription terminator T1 from the E. coli rr"
     promoter        complement(4356..4411)
                     /gene="tetR"
                     /note="tetR/tetA promoters"
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    complement(4392..4410)
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(4362..4380)
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     misc_feature    5198..7849
                     /note="T7RNApol"
     misc_feature    5108..5191
                     /note="Linker"
     misc_feature    4448..4480
                     /note="T7 tag"
     misc_feature    4481..5101
                     /note="pmCDA1"
     CDS             231..1046
                     /gene="neo"
                     /product="kanamycin resistance protein"
     CDS             complement(1382..2530)
                     /gene="trfA"
                     /note="replication protein"
                     /product="TrfA"
     gene            complement(3714..4337)
                     /gene="tetR"
     gene            4448..7849
                     /gene="pmCDA1-T7RNAP"
     CDS             4448..7849
                     /gene="pmCDA1-T7RNAP"
                     /product="Fusion protein pmCDA1-T7RNAP"
     source          1..7855
                     /dnas_title="pSEVA221_Ptet_pmCDA1_T7RNApol"
ORIGIN      
        1 CTTGGACTCC TGTTGATAGA TCCAGTAATG ACCTCAGAAC TCCATCTGGA TTTGTTCAGA
       61 ACGCTCGGTT GCCGCCGGGC GTTTTTTATT GGTGAGAATC CAGGGGTCCC CAATAATTAC
      121 GATTTAAATT TGTGTCTCAA AATCTCTGAT GTTACATTGC ACAAGATAAA AATATATCAT
      181 CATGAACAAT AAAACTGTCT GCTTACATAA ACAGTAATAC AAGGGGTGTT ATGAGCCATA
      241 TTCAGCGTGA AACGAGCTGT AGCCGTCCGC GTCTGAACAG CAACATGGAT GCGGATCTGT
      301 ATGGCTATAA ATGGGCGCGT GATAACGTGG GTCAGAGCGG CGCGACCATT TATCGTCTGT
      361 ATGGCAAACC GGATGCGCCG GAACTGTTTC TGAAACATGG CAAAGGCAGC GTGGCGAACG
      421 ATGTGACCGA TGAAATGGTG CGTCTGAACT GGCTGACCGA ATTTATGCCG CTGCCGACCA
      481 TTAAACATTT TATTCGCACC CCGGATGATG CGTGGCTGCT GACCACCGCG ATTCCGGGCA
      541 AAACCGCGTT TCAGGTGCTG GAAGAATATC CGGATAGCGG CGAAAACATT GTGGATGCGC
      601 TGGCCGTGTT TCTGCGTCGT CTGCATAGCA TTCCGGTGTG CAACTGCCCG TTTAACAGCG
      661 ATCGTGTGTT TCGTCTGGCC CAGGCGCAGA GCCGTATGAA CAACGGCCTG GTGGATGCGA
      721 GCGATTTTGA TGATGAACGT AACGGCTGGC CGGTGGAACA GGTGTGGAAA GAAATGCATA
      781 AACTGCTGCC GTTTAGCCCG GATAGCGTGG TGACCCACGG CGATTTTAGC CTGGATAACC
      841 TGATTTTCGA TGAAGGCAAA CTGATTGGCT GCATTGATGT GGGCCGTGTG GGCATTGCGG
      901 ATCGTTATCA GGATCTGGCC ATTCTGTGGA ACTGCCTGGG CGAATTTAGC CCGAGCCTGC
      961 AAAAACGTCT GTTTCAGAAA TATGGCATTG ATAATCCGGA TATGAACAAA CTGCAATTTC
     1021 ATCTGATGCT GGATGAATTT TTCTAATAAT TAATTGGACC GCGGTCCGCG CGTTGTCCTT
     1081 TTCCGCTGCA TAACCCTGCT TCGGGGTCAT TATAGCGATT TTTTCGGTAT ATCCATCCTT
     1141 TTTCGCACGA TATACAGGAT TTTGCCAAAG GGTTCGTGTA GACTTTCCTT GGTGTATCCA
     1201 ACGGCGTCAG CCGGGCAGGA TAGGTGAAGT AGGCCCACCC GCGAGCGGGT GTTCCTTCTT
     1261 CACTGTCCCT TATTCGCACC TGGCGGTGCT CAACGGGAAT CCTGCTCTGC GAGGCTGGCC
     1321 GTAGGCCGGC CGCGATGCAG GTGGCTGCTG AACCCCCAGC CGGAACTGAC CCCACAAGGC
     1381 CCTAGCGTTT GCAATGCACC AGGTCATCAT TGACCCAGGC GTGTTCCACC AGGCCGCTGC
     1441 CTCGCAACTC TTCGCAGGCT TCGCCGACCT GCTCGCGCCA CTTCTTCACG CGGGTGGAAT
     1501 CCGATCCGCA CATGAGGCGG AAGGTTTCCA GCTTGAGCGG GTACGGCTCC CGGTGCGAGC
     1561 TGAAATAGTC GAACATCCGT CGGGCCGTCG GCGACAGCTT GCGGTACTTC TCCCATATGA
     1621 ATTTCGTGTA GTGGTCGCCA GCAAACAGCA CGACGATTTC CTCGTCGATC AGGACCTGGC
     1681 AACGGGACGT TTTCTTGCCA CGGTCCAGGA CGCGGAAGCG GTGCAGCAGC GACACCGATT
     1741 CCAGGTGCCC AACGCGGTCG GACGTGAAGC CCATCGCCGT CGCCTGTAGG CGCGACAGGC
     1801 ATTCCTCGGC CTTCGTGTAA TACCGGCCAT TGATCGACCA GCCCAGGTCC TGGCAAAGCT
     1861 CGTAGAACGT GAAGGTGATC GGCTCGCCGA TAGGGGTGCG CTTCGCGTAC TCCAACACCT
     1921 GCTGCCACAC CAGTTCGTCA TCGTCGGCCC GCAGCTCGAC GCCGGTGTAG GTGATCTTCA
     1981 CGTCCTTGTT GACGTGGAAA ATGACCTTGT TTTGCAGCGC CTCGCGCGGG ATTTTCTTGT
     2041 TGCGCGTGGT GAACAGGGCA GAGCGGGCCG TGTCGTTTGG CATCGCTCGC ATCGTGTCCG
     2101 GCCACGGCGC AATATCGAAC AAGGAAAGCT GCATTTCCTT GATCTGCTGC TTCGTGTGTT
     2161 TCAGCAACGC GGCCTGCTTG GCTTCGCTGA CCTGTTTTGC CAGGTCCTCG CCGGCGGTTT
     2221 TTCGCTTCTT GGTCGTCATA GTTCCTCGCG TGTCGATGGT CATCGACTTC GCCAAACCTG
     2281 CCGCCTCCTG TTCGAGACGA CGCGAACGCT CCACGGCGGC CGATGGCGCG GGCAGGGCAG
     2341 GGGGAGCCAG TTGCACGCTG TCGCGCTCGA TCTTGGCCGT AGCTTGCTGG ACTATCGAGC
     2401 CGACGGACTG GAAGGTTTCG CGGGGCGCAC GCATGACGGT GCGGCTTGCG ATGGTTTCGG
     2461 CATCCTCGGC GGAAAACCCC GCGTCGATCA GTTCTTGCCT GTATGCCTTC CGGTCAAACG
     2521 TCCGATTCAT TCACCCTCCT TGCGGGATTG CCCCGGAATT AATTCCCCGG ATCGATCCGT
     2581 CGATCTTGAT CCCCTGCGCC ATCAGATCCT TGGCGGCAAG AAAGCCATCC AGTTTACTTT
     2641 GCAGGGCTTC CCAACCTTAC CAGAGGGCGC CCCAGCTGGC AATTCCGGTT CGCTTGCTGT
     2701 CCATAAAACC GCCCAGTCTA GCTATCGCCA TGTAAGCCCA CTGCAAGCTA CCTGCTTTCT
     2761 CTTTGCGCTT GCGTTTTCCC TTGTCCAGAT AGCCCAGTAG CTGACATTCA TCCGGGGTCA
     2821 GCACCGTTTC TGCGGACTGG CTTTCTACGT GGCTGCCATT TTTGGGGTGA GGCCGTTCGC
     2881 GGCCGAGGGG CGCAGCCCCT GGGGGGATGG GAGGCCCGCG TTAGCGGGCC GGGAGGGTTC
     2941 GAGAAGGGGG GGCACCCCCC TTCGGCGTGC GCGGTCACGC GCACAGGGCG CAGCCCTGGT
     3001 TAAAAACAAG GTTTATAAAT ATTGGTTTAA AAGCAGGTTA AAAGACAGGT TAGCGGTGGC
     3061 CGAAAAACGG GCGGAAACCC TTGCAAATGC TGGATTTTCT GCCTGTGGAC AGCCCCTCAA
     3121 ATGTCAATAG GTGCGCCCCT CATCTGTCAG CACTCTGCCC CTCAAGTGTC AAGGATCGCG
     3181 CCCCTCATCT GTCAGTAGTC GCGCCCCTCA AGTGTCAATA CCGCAGGGCA CTTATCCCCA
     3241 GGCTTGTCCA CATCATCTGT GGGAAACTCG CGTAAAATCA GGCGTTTTCG CCGATTTGCG
     3301 AGGCTGGCCA GCTCCACGTC GCCGGCCGAA ATCGAGCCTG CCCCTCATCT GTCAACGCCG
     3361 CGCCGGGTGA GTCGGCCCCT CAAGTGTCAA CGTCCGCCCC TCATCTGTCA GTGAGGGCCA
     3421 AGTTTTCCGC GAGGTATCCA CAACGCCGGC GGCCCTACAT GGCTCTGCTG TAGTGAGTGG
     3481 GTTGCGCTCC GGCAGCGGTC CTGATCCCCC GCAGAAAAAA AGGATCTCAA GAAGATCCTT
     3541 TGATCTTTTC TACGGCGCGC CgagctcGAA TTCtagggcg gcggatttgt cctactcagg
     3601 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt
     3661 cgttttattt gatgcctcta gcacgcctgc caggaattgg ggatcggAGA TCTttaagac
     3721 ccactttcac atttaagttg tttttctaat ccgcatataa tcaattcaag gccgaataag
     3781 aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa taatggcggc
     3841 atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc ttgatcttcc
     3901 aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc attctctagt
     3961 gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata ctgtttttct
     4021 gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa agcacatcta
     4081 agacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa agggcaaaag
     4141 tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc ccgcttattt
     4201 tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag tttacgggtt
     4261 gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat cactttactt
     4321 ttatctaatc tggacatcat taattcctaa tttttgttga cactctatca ttgatagagt
     4381 tattttacca ctccctatca gtgatagaga aaagtgaaTC TAGAGTCGAC AAGAAGGAGA
     4441 TATACATATG GCAAGCATGA CCGGTGGTCA GCAGATGGGC ACCGATGCAG AATATGTTCG
     4501 TATTCATGAA AAGCTGGACA TCTATACCTT CAAAAAACAG TTCTTCAACA ACAAAAAGAG
     4561 CGTGAGCCAT CGTTGCTATG TTCTGTTTGA ACTGAAACGT CGTGGTGAAC GTCGTGCATG
     4621 TTTTTGGGGT TATGCAGTTA ATAAACCGCA GAGCGGCACC GAACGTGGTA TTCATGCAGA
     4681 AATCTTTAGC ATTCGCAAAG TGGAAGAATA TCTGCGTGAT AATCCGGGTC AGTTTACCAT
     4741 TAATTGGTAT AGCAGCTGGT CACCGTGTGC AGATTGTGCA GAAAAAATTC TGGAATGGTA
     4801 TAACCAAGAA CTGCGTGGTA ATGGTCATAC CCTGAAAATT TGGGCATGTA AACTGTACTA
     4861 CGAAAAGAAT GCCCGTAATC AGATTGGTCT GTGGAATCTG CGCGATAACG GTGTTGGTCT
     4921 GAATGTTATG GTTAGCGAAC ATTATCAGTG CTGCCGCAAA ATCTTTATTC AGAGCAGCCA
     4981 TAATCAGCTG AATGAAAATC GTTGGCTGGA AAAAACCCTG AAACGTGCCG AAAAACGTCG
     5041 TAGCGAACTG AGCATTATGA TTCAGGTTAA AATTCTGCAC ACCACCAAAA GTCCGGCAGT
     5101 TCTGCAGGGT GGTGGTAGCG GTGGTGGTTC AGGTGGTGGC AGTGGCGGTG GAAGTGGTGG
     5161 CGGTTCTGGC GGAGGATCAG GTGGCGGTAG CAAGCTTatg aacacgatta acatcgctaa
     5221 gaacgacttc tctgacatcg aactggctgc tatcccgttc aacactctgg ctgaccatta
     5281 cggtgagcgt ttagctcgcg aacagttggc ccttgagcat gagtcttacg agatgggtga
     5341 agcacgcttc cgcaagatgt ttgagcgtca acttaaagct ggtgaggttg cggataacgc
     5401 tgccgccaag cctctcatca ctaccctact ccctaagatg attgcacgca tcaacgactg
     5461 gtttgaggaa gtgaaagcta agcgcggcaa gcgcccgaca gccttccagt tcctgcaaga
     5521 aatcaagccg gaagccgtag cgtacatcac cattaagacc actctggctt gcctaaccag
     5581 tgctgacaat acaaccgttc aggctgtagc aagcgcaatc ggtcgggcca ttgaggacga
     5641 ggctcgcttc ggtcgtatcc gtgaccttga agctaagcac ttcaagaaaa acgttgagga
     5701 acaactcaac aagcgcgtag ggcacgtcta caagaaagca tttatgcaag ttgtcgaggc
     5761 tgacatgctc tctaagggtc tactcggtgg cgaggcgtgg tcttcgtggc ataaggaaga
     5821 ctctattcat gtaggagtac gctgcatcga gatgctcatt gagtcaaccg gaatggttag
     5881 cttacaccgc caaaatgctg gcgtagtagg tcaagactct gagactatcg aactcgcacc
     5941 tgaatacgct gaggctatcg caacccgtgc aggtgcgctg gctggcatct ctccgatgtt
     6001 ccaaccttgc gtagttcctc ctaagccgtg gactggcatt actggtggtg gctattgggc
     6061 taacggtcgt cgtcctctgg cgctggtgcg tactcacagt aagaaagcac tgatgcgcta
     6121 cgaagacgtt tacatgcctg aggtgtacaa agcgattaac attgcgcaaa acaccgcatg
     6181 gaaaatcaac aagaaagtcc tagcggtcgc caacgtaatc accaagtgga agcattgtcc
     6241 ggtcgaggac atccctgcga ttgagcgtga agaactcccg atgaaaccgg aagacatcga
     6301 catgaatcct gaggctctca ccgcgtggaa acgtgctgcc gctgctgtgt accgcaagga
     6361 caaggctcgc aagtctcgcc gtatcagcct tgagttcatg cttgagcaag ccaataagtt
     6421 tgctaaccat aaggccatct ggttccctta caacatggac tggcgcggtc gtgtttacgc
     6481 tgtgtcaatg ttcaacccgc aaggtaacga tatgaccaaa ggactgctta cgctggcgaa
     6541 aggtaaacca atcggtaagg aaggttacta ctggctgaaa atccacggtg caaactgtgc
     6601 gggtgtcgat aaggttccgt tccctgagcg catcaagttc attgaggaaa accacgagaa
     6661 catcatggct tgcgctaagt ctccactgga gaacacttgg tgggctgagc aagattctcc
     6721 gttctgcttc cttgcgttct gctttgagta cgctggggta cagcaccacg gcctgagcta
     6781 taactgctcc cttccgctgg cgtttgacgg gtcttgctct ggcatccagc acttctccgc
     6841 gatgctccga gatgaggtag gtggtcgcgc ggttaacttg cttcctagtg aaaccgttca
     6901 ggacatctac gggattgttg ctaagaaagt caacgagatt ctacaagcag acgcaatcaa
     6961 tgggaccgat aacgaagtag ttaccgtgac cgatgagaac actggtgaaa tctctgagaa
     7021 agtcaagctg ggcactaagg cactggctgg tcaatggctg gcttacggtg ttactcgcag
     7081 tgtgactaag cgttcagtca tgacgctggc ttacgggtcc aaagagttcg gcttccgtca
     7141 acaagtgctg gaagatacca ttcagccagc tattgattcc ggcaagggtc tgatgttcac
     7201 tcagccgaat caggctgctg gatacatggc taagctgatt tgggaatctg tgagcgtgac
     7261 ggtggtagct gcggttgaag caatgaactg gcttaagtct gctgctaagc tgctggctgc
     7321 tgaggtcaaa gataagaaga ctggagagat tcttcgcaag cgttgcgctg tgcattgggt
     7381 aactcctgat ggtttccctg tgtggcagga atacaagaag cctattcaga cgcgcttgaa
     7441 cctgatgttc ctcggtcagt tccgcttaca gcctaccatt aacaccaaca aagatagcga
     7501 gattgatgca cacaaacagg agtctggtat cgctcctaac tttgtacaca gccaagacgg
     7561 tagccacctt cgtaagactg tagtgtgggc acacgagaag tacggaatcg aatcttttgc
     7621 actgattcac gactccttcg gtaccattcc ggctgacgct gcgaacctgt tcaaagcagt
     7681 gcgcgaaact atggttgaca catatgagtc ttgtgatgta ctggctgatt tctacgacca
     7741 gttcgctgac cagttgcacg agtctcaatt ggacaaaatg ccagcacttc cggctaaagg
     7801 taacttgaac ctccgtgaca tcttagagtc ggacttcgcg ttcgcgtaaA CTAGT
//

