LOCUS       Exported               10051 bp ds-DNA     circular SYN 01-MAR-2021
DEFINITION  T3p-524-1264-proB.
ACCESSION   urn.local...3pc-d2b3lqy
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 2, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10051
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1184..1328
     modified_base   complement(1..16)
                     /label=USER junction
                     /note="USER junction"
     misc_feature    complement(3..15)
                     /label=SfiI
                     /note="SfiI"
                     /note="Keep unique in all constructs, can be used 
                     toexchange antibiotic resistance markers or plasmid 
                     origins"
     promoter        17..211
                     /note="bla promoter"
                     /note="from pUC19"
     CDS             212..1072
                     /label=bla (AmpR)
                     /note="bla (AmpR)"
                     /note="from pUC19"
     misc_feature    459..484
                     /label=MSP366
                     /note="MSP366"
     misc_feature    924..946
                     /label=534
                     /note="534"
     misc_feature    complement(1051..1094)
                     /label=MSP363
                     /note="MSP363"
     misc_feature    1073..1096
                     /label=MSP362
                     /note="MSP362"
     misc_feature    complement(1106..1133)
     terminator      1123..1167
                     /standard_name="rrnb"
                     /label=rrnb terminator
     misc_feature    complement(1150..1183)
                     /label=MSP383
                     /note="MSP383"
     misc_feature    1157..1188
                     /label=MSP384
                     /note="MSP384"
     misc_feature    1168..1328
     primer_bind     1168..1198
                     /standard_name="DB294"
                     /label=DB294
     primer_bind     complement(1168..1183)
                     /standard_name="AB371 primer bind"
                     /label=AB371 primer bind
     primer_bind     complement(1168..1183)
                     /standard_name="DB195"
                     /label=DB195
     misc_feature    1168..1183
                     /standard_name="USER junction"
                     /label=USER junction
     primer_bind     1210..1261
                     /label=AB365
                     /note="AB365"
     promoter        1233..1273
                     /standard_name="ProB"
                     /label=ProB promoter
                     /note="proD (Insulated Promoter - based on rrnB1 P1)"
     misc_feature    complement(1238..1259)
                     /label=MSP489
                     /note="MSP489"
     -35_signal      1239..1244
                     /label=-35
                     /note="-35"
     primer_bind     complement(1245..1296)
                     /label=AB366
                     /note="AB366"
     misc_feature    complement(1252..1287)
                     /label=MSP485-488
                     /note="MSP485-488"
     -10_signal      1262..1267
                     /label=-10
                     /note="-10"
     prim_transcript 1274
                     /label=+1
                     /note="+1"
     primer_bind     complement(1281..1328)
                     /standard_name="AB367"
                     /label=AB367
     misc_feature    complement(1293..1321)
                     /standard_name="TRB304"
                     /label=TRB304
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(1295..1336)
                     /label=MSP382
                     /note="MSP382"
     misc_feature    1306..1332
                     /standard_name="TRB305"
                     /label=TRB305
                     /note="Geneious type: PCR_primer"
     modified_base   1306..1328
     primer_bind     1306..1328
                     /standard_name="DB293"
                     /label=DB293
     misc_feature    1317..1354
                     /label=MSP381
                     /note="MSP381"
     RBS             1340..1354
                     /standard_name="sd9"
                     /label=sd9
     CDS             1355..1804
                     /standard_name="T7 lysozyme"
                     /label=T7 lysozyme
     misc_feature    1462..1487
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1258"
     misc_feature    1462..1487
                     /label=BCD1258
                     /note="BCD1258"
     misc_feature    complement(1784..1804)
                     /standard_name="MSP912"
                     /label=MSP912
                     /note="Geneious type: PCR_primer"
     misc_feature    1795..1936
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
     unsure          1795..1804
     CDS             1805..1834
                     /standard_name="Linker"
                     /label=Linker
     CDS             1835..1897
                     /standard_name="PTEN (20aa)"
                     /label=PTEN (20aa)
     CDS             1898..1927
                     /standard_name="Linker"
                     /label=Linker
     CDS             1928..4576
                     /standard_name="T3'-RNAP"
                     /label=T3'-RNAP
     misc_feature    1928..1952
                     /standard_name="TRB913"
                     /label=TRB913
                     /note="Geneious type: PCR_primer"
     unsure          1928..1936
     misc_feature    1938..4250
                     /standard_name="TCTGACCGTACCTCTAACCGTCGTCTGCAGCAGACCCAGGCT 
                     CAGGTTGAAGAAGTTGTTGACATCATCCGTGTTAACGTTGACAAAGTTCTGGAACGTG 
                     GCCAGAAACTGTCTGAACTGGACGACCGTGCTGACGCTCTGCAGGCTGGTGCTTCTCA 
                     GTTCGAATCTTCTGCTGCTAAACTGAAACGTAACACGATTAACATCGCTAAGAACGAC 
                     TTCTCTGACATCGAACTGGCTGCTATCCCGTTCAACACTCTGGCTGACCATTACGGTG 
                     AGCGTTTAGCTCGCGAACAGTTGGCCCTTGAGCATGAGTCTTACGAGATGGGTGAAGC 
                     ACGCTTCCGCAAGATGTTTGAGCGTCAACTTAAAGCTGGTGAGGTTGCGGATAACGCT 
                     GCCGCCAAGCCTCTCATCACTACCCTACTCCCTAAGATGATTGCACGCATCAACGACT 
                     GGTTTGAGGAAGTGAAAGCTAAGCGCGGCAAGCGCCCGACAGCCTTCCAGTTCCTGCA 
                     AGAAATCAAGCCGGAAGCCGTAGCGTACATCACCATTAAGACCACTCTGGCTTGCCTA 
                     ACCAGTGCTGACAATACAACCGTTCAGGCTGTAGCAAGCGCAATCGGTCGGGCCATTG 
                     AGGACGAGGCTCGCTTCGGTCGTATCCGTGACCTTGAAGCTAAGCACTTCAAGAAAAA 
                     CGTTGAGGAACAACTCAACAAGCGCGTAGGGCACGTCTACAAGAAAGCATTTATGCAA 
                     GTTGTCGAGGCTGACATGCTCTCTAAGGGTCTACTCGGTGGCGAGGCGTGGTCTTCGT 
                     GGCATAAGGAAGACTCTATTCATGTAGGAGTACGCTGCATCGAGATGCTCATTGAGTC 
                     AACCGGAATGGTTAGCTTACACCGCCAAAATGCTGGCGTAGTAGGTCAAGACTCTGAG 
                     ACTATCGAACTCGCACCTGAATACGCTGAGGCTATCGCAACCCGTGCAGGTGCGCTGG 
                     CTGGCATCTCTCCGATGTTCCAACCTTGCGTAGTTCCTCCTAAGCCGTGGACTGGCAT 
                     TACTGGTGGTGGCTATTGGGCTAACGGTCGTCGTCCTCTGGCGCTGGTGCGTACTCAC 
                     AGTAAGAAAGCACTGATGCGCTACGAAGACGTTTACATGCCTGAGGTGTACAAAGCGA 
                     TTAACATTGCGCAAAACACCGCATGGAAAATCAACAAGAAAGTCCTAGCGGTCGCCAA 
                     CGTAATCACCAAGTGGAAGCATTGTCCGGTCGAGGACATCCCTGCGATTGAGCGTGAA 
                     GAACTCCCGATGAAACCGGAAGACATCGACATGAATCCTGAGGCTCTCACCGCGTGGA 
                     AACGTGCTGCCGCTGCTGTGTACCGCAAGGACAAGGCTCGCAAGTCTCGCCGTATCAG 
                     CCTTGAGTTCATGCTTGAGCAAGCCAATAAGTTTGCTAACCATAAGGCCATCTGGTTC 
                     CCTTACAACATGGACTGGCGCGGTCGTGTTTACGCTGTGTCAATGTTCAACCCGCAAG 
                     GTAACGATATGACCAAAGGACTGCTTACGCTGGCGAAAGGTAAACCAATCGGTAAGGA 
                     AGGTTACTACTGGCTGAAAATCCACGGTGCAAACTGTGCGGGTGTCGATAAGGTTCCG 
                     TTCCCTGAGCGCATCAAGTTCATTGAGGAAAACCACGAGAACATCATGGCTTGCGCTA 
                     AGTCTCCACTGGAGAACACTTGGTGGGCTGAGCAAGATTCTCCGTTCTGCTTCCTTGC 
                     GTTCTGCTTTGAGTACGCTGGGGTACAGCACCACGGCCTGAGCTATAACTGCTCCCTT 
                     CCGCTGGCGTTTGACGGGTCTTGCTCTGGCATCCAGCACTTCTCCGCGATGCTCCGAG 
                     ATGAGGTAGGTGGTCGCGCGGTTAACTTGCTTCCTAGTGAAACCGTTCAGGACATCTA 
                     CGGGATTGTTGCTAAGAAAGTCAACGAGATTCTACAAGCAGACGCAATCAATGGGACC 
                     GATAACGAAGTAGTTACCGTGACCGATGAGAACACTGGTGAAATCTCTGAGAAAGTCA 
                     AGCTGGGCACTAAGGCACTGGCTGGTCAATGGCTGGCTTACGGTGTTACTCGCAGTGT 
                     GACTAAGCGTTCAGTCATGACGCTGGCTTACGGGTCCAAAGAGTTCGGCTTCCGTCAA 
                     CAAGTGCTGGAAGATACCATTCAGCCAGCTATTGATTCCGGCAAGGGTCTGATGTTCA 
                     CTCAGCCGAATCAGGCTGCTGGATACATGGCTAAGCTGATTTGGGAATCTGTGAGCGT 
                     GACGGTGGTAGCTGCGGTTGAAGCAATGAACTGGCTTAAGTCTGCTGCTAAGCTGCTG 
                     GCTGCTGAGGTCAAAGATAAGAAGACTGGAGAGATTCTTCGCAAGCGTTGCGCTGTGC 
                     ATTGGGTAACTCCTGATGGTTTCCCTGTGTGGCAGGAATACAAGAAGCCTATTCAGAC 
                     GCGCTTGAACCTGATGTTCCTCGGTCAGTTCCGCTTACAGCCTACCATTAACACCAAC 
                     AAAGATAGCGAGATTGATGCACACAAACAGGAGT"
                     /label=Geneious type: Editing History Replacement
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2182..2208
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1253"
     misc_feature    2182..2208
                     /label=1253
                     /note="1253"
     misc_feature    2210..2212
                     /label=R96L
                     /note="R96L"
     misc_feature    2216..2218
                     /label=K98R
                     /note="K98R"
     misc_feature    2235..2254
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1259"
     misc_feature    2235..2254
                     /label=1259
                     /note="1259"
     misc_feature    2364..2397
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD710"
     misc_feature    2537..2539
                     /label=H205Y
                     /note="H205Y"
     misc_feature    2543..2545
                     /label=E207K
                     /note="E207K"
     misc_feature    complement(2554..2576)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="bcd1175"
     misc_feature    2561..2578
                     /standard_name="SeqT7-1"
                     /label=SeqT7-1
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(2564..2593)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1252"
     misc_feature    complement(2564..2593)
                     /label=1252
                     /note="1252"
     misc_feature    2588..2590
                     /label=E222K
                     /note="E222K"
     misc_feature    2631..2660
                     /label=t7seq2b
                     /note="t7seq2b"
     misc_feature    2715..2743
                     /label=T7seq2
                     /note="T7seq2"
     misc_feature    complement(2878..2909)
                     /label=T7seq1
                     /note="T7seq1"
     misc_feature    2979..3000
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1260"
     misc_feature    2979..3000
                     /label=1260
                     /note="1260"
     misc_feature    3113..3115
                     /label=S397R
                     /note="S397R"
     misc_feature    3230..3259
                     /label=t7seq2c
                     /note="t7seq2c"
     misc_feature    3241..3275
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="bcd711"
     misc_feature    3310..3328
                     /standard_name="SeqT7-2"
                     /label=SeqT7-2
                     /note="Geneious type: PCR_primer"
     misc_feature    3548..3550
                     /label=G542V
                     /note="G542V"
     misc_feature    3613..3640
                     /label=T7 seq3
                     /note="T7 seq3"
     misc_feature    3686..3708
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCd1261"
     misc_feature    3686..3708
                     /label=1261
                     /note="1261"
     misc_feature    complement(3686..3708)
                     /label=MSP347
                     /note="MSP347"
     misc_feature    3830..3859
                     /label=T7seq3b
                     /note="T7seq3b"
     misc_feature    3878..3880
                     /label=E652D
                     /note="E652D"
     misc_feature    3893..3913
                     /label=MSP63
                     /note="MSP63"
     misc_feature    3914..3934
                     /label=MSP62
                     /note="MSP62"
     misc_feature    3947..3949
                     /label=G675R
                     /note="G675R"
     misc_feature    4106..4108
                     /label=V728I
                     /note="V728I"
     misc_feature    4166..4168
                     /label=N748D
                     /note="N748D"
     misc_feature    4199..4201
                     /label=P759L
                     /note="P759L"
     misc_feature    4250..4252
                     /label=S776A
                     /note="S776A"
     misc_feature    4256..4278
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1262"
     misc_feature    4256..4278
                     /label=1262
                     /note="1262"
     misc_feature    complement(4541..4562)
                     /label=MSP997
                     /note="MSP997"
     misc_feature    4552..4573
                     /label=MSP996
                     /note="MSP996"
     misc_feature    complement(4555..4592)
                     /standard_name="TRB301"
                     /label=TRB301
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(4557..4576)
                     /label=MSP346
                     /note="MSP346"
     misc_feature    4569..4576
                     /label=MSP20
                     /note="MSP20"
     misc_feature    4577..4615
                     /label=MSP364
                     /note="MSP364"
     misc_feature    4577..4615
                     /label=364
                     /note="364"
     misc_feature    4577..4593
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(4577..4593)
                     /label=MSP346
                     /note="MSP346"
     misc_feature    complement(4577..4593)
                     /label=346
                     /note="346"
     misc_feature    4577..4593
                     /label=MSP20
                     /note="MSP20"
     misc_feature    complement(4577..4593)
                     /label=MSP21
                     /note="MSP21"
     misc_feature    4582..4604
                     /standard_name="TRB306"
                     /label=TRB306
                     /note="Geneious type: PCR_primer"
     CDS             4591..4593
                     /standard_name="III fragment"
                     /label=III fragment
     misc_feature    4594..4623
                     /label=1042
                     /note="1042"
     modified_base   complement(4594..4606)
                     /label=USER junction
                     /note="USER junction"
     terminator      4607..4648
                     /note="tetA/orfL bidirectional terminator"
                     /note="termination of bla transcript is slightly weakerthan
                     opposite direction"
     misc_feature    complement(4649..4655)
                     /label=Nt-BbvCI
                     /note="Nt-BbvCI"
                     /note="nicking endonuclease site that generates overhang"
     modified_base   complement(4656..6795)
                     /label=USER junction
                     /note="USER junction"
     misc_feature    complement(4663..4670)
                     /label=PacI
                     /note="PacI"
                     /note="Keep unique in all constructs, can be used 
                     toexchange antibiotic resistance marker or selection 
                     components"
     CDS             complement(4673..6796)
                     /label=xluxAB
                     /note="xluxAB"
     misc_feature    complement(5311..5339)
                     /label=708
                     /note="708"
     misc_feature    5332..5362
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP14"
     misc_feature    complement(5332..5362)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP15"
     sig_peptide     complement(5654..5713)
                     /note="GGGGS4 flexible linker"
     misc_feature    complement(6093..6124)
                     /label=707
                     /note="707"
     misc_feature    complement(6742..6768)
                     /label=MSP497
                     /note="MSP497"
     misc_feature    complement(6761..6787)
                     /label=MSP498
                     /note="MSP498"
     CDS             complement(6796..8070)
                     /standard_name="III"
                     /label=III
     misc_feature    6796..6825
                     /label=MSP64
                     /note="MSP64"
     misc_feature    complement(6883..6928)
                     /label=modified to remove internal promoter
                     /note="modified to remove internal promoter"
     misc_feature    complement(6907..6940)
                     /label=706
                     /note="706"
     misc_feature    complement(7671..7700)
                     /label=705
                     /note="705"
     RBS             complement(8071..8080)
                     /standard_name="RBS"
                     /note="RBS sd8"
     misc_feature    complement(8082..8087)
                     /label=BsgI
                     /note="BsgI"
     misc_feature    complement(8087..8094)
                     /label=SbfI
                     /note="SbfI"
     terminator      complement(8095..8240)
                     /standard_name="T1 Terminator"
                     /label=T1 Terminator
     misc_difference 8228..8288
     misc_feature    complement(8232..8263)
                     /standard_name="MSP1001"
                     /label=MSP1001
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(8248..8267)
                     /label=T3 promoter
                     /note="T3 promoter"
                     /note="Geneious type: promoter"
     misc_feature    8252..8267
                     /standard_name="ATAGTGAGTCGTATTA"
                     /label=ATAGTGAGTCGTATTA
                     /note="Geneious type: Editing History Replacement"
     misc_feature    8254..8288
                     /standard_name="MSP1000"
                     /label=MSP1000
                     /note="Geneious type: PCR_primer"
     modified_base   complement(8270..8284)
                     /label=USER junction
                     /note="USER junction"
     misc_feature    complement(8271..8277)
                     /label=SanDI
                     /note="SanDI"
                     /note="Keep unique in all constructs, can be used 
                     toexchange f1 origin or selection components"
     misc_feature    complement(8285..8291)
                     /label=Nt-BbvCI
                     /note="Nt-BbvCI"
                     /note="nicking endonuclease site that generates overhang"
     terminator      complement(8292..8336)
                     /note="rrnB1 transcriptional terminator"
     modified_base   complement(8337..8351)
                     /label=USER junction
                     /note="USER junction"
     misc_feature    8350..8376
                     /label=1044
                     /note="1044"
     rep_origin      8352..9097
     misc_feature    complement(8458..8484)
                     /label=535
                     /note="535"
     misc_feature    9082..9125
                     /label=814
                     /note="814"
     CDS             9101..10051
                     /label=RepA
                     /note="RepA"
                     /note="pSC101 origin of replication protein"
     misc_feature    9852..9875
                     /label=MSP365
                     /note="MSP365"
ORIGIN
        1 acggcctatt tggcctaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
       61 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      121 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      181 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      241 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      301 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      361 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      421 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      481 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      541 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      601 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      661 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
      721 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
      781 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
      841 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
      901 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
      961 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1021 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aacgtaatgc
     1081 tctgccagtg ttacaaccaa ttaaccaatt ctgatttccg gtcaaataaa acgaaaggct
     1141 cagtcgaaag actgggcctt tcgttttatt ctagagcaca gctaacacca cgtcgtccct
     1201 atctgctgcc ctaggtctat gagtggttgc tggataactt tacgggcatg cataaggctc
     1261 gtaatatata ttcagggaga ccacaacggt ttccctctac aaataatttt gtttaacttt
     1321 tactagagtt ccttcctcga aaggaaaaaa aaaaatggct cgtgtacagt ttaaacaacg
     1381 tgaatctact gacgcaatct ttgttcactg ctcggctacc aagccaagtc agaatgttgg
     1441 tgtccgtgag attcgccagt ggcacaaaga gcagggttgg ctcgatgtgg gataccactt
     1501 tatcatcaag cgagacggta ctgtggaggc aggacgagat gagatggctg taggctctca
     1561 cgctaagggt tacaaccaca actctatcgg cgtctgcctt gttggtggta tcgacgataa
     1621 aggtaagttc gacgctaact ttacgccagc ccaaatgcaa tcccttcgct cactgcttgt
     1681 cacactgctg gctaagtacg aaggcgctgt gcttcgcgcc catcatgagg tggcgccgaa
     1741 ggcttcccct tcgttcgacc ttaagcgttg gtgggagaag aacgaactgg tcacatctga
     1801 ccgtggtagc ggcggtggtg cgagtggtgg tgcggagaac ggttccttat gcgaccaaga
     1861 gatcgattcg atctgcagta tcgagagagc agataacggc ggtagcgcag gcagtggagc
     1921 gggcggtaac acgattaaca tcgctaagaa cgacttctct gacatcgaac tggctgctat
     1981 cccgttcaac actctggctg accattacgg tgagcgttta gctcgcgaac agttggccct
     2041 tgagcatgag tcttacgaga tgggtgaagc acgcttccgc aagatgtttg agcgtcaact
     2101 taaagctggt gaggttgcgg ataacgctgc cgccaagcct ctcatcacta ccctactccc
     2161 taagatgatt gcacgcatca acgactggtt tgaggaagtg aaagctaagc tcggcaggcg
     2221 cccgacagcc ttccagttcc tgcaagaaat caagccggaa gccgtagcgt acatcaccat
     2281 taagaccact ctggcttgcc taaccagtgc tgacaataca accgttcagg ctgtagcaag
     2341 cgcaatcggt cgggccattg aggacgaggc tcgcttcggt cgtatccgtg accttgaagc
     2401 taagcacttc aagaaaaacg ttgaggaaca actcaacaag cgcgtagggc acgtctacaa
     2461 gaaagcattt atgcaagttg tcgaggctga catgctctct aagggtctac tcggtggcga
     2521 ggcgtggtct tcgtggtata agaaagactc tattcatgta ggagtacgct gcatcgagat
     2581 gctcattaag tcaactggaa tggttagctt acaccgccaa aatgctggcg tagtaggtca
     2641 agactctgag actatcgaac tcgcacctga atacgctgag gctatcgcaa cccgtgcagg
     2701 tgcgctggct ggcatctctc cgatgttcca accttgcgta gttcctccta agccgtggac
     2761 tggcattact ggtggtggct attgggctaa cggtcgtcgt cctctggcgc tggtgcgtac
     2821 tcacagtaag aaagcactga tgcgctacga agacgtttac atgcctgagg tgtacaaagc
     2881 gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag aaagtcctag cggtcgccaa
     2941 cgtaatcacc aagtggaagc attgtccggt cgaggacatc cctgcgattg agcgtgaaga
     3001 actcccgatg aaaccggaag acatcgacat gaatcctgag gctctcaccg cgtggaaacg
     3061 tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag tctcgccgta tcagacttga
     3121 gttcatgctt gagcaagcca ataagtttgc taaccataag gccatctggt tcccttacaa
     3181 catggactgg cgcggtcgtg tttacgctgt gtcaatgttc aacccgcaag gtaacgatat
     3241 gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc ggtaaggaag gttactactg
     3301 gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag gttccgttcc ctgagcgcat
     3361 caagttcatt gaggaaaacc acgagaacat catggcttgc gctaagtctc cactggagaa
     3421 cacttggtgg gctgagcaag attctccgtt ctgcttcctt gcgttctgct ttgagtacgc
     3481 tggggtacag caccacggcc tgagctataa ctgctccctt ccgctggcgt ttgacgggtc
     3541 ttgctctgtc atccagcact tctccgcgat gctccgagat gaggtaggtg gtcgcgcggt
     3601 taacttgctt cctagtgaaa ccgttcagga catctacggg attgttgcta agaaagtcaa
     3661 cgagattcta caagcagacg caatcaatgg gaccgataac gaagtagtta ccgtgaccga
     3721 tgagaacact ggtgaaatct ctgagaaagt caagctgggc actaaggcac tggctggtca
     3781 atggctggct tacggtgtta ctcgcagtgt gactaagcgt tcagtcatga cgctggctta
     3841 cgggtccaaa gagttcggct tccgtcaaca agtgctggac gataccattc agccagctat
     3901 tgattccggc aagggtctga tgttcaccca gccgaatcag gctgctagat acatggctaa
     3961 gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg gttgaagcaa tgaactggct
     4021 taagtctgct gctaagctgc tggctgctga ggtcaaagat aagaagactg gagagattct
     4081 tcgcaagcgt tgcgctgtgc attggataac tcctgatggt ttccctgtgt ggcaggaata
     4141 caagaagcct attcagacgc gcttggacct gatgttcctc ggtcagttcc gcttacagct
     4201 taccattaac accaacaaag atagcgagat tgatgcacac aaacaggagg ctggtatcgc
     4261 tcctaacttt gtacacagcc aagacggtag ccaccttcgt aagactgtag tgtgggcaca
     4321 cgagaagtac ggaatcgaat cttttgcact gattcacgac tccttcggta ccattccggc
     4381 tgacgctgcg aacctgttca aagcagtgcg cgaaactatg gttgacacat atgagtcttg
     4441 tgatgtactg gctgatttct acgaccagtt cgctgaccag ttgcacgagt ctcaattgga
     4501 caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc cgtgacatct tagagtcgga
     4561 cttcgcgttc gcgtaatgga gattttcaac atgacgccat gggcattaaa aaatgccctc
     4621 ttgggttatc aagagggtca ttatatttgc tgaggagtgc cgttaattaa gtttaggtat
     4681 attccgtgtg gtacttctta atattatcat caacaatatt gattacattt ttttggctca
     4741 tcaaatcatt cattggttca aaggacagca atacactttt cgcaccacac ttttcaattg
     4801 ccaacttagc cgcagttata cactccgtat aatttccgac agcgttttct gcaattattt
     4861 cttcaagttt attttcgaaa ttttcattag ggtgcatttc aagaacataa tcactaataa
     4921 atgcacgcgt ctcttgttta gctttattac tatcttcgtt atagttaact aatatcatta
     4981 actgatggtc tatctctgat aggtcaacgt catatttatc cgcaacggct ttatatcttt
     5041 cagcatattc atatctaaca tcattagaat catcccactt aaagatgaga ggaatacctt
     5101 ttttggccgc ccactcaaca atatgatgac tggttgctgt tacatatttc cgaggtccgc
     5161 ctggcgtata agcatgggga tttacagata ttttagggaa gctataaaaa tcgttatctg
     5221 gattacaata gcctgttgtt aaagcatcgt taatgatttc ataacactct tcaaatagtt
     5281 gctgttgata ttcaaccggg cgattaaaaa aatgcatttc atcttttttt tcgcaatcac
     5341 taaaccctaa aataaatctc ccttcactta actgatccaa taagcaagct tcctccgcta
     5401 tgcggacagg atgatgagtt gtaatgatgt gatttaatga accaatttta attttctctg
     5461 ttaaaccgag cagaaaacca gaaacagtca gaggagcgcc gacaacacca ttatctgaaa
     5521 aatgattttc atacactaaa atctgttcaa aattcaactt atcaacatac tccgttattt
     5581 cctgcatgcg aactatactc tgttcttgaa cagttgttga attgatgaag ttaaggaaga
     5641 acaatccaaa tttgctaccg ccaccgccgc taccgccacc gccgctaccg ccaccgccgc
     5701 taccgccacc gccataatat aatagcgaac gttgtttttc tttaagaaat ggcatgacat
     5761 cagactggaa gagcttcatg gaagcaataa tttcgtctac tgttccatta gcttcaaatc
     5821 cacaacaaat atttgatatt cctgtagcat caatgtcttt ttgaattatg tcaatacatt
     5881 cctgcggcgt tcccacggga ttgatttcgt aactgtaatc aatacggcga ttagtatctt
     5941 tatgtccttt taatacaaag tcacgccact gccctttatt gaaatcataa cctcttgttt
     6001 ggtctgaatc atcaaaaata gtcgtagcat tcacataaga atcataccaa tgccccagaa
     6061 atttccggca aatctctttc gctttaattg agtcatgatc tacagatgtt atatatgata
     6121 agcaatggtc gatattatga atatcgtgcc catattcttg agccacttca ttataaagct
     6181 caagttgtgc tttcttttcg ttagtattta taatccaact taatatcatc ggtaggccaa
     6241 attgagcagc ccactcagtc gtcgaagctg attcagccac cacataaacc ggtgcgccac
     6301 ctctgctata cgccgcgggg tttactttta ccttatggaa cttgatatgt tcattatcag
     6361 cttccatata tccctctgtc atgccattct ttatcagccc gtaccagcat tccgctaagg
     6421 cgcgactgtt attcatatct gtgccgaata cgcgaaagtc cttgttgtaa agccctcggc
     6481 aaataccaaa ccgaaatcgt ccttttgaca tttgatccaa taaattcaca tcttcaagtt
     6541 ggcgtactgg atgggctgtg ggaagaacaa tagcggcagt tcctacattc aattttttag
     6601 tcgcgccaag taaatatgca gcagcgacat aagggttacc aagcaaacca aactccgtga
     6661 aatgatgctc cagtaaccat acggtatcaa aaccacactc ctcagagatg cgacctaatt
     6721 taaccaaacg tttcattacc tctgtttggg aaaattgggg aggttggtat gtaagcaaaa
     6781 agtttccaaa tttcattaag actccttatt acgcagtatg ttagcaaacg tagaaaatac
     6841 atacataaag gtggcaacat ataaaagaaa cgcaaagaca ccgcggaaca ggttgatctt
     6901 atcgcagtcg atactgaact cgtaaggttt accagcgcca aagacaaaag ggcgacattc
     6961 aaccgattga gggagggaag gtaaatattg acggaaatta ttcattaaag gtgaattatc
     7021 accgtcaccg acttgagcca tttgggaatt agagccagca aaatcaccag tagcaccatt
     7081 accattagca aggccggaaa cgtcaccaat gaaaccatcg atagcagcac cgtaatcagt
     7141 agcgacagaa tcaagtttgc ctttagcgtc agactgtagc gcgttttcat cggcattttc
     7201 ggtcatagcc cccttattag cgtttgccat cttttcataa tcaaaatcac cggaaccaga
     7261 gccaccaccg gaaccgcctc cctcagagcc gccaccctca gaaccgccac cctcagagcc
     7321 accaccctca gagccgccac cagaaccacc accagagccg ccgccagcat tgacaggagg
     7381 ttgaggcagg tcagacgatt ggccttgata ttcacaaacg aatggatcct cattaaagcc
     7441 agaatggaaa gcgcagtctc tgaatttacc gttccagtaa gcgtcataca tggcttttga
     7501 tgatacagga gtgtactggt aataagtttt aacggggtca gtgccttgag taacagtgcc
     7561 cgtataaaca gttaatgccc cctgcctatt tcggaaccta ttattctgaa acatgaaagt
     7621 attaagaggc tgagactcct caagagaagg attaggatta gcggggtttt gctcagtacc
     7681 aggcggataa gtgccgtcga gagggttgat ataagtatag cccggaatag gtgtatcacc
     7741 gtactcagga ggtttagtac cgccaccctc agaaccgcca ccctcagaac cgccaccctc
     7801 agagccacca ccctcatttt cagggatagc aagcccaata ggaacccatg taccgtaaca
     7861 ctgagtttcg tcaccagtac aaactacaac gcctgtagca ttccacagac agccctcata
     7921 gttagcgtaa cgatctaaag ttttgtcgtc tttccagacg ttagtaaatg aattttctgt
     7981 atggggtttt gctaaacaac tttcaacagt ttcagcggag tgagaataga aaggaacaac
     8041 taaaggaatt gcgaataata attttttcat tttttccttt actgcacctg caggtctttc
     8101 ttatgagtta cctcctgaac ttggtaaaca gaacaacaaa gctgcctgat agtggccttt
     8161 atttttggca taaataacag aataaacact gcactgtgta ttcattccaa cgagtgaata
     8221 cacggagcaa tgtcgctcct tctttctccc tttagtgagg gttaattgta gggtccctaa
     8281 gtctcctcag caaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgaccg
     8341 gatgtcctct tgacagtaag acgggtaagc ctgttgatga taccgctgcc ttactgggtg
     8401 cattagccag tctgaatgac ctgtcacggg ataatccgaa gtggtcagac tggaaaatca
     8461 gagggcagga actgctgaac agcaaaaagt cagatagcac cacatagcag acccgccata
     8521 aaacgccctg agaagcccgt gacgggcttt tcttgtatta tgggtagttt ccttgcatga
     8581 atccataaaa ggcgcctgta gtgccattta cccccattca ctgccagagc cgtgagcgca
     8641 gcgaactgaa tgtcacgaaa aagacagcga ctcaggtgcc tgatggtcgg agacaaaagg
     8701 aatattcagc gatttgcccg agcttgcgag ggtgctactt aagcctttag ggttttaagg
     8761 tctgttttgt agaggagcaa acagcgtttg cgacatcctt ttgtaatact gcggaactga
     8821 ctaaagtagt gagttataca cagggctggg atctattctt tttatctttt tttattcttt
     8881 ctttattcta taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc
     8941 ggaatttaca gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt
     9001 acagataccc acaactcaaa ggaaaaggac tagtaattat cattgactag cccatctcaa
     9061 ttggtatagt gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt
     9121 caaagcaaat gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat
     9181 tttatgctgt gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac
     9241 ggtatcgttc acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta
     9301 tggtgtatta gctaaagcaa ccagagagct gatgacgaga actgtggaaa tcaggaatcc
     9361 tttggttaaa ggctttgaga ttttccagtg gacaaactat gccaagttct caagcgaaaa
     9421 attagaatta gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat
     9481 aaaatataat ctggaacatg ttaagtcttt tgaaaacaaa tactctatga ggatttatga
     9541 gtggttatta aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct
     9601 tgatgaattt aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa
     9661 ccaatgggtt ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt
     9721 ggttgataag cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca
     9781 aatggatctc gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc
     9841 aacaaccatt acatcagatt cctacctacg taacggacta agaaaaacac tacacgatgc
     9901 tttaactgca aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag
     9961 taagcatgat ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa ccacactaga
    10021 gaacatactg gctaaatacg gaaggatctg a
//
