LOCUS       Exported               10040 bp ds-DNA     circular SYN 01-MAR-2021
DEFINITION  T7p-122-608-proB.
ACCESSION   urn.local...3mi-d2aqzkw
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 2, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10040
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1184..1328
     modified_base   complement(1..16)
                     /label=USER junction
                     /note="USER junction"
     misc_feature    complement(3..15)
                     /label=SfiI
                     /note="SfiI"
                     /note="Keep unique in all constructs, can be used 
                     toexchange antibiotic resistance markers or plasmid 
                     origins"
     promoter        17..211
                     /note="bla promoter"
                     /note="from pUC19"
     CDS             212..1072
                     /label=bla (AmpR)
                     /note="bla (AmpR)"
                     /note="from pUC19"
     misc_feature    459..484
                     /label=MSP366
                     /note="MSP366"
     misc_feature    924..946
                     /label=534
                     /note="534"
     misc_feature    complement(1051..1094)
                     /label=MSP363
                     /note="MSP363"
     misc_feature    1073..1096
                     /label=MSP362
                     /note="MSP362"
     misc_feature    complement(1106..1133)
     terminator      1123..1167
                     /standard_name="rrnb"
                     /label=rrnb terminator
     misc_feature    complement(1150..1183)
                     /label=MSP383
                     /note="MSP383"
     misc_feature    1157..1188
                     /label=MSP384
                     /note="MSP384"
     misc_feature    1168..1328
     primer_bind     1168..1198
                     /standard_name="DB294"
                     /label=DB294
     primer_bind     complement(1168..1183)
                     /standard_name="AB371 primer bind"
                     /label=AB371 primer bind
     primer_bind     complement(1168..1183)
                     /standard_name="DB195"
                     /label=DB195
     misc_feature    1168..1183
                     /standard_name="USER junction"
                     /label=USER junction
     primer_bind     1210..1261
                     /label=AB365
                     /note="AB365"
     promoter        1233..1273
                     /standard_name="ProB"
                     /label=ProB promoter
                     /note="proD (Insulated Promoter - based on rrnB1 P1)"
     misc_feature    complement(1238..1259)
                     /label=MSP489
                     /note="MSP489"
     -35_signal      1239..1244
                     /label=-35
                     /note="-35"
     primer_bind     complement(1245..1296)
                     /label=AB366
                     /note="AB366"
     misc_feature    complement(1252..1287)
                     /label=MSP485-488
                     /note="MSP485-488"
     -10_signal      1262..1267
                     /label=-10
                     /note="-10"
     prim_transcript 1274
                     /label=+1
                     /note="+1"
     primer_bind     complement(1281..1328)
                     /standard_name="AB367"
                     /label=AB367
     misc_feature    complement(1293..1321)
                     /standard_name="TRB304"
                     /label=TRB304
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(1295..1336)
                     /label=MSP382
                     /note="MSP382"
     misc_feature    1306..1332
                     /standard_name="TRB305"
                     /label=TRB305
                     /note="Geneious type: PCR_primer"
     modified_base   1306..1328
     primer_bind     1306..1328
                     /standard_name="DB293"
                     /label=DB293
     misc_feature    1317..1355
                     /label=MSP381
                     /note="MSP381"
     RBS             1340..1352
                     /standard_name="sd7"
                     /label=sd7
     CDS             1353..1802
                     /standard_name="lysozyme"
                     /label=lysozyme
     misc_feature    1460..1485
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1258"
     misc_feature    1460..1485
                     /label=BCD1258
                     /note="BCD1258"
     misc_feature    complement(1782..1802)
                     /label=MSP912
                     /note="MSP912"
     CDS             1803..1832
                     /standard_name="Linker"
                     /label=Linker
     misc_feature    complement(1810..1829)
                     /label=MSP476
                     /note="MSP476"
     misc_feature    1833..1883
                     /standard_name="GAGAACGGTTCCTTATGCGACCAAGAGATCGATTCGATCTGC 
                     AGTATCGAGAGAGCAGATAAC"
                     /label=Geneious type: Editing History Replacement
                     /note="Geneious type: Editing History Replacement"
     CDS             1833..1883
                     /standard_name="VAMP4"
                     /label=VAMP4
     CDS             1884..1913
                     /standard_name="Linker"
                     /label=Linker
     CDS             1914..4562
                     /standard_name="T7 RNAP"
                     /label=T7 RNAP
     misc_feature    1914..1938
                     /standard_name="MSP913"
                     /label=MSP913
                     /note="Geneious type: PCR_primer"
     misc_feature    1914..1929
                     /label=MSP1136
                     /note="MSP1136"
     misc_feature    2120
                     /standard_name="C"
                     /label=C
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2168..2194
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1253"
     misc_feature    2168..2194
                     /label=1253
                     /note="1253"
     misc_feature    2221..2240
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1259"
     misc_feature    2221..2240
                     /label=1259
                     /note="1259"
     misc_feature    2350..2383
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD710"
     misc_feature    complement(2540..2562)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="bcd1175"
     misc_feature    2547..2564
                     /standard_name="SeqT7-1"
                     /label=SeqT7-1
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(2550..2579)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1252"
     misc_feature    complement(2550..2579)
                     /label=1252
                     /note="1252"
     misc_feature    2617..2646
                     /label=t7seq2b
                     /note="t7seq2b"
     misc_feature    2701..2729
                     /label=T7seq2
                     /note="T7seq2"
     misc_feature    complement(2864..2895)
                     /label=T7seq1
                     /note="T7seq1"
     misc_feature    2965..2986
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1260"
     misc_feature    2965..2986
                     /label=1260
                     /note="1260"
     misc_feature    3216..3245
                     /label=t7seq2c
                     /note="t7seq2c"
     misc_feature    3227..3261
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="bcd711"
     misc_feature    3296..3314
                     /standard_name="SeqT7-2"
                     /label=SeqT7-2
                     /note="Geneious type: PCR_primer"
     misc_feature    3599..3626
                     /label=T7 seq3
                     /note="T7 seq3"
     misc_feature    3672..3694
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCd1261"
     misc_feature    3672..3694
                     /label=1261
                     /note="1261"
     misc_feature    complement(3672..3694)
                     /label=MSP347
                     /note="MSP347"
     misc_feature    complement(3797..3824)
                     /label=MSP859
                     /note="MSP859"
     misc_feature    complement(3813..3861)
     misc_feature    3813..3839
                     /label=MSP860
                     /note="MSP860"
     misc_feature    3816..3845
                     /label=T7seq3b
                     /note="T7seq3b"
     misc_feature    3879..3899
                     /label=MSP63
                     /note="MSP63"
     misc_feature    3900..3920
                     /label=MSP62
                     /note="MSP62"
     misc_feature    4242..4264
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1262"
     misc_feature    4242..4264
                     /label=1262
                     /note="1262"
     misc_feature    complement(4541..4578)
                     /standard_name="TRB301"
                     /label=TRB301
                     /note="Geneious type: PCR_primer"
     misc_feature    4543..4579
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(4543..4579)
                     /label=MSP346
                     /note="MSP346"
     misc_feature    complement(4543..4579)
                     /label=346
                     /note="346"
     misc_feature    4555..4579
                     /label=MSP20
                     /note="MSP20"
     misc_feature    complement(4555..4579)
                     /label=MSP21
                     /note="MSP21"
     misc_feature    4562..4601
                     /label=MSP364
                     /note="MSP364"
     misc_feature    4562..4601
                     /label=364
                     /note="364"
     CDS             4577..4579
                     /standard_name="III fragment"
                     /label=III fragment
     misc_feature    4580..4609
                     /label=1042
                     /note="1042"
     modified_base   complement(4580..4592)
                     /label=USER junction
                     /note="USER junction"
     terminator      4593..4634
                     /note="tetA/orfL bidirectional terminator"
                     /note="termination of bla transcript is slightly weakerthan
                     opposite direction"
     misc_feature    complement(4635..4641)
                     /label=Nt-BbvCI
                     /note="Nt-BbvCI"
                     /note="nicking endonuclease site that generates overhang"
     modified_base   complement(4642..6781)
                     /label=USER junction
                     /note="USER junction"
     misc_feature    complement(4649..4656)
                     /label=PacI
                     /note="PacI"
                     /note="Keep unique in all constructs, can be used 
                     toexchange antibiotic resistance marker or selection 
                     components"
     CDS             complement(4659..6782)
                     /label=xluxAB
                     /note="xluxAB"
     misc_feature    complement(5297..5325)
                     /label=708
                     /note="708"
     misc_feature    5318..5348
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP14"
     misc_feature    complement(5318..5348)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP15"
     sig_peptide     complement(5640..5699)
                     /note="GGGGS4 flexible linker"
     misc_feature    complement(6079..6110)
                     /label=707
                     /note="707"
     misc_feature    complement(6728..6754)
                     /label=MSP497
                     /note="MSP497"
     misc_feature    complement(6747..6773)
                     /label=MSP498
                     /note="MSP498"
     CDS             complement(6782..8056)
                     /standard_name="III"
                     /label=III
     misc_feature    6782..6811
                     /label=MSP64
                     /note="MSP64"
     misc_feature    complement(6869..6914)
                     /label=modified to remove internal promoter
                     /note="modified to remove internal promoter"
     misc_feature    complement(6893..6926)
                     /label=706
                     /note="706"
     misc_feature    complement(7657..7686)
                     /label=705
                     /note="705"
     misc_feature    complement(8026..8069)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP124"
     misc_feature    complement(8038..8084)
     misc_feature    8057..8089
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP125"
     RBS             complement(8057..8069)
                     /standard_name="RBS"
                     /note="RBS sd8"
     misc_feature    complement(8071..8076)
                     /label=BsgI
                     /note="BsgI"
     misc_feature    8075..8100
     misc_feature    complement(8076..8083)
                     /label=SbfI
                     /note="SbfI"
     terminator      complement(8084..8229)
                     /standard_name="T1 terminator"
                     /label=T1 terminator
     misc_feature    complement(8228..8251)
     misc_feature    complement(8237..8260)
     promoter        complement(8237..8256)
                     /note="T7 promoter"
     modified_base   complement(8259..8273)
                     /label=USER junction
                     /note="USER junction"
     misc_feature    complement(8260..8266)
                     /label=SanDI
                     /note="SanDI"
                     /note="Keep unique in all constructs, can be used 
                     toexchange f1 origin or selection components"
     misc_feature    complement(8274..8280)
                     /label=Nt-BbvCI
                     /note="Nt-BbvCI"
                     /note="nicking endonuclease site that generates overhang"
     terminator      complement(8281..8325)
                     /note="rrnB1 transcriptional terminator"
     modified_base   complement(8326..8340)
                     /label=USER junction
                     /note="USER junction"
     misc_feature    8339..8365
                     /label=1044
                     /note="1044"
     rep_origin      8341..9086
     misc_feature    complement(8447..8473)
                     /label=535
                     /note="535"
     misc_feature    9071..9114
                     /label=814
                     /note="814"
     CDS             9090..10040
                     /label=RepA
                     /note="RepA"
                     /note="pSC101 origin of replication protein"
     misc_feature    9841..9864
                     /label=MSP365
                     /note="MSP365"
ORIGIN
        1 acggcctatt tggcctaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
       61 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      121 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      181 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      241 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      301 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      361 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      421 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      481 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      541 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      601 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      661 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
      721 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
      781 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
      841 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
      901 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
      961 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1021 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aacgtaatgc
     1081 tctgccagtg ttacaaccaa ttaaccaatt ctgatttccg gtcaaataaa acgaaaggct
     1141 cagtcgaaag actgggcctt tcgttttatt ctagagcaca gctaacacca cgtcgtccct
     1201 atctgctgcc ctaggtctat gagtggttgc tggataactt tacgggcatg cataaggctc
     1261 gtaatatata ttcagggaga ccacaacggt ttccctctac aaataatttt gtttaacttt
     1321 tactagagtt ccttcctcga aaggaaaaaa aaatggctcg tgtacagttt aaacaacgtg
     1381 aatctactga cgcaatcttt gttcactgct cggctaccaa gccaagtcag aatgttggtg
     1441 tccgtgagat tcgccagtgg cacaaagagc agggttggct cgatgtggga taccacttta
     1501 tcatcaagcg agacggtact gtggaggcag gacgagatga gatggctgta ggctctcacg
     1561 ctaagggtta caaccacaac tctatcggcg tctgccttgt tggtggtatc gacgataaag
     1621 gtaagttcga cgctaacttt acgccagccc aaatgcaatc ccttcgctca ctgcttgtca
     1681 cactgctggc taagtacgaa ggcgctgtgc ttcgcgccca tcatgaggtg gcgccgaagg
     1741 cttccccttc gttcgacctt aagcgttggt gggagaagaa cgaactggtc acatctgacc
     1801 gtggtagcgg cggtggtgcg agtggtggtg cgattgagcg tggagaacgc cttgacgaat
     1861 tgcaagacaa atcggagtct cttggcggta gcgcaggcag tggagcgggc ggtaacacga
     1921 ttaacatcgc taagaacgac ttctctgaca tcgaactggc tgctatcccg ttcaacactc
     1981 tggctgacca ttacggtgag cgtttagctc gcgaacagtt ggcccttgag catgagtctt
     2041 acgagatggg tgaagcacgc ttccgcaaga tgtttgagcg tcaacttaaa gctggtgagg
     2101 ttgcggataa cgctgccgct aagcctctca tcactaccct actccctaag atgattgcac
     2161 gcatcaacga ctggtttgag gaagtgaaag ctaagcgcgg caagcgcccg acagccttcc
     2221 agttcctgca agaaatcaag ccggaagccg tagcgtacat caccattaag accactctgg
     2281 cttgcctaac cagtgctgac aatacaaccg ttcaggctgt agcaagcgca atcggtcggg
     2341 ccattgagga cgaggctcgc ttcggtcgta tccgtgacct tgaagctaag cacttcaaga
     2401 aaaacgttga ggaacaactc aacaagcgcg tagggcacgt ctacaagaaa gcatttatgc
     2461 aagttgtcga ggctgacatg ctctctaagg gtctactcgg tggcgaggcg tggtcttcgt
     2521 ggcataagga agactctatt catgtaggag tacgctgcat cgagatgctc attgagtcaa
     2581 ccggaatggt tagcttacac cgccaaaatg ctggcgtagt aggtcaagac tctgagacta
     2641 tcgaactcgc acctgaatac gctgaggcta tcgcaacccg tgcaggtgcg ctggctggca
     2701 tctctccgat gttccaacct tgcgtagttc ctcctaagcc gtggactggc attactggtg
     2761 gtggctattg ggctaacggt cgtcgtcctc tggcgctggt gcgtactcac agtaagaaag
     2821 cactgatgcg ctacgaagac gtttacatgc ctgaggtgta caaagcgatt aacattgcgc
     2881 aaaacaccgc atggaaaatc aacaagaaag tcctagcggt cgccaacgta atcaccaagt
     2941 ggaagcattg tccggtcgag gacatccctg cgattgagcg tgaagaactc ccgatgaaac
     3001 cggaagacat cgacatgaat cctgaggctc tcaccgcgtg gaaacgtgct gccgctgctg
     3061 tgtaccgcaa ggacaaggct cgcaagtctc gccgtatcag ccttgagttc atgcttgagc
     3121 aagccaataa gtttgctaac cataaggcca tctggttccc ttacaacatg gactggcgcg
     3181 gtcgtgttta cgctgtgtca atgttcaacc cgcaaggtaa cgatatgacc aaaggactgc
     3241 ttacgctggc gaaaggtaaa ccaatcggta aggaaggtta ctactggctg aaaatccacg
     3301 gtgcaaactg tgcgggtgtc gataaggttc cgttccctga gcgcatcaag ttcattgagg
     3361 aaaaccacga gaacatcatg gcttgcgcta agtctccact ggagaacact tggtgggctg
     3421 agcaagattc tccgttctgc ttccttgcgt tctgctttga gtacgctggg gtacagcacc
     3481 acggcctgag ctataactgc tcccttccgc tggcgtttga cgggtcttgc tctggcatcc
     3541 agcacttctc cgcgatgctc cgagatgagg taggtggtcg cgcggttaac ttgcttccta
     3601 gtgaaaccgt tcaggacatc tacgggattg ttgctaagaa agtcaacgag attctacaag
     3661 cagacgcaat caatgggacc gataacgaag tagttaccgt gaccgatgag aacactggtg
     3721 aaatctctga gaaagtcaag ctgggcacta aggcactggc tggtcaatgg ctggcttacg
     3781 gtgttactcg cagtgtgact aagcgttcag tcatgacgct ggcttacggg tccaaagagt
     3841 tcggcttccg tcaacaagtg ctggaagata ccattcagcc agctattgat tccggcaagg
     3901 gtctgatgtt cactcagccg aatcaggctg ctggatacat ggctaagctg atttgggaat
     3961 ctgtgagcgt gacggtggta gctgcggttg aagcaatgaa ctggcttaag tctgctgcta
     4021 agctgctggc tgctgaggtc aaagataaga agactggaga gattcttcgc aagcgttgcg
     4081 ctgtgcattg ggtaactcct gatggtttcc ctgtgtggca ggaatacaag aagcctattc
     4141 agacgcgctt gaacctgatg ttcctcggtc agttccgctt acagcctacc attaacacca
     4201 acaaagatag cgagattgat gcacacaaac aggagtctgg tatcgctcct aactttgtac
     4261 acagccaaga cggtagccac cttcgtaaga ctgtagtgtg ggcacacgag aagtacggaa
     4321 tcgaatcttt tgcactgatt cacgactcct tcggtaccat tccggctgac gctgcgaacc
     4381 tgttcaaagc agtgcgcgaa actatggttg acacatatga gtcttgtgat gtactggctg
     4441 atttctacga ccagttcgct gaccagttgc acgagtctca attggacaaa atgccagcac
     4501 ttccggctaa aggtaacttg aacctccgtg acatcttaga gtcggacttc gcgttcgcgt
     4561 aatggagatt ttcaacatga cgccatgggc attaaaaaat gccctcttgg gttatcaaga
     4621 gggtcattat atttgctgag gagtgccgtt aattaagttt aggtatattc cgtgtggtac
     4681 ttcttaatat tatcatcaac aatattgatt acattttttt ggctcatcaa atcattcatt
     4741 ggttcaaagg acagcaatac acttttcgca ccacactttt caattgccaa cttagccgca
     4801 gttatacact ccgtataatt tccgacagcg ttttctgcaa ttatttcttc aagtttattt
     4861 tcgaaatttt cattagggtg catttcaaga acataatcac taataaatgc acgcgtctct
     4921 tgtttagctt tattactatc ttcgttatag ttaactaata tcattaactg atggtctatc
     4981 tctgataggt caacgtcata tttatccgca acggctttat atctttcagc atattcatat
     5041 ctaacatcat tagaatcatc ccacttaaag atgagaggaa tacctttttt ggccgcccac
     5101 tcaacaatat gatgactggt tgctgttaca tatttccgag gtccgcctgg cgtataagca
     5161 tggggattta cagatatttt agggaagcta taaaaatcgt tatctggatt acaatagcct
     5221 gttgttaaag catcgttaat gatttcataa cactcttcaa atagttgctg ttgatattca
     5281 accgggcgat taaaaaaatg catttcatct tttttttcgc aatcactaaa ccctaaaata
     5341 aatctccctt cacttaactg atccaataag caagcttcct ccgctatgcg gacaggatga
     5401 tgagttgtaa tgatgtgatt taatgaacca attttaattt tctctgttaa accgagcaga
     5461 aaaccagaaa cagtcagagg agcgccgaca acaccattat ctgaaaaatg attttcatac
     5521 actaaaatct gttcaaaatt caacttatca acatactccg ttatttcctg catgcgaact
     5581 atactctgtt cttgaacagt tgttgaattg atgaagttaa ggaagaacaa tccaaatttg
     5641 ctaccgccac cgccgctacc gccaccgccg ctaccgccac cgccgctacc gccaccgcca
     5701 taatataata gcgaacgttg tttttcttta agaaatggca tgacatcaga ctggaagagc
     5761 ttcatggaag caataatttc gtctactgtt ccattagctt caaatccaca acaaatattt
     5821 gatattcctg tagcatcaat gtctttttga attatgtcaa tacattcctg cggcgttccc
     5881 acgggattga tttcgtaact gtaatcaata cggcgattag tatctttatg tccttttaat
     5941 acaaagtcac gccactgccc tttattgaaa tcataacctc ttgtttggtc tgaatcatca
     6001 aaaatagtcg tagcattcac ataagaatca taccaatgcc ccagaaattt ccggcaaatc
     6061 tctttcgctt taattgagtc atgatctaca gatgttatat atgataagca atggtcgata
     6121 ttatgaatat cgtgcccata ttcttgagcc acttcattat aaagctcaag ttgtgctttc
     6181 ttttcgttag tatttataat ccaacttaat atcatcggta ggccaaattg agcagcccac
     6241 tcagtcgtcg aagctgattc agccaccaca taaaccggtg cgccacctct gctatacgcc
     6301 gcggggttta cttttacctt atggaacttg atatgttcat tatcagcttc catatatccc
     6361 tctgtcatgc cattctttat cagcccgtac cagcattccg ctaaggcgcg actgttattc
     6421 atatctgtgc cgaatacgcg aaagtccttg ttgtaaagcc ctcggcaaat accaaaccga
     6481 aatcgtcctt ttgacatttg atccaataaa ttcacatctt caagttggcg tactggatgg
     6541 gctgtgggaa gaacaatagc ggcagttcct acattcaatt ttttagtcgc gccaagtaaa
     6601 tatgcagcag cgacataagg gttaccaagc aaaccaaact ccgtgaaatg atgctccagt
     6661 aaccatacgg tatcaaaacc acactcctca gagatgcgac ctaatttaac caaacgtttc
     6721 attacctctg tttgggaaaa ttggggaggt tggtatgtaa gcaaaaagtt tccaaatttc
     6781 attaagactc cttattacgc agtatgttag caaacgtaga aaatacatac ataaaggtgg
     6841 caacatataa aagaaacgca aagacaccgc ggaacaggtt gatcttatcg cagtcgatac
     6901 tgaactcgta aggtttacca gcgccaaaga caaaagggcg acattcaacc gattgaggga
     6961 gggaaggtaa atattgacgg aaattattca ttaaaggtga attatcaccg tcaccgactt
     7021 gagccatttg ggaattagag ccagcaaaat caccagtagc accattacca ttagcaaggc
     7081 cggaaacgtc accaatgaaa ccatcgatag cagcaccgta atcagtagcg acagaatcaa
     7141 gtttgccttt agcgtcagac tgtagcgcgt tttcatcggc attttcggtc atagccccct
     7201 tattagcgtt tgccatcttt tcataatcaa aatcaccgga accagagcca ccaccggaac
     7261 cgcctccctc agagccgcca ccctcagaac cgccaccctc agagccacca ccctcagagc
     7321 cgccaccaga accaccacca gagccgccgc cagcattgac aggaggttga ggcaggtcag
     7381 acgattggcc ttgatattca caaacgaatg gatcctcatt aaagccagaa tggaaagcgc
     7441 agtctctgaa tttaccgttc cagtaagcgt catacatggc ttttgatgat acaggagtgt
     7501 actggtaata agttttaacg gggtcagtgc cttgagtaac agtgcccgta taaacagtta
     7561 atgccccctg cctatttcgg aacctattat tctgaaacat gaaagtatta agaggctgag
     7621 actcctcaag agaaggatta ggattagcgg ggttttgctc agtaccaggc ggataagtgc
     7681 cgtcgagagg gttgatataa gtatagcccg gaataggtgt atcaccgtac tcaggaggtt
     7741 tagtaccgcc accctcagaa ccgccaccct cagaaccgcc accctcagag ccaccaccct
     7801 cattttcagg gatagcaagc ccaataggaa cccatgtacc gtaacactga gtttcgtcac
     7861 cagtacaaac tacaacgcct gtagcattcc acagacagcc ctcatagtta gcgtaacgat
     7921 ctaaagtttt gtcgtctttc cagacgttag taaatgaatt ttctgtatgg ggttttgcta
     7981 aacaactttc aacagtttca gcggagtgag aatagaaagg aacaactaaa ggaattgcga
     8041 ataataattt tttcattttt ttttccttta ctgcacctgc aggtctttct tatgagttac
     8101 ctcctgaact tggtaaacag aacaacaaag ctgcctgata gtggccttta tttttggcat
     8161 aaataacaga ataaacactg cactgtgtat tcattccaac gagtgaatac acggagcaat
     8221 gtcgctcctt ctttctccct atagtgagtc gtattagtag ggtccctaag tctcctcagc
     8281 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgaccgg atgtcctctt
     8341 gacagtaaga cgggtaagcc tgttgatgat accgctgcct tactgggtgc attagccagt
     8401 ctgaatgacc tgtcacggga taatccgaag tggtcagact ggaaaatcag agggcaggaa
     8461 ctgctgaaca gcaaaaagtc agatagcacc acatagcaga cccgccataa aacgccctga
     8521 gaagcccgtg acgggctttt cttgtattat gggtagtttc cttgcatgaa tccataaaag
     8581 gcgcctgtag tgccatttac ccccattcac tgccagagcc gtgagcgcag cgaactgaat
     8641 gtcacgaaaa agacagcgac tcaggtgcct gatggtcgga gacaaaagga atattcagcg
     8701 atttgcccga gcttgcgagg gtgctactta agcctttagg gttttaaggt ctgttttgta
     8761 gaggagcaaa cagcgtttgc gacatccttt tgtaatactg cggaactgac taaagtagtg
     8821 agttatacac agggctggga tctattcttt ttatcttttt ttattctttc tttattctat
     8881 aaattataac cacttgaata taaacaaaaa aaacacacaa aggtctagcg gaatttacag
     8941 agggtctagc agaatttaca agttttccag caaaggtcta gcagaattta cagataccca
     9001 caactcaaag gaaaaggact agtaattatc attgactagc ccatctcaat tggtatagtg
     9061 attaaaatca cctagaccaa ttgagatgta tgtctgaatt agttgttttc aaagcaaatg
     9121 aactagcgat tagtcgctat gacttaacgg agcatgaaac caagctaatt ttatgctgtg
     9181 tggcactact caaccccacg attgaaaacc ctacaaggaa agaacggacg gtatcgttca
     9241 cttataacca atacgctcag atgatgaaca tcagtaggga aaatgcttat ggtgtattag
     9301 ctaaagcaac cagagagctg atgacgagaa ctgtggaaat caggaatcct ttggttaaag
     9361 gctttgagat tttccagtgg acaaactatg ccaagttctc aagcgaaaaa ttagaattag
     9421 tttttagtga agagatattg ccttatcttt tccagttaaa aaaattcata aaatataatc
     9481 tggaacatgt taagtctttt gaaaacaaat actctatgag gatttatgag tggttattaa
     9541 aagaactaac acaaaagaaa actcacaagg caaatataga gattagcctt gatgaattta
     9601 agttcatgtt aatgcttgaa aataactacc atgagtttaa aaggcttaac caatgggttt
     9661 tgaaaccaat aagtaaagat ttaaacactt acagcaatat gaaattggtg gttgataagc
     9721 gaggccgccc gactgatacg ttgattttcc aagttgaact agatagacaa atggatctcg
     9781 taaccgaact tgagaacaac cagataaaaa tgaatggtga caaaatacca acaaccatta
     9841 catcagattc ctacctacgt aacggactaa gaaaaacact acacgatgct ttaactgcaa
     9901 aaattcagct caccagtttt gaggcaaaat ttttgagtga catgcaaagt aagcatgatc
     9961 tcaatggttc gttctcatgg ctcacgcaaa aacaacgaac cacactagag aacatactgg
    10021 ctaaatacgg aaggatctga
//
