LOCUS       Exported               10052 bp ds-DNA     circular SYN 01-MAR-2021
DEFINITION  T7p-122-1137-proB.
ACCESSION   urn.local...3lc-d2aees1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 2, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10052
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1184..1328
     modified_base   complement(1..16)
                     /label=USER junction
                     /note="USER junction"
     misc_feature    complement(3..15)
                     /label=SfiI
                     /note="SfiI"
                     /note="Keep unique in all constructs, can be used 
                     toexchange antibiotic resistance markers or plasmid 
                     origins"
     promoter        17..211
                     /note="bla promoter"
                     /note="from pUC19"
     CDS             212..1072
                     /label=bla (AmpR)
                     /note="bla (AmpR)"
                     /note="from pUC19"
     misc_feature    459..484
                     /label=MSP366
                     /note="MSP366"
     misc_feature    924..946
                     /label=534
                     /note="534"
     misc_feature    complement(1051..1094)
                     /label=MSP363
                     /note="MSP363"
     misc_feature    1073..1096
                     /label=MSP362
                     /note="MSP362"
     misc_feature    complement(1106..1133)
     terminator      1123..1167
                     /standard_name="rrnb"
                     /label=rrnb terminator
     misc_feature    complement(1150..1183)
                     /label=MSP383
                     /note="MSP383"
     misc_feature    1157..1188
                     /label=MSP384
                     /note="MSP384"
     misc_feature    1168..1328
     primer_bind     1168..1198
                     /standard_name="DB294"
                     /label=DB294
     primer_bind     complement(1168..1183)
                     /standard_name="AB371 primer bind"
                     /label=AB371 primer bind
     primer_bind     complement(1168..1183)
                     /standard_name="DB195"
                     /label=DB195
     misc_feature    1168..1183
                     /standard_name="USER junction"
                     /label=USER junction
     primer_bind     1210..1261
                     /label=AB365
                     /note="AB365"
     promoter        1233..1273
                     /standard_name="ProB"
                     /label=ProB promoter
                     /note="proD (Insulated Promoter - based on rrnB1 P1)"
     misc_feature    complement(1238..1259)
                     /label=MSP489
                     /note="MSP489"
     -35_signal      1239..1244
                     /label=-35
                     /note="-35"
     primer_bind     complement(1245..1296)
                     /label=AB366
                     /note="AB366"
     misc_feature    complement(1252..1287)
                     /label=MSP485-488
                     /note="MSP485-488"
     -10_signal      1262..1267
                     /label=-10
                     /note="-10"
     prim_transcript 1274
                     /label=+1
                     /note="+1"
     primer_bind     complement(1281..1328)
                     /standard_name="AB367"
                     /label=AB367
     misc_feature    complement(1293..1321)
                     /standard_name="TRB304"
                     /label=TRB304
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(1295..1336)
                     /label=MSP382
                     /note="MSP382"
     misc_feature    1306..1332
                     /standard_name="TRB305"
                     /label=TRB305
                     /note="Geneious type: PCR_primer"
     modified_base   1306..1328
     primer_bind     1306..1328
                     /standard_name="DB293"
                     /label=DB293
     misc_feature    1317..1355
                     /label=MSP381
                     /note="MSP381"
     RBS             1340..1352
                     /standard_name="sd7"
                     /label=sd7
     CDS             1353..1802
                     /standard_name="lysozyme"
                     /label=lysozyme
     misc_feature    1460..1485
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1258"
     misc_feature    1460..1485
                     /label=BCD1258
                     /note="BCD1258"
     misc_feature    complement(1782..1802)
                     /label=MSP912
                     /note="MSP912"
     CDS             1803..1832
                     /standard_name="Linker"
                     /label=Linker
     misc_feature    complement(1803..1812)
                     /label=MSP440
                     /note="MSP440"
     CDS             1833..1895
                     /standard_name="SNAP25"
                     /label=SNAP25
     misc_feature    1835..1893
                     /standard_name="GAACGGTTCCTTATGCGACCAAGAGATCGATTCGATCTGCAG 
                     TATCGAGAGAGCAGATA"
                     /label=Geneious type: Editing History Replacement
                     /note="Geneious type: Editing History Replacement"
     CDS             1896..1925
                     /standard_name="Linker"
                     /label=Linker
     misc_feature    1916..1941
                     /label=MSP1136
                     /note="MSP1136"
     CDS             1926..4574
                     /standard_name="T7 RNAP"
                     /label=T7 RNAP
     misc_feature    1926..1950
                     /standard_name="MSP913"
                     /label=MSP913
                     /note="Geneious type: PCR_primer"
     misc_feature    2132
                     /standard_name="C"
                     /label=C
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2180..2206
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1253"
     misc_feature    2180..2206
                     /label=1253
                     /note="1253"
     misc_feature    2233..2252
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1259"
     misc_feature    2233..2252
                     /label=1259
                     /note="1259"
     misc_feature    2362..2395
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD710"
     misc_feature    complement(2552..2574)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="bcd1175"
     misc_feature    2559..2576
                     /standard_name="SeqT7-1"
                     /label=SeqT7-1
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(2562..2591)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1252"
     misc_feature    complement(2562..2591)
                     /label=1252
                     /note="1252"
     misc_feature    2629..2658
                     /label=t7seq2b
                     /note="t7seq2b"
     misc_feature    2713..2741
                     /label=T7seq2
                     /note="T7seq2"
     misc_feature    complement(2876..2907)
                     /label=T7seq1
                     /note="T7seq1"
     misc_feature    2977..2998
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1260"
     misc_feature    2977..2998
                     /label=1260
                     /note="1260"
     misc_feature    3228..3257
                     /label=t7seq2c
                     /note="t7seq2c"
     misc_feature    3239..3273
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="bcd711"
     misc_feature    3308..3326
                     /standard_name="SeqT7-2"
                     /label=SeqT7-2
                     /note="Geneious type: PCR_primer"
     misc_feature    3611..3638
                     /label=T7 seq3
                     /note="T7 seq3"
     misc_feature    3684..3706
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCd1261"
     misc_feature    3684..3706
                     /label=1261
                     /note="1261"
     misc_feature    complement(3684..3706)
                     /label=MSP347
                     /note="MSP347"
     misc_feature    complement(3809..3836)
                     /label=MSP859
                     /note="MSP859"
     misc_feature    complement(3825..3873)
     misc_feature    3825..3851
                     /label=MSP860
                     /note="MSP860"
     misc_feature    3828..3857
                     /label=T7seq3b
                     /note="T7seq3b"
     misc_feature    3891..3911
                     /label=MSP63
                     /note="MSP63"
     misc_feature    3912..3932
                     /label=MSP62
                     /note="MSP62"
     misc_feature    4254..4276
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1262"
     misc_feature    4254..4276
                     /label=1262
                     /note="1262"
     misc_feature    complement(4553..4590)
                     /standard_name="TRB301"
                     /label=TRB301
                     /note="Geneious type: PCR_primer"
     misc_feature    4555..4591
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(4555..4591)
                     /label=MSP346
                     /note="MSP346"
     misc_feature    complement(4555..4591)
                     /label=346
                     /note="346"
     misc_feature    4567..4591
                     /label=MSP20
                     /note="MSP20"
     misc_feature    complement(4567..4591)
                     /label=MSP21
                     /note="MSP21"
     misc_feature    4574..4613
                     /label=MSP364
                     /note="MSP364"
     misc_feature    4574..4613
                     /label=364
                     /note="364"
     CDS             4589..4591
                     /standard_name="III fragment"
                     /label=III fragment
     misc_feature    4592..4621
                     /label=1042
                     /note="1042"
     modified_base   complement(4592..4604)
                     /label=USER junction
                     /note="USER junction"
     terminator      4605..4646
                     /note="tetA/orfL bidirectional terminator"
                     /note="termination of bla transcript is slightly weakerthan
                     opposite direction"
     misc_feature    complement(4647..4653)
                     /label=Nt-BbvCI
                     /note="Nt-BbvCI"
                     /note="nicking endonuclease site that generates overhang"
     modified_base   complement(4654..6793)
                     /label=USER junction
                     /note="USER junction"
     misc_feature    complement(4661..4668)
                     /label=PacI
                     /note="PacI"
                     /note="Keep unique in all constructs, can be used 
                     toexchange antibiotic resistance marker or selection 
                     components"
     CDS             complement(4671..6794)
                     /label=xluxAB
                     /note="xluxAB"
     misc_feature    complement(5309..5337)
                     /label=708
                     /note="708"
     misc_feature    5330..5360
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP14"
     misc_feature    complement(5330..5360)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP15"
     sig_peptide     complement(5652..5711)
                     /note="GGGGS4 flexible linker"
     misc_feature    complement(6091..6122)
                     /label=707
                     /note="707"
     misc_feature    complement(6740..6766)
                     /label=MSP497
                     /note="MSP497"
     misc_feature    complement(6759..6785)
                     /label=MSP498
                     /note="MSP498"
     CDS             complement(6794..8068)
                     /standard_name="III"
                     /label=III
     misc_feature    6794..6823
                     /label=MSP64
                     /note="MSP64"
     misc_feature    complement(6881..6926)
                     /label=modified to remove internal promoter
                     /note="modified to remove internal promoter"
     misc_feature    complement(6905..6938)
                     /label=706
                     /note="706"
     misc_feature    complement(7669..7698)
                     /label=705
                     /note="705"
     misc_feature    complement(8038..8081)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP124"
     misc_feature    complement(8050..8096)
     misc_feature    8069..8101
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP125"
     RBS             complement(8069..8081)
                     /standard_name="RBS"
                     /note="RBS sd8"
     misc_feature    complement(8083..8088)
                     /label=BsgI
                     /note="BsgI"
     misc_feature    8087..8112
     misc_feature    complement(8088..8095)
                     /label=SbfI
                     /note="SbfI"
     terminator      complement(8096..8241)
                     /standard_name="T1 terminator"
                     /label=T1 terminator
     misc_feature    complement(8240..8263)
     misc_feature    complement(8249..8272)
     promoter        complement(8249..8268)
                     /note="T7 promoter"
     modified_base   complement(8271..8285)
                     /label=USER junction
                     /note="USER junction"
     misc_feature    complement(8272..8278)
                     /label=SanDI
                     /note="SanDI"
                     /note="Keep unique in all constructs, can be used 
                     toexchange f1 origin or selection components"
     misc_feature    complement(8286..8292)
                     /label=Nt-BbvCI
                     /note="Nt-BbvCI"
                     /note="nicking endonuclease site that generates overhang"
     terminator      complement(8293..8337)
                     /note="rrnB1 transcriptional terminator"
     modified_base   complement(8338..8352)
                     /label=USER junction
                     /note="USER junction"
     misc_feature    8351..8377
                     /label=1044
                     /note="1044"
     rep_origin      8353..9098
     misc_feature    complement(8459..8485)
                     /label=535
                     /note="535"
     misc_feature    9083..9126
                     /label=814
                     /note="814"
     CDS             9102..10052
                     /label=RepA
                     /note="RepA"
                     /note="pSC101 origin of replication protein"
     misc_feature    9853..9876
                     /label=MSP365
                     /note="MSP365"
ORIGIN
        1 acggcctatt tggcctaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
       61 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      121 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      181 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      241 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      301 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      361 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      421 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      481 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      541 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      601 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      661 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
      721 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
      781 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
      841 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
      901 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
      961 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1021 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aacgtaatgc
     1081 tctgccagtg ttacaaccaa ttaaccaatt ctgatttccg gtcaaataaa acgaaaggct
     1141 cagtcgaaag actgggcctt tcgttttatt ctagagcaca gctaacacca cgtcgtccct
     1201 atctgctgcc ctaggtctat gagtggttgc tggataactt tacgggcatg cataaggctc
     1261 gtaatatata ttcagggaga ccacaacggt ttccctctac aaataatttt gtttaacttt
     1321 tactagagtt ccttcctcga aaggaaaaaa aaatggctcg tgtacagttt aaacaacgtg
     1381 aatctactga cgcaatcttt gttcactgct cggctaccaa gccaagtcag aatgttggtg
     1441 tccgtgagat tcgccagtgg cacaaagagc agggttggct cgatgtggga taccacttta
     1501 tcatcaagcg agacggtact gtggaggcag gacgagatga gatggctgta ggctctcacg
     1561 ctaagggtta caaccacaac tctatcggcg tctgccttgt tggtggtatc gacgataaag
     1621 gtaagttcga cgctaacttt acgccagccc aaatgcaatc ccttcgctca ctgcttgtca
     1681 cactgctggc taagtacgaa ggcgctgtgc ttcgcgccca tcatgaggtg gcgccgaagg
     1741 cttccccttc gttcgacctt aagcgttggt gggagaagaa cgaactggtc acatctgacc
     1801 gtggtagcgg cggtggtgcg agtggtggtg cggacatggg taacgaaatc gacacccaga
     1861 accgtcagat cgaccgtatc atggaaaaag ctgacggcgg tagcgcaggc agtggagcgg
     1921 gcggtaacac gattaacatc gctaagaacg acttctctga catcgaactg gctgctatcc
     1981 cgttcaacac tctggctgac cattacggtg agcgtttagc tcgcgaacag ttggcccttg
     2041 agcatgagtc ttacgagatg ggtgaagcac gcttccgcaa gatgtttgag cgtcaactta
     2101 aagctggtga ggttgcggat aacgctgccg ctaagcctct catcactacc ctactcccta
     2161 agatgattgc acgcatcaac gactggtttg aggaagtgaa agctaagcgc ggcaagcgcc
     2221 cgacagcctt ccagttcctg caagaaatca agccggaagc cgtagcgtac atcaccatta
     2281 agaccactct ggcttgccta accagtgctg acaatacaac cgttcaggct gtagcaagcg
     2341 caatcggtcg ggccattgag gacgaggctc gcttcggtcg tatccgtgac cttgaagcta
     2401 agcacttcaa gaaaaacgtt gaggaacaac tcaacaagcg cgtagggcac gtctacaaga
     2461 aagcatttat gcaagttgtc gaggctgaca tgctctctaa gggtctactc ggtggcgagg
     2521 cgtggtcttc gtggcataag gaagactcta ttcatgtagg agtacgctgc atcgagatgc
     2581 tcattgagtc aaccggaatg gttagcttac accgccaaaa tgctggcgta gtaggtcaag
     2641 actctgagac tatcgaactc gcacctgaat acgctgaggc tatcgcaacc cgtgcaggtg
     2701 cgctggctgg catctctccg atgttccaac cttgcgtagt tcctcctaag ccgtggactg
     2761 gcattactgg tggtggctat tgggctaacg gtcgtcgtcc tctggcgctg gtgcgtactc
     2821 acagtaagaa agcactgatg cgctacgaag acgtttacat gcctgaggtg tacaaagcga
     2881 ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa agtcctagcg gtcgccaacg
     2941 taatcaccaa gtggaagcat tgtccggtcg aggacatccc tgcgattgag cgtgaagaac
     3001 tcccgatgaa accggaagac atcgacatga atcctgaggc tctcaccgcg tggaaacgtg
     3061 ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc tcgccgtatc agccttgagt
     3121 tcatgcttga gcaagccaat aagtttgcta accataaggc catctggttc ccttacaaca
     3181 tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa cccgcaaggt aacgatatga
     3241 ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg taaggaaggt tactactggc
     3301 tgaaaatcca cggtgcaaac tgtgcgggtg tcgataaggt tccgttccct gagcgcatca
     3361 agttcattga ggaaaaccac gagaacatca tggcttgcgc taagtctcca ctggagaaca
     3421 cttggtgggc tgagcaagat tctccgttct gcttccttgc gttctgcttt gagtacgctg
     3481 gggtacagca ccacggcctg agctataact gctcccttcc gctggcgttt gacgggtctt
     3541 gctctggcat ccagcacttc tccgcgatgc tccgagatga ggtaggtggt cgcgcggtta
     3601 acttgcttcc tagtgaaacc gttcaggaca tctacgggat tgttgctaag aaagtcaacg
     3661 agattctaca agcagacgca atcaatggga ccgataacga agtagttacc gtgaccgatg
     3721 agaacactgg tgaaatctct gagaaagtca agctgggcac taaggcactg gctggtcaat
     3781 ggctggctta cggtgttact cgcagtgtga ctaagcgttc agtcatgacg ctggcttacg
     3841 ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga taccattcag ccagctattg
     3901 attccggcaa gggtctgatg ttcactcagc cgaatcaggc tgctggatac atggctaagc
     3961 tgatttggga atctgtgagc gtgacggtgg tagctgcggt tgaagcaatg aactggctta
     4021 agtctgctgc taagctgctg gctgctgagg tcaaagataa gaagactgga gagattcttc
     4081 gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt ccctgtgtgg caggaataca
     4141 agaagcctat tcagacgcgc ttgaacctga tgttcctcgg tcagttccgc ttacagccta
     4201 ccattaacac caacaaagat agcgagattg atgcacacaa acaggagtct ggtatcgctc
     4261 ctaactttgt acacagccaa gacggtagcc accttcgtaa gactgtagtg tgggcacacg
     4321 agaagtacgg aatcgaatct tttgcactga ttcacgactc cttcggtacc attccggctg
     4381 acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt tgacacatat gagtcttgtg
     4441 atgtactggc tgatttctac gaccagttcg ctgaccagtt gcacgagtct caattggaca
     4501 aaatgccagc acttccggct aaaggtaact tgaacctccg tgacatctta gagtcggact
     4561 tcgcgttcgc gtaatggaga ttttcaacat gacgccatgg gcattaaaaa atgccctctt
     4621 gggttatcaa gagggtcatt atatttgctg aggagtgccg ttaattaagt ttaggtatat
     4681 tccgtgtggt acttcttaat attatcatca acaatattga ttacattttt ttggctcatc
     4741 aaatcattca ttggttcaaa ggacagcaat acacttttcg caccacactt ttcaattgcc
     4801 aacttagccg cagttataca ctccgtataa tttccgacag cgttttctgc aattatttct
     4861 tcaagtttat tttcgaaatt ttcattaggg tgcatttcaa gaacataatc actaataaat
     4921 gcacgcgtct cttgtttagc tttattacta tcttcgttat agttaactaa tatcattaac
     4981 tgatggtcta tctctgatag gtcaacgtca tatttatccg caacggcttt atatctttca
     5041 gcatattcat atctaacatc attagaatca tcccacttaa agatgagagg aatacctttt
     5101 ttggccgccc actcaacaat atgatgactg gttgctgtta catatttccg aggtccgcct
     5161 ggcgtataag catggggatt tacagatatt ttagggaagc tataaaaatc gttatctgga
     5221 ttacaatagc ctgttgttaa agcatcgtta atgatttcat aacactcttc aaatagttgc
     5281 tgttgatatt caaccgggcg attaaaaaaa tgcatttcat cttttttttc gcaatcacta
     5341 aaccctaaaa taaatctccc ttcacttaac tgatccaata agcaagcttc ctccgctatg
     5401 cggacaggat gatgagttgt aatgatgtga tttaatgaac caattttaat tttctctgtt
     5461 aaaccgagca gaaaaccaga aacagtcaga ggagcgccga caacaccatt atctgaaaaa
     5521 tgattttcat acactaaaat ctgttcaaaa ttcaacttat caacatactc cgttatttcc
     5581 tgcatgcgaa ctatactctg ttcttgaaca gttgttgaat tgatgaagtt aaggaagaac
     5641 aatccaaatt tgctaccgcc accgccgcta ccgccaccgc cgctaccgcc accgccgcta
     5701 ccgccaccgc cataatataa tagcgaacgt tgtttttctt taagaaatgg catgacatca
     5761 gactggaaga gcttcatgga agcaataatt tcgtctactg ttccattagc ttcaaatcca
     5821 caacaaatat ttgatattcc tgtagcatca atgtcttttt gaattatgtc aatacattcc
     5881 tgcggcgttc ccacgggatt gatttcgtaa ctgtaatcaa tacggcgatt agtatcttta
     5941 tgtcctttta atacaaagtc acgccactgc cctttattga aatcataacc tcttgtttgg
     6001 tctgaatcat caaaaatagt cgtagcattc acataagaat cataccaatg ccccagaaat
     6061 ttccggcaaa tctctttcgc tttaattgag tcatgatcta cagatgttat atatgataag
     6121 caatggtcga tattatgaat atcgtgccca tattcttgag ccacttcatt ataaagctca
     6181 agttgtgctt tcttttcgtt agtatttata atccaactta atatcatcgg taggccaaat
     6241 tgagcagccc actcagtcgt cgaagctgat tcagccacca cataaaccgg tgcgccacct
     6301 ctgctatacg ccgcggggtt tacttttacc ttatggaact tgatatgttc attatcagct
     6361 tccatatatc cctctgtcat gccattcttt atcagcccgt accagcattc cgctaaggcg
     6421 cgactgttat tcatatctgt gccgaatacg cgaaagtcct tgttgtaaag ccctcggcaa
     6481 ataccaaacc gaaatcgtcc ttttgacatt tgatccaata aattcacatc ttcaagttgg
     6541 cgtactggat gggctgtggg aagaacaata gcggcagttc ctacattcaa ttttttagtc
     6601 gcgccaagta aatatgcagc agcgacataa gggttaccaa gcaaaccaaa ctccgtgaaa
     6661 tgatgctcca gtaaccatac ggtatcaaaa ccacactcct cagagatgcg acctaattta
     6721 accaaacgtt tcattacctc tgtttgggaa aattggggag gttggtatgt aagcaaaaag
     6781 tttccaaatt tcattaagac tccttattac gcagtatgtt agcaaacgta gaaaatacat
     6841 acataaaggt ggcaacatat aaaagaaacg caaagacacc gcggaacagg ttgatcttat
     6901 cgcagtcgat actgaactcg taaggtttac cagcgccaaa gacaaaaggg cgacattcaa
     6961 ccgattgagg gagggaaggt aaatattgac ggaaattatt cattaaaggt gaattatcac
     7021 cgtcaccgac ttgagccatt tgggaattag agccagcaaa atcaccagta gcaccattac
     7081 cattagcaag gccggaaacg tcaccaatga aaccatcgat agcagcaccg taatcagtag
     7141 cgacagaatc aagtttgcct ttagcgtcag actgtagcgc gttttcatcg gcattttcgg
     7201 tcatagcccc cttattagcg tttgccatct tttcataatc aaaatcaccg gaaccagagc
     7261 caccaccgga accgcctccc tcagagccgc caccctcaga accgccaccc tcagagccac
     7321 caccctcaga gccgccacca gaaccaccac cagagccgcc gccagcattg acaggaggtt
     7381 gaggcaggtc agacgattgg ccttgatatt cacaaacgaa tggatcctca ttaaagccag
     7441 aatggaaagc gcagtctctg aatttaccgt tccagtaagc gtcatacatg gcttttgatg
     7501 atacaggagt gtactggtaa taagttttaa cggggtcagt gccttgagta acagtgcccg
     7561 tataaacagt taatgccccc tgcctatttc ggaacctatt attctgaaac atgaaagtat
     7621 taagaggctg agactcctca agagaaggat taggattagc ggggttttgc tcagtaccag
     7681 gcggataagt gccgtcgaga gggttgatat aagtatagcc cggaataggt gtatcaccgt
     7741 actcaggagg tttagtaccg ccaccctcag aaccgccacc ctcagaaccg ccaccctcag
     7801 agccaccacc ctcattttca gggatagcaa gcccaatagg aacccatgta ccgtaacact
     7861 gagtttcgtc accagtacaa actacaacgc ctgtagcatt ccacagacag ccctcatagt
     7921 tagcgtaacg atctaaagtt ttgtcgtctt tccagacgtt agtaaatgaa ttttctgtat
     7981 ggggttttgc taaacaactt tcaacagttt cagcggagtg agaatagaaa ggaacaacta
     8041 aaggaattgc gaataataat tttttcattt ttttttcctt tactgcacct gcaggtcttt
     8101 cttatgagtt acctcctgaa cttggtaaac agaacaacaa agctgcctga tagtggcctt
     8161 tatttttggc ataaataaca gaataaacac tgcactgtgt attcattcca acgagtgaat
     8221 acacggagca atgtcgctcc ttctttctcc ctatagtgag tcgtattagt agggtcccta
     8281 agtctcctca gcaaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgacc
     8341 ggatgtcctc ttgacagtaa gacgggtaag cctgttgatg ataccgctgc cttactgggt
     8401 gcattagcca gtctgaatga cctgtcacgg gataatccga agtggtcaga ctggaaaatc
     8461 agagggcagg aactgctgaa cagcaaaaag tcagatagca ccacatagca gacccgccat
     8521 aaaacgccct gagaagcccg tgacgggctt ttcttgtatt atgggtagtt tccttgcatg
     8581 aatccataaa aggcgcctgt agtgccattt acccccattc actgccagag ccgtgagcgc
     8641 agcgaactga atgtcacgaa aaagacagcg actcaggtgc ctgatggtcg gagacaaaag
     8701 gaatattcag cgatttgccc gagcttgcga gggtgctact taagccttta gggttttaag
     8761 gtctgttttg tagaggagca aacagcgttt gcgacatcct tttgtaatac tgcggaactg
     8821 actaaagtag tgagttatac acagggctgg gatctattct ttttatcttt ttttattctt
     8881 tctttattct ataaattata accacttgaa tataaacaaa aaaaacacac aaaggtctag
     8941 cggaatttac agagggtcta gcagaattta caagttttcc agcaaaggtc tagcagaatt
     9001 tacagatacc cacaactcaa aggaaaagga ctagtaatta tcattgacta gcccatctca
     9061 attggtatag tgattaaaat cacctagacc aattgagatg tatgtctgaa ttagttgttt
     9121 tcaaagcaaa tgaactagcg attagtcgct atgacttaac ggagcatgaa accaagctaa
     9181 ttttatgctg tgtggcacta ctcaacccca cgattgaaaa ccctacaagg aaagaacgga
     9241 cggtatcgtt cacttataac caatacgctc agatgatgaa catcagtagg gaaaatgctt
     9301 atggtgtatt agctaaagca accagagagc tgatgacgag aactgtggaa atcaggaatc
     9361 ctttggttaa aggctttgag attttccagt ggacaaacta tgccaagttc tcaagcgaaa
     9421 aattagaatt agtttttagt gaagagatat tgccttatct tttccagtta aaaaaattca
     9481 taaaatataa tctggaacat gttaagtctt ttgaaaacaa atactctatg aggatttatg
     9541 agtggttatt aaaagaacta acacaaaaga aaactcacaa ggcaaatata gagattagcc
     9601 ttgatgaatt taagttcatg ttaatgcttg aaaataacta ccatgagttt aaaaggctta
     9661 accaatgggt tttgaaacca ataagtaaag atttaaacac ttacagcaat atgaaattgg
     9721 tggttgataa gcgaggccgc ccgactgata cgttgatttt ccaagttgaa ctagatagac
     9781 aaatggatct cgtaaccgaa cttgagaaca accagataaa aatgaatggt gacaaaatac
     9841 caacaaccat tacatcagat tcctacctac gtaacggact aagaaaaaca ctacacgatg
     9901 ctttaactgc aaaaattcag ctcaccagtt ttgaggcaaa atttttgagt gacatgcaaa
     9961 gtaagcatga tctcaatggt tcgttctcat ggctcacgca aaaacaacga accacactag
    10021 agaacatact ggctaaatac ggaaggatct ga
//
